Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS06950 and MMJJ_RS06955 are separated by 1 nucleotides MMJJ_RS06955 and MMJJ_RS06960 are separated by 151 nucleotides
MMJJ_RS06950: MMJJ_RS06950 - 4-demethylwyosine synthase TYW1, at 1,275,141 to 1,276,082
_RS06950
MMJJ_RS06955: MMJJ_RS06955 - 2,3-bisphosphoglycerate-independent phosphoglycerate mutase, at 1,276,084 to 1,277,304
_RS06955
MMJJ_RS06960: MMJJ_RS06960 - CAP domain-containing protein, at 1,277,456 to 1,278,904
_RS06960
Position (kb) 1276
1277
1278 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 1275.086 kb on - strand at 1275.086 kb on - strand at 1275.087 kb on + strand at 1275.092 kb on + strand at 1275.100 kb on - strand at 1275.100 kb on - strand at 1275.101 kb on + strand at 1275.105 kb on + strand at 1275.105 kb on + strand at 1275.108 kb on + strand at 1275.108 kb on + strand at 1275.112 kb on + strand at 1275.126 kb on - strand at 1275.126 kb on - strand at 1275.126 kb on - strand at 1275.127 kb on + strand at 1275.128 kb on - strand at 1275.128 kb on - strand at 1275.128 kb on - strand at 1275.136 kb on - strand at 1275.136 kb on - strand at 1275.137 kb on + strand at 1275.137 kb on + strand at 1275.141 kb on + strand at 1275.141 kb on + strand at 1275.157 kb on - strand at 1275.158 kb on + strand at 1275.164 kb on - strand at 1275.165 kb on + strand at 1275.191 kb on + strand at 1275.371 kb on + strand, within MMJJ_RS06950 at 1275.452 kb on - strand, within MMJJ_RS06950 at 1275.453 kb on + strand, within MMJJ_RS06950 at 1275.462 kb on - strand, within MMJJ_RS06950 at 1275.463 kb on + strand, within MMJJ_RS06950 at 1275.498 kb on - strand, within MMJJ_RS06950 at 1275.532 kb on - strand, within MMJJ_RS06950 at 1275.533 kb on + strand, within MMJJ_RS06950 at 1275.533 kb on + strand, within MMJJ_RS06950 at 1275.551 kb on - strand, within MMJJ_RS06950 at 1275.551 kb on - strand, within MMJJ_RS06950 at 1275.552 kb on + strand, within MMJJ_RS06950 at 1275.552 kb on + strand, within MMJJ_RS06950 at 1275.552 kb on + strand, within MMJJ_RS06950 at 1275.558 kb on - strand, within MMJJ_RS06950 at 1275.558 kb on - strand, within MMJJ_RS06950 at 1275.564 kb on - strand, within MMJJ_RS06950 at 1275.565 kb on + strand, within MMJJ_RS06950 at 1275.565 kb on + strand, within MMJJ_RS06950 at 1275.577 kb on - strand, within MMJJ_RS06950 at 1275.634 kb on - strand, within MMJJ_RS06950 at 1275.643 kb on - strand, within MMJJ_RS06950 at 1275.644 kb on + strand, within MMJJ_RS06950 at 1275.644 kb on + strand, within MMJJ_RS06950 at 1275.644 kb on + strand, within MMJJ_RS06950 at 1275.644 kb on + strand, within MMJJ_RS06950 at 1275.644 kb on + strand, within MMJJ_RS06950 at 1275.655 kb on - strand, within MMJJ_RS06950 at 1275.673 kb on + strand, within MMJJ_RS06950 at 1275.675 kb on + strand, within MMJJ_RS06950 at 1275.746 kb on - strand, within MMJJ_RS06950 at 1275.937 kb on - strand, within MMJJ_RS06950 at 1275.937 kb on - strand, within MMJJ_RS06950 at 1275.938 kb on + strand, within MMJJ_RS06950 at 1275.988 kb on - strand at 1275.988 kb on - strand at 1275.988 kb on - strand at 1275.988 kb on - strand at 1275.989 kb on + strand at 1275.989 kb on + strand at 1275.989 kb on + strand at 1275.989 kb on + strand at 1276.030 kb on - strand at 1276.031 kb on + strand at 1276.031 kb on + strand at 1276.031 kb on + strand at 1276.035 kb on + strand at 1276.040 kb on - strand at 1276.086 kb on + strand at 1276.095 kb on + strand at 1276.173 kb on + strand at 1276.707 kb on - strand, within MMJJ_RS06955 at 1276.881 kb on - strand, within MMJJ_RS06955 at 1277.374 kb on - strand at 1277.404 kb on - strand at 1277.405 kb on + strand at 1277.441 kb on + strand at 1277.448 kb on - strand at 1277.448 kb on - strand at 1277.454 kb on + strand at 1277.457 kb on - strand at 1277.458 kb on + strand at 1277.458 kb on + strand at 1277.458 kb on + strand at 1277.480 kb on + strand at 1277.481 kb on - strand at 1277.481 kb on - strand at 1277.481 kb on - strand at 1277.482 kb on + strand at 1277.482 kb on + strand at 1277.484 kb on - strand at 1277.484 kb on - strand at 1277.484 kb on - strand at 1277.484 kb on - strand at 1277.484 kb on - strand at 1277.485 kb on + strand at 1277.485 kb on + strand at 1277.485 kb on + strand at 1277.485 kb on + strand at 1277.485 kb on + strand at 1277.485 kb on + strand at 1277.488 kb on - strand at 1277.489 kb on + strand at 1277.489 kb on + strand at 1277.489 kb on + strand at 1277.509 kb on - strand at 1277.513 kb on - strand at 1277.513 kb on - strand at 1277.513 kb on - strand at 1277.513 kb on - strand at 1277.513 kb on - strand at 1277.513 kb on - strand at 1277.513 kb on - strand at 1277.513 kb on - strand at 1277.514 kb on + strand at 1277.514 kb on + strand at 1277.514 kb on + strand at 1277.514 kb on + strand at 1277.514 kb on + strand at 1277.514 kb on + strand at 1277.514 kb on + strand at 1277.514 kb on + strand at 1277.534 kb on - strand at 1277.535 kb on + strand at 1277.594 kb on + strand at 1277.594 kb on + strand at 1277.616 kb on - strand, within MMJJ_RS06960 at 1277.617 kb on + strand, within MMJJ_RS06960 at 1277.617 kb on + strand, within MMJJ_RS06960 at 1277.631 kb on + strand, within MMJJ_RS06960 at 1277.631 kb on + strand, within MMJJ_RS06960 at 1277.679 kb on - strand, within MMJJ_RS06960 at 1277.698 kb on + strand, within MMJJ_RS06960 at 1277.702 kb on - strand, within MMJJ_RS06960 at 1277.703 kb on + strand, within MMJJ_RS06960 at 1277.713 kb on + strand, within MMJJ_RS06960 at 1277.760 kb on + strand, within MMJJ_RS06960 at 1277.772 kb on - strand, within MMJJ_RS06960 at 1277.828 kb on + strand, within MMJJ_RS06960 at 1277.834 kb on - strand, within MMJJ_RS06960 at 1277.835 kb on + strand, within MMJJ_RS06960 at 1277.853 kb on - strand, within MMJJ_RS06960 at 1277.872 kb on - strand, within MMJJ_RS06960 at 1277.872 kb on - strand, within MMJJ_RS06960 at 1277.872 kb on - strand, within MMJJ_RS06960 at 1277.872 kb on - strand, within MMJJ_RS06960 at 1277.873 kb on + strand, within MMJJ_RS06960 at 1277.873 kb on + strand, within MMJJ_RS06960 at 1277.873 kb on + strand, within MMJJ_RS06960 at 1277.875 kb on - strand, within MMJJ_RS06960 at 1277.875 kb on - strand, within MMJJ_RS06960 at 1277.875 kb on - strand, within MMJJ_RS06960 at 1277.875 kb on - strand, within MMJJ_RS06960 at 1277.876 kb on + strand, within MMJJ_RS06960 at 1277.876 kb on + strand, within MMJJ_RS06960 at 1277.876 kb on + strand, within MMJJ_RS06960 at 1277.876 kb on + strand, within MMJJ_RS06960 at 1277.877 kb on - strand, within MMJJ_RS06960 at 1277.877 kb on - strand, within MMJJ_RS06960 at 1277.877 kb on - strand, within MMJJ_RS06960 at 1277.878 kb on + strand, within MMJJ_RS06960 at 1277.878 kb on + strand, within MMJJ_RS06960 at 1277.885 kb on - strand, within MMJJ_RS06960 at 1277.885 kb on - strand, within MMJJ_RS06960 at 1277.886 kb on + strand, within MMJJ_RS06960 at 1277.886 kb on + strand, within MMJJ_RS06960 at 1277.886 kb on + strand, within MMJJ_RS06960 at 1277.886 kb on + strand, within MMJJ_RS06960 at 1277.887 kb on - strand, within MMJJ_RS06960 at 1277.887 kb on - strand, within MMJJ_RS06960 at 1277.887 kb on - strand, within MMJJ_RS06960 at 1277.887 kb on - strand, within MMJJ_RS06960 at 1277.887 kb on - strand, within MMJJ_RS06960 at 1277.888 kb on + strand, within MMJJ_RS06960 at 1277.888 kb on + strand, within MMJJ_RS06960 at 1277.888 kb on + strand, within MMJJ_RS06960 at 1277.888 kb on + strand, within MMJJ_RS06960 at 1277.888 kb on + strand, within MMJJ_RS06960 at 1277.888 kb on + strand, within MMJJ_RS06960 at 1277.888 kb on + strand, within MMJJ_RS06960 at 1277.888 kb on + strand, within MMJJ_RS06960 at 1277.888 kb on + strand, within MMJJ_RS06960 at 1277.888 kb on + strand, within MMJJ_RS06960 at 1277.888 kb on + strand, within MMJJ_RS06960 at 1277.889 kb on - strand, within MMJJ_RS06960 at 1277.889 kb on - strand, within MMJJ_RS06960 at 1277.890 kb on + strand, within MMJJ_RS06960 at 1277.890 kb on + strand, within MMJJ_RS06960 at 1277.890 kb on + strand, within MMJJ_RS06960 at 1277.890 kb on + strand, within MMJJ_RS06960 at 1277.911 kb on - strand, within MMJJ_RS06960 at 1277.915 kb on - strand, within MMJJ_RS06960 at 1277.916 kb on + strand, within MMJJ_RS06960 at 1277.916 kb on + strand, within MMJJ_RS06960 at 1277.916 kb on + strand, within MMJJ_RS06960 at 1277.916 kb on + strand, within MMJJ_RS06960 at 1277.916 kb on + strand, within MMJJ_RS06960 at 1277.916 kb on + strand, within MMJJ_RS06960 at 1277.916 kb on + strand, within MMJJ_RS06960 at 1277.916 kb on + strand, within MMJJ_RS06960 at 1277.916 kb on + strand, within MMJJ_RS06960 at 1277.918 kb on - strand, within MMJJ_RS06960 at 1277.919 kb on + strand, within MMJJ_RS06960 at 1277.919 kb on + strand, within MMJJ_RS06960 at 1277.930 kb on - strand, within MMJJ_RS06960 at 1277.933 kb on - strand, within MMJJ_RS06960 at 1277.945 kb on - strand, within MMJJ_RS06960 at 1277.963 kb on - strand, within MMJJ_RS06960 at 1277.963 kb on - strand, within MMJJ_RS06960 at 1277.978 kb on - strand, within MMJJ_RS06960 at 1278.156 kb on + strand, within MMJJ_RS06960 at 1278.171 kb on + strand, within MMJJ_RS06960 at 1278.193 kb on - strand, within MMJJ_RS06960 at 1278.199 kb on - strand, within MMJJ_RS06960 at 1278.199 kb on - strand, within MMJJ_RS06960 at 1278.199 kb on - strand, within MMJJ_RS06960 at 1278.199 kb on - strand, within MMJJ_RS06960 at 1278.200 kb on + strand, within MMJJ_RS06960 at 1278.202 kb on + strand, within MMJJ_RS06960 at 1278.203 kb on - strand, within MMJJ_RS06960 at 1278.249 kb on - strand, within MMJJ_RS06960 at 1278.274 kb on - strand, within MMJJ_RS06960
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE remove 1,275,086 - -2.3 1,275,086 - +0.6 1,275,087 + -0.5 1,275,092 + -0.4 1,275,100 - +1.2 1,275,100 - +0.9 1,275,101 + +1.1 1,275,105 + -1.5 1,275,105 + -3.6 1,275,108 + -0.9 1,275,108 + -1.8 1,275,112 + -2.5 1,275,126 - +2.4 1,275,126 - -2.1 1,275,126 - -0.4 1,275,127 + -1.2 1,275,128 - -1.6 1,275,128 - +1.2 1,275,128 - -1.4 1,275,136 - -1.1 1,275,136 - -0.3 1,275,137 + +0.4 1,275,137 + -0.5 1,275,141 + +2.0 1,275,141 + +0.9 1,275,157 - +0.7 1,275,158 + -1.0 1,275,164 - +2.2 1,275,165 + -1.1 1,275,191 + -0.6 1,275,371 + MMJJ_RS06950 0.24 -0.2 1,275,452 - MMJJ_RS06950 0.33 +2.0 1,275,453 + MMJJ_RS06950 0.33 +2.8 1,275,462 - MMJJ_RS06950 0.34 +2.3 1,275,463 + MMJJ_RS06950 0.34 +1.8 1,275,498 - MMJJ_RS06950 0.38 +3.5 1,275,532 - MMJJ_RS06950 0.42 +3.2 1,275,533 + MMJJ_RS06950 0.42 -0.7 1,275,533 + MMJJ_RS06950 0.42 -0.0 1,275,551 - MMJJ_RS06950 0.44 +2.5 1,275,551 - MMJJ_RS06950 0.44 +1.8 1,275,552 + MMJJ_RS06950 0.44 +2.3 1,275,552 + MMJJ_RS06950 0.44 -0.6 1,275,552 + MMJJ_RS06950 0.44 -1.0 1,275,558 - MMJJ_RS06950 0.44 +1.8 1,275,558 - MMJJ_RS06950 0.44 +2.5 1,275,564 - MMJJ_RS06950 0.45 -0.6 1,275,565 + MMJJ_RS06950 0.45 -0.0 1,275,565 + MMJJ_RS06950 0.45 +2.5 1,275,577 - MMJJ_RS06950 0.46 -0.4 1,275,634 - MMJJ_RS06950 0.52 +0.5 1,275,643 - MMJJ_RS06950 0.53 -0.8 1,275,644 + MMJJ_RS06950 0.53 -2.3 1,275,644 + MMJJ_RS06950 0.53 +2.2 1,275,644 + MMJJ_RS06950 0.53 -0.4 1,275,644 + MMJJ_RS06950 0.53 +2.4 1,275,644 + MMJJ_RS06950 0.53 +2.3 1,275,655 - MMJJ_RS06950 0.55 -0.2 1,275,673 + MMJJ_RS06950 0.56 -1.4 1,275,675 + MMJJ_RS06950 0.57 +1.4 1,275,746 - MMJJ_RS06950 0.64 +2.7 1,275,937 - MMJJ_RS06950 0.85 +2.0 1,275,937 - MMJJ_RS06950 0.85 +2.3 1,275,938 + MMJJ_RS06950 0.85 +2.9 1,275,988 - +2.5 1,275,988 - +2.9 1,275,988 - +0.8 1,275,988 - +0.9 1,275,989 + -0.2 1,275,989 + +1.9 1,275,989 + +2.1 1,275,989 + +2.9 1,276,030 - -0.4 1,276,031 + +2.3 1,276,031 + -1.1 1,276,031 + +0.3 1,276,035 + +0.6 1,276,040 - -0.1 1,276,086 + -1.6 1,276,095 + +0.7 1,276,173 + -2.4 1,276,707 - MMJJ_RS06955 0.51 +1.9 1,276,881 - MMJJ_RS06955 0.65 +0.4 1,277,374 - +0.4 1,277,404 - -3.4 1,277,405 + +1.4 1,277,441 + +1.5 1,277,448 - +2.5 1,277,448 - +0.4 1,277,454 + +1.0 1,277,457 - -0.4 1,277,458 + -0.7 1,277,458 + -1.2 1,277,458 + +0.9 1,277,480 + +1.9 1,277,481 - +1.5 1,277,481 - +1.5 1,277,481 - -2.6 1,277,482 + -1.9 1,277,482 + +0.4 1,277,484 - +1.2 1,277,484 - -1.2 1,277,484 - -2.6 1,277,484 - -1.2 1,277,484 - -0.2 1,277,485 + +1.2 1,277,485 + -1.5 1,277,485 + -2.6 1,277,485 + -0.2 1,277,485 + -2.6 1,277,485 + -1.3 1,277,488 - -2.2 1,277,489 + +1.6 1,277,489 + +1.1 1,277,489 + +2.0 1,277,509 - -1.4 1,277,513 - +1.9 1,277,513 - +0.1 1,277,513 - +0.6 1,277,513 - +2.0 1,277,513 - -2.2 1,277,513 - -1.0 1,277,513 - +1.6 1,277,513 - +0.4 1,277,514 + -1.7 1,277,514 + -0.7 1,277,514 + -1.9 1,277,514 + +0.4 1,277,514 + -1.7 1,277,514 + -0.1 1,277,514 + -2.3 1,277,514 + -0.7 1,277,534 - +1.0 1,277,535 + +1.5 1,277,594 + +1.0 1,277,594 + +1.6 1,277,616 - MMJJ_RS06960 0.11 +0.9 1,277,617 + MMJJ_RS06960 0.11 -0.8 1,277,617 + MMJJ_RS06960 0.11 +1.7 1,277,631 + MMJJ_RS06960 0.12 +0.1 1,277,631 + MMJJ_RS06960 0.12 +0.6 1,277,679 - MMJJ_RS06960 0.15 +0.9 1,277,698 + MMJJ_RS06960 0.17 -0.3 1,277,702 - MMJJ_RS06960 0.17 -0.4 1,277,703 + MMJJ_RS06960 0.17 +0.2 1,277,713 + MMJJ_RS06960 0.18 +1.4 1,277,760 + MMJJ_RS06960 0.21 +0.5 1,277,772 - MMJJ_RS06960 0.22 -1.5 1,277,828 + MMJJ_RS06960 0.26 -0.6 1,277,834 - MMJJ_RS06960 0.26 +1.6 1,277,835 + MMJJ_RS06960 0.26 -2.1 1,277,853 - MMJJ_RS06960 0.27 +1.3 1,277,872 - MMJJ_RS06960 0.29 +0.6 1,277,872 - MMJJ_RS06960 0.29 +1.6 1,277,872 - MMJJ_RS06960 0.29 +1.7 1,277,872 - MMJJ_RS06960 0.29 -1.4 1,277,873 + MMJJ_RS06960 0.29 +1.8 1,277,873 + MMJJ_RS06960 0.29 -0.2 1,277,873 + MMJJ_RS06960 0.29 -2.3 1,277,875 - MMJJ_RS06960 0.29 -2.3 1,277,875 - MMJJ_RS06960 0.29 +1.1 1,277,875 - MMJJ_RS06960 0.29 -0.1 1,277,875 - MMJJ_RS06960 0.29 +1.5 1,277,876 + MMJJ_RS06960 0.29 -0.6 1,277,876 + MMJJ_RS06960 0.29 +0.9 1,277,876 + MMJJ_RS06960 0.29 -0.4 1,277,876 + MMJJ_RS06960 0.29 +1.2 1,277,877 - MMJJ_RS06960 0.29 +1.1 1,277,877 - MMJJ_RS06960 0.29 +1.4 1,277,877 - MMJJ_RS06960 0.29 -1.8 1,277,878 + MMJJ_RS06960 0.29 -1.8 1,277,878 + MMJJ_RS06960 0.29 +1.1 1,277,885 - MMJJ_RS06960 0.30 +1.0 1,277,885 - MMJJ_RS06960 0.30 -2.5 1,277,886 + MMJJ_RS06960 0.30 +2.2 1,277,886 + MMJJ_RS06960 0.30 +1.6 1,277,886 + MMJJ_RS06960 0.30 +2.1 1,277,886 + MMJJ_RS06960 0.30 +2.2 1,277,887 - MMJJ_RS06960 0.30 +1.0 1,277,887 - MMJJ_RS06960 0.30 +0.6 1,277,887 - MMJJ_RS06960 0.30 -2.0 1,277,887 - MMJJ_RS06960 0.30 -2.3 1,277,887 - MMJJ_RS06960 0.30 -2.3 1,277,888 + MMJJ_RS06960 0.30 -0.1 1,277,888 + MMJJ_RS06960 0.30 +0.3 1,277,888 + MMJJ_RS06960 0.30 +2.0 1,277,888 + MMJJ_RS06960 0.30 +1.3 1,277,888 + MMJJ_RS06960 0.30 -1.2 1,277,888 + MMJJ_RS06960 0.30 +0.9 1,277,888 + MMJJ_RS06960 0.30 +1.4 1,277,888 + MMJJ_RS06960 0.30 -0.9 1,277,888 + MMJJ_RS06960 0.30 +1.4 1,277,888 + MMJJ_RS06960 0.30 +1.1 1,277,888 + MMJJ_RS06960 0.30 +1.8 1,277,889 - MMJJ_RS06960 0.30 -0.1 1,277,889 - MMJJ_RS06960 0.30 -1.5 1,277,890 + MMJJ_RS06960 0.30 -1.5 1,277,890 + MMJJ_RS06960 0.30 -0.1 1,277,890 + MMJJ_RS06960 0.30 +1.4 1,277,890 + MMJJ_RS06960 0.30 -1.3 1,277,911 - MMJJ_RS06960 0.31 -0.6 1,277,915 - MMJJ_RS06960 0.32 -0.6 1,277,916 + MMJJ_RS06960 0.32 -2.8 1,277,916 + MMJJ_RS06960 0.32 +1.8 1,277,916 + MMJJ_RS06960 0.32 +0.1 1,277,916 + MMJJ_RS06960 0.32 +1.9 1,277,916 + MMJJ_RS06960 0.32 -0.1 1,277,916 + MMJJ_RS06960 0.32 -2.5 1,277,916 + MMJJ_RS06960 0.32 -1.5 1,277,916 + MMJJ_RS06960 0.32 -0.8 1,277,916 + MMJJ_RS06960 0.32 +2.0 1,277,918 - MMJJ_RS06960 0.32 +1.3 1,277,919 + MMJJ_RS06960 0.32 -1.1 1,277,919 + MMJJ_RS06960 0.32 +2.1 1,277,930 - MMJJ_RS06960 0.33 -2.2 1,277,933 - MMJJ_RS06960 0.33 -1.2 1,277,945 - MMJJ_RS06960 0.34 -3.1 1,277,963 - MMJJ_RS06960 0.35 +1.4 1,277,963 - MMJJ_RS06960 0.35 -1.3 1,277,978 - MMJJ_RS06960 0.36 -2.0 1,278,156 + MMJJ_RS06960 0.48 +2.0 1,278,171 + MMJJ_RS06960 0.49 +0.9 1,278,193 - MMJJ_RS06960 0.51 -1.0 1,278,199 - MMJJ_RS06960 0.51 +1.5 1,278,199 - MMJJ_RS06960 0.51 -1.8 1,278,199 - MMJJ_RS06960 0.51 -0.4 1,278,199 - MMJJ_RS06960 0.51 -2.6 1,278,200 + MMJJ_RS06960 0.51 -1.2 1,278,202 + MMJJ_RS06960 0.51 -0.6 1,278,203 - MMJJ_RS06960 0.52 -2.0 1,278,249 - MMJJ_RS06960 0.55 -0.2 1,278,274 - MMJJ_RS06960 0.56 -0.6
Or see this region's nucleotide sequence