Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS07830 and MMJJ_RS07835 are separated by 138 nucleotides MMJJ_RS07835 and MMJJ_RS07840 are separated by 96 nucleotides MMJJ_RS07840 and MMJJ_RS07845 are separated by 48 nucleotides
MMJJ_RS07830: MMJJ_RS07830 - DUF1786 domain-containing protein, at 1,434,467 to 1,435,483
_RS07830
MMJJ_RS07835: MMJJ_RS07835 - UPF0489 family protein, at 1,435,622 to 1,436,332
_RS07835
MMJJ_RS07840: MMJJ_RS07840 - Glu-tRNA(Gln) amidotransferase subunit GatD, at 1,436,429 to 1,437,685
_RS07840
MMJJ_RS07845: MMJJ_RS07845 - Glu-tRNA(Gln) amidotransferase subunit GatE, at 1,437,734 to 1,439,629
_RS07845
Position (kb)
1436
1437
1438 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1435.487 kb on + strand at 1435.536 kb on + strand at 1435.541 kb on - strand at 1435.541 kb on - strand at 1435.549 kb on - strand at 1435.550 kb on + strand at 1435.552 kb on + strand at 1435.570 kb on - strand at 1435.570 kb on - strand at 1435.570 kb on - strand at 1435.570 kb on - strand at 1435.571 kb on + strand at 1435.571 kb on + strand at 1435.571 kb on + strand at 1435.571 kb on + strand at 1435.571 kb on + strand at 1435.573 kb on + strand at 1435.584 kb on - strand at 1435.585 kb on + strand at 1435.603 kb on + strand at 1435.621 kb on - strand at 1435.621 kb on - strand at 1435.657 kb on + strand at 1435.820 kb on - strand, within MMJJ_RS07835 at 1435.919 kb on - strand at 1435.925 kb on - strand, within MMJJ_RS07835 at 1435.925 kb on - strand, within MMJJ_RS07835 at 1435.925 kb on - strand, within MMJJ_RS07835 at 1435.925 kb on - strand, within MMJJ_RS07835 at 1435.925 kb on - strand, within MMJJ_RS07835 at 1435.926 kb on + strand, within MMJJ_RS07835 at 1435.926 kb on + strand, within MMJJ_RS07835 at 1435.926 kb on + strand, within MMJJ_RS07835 at 1435.926 kb on + strand, within MMJJ_RS07835 at 1435.926 kb on + strand, within MMJJ_RS07835 at 1435.928 kb on + strand, within MMJJ_RS07835 at 1435.985 kb on - strand, within MMJJ_RS07835 at 1435.997 kb on - strand, within MMJJ_RS07835 at 1436.033 kb on + strand, within MMJJ_RS07835 at 1436.038 kb on + strand, within MMJJ_RS07835 at 1436.042 kb on - strand, within MMJJ_RS07835 at 1436.042 kb on - strand, within MMJJ_RS07835 at 1436.043 kb on + strand, within MMJJ_RS07835 at 1436.047 kb on - strand, within MMJJ_RS07835 at 1436.048 kb on + strand, within MMJJ_RS07835 at 1436.074 kb on + strand, within MMJJ_RS07835 at 1436.250 kb on - strand, within MMJJ_RS07835 at 1436.260 kb on + strand, within MMJJ_RS07835 at 1436.270 kb on - strand at 1436.287 kb on + strand at 1436.290 kb on - strand at 1436.291 kb on + strand at 1436.291 kb on + strand at 1436.291 kb on + strand at 1436.291 kb on + strand at 1436.296 kb on + strand at 1436.316 kb on + strand at 1436.317 kb on - strand at 1436.318 kb on + strand at 1436.318 kb on + strand at 1436.331 kb on + strand at 1436.331 kb on + strand at 1436.334 kb on - strand at 1436.371 kb on + strand at 1436.384 kb on + strand at 1436.384 kb on + strand at 1436.384 kb on + strand at 1436.949 kb on - strand, within MMJJ_RS07840 at 1436.973 kb on - strand, within MMJJ_RS07840 at 1437.105 kb on - strand, within MMJJ_RS07840 at 1437.106 kb on + strand, within MMJJ_RS07840 at 1437.277 kb on + strand, within MMJJ_RS07840
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE remove 1,435,487 + +1.7 1,435,536 + +0.5 1,435,541 - +1.7 1,435,541 - +1.6 1,435,549 - -0.1 1,435,550 + -0.2 1,435,552 + +0.5 1,435,570 - +0.6 1,435,570 - +1.5 1,435,570 - +1.8 1,435,570 - +1.6 1,435,571 + +0.3 1,435,571 + +1.2 1,435,571 + +1.6 1,435,571 + +2.8 1,435,571 + -2.4 1,435,573 + -1.4 1,435,584 - -1.7 1,435,585 + +1.2 1,435,603 + -2.4 1,435,621 - -2.0 1,435,621 - -0.1 1,435,657 + -0.2 1,435,820 - MMJJ_RS07835 0.28 -1.2 1,435,919 - -0.8 1,435,925 - MMJJ_RS07835 0.43 +0.7 1,435,925 - MMJJ_RS07835 0.43 -1.1 1,435,925 - MMJJ_RS07835 0.43 -2.9 1,435,925 - MMJJ_RS07835 0.43 +0.9 1,435,925 - MMJJ_RS07835 0.43 -2.1 1,435,926 + MMJJ_RS07835 0.43 +0.8 1,435,926 + MMJJ_RS07835 0.43 -0.9 1,435,926 + MMJJ_RS07835 0.43 +1.4 1,435,926 + MMJJ_RS07835 0.43 -1.4 1,435,926 + MMJJ_RS07835 0.43 +1.1 1,435,928 + MMJJ_RS07835 0.43 +1.6 1,435,985 - MMJJ_RS07835 0.51 -2.6 1,435,997 - MMJJ_RS07835 0.53 +1.1 1,436,033 + MMJJ_RS07835 0.58 +1.7 1,436,038 + MMJJ_RS07835 0.59 -1.4 1,436,042 - MMJJ_RS07835 0.59 -0.9 1,436,042 - MMJJ_RS07835 0.59 +1.8 1,436,043 + MMJJ_RS07835 0.59 -1.7 1,436,047 - MMJJ_RS07835 0.60 +1.5 1,436,048 + MMJJ_RS07835 0.60 +1.0 1,436,074 + MMJJ_RS07835 0.64 -0.8 1,436,250 - MMJJ_RS07835 0.88 +0.5 1,436,260 + MMJJ_RS07835 0.90 +0.4 1,436,270 - +1.5 1,436,287 + -0.1 1,436,290 - +1.5 1,436,291 + +0.4 1,436,291 + +1.8 1,436,291 + +1.6 1,436,291 + +2.2 1,436,296 + +0.2 1,436,316 + -0.5 1,436,317 - +1.5 1,436,318 + +1.0 1,436,318 + +1.9 1,436,331 + +0.9 1,436,331 + -2.1 1,436,334 - -1.3 1,436,371 + -1.6 1,436,384 + +1.4 1,436,384 + -0.6 1,436,384 + -0.5 1,436,949 - MMJJ_RS07840 0.41 -1.4 1,436,973 - MMJJ_RS07840 0.43 -1.4 1,437,105 - MMJJ_RS07840 0.54 -2.4 1,437,106 + MMJJ_RS07840 0.54 -1.3 1,437,277 + MMJJ_RS07840 0.67 -1.8
Or see this region's nucleotide sequence