Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS08380 and MMJJ_RS08385 are separated by 20 nucleotides MMJJ_RS08385 and MMJJ_RS08390 are separated by 13 nucleotides MMJJ_RS08390 and MMJJ_RS08395 are separated by 33 nucleotides MMJJ_RS08395 and MMJJ_RS08400 are separated by 12 nucleotides MMJJ_RS08400 and MMJJ_RS08405 are separated by 227 nucleotides
MMJJ_RS08380: MMJJ_RS08380 - hypothetical protein, at 1,543,915 to 1,544,181
_RS08380
MMJJ_RS08385: MMJJ_RS08385 - class II SORL domain-containing protein, at 1,544,202 to 1,544,558
_RS08385
MMJJ_RS08390: MMJJ_RS08390 - carboxymuconolactone decarboxylase family protein, at 1,544,572 to 1,544,886
_RS08390
MMJJ_RS08395: MMJJ_RS08395 - CoB--CoM heterodisulfide reductase subunit B, at 1,544,920 to 1,545,825
_RS08395
MMJJ_RS08400: MMJJ_RS08400 - CoB--CoM heterodisulfide reductase subunit C, at 1,545,838 to 1,546,416
_RS08400
MMJJ_RS08405: MMJJ_RS08405 - nickel-dependent hydrogenase large subunit, at 1,546,644 to 1,547,771
_RS08405
Position (kb)
1544
1545
1546 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 1543.920 kb on - strand at 1543.921 kb on + strand at 1543.921 kb on + strand at 1543.936 kb on + strand at 1543.990 kb on - strand, within MMJJ_RS08380 at 1544.018 kb on + strand at 1544.018 kb on + strand, within MMJJ_RS08380 at 1544.018 kb on + strand, within MMJJ_RS08380 at 1544.090 kb on + strand, within MMJJ_RS08380 at 1544.313 kb on + strand, within MMJJ_RS08385 at 1544.317 kb on + strand, within MMJJ_RS08385 at 1544.320 kb on + strand, within MMJJ_RS08385 at 1544.347 kb on + strand, within MMJJ_RS08385 at 1544.358 kb on - strand, within MMJJ_RS08385 at 1544.376 kb on - strand, within MMJJ_RS08385 at 1544.377 kb on + strand, within MMJJ_RS08385 at 1544.382 kb on - strand, within MMJJ_RS08385 at 1544.382 kb on - strand, within MMJJ_RS08385 at 1544.382 kb on - strand, within MMJJ_RS08385 at 1544.383 kb on + strand, within MMJJ_RS08385 at 1544.391 kb on + strand, within MMJJ_RS08385 at 1544.428 kb on + strand, within MMJJ_RS08385 at 1544.448 kb on - strand, within MMJJ_RS08385 at 1544.449 kb on + strand, within MMJJ_RS08385 at 1544.491 kb on + strand, within MMJJ_RS08385 at 1544.547 kb on - strand at 1544.559 kb on - strand at 1544.559 kb on - strand at 1544.560 kb on + strand at 1544.560 kb on + strand at 1544.587 kb on - strand at 1544.587 kb on - strand at 1544.588 kb on + strand at 1544.604 kb on - strand, within MMJJ_RS08390 at 1544.627 kb on + strand, within MMJJ_RS08390 at 1544.629 kb on - strand, within MMJJ_RS08390 at 1544.633 kb on + strand, within MMJJ_RS08390 at 1544.790 kb on + strand, within MMJJ_RS08390 at 1544.823 kb on + strand, within MMJJ_RS08390 at 1544.916 kb on - strand at 1544.921 kb on - strand at 1544.921 kb on - strand at 1544.922 kb on + strand at 1544.922 kb on + strand at 1544.922 kb on + strand at 1544.928 kb on - strand at 1544.932 kb on - strand at 1544.932 kb on - strand at 1545.003 kb on - strand at 1545.032 kb on - strand, within MMJJ_RS08395 at 1545.121 kb on - strand, within MMJJ_RS08395 at 1545.212 kb on + strand, within MMJJ_RS08395 at 1545.237 kb on - strand, within MMJJ_RS08395 at 1545.238 kb on + strand, within MMJJ_RS08395 at 1545.249 kb on - strand, within MMJJ_RS08395 at 1545.250 kb on + strand, within MMJJ_RS08395 at 1545.251 kb on - strand, within MMJJ_RS08395 at 1545.251 kb on - strand, within MMJJ_RS08395 at 1545.251 kb on - strand, within MMJJ_RS08395 at 1545.251 kb on - strand, within MMJJ_RS08395 at 1545.251 kb on - strand, within MMJJ_RS08395 at 1545.251 kb on - strand, within MMJJ_RS08395 at 1545.252 kb on + strand, within MMJJ_RS08395 at 1545.252 kb on + strand, within MMJJ_RS08395 at 1545.252 kb on + strand, within MMJJ_RS08395 at 1545.252 kb on + strand, within MMJJ_RS08395 at 1545.254 kb on - strand, within MMJJ_RS08395 at 1545.254 kb on - strand, within MMJJ_RS08395 at 1545.254 kb on - strand, within MMJJ_RS08395 at 1545.254 kb on - strand, within MMJJ_RS08395 at 1545.254 kb on - strand, within MMJJ_RS08395 at 1545.255 kb on + strand, within MMJJ_RS08395 at 1545.255 kb on + strand, within MMJJ_RS08395 at 1545.257 kb on + strand, within MMJJ_RS08395 at 1545.321 kb on - strand, within MMJJ_RS08395 at 1545.322 kb on + strand, within MMJJ_RS08395 at 1545.329 kb on + strand, within MMJJ_RS08395 at 1545.337 kb on + strand, within MMJJ_RS08395 at 1545.337 kb on + strand, within MMJJ_RS08395 at 1545.343 kb on - strand, within MMJJ_RS08395 at 1545.343 kb on - strand, within MMJJ_RS08395 at 1545.344 kb on + strand, within MMJJ_RS08395 at 1545.344 kb on + strand, within MMJJ_RS08395 at 1545.347 kb on - strand, within MMJJ_RS08395 at 1545.359 kb on - strand, within MMJJ_RS08395 at 1545.431 kb on - strand, within MMJJ_RS08395 at 1545.432 kb on + strand, within MMJJ_RS08395 at 1545.436 kb on - strand, within MMJJ_RS08395 at 1545.436 kb on - strand, within MMJJ_RS08395 at 1545.436 kb on - strand, within MMJJ_RS08395 at 1545.472 kb on + strand, within MMJJ_RS08395 at 1545.473 kb on - strand, within MMJJ_RS08395 at 1545.473 kb on - strand, within MMJJ_RS08395 at 1545.473 kb on - strand, within MMJJ_RS08395 at 1545.473 kb on - strand, within MMJJ_RS08395 at 1545.505 kb on - strand, within MMJJ_RS08395 at 1545.505 kb on - strand, within MMJJ_RS08395 at 1545.505 kb on - strand, within MMJJ_RS08395 at 1545.510 kb on - strand, within MMJJ_RS08395 at 1545.510 kb on - strand, within MMJJ_RS08395 at 1545.510 kb on - strand, within MMJJ_RS08395 at 1545.511 kb on + strand, within MMJJ_RS08395 at 1545.567 kb on + strand, within MMJJ_RS08395 at 1545.576 kb on - strand, within MMJJ_RS08395 at 1545.577 kb on + strand, within MMJJ_RS08395 at 1545.580 kb on - strand, within MMJJ_RS08395 at 1545.580 kb on - strand, within MMJJ_RS08395 at 1545.617 kb on + strand, within MMJJ_RS08395 at 1545.617 kb on + strand, within MMJJ_RS08395 at 1545.621 kb on - strand, within MMJJ_RS08395 at 1545.640 kb on + strand, within MMJJ_RS08395 at 1545.640 kb on + strand, within MMJJ_RS08395 at 1545.640 kb on + strand, within MMJJ_RS08395 at 1545.743 kb on - strand at 1545.772 kb on + strand at 1545.804 kb on + strand at 1545.828 kb on - strand at 1545.839 kb on - strand at 1545.862 kb on - strand at 1545.879 kb on - strand at 1545.915 kb on - strand, within MMJJ_RS08400 at 1546.054 kb on - strand, within MMJJ_RS08400 at 1546.055 kb on + strand, within MMJJ_RS08400 at 1546.091 kb on - strand, within MMJJ_RS08400 at 1546.132 kb on + strand, within MMJJ_RS08400 at 1546.392 kb on - strand at 1546.393 kb on + strand at 1546.402 kb on + strand at 1546.437 kb on - strand at 1546.437 kb on - strand at 1546.438 kb on + strand at 1546.438 kb on + strand at 1546.443 kb on + strand at 1546.464 kb on - strand at 1546.464 kb on - strand at 1546.465 kb on + strand at 1546.466 kb on - strand at 1546.466 kb on - strand at 1546.466 kb on - strand at 1546.466 kb on - strand at 1546.466 kb on - strand at 1546.466 kb on - strand at 1546.466 kb on - strand at 1546.466 kb on - strand at 1546.466 kb on - strand at 1546.466 kb on - strand at 1546.466 kb on - strand at 1546.467 kb on + strand at 1546.467 kb on + strand at 1546.467 kb on + strand at 1546.467 kb on + strand at 1546.467 kb on + strand at 1546.467 kb on + strand at 1546.467 kb on + strand at 1546.467 kb on + strand at 1546.467 kb on + strand at 1546.467 kb on + strand at 1546.468 kb on - strand at 1546.468 kb on - strand at 1546.468 kb on - strand at 1546.468 kb on - strand at 1546.468 kb on - strand at 1546.468 kb on - strand at 1546.469 kb on + strand at 1546.469 kb on + strand at 1546.469 kb on + strand at 1546.469 kb on + strand at 1546.469 kb on + strand at 1546.469 kb on + strand at 1546.469 kb on + strand at 1546.469 kb on + strand at 1546.469 kb on + strand at 1546.469 kb on + strand at 1546.485 kb on - strand at 1546.487 kb on - strand at 1546.488 kb on + strand at 1546.489 kb on - strand at 1546.489 kb on - strand at 1546.490 kb on + strand at 1546.490 kb on + strand at 1546.490 kb on + strand at 1546.490 kb on + strand at 1546.492 kb on - strand at 1546.493 kb on + strand at 1546.495 kb on + strand at 1546.495 kb on + strand at 1546.495 kb on + strand at 1546.502 kb on - strand at 1546.520 kb on - strand at 1546.520 kb on - strand at 1546.520 kb on - strand at 1546.521 kb on + strand at 1546.521 kb on + strand at 1546.528 kb on + strand at 1546.530 kb on - strand at 1546.531 kb on + strand at 1546.533 kb on - strand at 1546.533 kb on - strand at 1546.533 kb on - strand at 1546.533 kb on - strand at 1546.534 kb on + strand at 1546.535 kb on - strand at 1546.535 kb on - strand at 1546.535 kb on - strand at 1546.535 kb on - strand at 1546.535 kb on - strand at 1546.535 kb on - strand at 1546.535 kb on - strand at 1546.535 kb on - strand at 1546.536 kb on + strand at 1546.536 kb on + strand at 1546.536 kb on + strand at 1546.536 kb on + strand at 1546.536 kb on + strand at 1546.536 kb on + strand at 1546.536 kb on + strand at 1546.536 kb on + strand at 1546.536 kb on + strand at 1546.539 kb on - strand at 1546.539 kb on - strand at 1546.539 kb on - strand at 1546.539 kb on - strand at 1546.539 kb on - strand at 1546.539 kb on - strand at 1546.539 kb on - strand at 1546.539 kb on - strand at 1546.539 kb on - strand at 1546.549 kb on - strand at 1546.549 kb on - strand at 1546.549 kb on - strand at 1546.549 kb on - strand at 1546.550 kb on + strand at 1546.550 kb on + strand at 1546.550 kb on + strand at 1546.550 kb on + strand at 1546.556 kb on - strand at 1546.556 kb on - strand at 1546.566 kb on + strand at 1546.616 kb on - strand at 1546.617 kb on + strand at 1546.617 kb on + strand at 1546.647 kb on - strand at 1546.683 kb on + strand at 1546.716 kb on + strand at 1546.729 kb on - strand at 1546.730 kb on + strand at 1546.732 kb on - strand at 1546.732 kb on - strand at 1546.733 kb on + strand at 1546.748 kb on - strand at 1546.760 kb on + strand, within MMJJ_RS08405 at 1546.814 kb on - strand, within MMJJ_RS08405 at 1546.814 kb on - strand, within MMJJ_RS08405 at 1546.820 kb on + strand, within MMJJ_RS08405
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE remove 1,543,920 - -0.6 1,543,921 + +0.6 1,543,921 + +0.7 1,543,936 + -2.0 1,543,990 - MMJJ_RS08380 0.28 -2.4 1,544,018 + +0.1 1,544,018 + MMJJ_RS08380 0.39 -1.9 1,544,018 + MMJJ_RS08380 0.39 -1.8 1,544,090 + MMJJ_RS08380 0.66 +0.7 1,544,313 + MMJJ_RS08385 0.31 -3.4 1,544,317 + MMJJ_RS08385 0.32 +2.9 1,544,320 + MMJJ_RS08385 0.33 +0.8 1,544,347 + MMJJ_RS08385 0.41 -1.5 1,544,358 - MMJJ_RS08385 0.44 +1.4 1,544,376 - MMJJ_RS08385 0.49 -1.1 1,544,377 + MMJJ_RS08385 0.49 -1.7 1,544,382 - MMJJ_RS08385 0.50 +0.7 1,544,382 - MMJJ_RS08385 0.50 +1.5 1,544,382 - MMJJ_RS08385 0.50 -1.8 1,544,383 + MMJJ_RS08385 0.51 -1.9 1,544,391 + MMJJ_RS08385 0.53 -2.4 1,544,428 + MMJJ_RS08385 0.63 +0.8 1,544,448 - MMJJ_RS08385 0.69 -0.4 1,544,449 + MMJJ_RS08385 0.69 -1.3 1,544,491 + MMJJ_RS08385 0.81 -2.1 1,544,547 - +1.4 1,544,559 - -2.2 1,544,559 - +1.1 1,544,560 + +1.2 1,544,560 + +1.6 1,544,587 - +1.3 1,544,587 - +1.5 1,544,588 + -3.1 1,544,604 - MMJJ_RS08390 0.10 +0.1 1,544,627 + MMJJ_RS08390 0.17 -1.5 1,544,629 - MMJJ_RS08390 0.18 -2.9 1,544,633 + MMJJ_RS08390 0.19 +0.6 1,544,790 + MMJJ_RS08390 0.69 +1.1 1,544,823 + MMJJ_RS08390 0.80 -1.1 1,544,916 - -1.9 1,544,921 - +1.7 1,544,921 - +0.9 1,544,922 + -2.1 1,544,922 + +1.2 1,544,922 + +1.1 1,544,928 - -1.1 1,544,932 - +1.9 1,544,932 - -3.0 1,545,003 - -0.2 1,545,032 - MMJJ_RS08395 0.12 +1.1 1,545,121 - MMJJ_RS08395 0.22 +0.9 1,545,212 + MMJJ_RS08395 0.32 -2.2 1,545,237 - MMJJ_RS08395 0.35 -2.9 1,545,238 + MMJJ_RS08395 0.35 +1.6 1,545,249 - MMJJ_RS08395 0.36 -3.6 1,545,250 + MMJJ_RS08395 0.36 -1.6 1,545,251 - MMJJ_RS08395 0.37 -0.7 1,545,251 - MMJJ_RS08395 0.37 -0.3 1,545,251 - MMJJ_RS08395 0.37 +0.6 1,545,251 - MMJJ_RS08395 0.37 +1.7 1,545,251 - MMJJ_RS08395 0.37 +1.7 1,545,251 - MMJJ_RS08395 0.37 +0.7 1,545,252 + MMJJ_RS08395 0.37 -0.6 1,545,252 + MMJJ_RS08395 0.37 +0.8 1,545,252 + MMJJ_RS08395 0.37 -0.8 1,545,252 + MMJJ_RS08395 0.37 +1.6 1,545,254 - MMJJ_RS08395 0.37 +0.1 1,545,254 - MMJJ_RS08395 0.37 -2.0 1,545,254 - MMJJ_RS08395 0.37 +1.3 1,545,254 - MMJJ_RS08395 0.37 -1.6 1,545,254 - MMJJ_RS08395 0.37 +1.4 1,545,255 + MMJJ_RS08395 0.37 +1.3 1,545,255 + MMJJ_RS08395 0.37 -2.2 1,545,257 + MMJJ_RS08395 0.37 -1.3 1,545,321 - MMJJ_RS08395 0.44 -1.1 1,545,322 + MMJJ_RS08395 0.44 +1.3 1,545,329 + MMJJ_RS08395 0.45 -1.5 1,545,337 + MMJJ_RS08395 0.46 -3.4 1,545,337 + MMJJ_RS08395 0.46 +0.5 1,545,343 - MMJJ_RS08395 0.47 +0.6 1,545,343 - MMJJ_RS08395 0.47 -1.5 1,545,344 + MMJJ_RS08395 0.47 -2.4 1,545,344 + MMJJ_RS08395 0.47 -1.5 1,545,347 - MMJJ_RS08395 0.47 +1.0 1,545,359 - MMJJ_RS08395 0.48 -1.8 1,545,431 - MMJJ_RS08395 0.56 +0.9 1,545,432 + MMJJ_RS08395 0.57 +1.3 1,545,436 - MMJJ_RS08395 0.57 -0.9 1,545,436 - MMJJ_RS08395 0.57 -0.7 1,545,436 - MMJJ_RS08395 0.57 +1.3 1,545,472 + MMJJ_RS08395 0.61 +1.2 1,545,473 - MMJJ_RS08395 0.61 +0.4 1,545,473 - MMJJ_RS08395 0.61 +1.1 1,545,473 - MMJJ_RS08395 0.61 +0.9 1,545,473 - MMJJ_RS08395 0.61 +0.7 1,545,505 - MMJJ_RS08395 0.65 +1.0 1,545,505 - MMJJ_RS08395 0.65 +1.7 1,545,505 - MMJJ_RS08395 0.65 +0.7 1,545,510 - MMJJ_RS08395 0.65 +1.5 1,545,510 - MMJJ_RS08395 0.65 -0.5 1,545,510 - MMJJ_RS08395 0.65 +0.5 1,545,511 + MMJJ_RS08395 0.65 +1.5 1,545,567 + MMJJ_RS08395 0.71 +1.4 1,545,576 - MMJJ_RS08395 0.72 +1.8 1,545,577 + MMJJ_RS08395 0.73 -1.1 1,545,580 - MMJJ_RS08395 0.73 +0.8 1,545,580 - MMJJ_RS08395 0.73 -3.5 1,545,617 + MMJJ_RS08395 0.77 +1.3 1,545,617 + MMJJ_RS08395 0.77 +0.5 1,545,621 - MMJJ_RS08395 0.77 +1.3 1,545,640 + MMJJ_RS08395 0.79 -4.4 1,545,640 + MMJJ_RS08395 0.79 +0.0 1,545,640 + MMJJ_RS08395 0.79 +1.4 1,545,743 - +1.4 1,545,772 + +1.1 1,545,804 + -1.4 1,545,828 - +1.7 1,545,839 - +1.1 1,545,862 - +1.3 1,545,879 - -2.1 1,545,915 - MMJJ_RS08400 0.13 -0.9 1,546,054 - MMJJ_RS08400 0.37 -2.4 1,546,055 + MMJJ_RS08400 0.37 +0.5 1,546,091 - MMJJ_RS08400 0.44 +1.3 1,546,132 + MMJJ_RS08400 0.51 -0.6 1,546,392 - +1.1 1,546,393 + +1.9 1,546,402 + -1.8 1,546,437 - +0.9 1,546,437 - +0.2 1,546,438 + +4.0 1,546,438 + +1.5 1,546,443 + -1.8 1,546,464 - +1.7 1,546,464 - +1.7 1,546,465 + +1.2 1,546,466 - +1.2 1,546,466 - -1.5 1,546,466 - +1.3 1,546,466 - +0.7 1,546,466 - -0.2 1,546,466 - -0.9 1,546,466 - +1.0 1,546,466 - -1.5 1,546,466 - +0.1 1,546,466 - -1.2 1,546,466 - -2.5 1,546,467 + -1.5 1,546,467 + -0.3 1,546,467 + +1.1 1,546,467 + +0.2 1,546,467 + -2.6 1,546,467 + -1.3 1,546,467 + -0.5 1,546,467 + +1.0 1,546,467 + +1.9 1,546,467 + -2.4 1,546,468 - +2.5 1,546,468 - +1.0 1,546,468 - -1.6 1,546,468 - +0.8 1,546,468 - +0.7 1,546,468 - -2.0 1,546,469 + -2.8 1,546,469 + +0.7 1,546,469 + -1.7 1,546,469 + +1.3 1,546,469 + +0.9 1,546,469 + +1.6 1,546,469 + +0.8 1,546,469 + +1.5 1,546,469 + +2.3 1,546,469 + +1.2 1,546,485 - -2.9 1,546,487 - -0.9 1,546,488 + +1.7 1,546,489 - +1.7 1,546,489 - -1.7 1,546,490 + -1.6 1,546,490 + -0.1 1,546,490 + +1.9 1,546,490 + -2.3 1,546,492 - +0.9 1,546,493 + +0.9 1,546,495 + +1.2 1,546,495 + +1.7 1,546,495 + +3.0 1,546,502 - +0.1 1,546,520 - +0.5 1,546,520 - -0.1 1,546,520 - -0.8 1,546,521 + -1.6 1,546,521 + -2.7 1,546,528 + -1.9 1,546,530 - -2.4 1,546,531 + +0.1 1,546,533 - -0.7 1,546,533 - -1.9 1,546,533 - -0.4 1,546,533 - -3.2 1,546,534 + -1.9 1,546,535 - -2.8 1,546,535 - -2.1 1,546,535 - -1.5 1,546,535 - -2.5 1,546,535 - -1.0 1,546,535 - -1.5 1,546,535 - -4.1 1,546,535 - -2.9 1,546,536 + +0.1 1,546,536 + -3.7 1,546,536 + -1.3 1,546,536 + -2.4 1,546,536 + -1.9 1,546,536 + -1.5 1,546,536 + +0.1 1,546,536 + -0.9 1,546,536 + -0.8 1,546,539 - -2.7 1,546,539 - -2.8 1,546,539 - -0.6 1,546,539 - -3.6 1,546,539 - -1.3 1,546,539 - +1.0 1,546,539 - -3.2 1,546,539 - -0.9 1,546,539 - -1.4 1,546,549 - +0.1 1,546,549 - -2.6 1,546,549 - -2.4 1,546,549 - -1.9 1,546,550 + -2.1 1,546,550 + -0.9 1,546,550 + -0.9 1,546,550 + -2.1 1,546,556 - -2.7 1,546,556 - -4.0 1,546,566 + -2.5 1,546,616 - -0.9 1,546,617 + -0.9 1,546,617 + +0.1 1,546,647 - -3.8 1,546,683 + -2.5 1,546,716 + -3.6 1,546,729 - -2.2 1,546,730 + -2.4 1,546,732 - -4.2 1,546,732 - -4.4 1,546,733 + -2.6 1,546,748 - -1.9 1,546,760 + MMJJ_RS08405 0.10 -2.4 1,546,814 - MMJJ_RS08405 0.15 -1.5 1,546,814 - MMJJ_RS08405 0.15 -3.6 1,546,820 + MMJJ_RS08405 0.16 -2.2
Or see this region's nucleotide sequence