Strain Fitness in Methanococcus maripaludis JJ around MMJJ_RS04330

Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMMJJ_RS04325 and MMJJ_RS04330 overlap by 20 nucleotidesMMJJ_RS04330 and MMJJ_RS04335 are separated by 1 nucleotides MMJJ_RS04325: MMJJ_RS04325 - tyrosine-type recombinase/integrase, at 807,960 to 808,928 _RS04325 MMJJ_RS04330: MMJJ_RS04330 - DUF2540 domain-containing protein, at 808,909 to 809,186 _RS04330 MMJJ_RS04335: MMJJ_RS04335 - minichromosome maintenance protein MCM, at 809,188 to 811,212 _RS04335 Position (kb) 808 809 810Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 807.984 kb on - strandat 808.018 kb on - strandat 808.140 kb on + strand, within MMJJ_RS04325at 808.200 kb on - strand, within MMJJ_RS04325at 808.274 kb on + strand, within MMJJ_RS04325at 808.281 kb on + strand, within MMJJ_RS04325at 808.290 kb on - strand, within MMJJ_RS04325at 808.347 kb on + strand, within MMJJ_RS04325at 808.352 kb on - strand, within MMJJ_RS04325at 808.374 kb on + strand, within MMJJ_RS04325at 808.374 kb on + strand, within MMJJ_RS04325at 808.450 kb on + strand, within MMJJ_RS04325at 808.462 kb on + strand, within MMJJ_RS04325at 808.462 kb on + strand, within MMJJ_RS04325at 808.462 kb on + strand, within MMJJ_RS04325at 808.490 kb on - strand, within MMJJ_RS04325at 808.491 kb on + strand, within MMJJ_RS04325at 808.500 kb on - strand, within MMJJ_RS04325at 808.500 kb on - strand, within MMJJ_RS04325at 808.500 kb on - strand, within MMJJ_RS04325at 808.524 kb on - strand, within MMJJ_RS04325at 808.525 kb on + strand, within MMJJ_RS04325at 808.526 kb on - strand, within MMJJ_RS04325at 808.527 kb on + strand, within MMJJ_RS04325at 808.542 kb on - strand, within MMJJ_RS04325at 808.620 kb on + strand, within MMJJ_RS04325at 808.690 kb on - strand, within MMJJ_RS04325at 808.704 kb on - strand, within MMJJ_RS04325at 808.902 kb on - strandat 808.911 kb on + strandat 808.962 kb on - strand, within MMJJ_RS04330at 808.962 kb on - strand, within MMJJ_RS04330at 808.963 kb on + strand, within MMJJ_RS04330at 808.966 kb on + strand, within MMJJ_RS04330at 808.969 kb on - strand, within MMJJ_RS04330at 808.969 kb on - strandat 808.970 kb on + strand, within MMJJ_RS04330at 808.970 kb on + strand, within MMJJ_RS04330at 808.970 kb on + strand, within MMJJ_RS04330at 808.974 kb on - strand, within MMJJ_RS04330at 809.094 kb on - strand, within MMJJ_RS04330at 809.094 kb on - strand, within MMJJ_RS04330at 809.094 kb on - strand, within MMJJ_RS04330at 809.094 kb on - strand, within MMJJ_RS04330at 809.094 kb on - strand, within MMJJ_RS04330at 809.094 kb on - strand, within MMJJ_RS04330at 809.094 kb on - strand, within MMJJ_RS04330at 809.095 kb on + strand, within MMJJ_RS04330at 809.095 kb on + strand, within MMJJ_RS04330at 809.095 kb on + strand, within MMJJ_RS04330at 809.095 kb on + strand, within MMJJ_RS04330at 809.095 kb on + strand, within MMJJ_RS04330at 809.095 kb on + strand, within MMJJ_RS04330at 809.095 kb on + strand, within MMJJ_RS04330at 809.095 kb on - strand, within MMJJ_RS04330at 809.098 kb on - strand, within MMJJ_RS04330at 809.098 kb on - strand, within MMJJ_RS04330at 809.098 kb on - strand, within MMJJ_RS04330at 809.098 kb on - strand, within MMJJ_RS04330at 809.099 kb on + strand, within MMJJ_RS04330at 809.099 kb on + strand, within MMJJ_RS04330at 809.125 kb on - strand, within MMJJ_RS04330at 809.126 kb on + strand, within MMJJ_RS04330at 809.126 kb on + strand, within MMJJ_RS04330at 809.126 kb on + strand, within MMJJ_RS04330at 809.126 kb on + strand, within MMJJ_RS04330at 809.126 kb on + strand, within MMJJ_RS04330at 809.129 kb on + strand, within MMJJ_RS04330at 809.159 kb on - strandat 809.160 kb on + strandat 809.167 kb on + strandat 809.167 kb on + strandat 809.261 kb on + strandat 809.267 kb on + strandat 809.287 kb on + strandat 809.330 kb on + strandat 809.362 kb on - strandat 809.362 kb on - strandat 809.364 kb on - strandat 809.367 kb on - strandat 809.373 kb on + strandat 809.384 kb on + strandat 809.468 kb on + strand, within MMJJ_RS04335at 809.486 kb on - strand, within MMJJ_RS04335at 809.486 kb on - strand, within MMJJ_RS04335at 809.487 kb on + strand, within MMJJ_RS04335at 809.487 kb on + strand, within MMJJ_RS04335at 809.518 kb on - strand, within MMJJ_RS04335at 809.608 kb on - strand, within MMJJ_RS04335at 809.619 kb on + strand, within MMJJ_RS04335at 809.632 kb on - strand, within MMJJ_RS04335at 809.640 kb on - strand, within MMJJ_RS04335at 809.679 kb on + strand, within MMJJ_RS04335at 809.679 kb on + strand, within MMJJ_RS04335at 809.688 kb on + strand, within MMJJ_RS04335at 809.717 kb on + strand, within MMJJ_RS04335at 809.774 kb on - strand, within MMJJ_RS04335at 809.797 kb on + strand, within MMJJ_RS04335at 809.873 kb on + strand, within MMJJ_RS04335at 809.940 kb on - strand, within MMJJ_RS04335at 810.004 kb on - strand, within MMJJ_RS04335at 810.005 kb on + strand, within MMJJ_RS04335at 810.005 kb on + strand, within MMJJ_RS04335at 810.005 kb on + strand, within MMJJ_RS04335at 810.033 kb on - strand, within MMJJ_RS04335at 810.035 kb on - strand, within MMJJ_RS04335at 810.035 kb on - strand, within MMJJ_RS04335at 810.035 kb on - strand, within MMJJ_RS04335at 810.036 kb on + strand, within MMJJ_RS04335at 810.097 kb on - strand, within MMJJ_RS04335at 810.098 kb on + strand, within MMJJ_RS04335at 810.103 kb on - strand, within MMJJ_RS04335at 810.104 kb on + strand, within MMJJ_RS04335at 810.115 kb on - strand, within MMJJ_RS04335at 810.115 kb on - strand, within MMJJ_RS04335at 810.115 kb on - strand, within MMJJ_RS04335at 810.116 kb on + strand, within MMJJ_RS04335at 810.126 kb on - strand, within MMJJ_RS04335

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Per-strain Table

Position Strand Gene LocusTag Fraction Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE
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807,984 - -1.0
808,018 - +1.1
808,140 + MMJJ_RS04325 0.19 -2.2
808,200 - MMJJ_RS04325 0.25 -0.6
808,274 + MMJJ_RS04325 0.32 +1.4
808,281 + MMJJ_RS04325 0.33 +2.0
808,290 - MMJJ_RS04325 0.34 -1.4
808,347 + MMJJ_RS04325 0.40 +1.2
808,352 - MMJJ_RS04325 0.40 +0.4
808,374 + MMJJ_RS04325 0.43 +0.6
808,374 + MMJJ_RS04325 0.43 +0.7
808,450 + MMJJ_RS04325 0.51 +1.2
808,462 + MMJJ_RS04325 0.52 -0.6
808,462 + MMJJ_RS04325 0.52 -0.9
808,462 + MMJJ_RS04325 0.52 +1.0
808,490 - MMJJ_RS04325 0.55 -0.2
808,491 + MMJJ_RS04325 0.55 +0.4
808,500 - MMJJ_RS04325 0.56 +1.0
808,500 - MMJJ_RS04325 0.56 -1.7
808,500 - MMJJ_RS04325 0.56 +2.4
808,524 - MMJJ_RS04325 0.58 +1.2
808,525 + MMJJ_RS04325 0.58 +2.2
808,526 - MMJJ_RS04325 0.58 -1.1
808,527 + MMJJ_RS04325 0.59 -1.4
808,542 - MMJJ_RS04325 0.60 -1.5
808,620 + MMJJ_RS04325 0.68 +1.4
808,690 - MMJJ_RS04325 0.75 +0.1
808,704 - MMJJ_RS04325 0.77 -3.1
808,902 - +0.1
808,911 + +0.2
808,962 - MMJJ_RS04330 0.19 -1.5
808,962 - MMJJ_RS04330 0.19 +0.7
808,963 + MMJJ_RS04330 0.19 -2.8
808,966 + MMJJ_RS04330 0.21 -3.0
808,969 - MMJJ_RS04330 0.22 -0.4
808,969 - -1.4
808,970 + MMJJ_RS04330 0.22 +1.4
808,970 + MMJJ_RS04330 0.22 -0.4
808,970 + MMJJ_RS04330 0.22 -2.2
808,974 - MMJJ_RS04330 0.23 +0.2
809,094 - MMJJ_RS04330 0.67 +1.5
809,094 - MMJJ_RS04330 0.67 -1.1
809,094 - MMJJ_RS04330 0.67 +0.2
809,094 - MMJJ_RS04330 0.67 -1.8
809,094 - MMJJ_RS04330 0.67 -1.8
809,094 - MMJJ_RS04330 0.67 +0.3
809,094 - MMJJ_RS04330 0.67 +1.1
809,095 + MMJJ_RS04330 0.67 -1.6
809,095 + MMJJ_RS04330 0.67 +1.7
809,095 + MMJJ_RS04330 0.67 -1.0
809,095 + MMJJ_RS04330 0.67 -1.4
809,095 + MMJJ_RS04330 0.67 +0.9
809,095 + MMJJ_RS04330 0.67 -1.2
809,095 + MMJJ_RS04330 0.67 -2.9
809,095 - MMJJ_RS04330 0.67 +1.3
809,098 - MMJJ_RS04330 0.68 -1.8
809,098 - MMJJ_RS04330 0.68 -1.8
809,098 - MMJJ_RS04330 0.68 +0.4
809,098 - MMJJ_RS04330 0.68 -1.8
809,099 + MMJJ_RS04330 0.68 +1.0
809,099 + MMJJ_RS04330 0.68 +0.8
809,125 - MMJJ_RS04330 0.78 +1.4
809,126 + MMJJ_RS04330 0.78 -1.7
809,126 + MMJJ_RS04330 0.78 -1.2
809,126 + MMJJ_RS04330 0.78 +0.8
809,126 + MMJJ_RS04330 0.78 -1.1
809,126 + MMJJ_RS04330 0.78 +0.9
809,129 + MMJJ_RS04330 0.79 -0.9
809,159 - +0.8
809,160 + +1.2
809,167 + +0.8
809,167 + -0.5
809,261 + -1.3
809,267 + +1.7
809,287 + -0.4
809,330 + -1.8
809,362 - +0.6
809,362 - +1.4
809,364 - +0.6
809,367 - -1.7
809,373 + +2.1
809,384 + -0.5
809,468 + MMJJ_RS04335 0.14 -2.1
809,486 - MMJJ_RS04335 0.15 -3.8
809,486 - MMJJ_RS04335 0.15 +1.5
809,487 + MMJJ_RS04335 0.15 +1.2
809,487 + MMJJ_RS04335 0.15 +1.8
809,518 - MMJJ_RS04335 0.16 +1.0
809,608 - MMJJ_RS04335 0.21 +0.6
809,619 + MMJJ_RS04335 0.21 +0.8
809,632 - MMJJ_RS04335 0.22 -0.8
809,640 - MMJJ_RS04335 0.22 +0.6
809,679 + MMJJ_RS04335 0.24 +1.7
809,679 + MMJJ_RS04335 0.24 -0.5
809,688 + MMJJ_RS04335 0.25 -1.8
809,717 + MMJJ_RS04335 0.26 -1.6
809,774 - MMJJ_RS04335 0.29 +0.9
809,797 + MMJJ_RS04335 0.30 +0.9
809,873 + MMJJ_RS04335 0.34 +2.3
809,940 - MMJJ_RS04335 0.37 -1.4
810,004 - MMJJ_RS04335 0.40 -2.8
810,005 + MMJJ_RS04335 0.40 -1.2
810,005 + MMJJ_RS04335 0.40 +1.4
810,005 + MMJJ_RS04335 0.40 -1.4
810,033 - MMJJ_RS04335 0.42 -1.3
810,035 - MMJJ_RS04335 0.42 +1.2
810,035 - MMJJ_RS04335 0.42 +1.9
810,035 - MMJJ_RS04335 0.42 +1.2
810,036 + MMJJ_RS04335 0.42 +0.8
810,097 - MMJJ_RS04335 0.45 -2.3
810,098 + MMJJ_RS04335 0.45 +2.0
810,103 - MMJJ_RS04335 0.45 -1.0
810,104 + MMJJ_RS04335 0.45 +0.6
810,115 - MMJJ_RS04335 0.46 +2.3
810,115 - MMJJ_RS04335 0.46 +1.7
810,115 - MMJJ_RS04335 0.46 -2.2
810,116 + MMJJ_RS04335 0.46 +1.7
810,126 - MMJJ_RS04335 0.46 +1.4

Or see this region's nucleotide sequence