Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS04145 and MMJJ_RS04150 are separated by 9 nucleotides MMJJ_RS04150 and MMJJ_RS04155 are separated by 9 nucleotides MMJJ_RS04155 and MMJJ_RS04160 are separated by 78 nucleotides MMJJ_RS04160 and MMJJ_RS04165 are separated by 197 nucleotides
MMJJ_RS04145: MMJJ_RS04145 - glutamate-1-semialdehyde 2,1-aminomutase, at 770,396 to 771,679
_RS04145
MMJJ_RS04150: MMJJ_RS04150 - DUF5379 domain-containing protein, at 771,689 to 771,967
_RS04150
MMJJ_RS04155: MMJJ_RS04155 - hypothetical protein, at 771,977 to 772,216
_RS04155
MMJJ_RS04160: MMJJ_RS04160 - sodium/proline symporter PutP, at 772,295 to 773,773
_RS04160
MMJJ_RS04165: MMJJ_RS04165 - sodium:solute symporter, at 773,971 to 775,440
_RS04165
Position (kb) 772
773
774 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 771.303 kb on + strand, within MMJJ_RS04145 at 771.367 kb on - strand, within MMJJ_RS04145 at 771.367 kb on - strand, within MMJJ_RS04145 at 771.456 kb on + strand, within MMJJ_RS04145 at 771.458 kb on - strand, within MMJJ_RS04145 at 771.460 kb on - strand, within MMJJ_RS04145 at 771.500 kb on - strand, within MMJJ_RS04145 at 771.642 kb on + strand at 771.709 kb on + strand at 771.709 kb on + strand at 771.726 kb on + strand, within MMJJ_RS04150 at 771.752 kb on - strand, within MMJJ_RS04150 at 771.801 kb on + strand, within MMJJ_RS04150 at 771.827 kb on + strand, within MMJJ_RS04150 at 771.878 kb on - strand, within MMJJ_RS04150 at 771.897 kb on - strand at 771.973 kb on + strand at 771.973 kb on + strand at 771.979 kb on + strand at 772.043 kb on + strand, within MMJJ_RS04155 at 772.044 kb on - strand, within MMJJ_RS04155 at 772.044 kb on - strand, within MMJJ_RS04155 at 772.048 kb on - strand at 772.048 kb on - strand, within MMJJ_RS04155 at 772.048 kb on - strand, within MMJJ_RS04155 at 772.049 kb on + strand, within MMJJ_RS04155 at 772.049 kb on + strand, within MMJJ_RS04155 at 772.049 kb on + strand, within MMJJ_RS04155 at 772.049 kb on + strand, within MMJJ_RS04155 at 772.049 kb on + strand, within MMJJ_RS04155 at 772.049 kb on + strand, within MMJJ_RS04155 at 772.055 kb on - strand, within MMJJ_RS04155 at 772.061 kb on - strand at 772.067 kb on - strand, within MMJJ_RS04155 at 772.068 kb on + strand, within MMJJ_RS04155 at 772.068 kb on + strand at 772.068 kb on + strand, within MMJJ_RS04155 at 772.068 kb on + strand, within MMJJ_RS04155 at 772.079 kb on - strand, within MMJJ_RS04155 at 772.084 kb on + strand, within MMJJ_RS04155 at 772.099 kb on - strand, within MMJJ_RS04155 at 772.104 kb on + strand at 772.117 kb on - strand, within MMJJ_RS04155 at 772.117 kb on - strand, within MMJJ_RS04155 at 772.121 kb on - strand, within MMJJ_RS04155 at 772.173 kb on + strand, within MMJJ_RS04155 at 772.216 kb on - strand at 772.230 kb on + strand at 772.247 kb on - strand at 772.247 kb on - strand at 772.256 kb on - strand at 772.257 kb on + strand at 772.260 kb on - strand at 772.260 kb on - strand at 772.260 kb on - strand at 772.261 kb on + strand at 772.269 kb on - strand at 772.269 kb on - strand at 772.270 kb on + strand at 772.276 kb on - strand at 772.287 kb on - strand at 772.340 kb on - strand at 772.367 kb on - strand at 772.457 kb on - strand, within MMJJ_RS04160 at 772.617 kb on - strand, within MMJJ_RS04160 at 772.620 kb on - strand, within MMJJ_RS04160 at 772.621 kb on + strand, within MMJJ_RS04160 at 772.625 kb on - strand, within MMJJ_RS04160 at 772.736 kb on - strand, within MMJJ_RS04160 at 772.736 kb on - strand, within MMJJ_RS04160 at 772.838 kb on - strand, within MMJJ_RS04160 at 772.839 kb on + strand, within MMJJ_RS04160 at 772.839 kb on + strand, within MMJJ_RS04160 at 772.839 kb on + strand, within MMJJ_RS04160 at 772.906 kb on - strand, within MMJJ_RS04160 at 772.982 kb on + strand, within MMJJ_RS04160 at 772.988 kb on - strand, within MMJJ_RS04160 at 772.989 kb on + strand, within MMJJ_RS04160 at 773.014 kb on + strand, within MMJJ_RS04160 at 773.018 kb on - strand, within MMJJ_RS04160 at 773.051 kb on - strand, within MMJJ_RS04160 at 773.053 kb on - strand, within MMJJ_RS04160 at 773.054 kb on + strand, within MMJJ_RS04160 at 773.072 kb on - strand, within MMJJ_RS04160 at 773.105 kb on - strand, within MMJJ_RS04160 at 773.106 kb on + strand, within MMJJ_RS04160 at 773.106 kb on + strand, within MMJJ_RS04160 at 773.129 kb on + strand, within MMJJ_RS04160 at 773.164 kb on - strand, within MMJJ_RS04160 at 773.205 kb on + strand, within MMJJ_RS04160 at 773.248 kb on - strand, within MMJJ_RS04160 at 773.257 kb on - strand, within MMJJ_RS04160 at 773.262 kb on + strand, within MMJJ_RS04160 at 773.273 kb on - strand, within MMJJ_RS04160 at 773.274 kb on + strand, within MMJJ_RS04160 at 773.279 kb on + strand, within MMJJ_RS04160 at 773.291 kb on + strand, within MMJJ_RS04160 at 773.291 kb on + strand, within MMJJ_RS04160 at 773.293 kb on + strand, within MMJJ_RS04160 at 773.358 kb on + strand, within MMJJ_RS04160 at 773.447 kb on + strand, within MMJJ_RS04160 at 773.447 kb on + strand, within MMJJ_RS04160 at 773.449 kb on - strand, within MMJJ_RS04160 at 773.472 kb on - strand, within MMJJ_RS04160 at 773.479 kb on - strand, within MMJJ_RS04160 at 773.479 kb on - strand, within MMJJ_RS04160 at 773.479 kb on - strand, within MMJJ_RS04160 at 773.507 kb on - strand, within MMJJ_RS04160 at 773.512 kb on + strand, within MMJJ_RS04160 at 773.512 kb on + strand, within MMJJ_RS04160 at 773.512 kb on + strand, within MMJJ_RS04160 at 773.646 kb on + strand at 773.667 kb on - strand at 773.668 kb on + strand at 773.704 kb on - strand at 773.705 kb on + strand at 773.705 kb on + strand at 773.705 kb on + strand at 773.708 kb on - strand at 773.708 kb on - strand at 773.709 kb on + strand at 773.730 kb on - strand at 773.730 kb on - strand at 773.731 kb on + strand at 773.731 kb on + strand at 773.774 kb on + strand at 773.774 kb on + strand at 773.824 kb on - strand at 773.828 kb on + strand at 773.839 kb on - strand at 773.850 kb on - strand at 773.850 kb on - strand at 773.851 kb on + strand at 773.853 kb on - strand at 773.853 kb on - strand at 773.853 kb on - strand at 773.853 kb on - strand at 773.854 kb on + strand at 773.854 kb on + strand at 773.854 kb on + strand at 773.854 kb on + strand at 773.854 kb on + strand at 773.854 kb on + strand at 773.854 kb on + strand at 773.854 kb on + strand at 773.854 kb on + strand at 773.854 kb on + strand at 773.854 kb on + strand at 773.854 kb on + strand at 773.854 kb on + strand at 773.854 kb on + strand at 773.856 kb on + strand at 773.857 kb on - strand at 773.857 kb on - strand at 773.857 kb on - strand at 773.857 kb on - strand at 773.858 kb on + strand at 773.858 kb on + strand at 773.871 kb on + strand at 773.891 kb on + strand at 773.923 kb on - strand at 773.924 kb on + strand at 773.924 kb on + strand at 773.924 kb on + strand at 773.963 kb on + strand at 773.986 kb on - strand at 773.987 kb on + strand at 773.987 kb on + strand at 773.987 kb on + strand at 773.991 kb on + strand at 774.040 kb on - strand at 774.113 kb on - strand at 774.191 kb on + strand, within MMJJ_RS04165 at 774.211 kb on - strand, within MMJJ_RS04165 at 774.257 kb on + strand, within MMJJ_RS04165 at 774.257 kb on + strand, within MMJJ_RS04165 at 774.263 kb on - strand, within MMJJ_RS04165 at 774.264 kb on + strand, within MMJJ_RS04165 at 774.278 kb on + strand, within MMJJ_RS04165 at 774.298 kb on - strand, within MMJJ_RS04165 at 774.298 kb on - strand, within MMJJ_RS04165 at 774.299 kb on + strand, within MMJJ_RS04165 at 774.299 kb on + strand, within MMJJ_RS04165 at 774.299 kb on + strand, within MMJJ_RS04165 at 774.299 kb on + strand, within MMJJ_RS04165 at 774.345 kb on - strand, within MMJJ_RS04165 at 774.345 kb on - strand, within MMJJ_RS04165 at 774.385 kb on - strand, within MMJJ_RS04165 at 774.424 kb on - strand, within MMJJ_RS04165 at 774.430 kb on + strand, within MMJJ_RS04165 at 774.449 kb on + strand, within MMJJ_RS04165 at 774.463 kb on - strand, within MMJJ_RS04165 at 774.464 kb on + strand, within MMJJ_RS04165 at 774.499 kb on - strand, within MMJJ_RS04165 at 774.511 kb on - strand, within MMJJ_RS04165 at 774.511 kb on - strand, within MMJJ_RS04165 at 774.511 kb on - strand, within MMJJ_RS04165 at 774.511 kb on - strand, within MMJJ_RS04165 at 774.512 kb on + strand, within MMJJ_RS04165 at 774.512 kb on + strand, within MMJJ_RS04165 at 774.577 kb on - strand, within MMJJ_RS04165 at 774.578 kb on + strand, within MMJJ_RS04165 at 774.589 kb on + strand, within MMJJ_RS04165 at 774.589 kb on + strand, within MMJJ_RS04165 at 774.590 kb on - strand, within MMJJ_RS04165 at 774.590 kb on - strand, within MMJJ_RS04165 at 774.591 kb on + strand, within MMJJ_RS04165 at 774.607 kb on + strand, within MMJJ_RS04165 at 774.613 kb on + strand, within MMJJ_RS04165 at 774.667 kb on - strand, within MMJJ_RS04165 at 774.681 kb on - strand, within MMJJ_RS04165 at 774.682 kb on + strand, within MMJJ_RS04165 at 774.685 kb on - strand, within MMJJ_RS04165 at 774.764 kb on + strand, within MMJJ_RS04165
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE remove 771,303 + MMJJ_RS04145 0.71 -3.2 771,367 - MMJJ_RS04145 0.76 -2.5 771,367 - MMJJ_RS04145 0.76 -2.7 771,456 + MMJJ_RS04145 0.83 -0.9 771,458 - MMJJ_RS04145 0.83 -1.7 771,460 - MMJJ_RS04145 0.83 -3.3 771,500 - MMJJ_RS04145 0.86 -3.0 771,642 + -1.9 771,709 + -1.5 771,709 + -1.9 771,726 + MMJJ_RS04150 0.13 -0.9 771,752 - MMJJ_RS04150 0.23 -2.5 771,801 + MMJJ_RS04150 0.40 -1.5 771,827 + MMJJ_RS04150 0.49 -3.2 771,878 - MMJJ_RS04150 0.68 -2.0 771,897 - +0.1 771,973 + -0.9 771,973 + -3.6 771,979 + -3.1 772,043 + MMJJ_RS04155 0.28 -3.5 772,044 - MMJJ_RS04155 0.28 -3.0 772,044 - MMJJ_RS04155 0.28 -0.9 772,048 - -0.9 772,048 - MMJJ_RS04155 0.30 -1.5 772,048 - MMJJ_RS04155 0.30 -1.2 772,049 + MMJJ_RS04155 0.30 -1.2 772,049 + MMJJ_RS04155 0.30 -2.2 772,049 + MMJJ_RS04155 0.30 -0.6 772,049 + MMJJ_RS04155 0.30 -0.9 772,049 + MMJJ_RS04155 0.30 -2.7 772,049 + MMJJ_RS04155 0.30 -2.2 772,055 - MMJJ_RS04155 0.33 -2.5 772,061 - -0.9 772,067 - MMJJ_RS04155 0.38 -3.3 772,068 + MMJJ_RS04155 0.38 +0.1 772,068 + -0.9 772,068 + MMJJ_RS04155 0.38 -2.9 772,068 + MMJJ_RS04155 0.38 -2.2 772,079 - MMJJ_RS04155 0.42 -2.7 772,084 + MMJJ_RS04155 0.45 -0.9 772,099 - MMJJ_RS04155 0.51 -3.8 772,104 + -1.5 772,117 - MMJJ_RS04155 0.58 -1.9 772,117 - MMJJ_RS04155 0.58 -1.6 772,121 - MMJJ_RS04155 0.60 -0.3 772,173 + MMJJ_RS04155 0.82 -2.9 772,216 - -1.9 772,230 + -2.2 772,247 - +1.3 772,247 - +0.6 772,256 - -0.5 772,257 + -2.9 772,260 - +0.3 772,260 - -0.9 772,260 - +0.7 772,261 + -1.5 772,269 - +0.5 772,269 - -2.1 772,270 + +1.3 772,276 - +2.1 772,287 - +1.1 772,340 - +0.4 772,367 - +1.3 772,457 - MMJJ_RS04160 0.11 -0.6 772,617 - MMJJ_RS04160 0.22 +1.3 772,620 - MMJJ_RS04160 0.22 +1.1 772,621 + MMJJ_RS04160 0.22 -1.5 772,625 - MMJJ_RS04160 0.22 -2.3 772,736 - MMJJ_RS04160 0.30 -3.0 772,736 - MMJJ_RS04160 0.30 +1.0 772,838 - MMJJ_RS04160 0.37 -3.0 772,839 + MMJJ_RS04160 0.37 +1.6 772,839 + MMJJ_RS04160 0.37 +0.8 772,839 + MMJJ_RS04160 0.37 -1.5 772,906 - MMJJ_RS04160 0.41 -0.2 772,982 + MMJJ_RS04160 0.46 +0.6 772,988 - MMJJ_RS04160 0.47 +1.4 772,989 + MMJJ_RS04160 0.47 +1.8 773,014 + MMJJ_RS04160 0.49 +0.9 773,018 - MMJJ_RS04160 0.49 +1.0 773,051 - MMJJ_RS04160 0.51 +0.7 773,053 - MMJJ_RS04160 0.51 +1.3 773,054 + MMJJ_RS04160 0.51 -1.2 773,072 - MMJJ_RS04160 0.53 -1.3 773,105 - MMJJ_RS04160 0.55 -0.6 773,106 + MMJJ_RS04160 0.55 -2.8 773,106 + MMJJ_RS04160 0.55 -2.8 773,129 + MMJJ_RS04160 0.56 +0.7 773,164 - MMJJ_RS04160 0.59 -2.1 773,205 + MMJJ_RS04160 0.62 +0.8 773,248 - MMJJ_RS04160 0.64 -2.2 773,257 - MMJJ_RS04160 0.65 -2.4 773,262 + MMJJ_RS04160 0.65 +1.4 773,273 - MMJJ_RS04160 0.66 +0.3 773,274 + MMJJ_RS04160 0.66 -3.2 773,279 + MMJJ_RS04160 0.67 -2.2 773,291 + MMJJ_RS04160 0.67 +0.2 773,291 + MMJJ_RS04160 0.67 -1.2 773,293 + MMJJ_RS04160 0.67 -2.7 773,358 + MMJJ_RS04160 0.72 +0.5 773,447 + MMJJ_RS04160 0.78 -2.0 773,447 + MMJJ_RS04160 0.78 +0.5 773,449 - MMJJ_RS04160 0.78 -1.9 773,472 - MMJJ_RS04160 0.80 +1.2 773,479 - MMJJ_RS04160 0.80 -3.0 773,479 - MMJJ_RS04160 0.80 +1.3 773,479 - MMJJ_RS04160 0.80 -2.2 773,507 - MMJJ_RS04160 0.82 -1.5 773,512 + MMJJ_RS04160 0.82 -2.8 773,512 + MMJJ_RS04160 0.82 -3.5 773,512 + MMJJ_RS04160 0.82 +0.6 773,646 + -2.1 773,667 - -1.4 773,668 + -0.5 773,704 - +0.6 773,705 + -3.0 773,705 + -2.1 773,705 + -2.3 773,708 - +1.3 773,708 - -0.9 773,709 + -1.7 773,730 - +0.5 773,730 - +1.2 773,731 + -2.5 773,731 + +0.1 773,774 + -1.5 773,774 + -2.9 773,824 - -1.7 773,828 + +0.7 773,839 - +0.8 773,850 - +0.0 773,850 - -1.9 773,851 + -1.2 773,853 - +0.9 773,853 - +1.0 773,853 - +1.2 773,853 - -2.6 773,854 + -1.2 773,854 + +1.3 773,854 + -2.4 773,854 + -0.6 773,854 + +0.6 773,854 + -0.6 773,854 + +0.7 773,854 + +0.1 773,854 + +1.1 773,854 + -1.9 773,854 + +1.8 773,854 + +0.9 773,854 + -0.5 773,854 + +1.4 773,856 + -1.7 773,857 - +2.5 773,857 - -0.0 773,857 - -1.7 773,857 - -3.0 773,858 + +0.8 773,858 + +0.5 773,871 + -1.5 773,891 + -1.5 773,923 - -4.2 773,924 + -1.5 773,924 + -2.2 773,924 + -1.1 773,963 + +1.4 773,986 - -0.1 773,987 + +1.1 773,987 + +2.2 773,987 + -1.3 773,991 + -2.1 774,040 - +1.4 774,113 - -0.2 774,191 + MMJJ_RS04165 0.15 +0.9 774,211 - MMJJ_RS04165 0.16 -0.2 774,257 + MMJJ_RS04165 0.19 +1.8 774,257 + MMJJ_RS04165 0.19 -1.3 774,263 - MMJJ_RS04165 0.20 +1.6 774,264 + MMJJ_RS04165 0.20 -1.5 774,278 + MMJJ_RS04165 0.21 +0.4 774,298 - MMJJ_RS04165 0.22 +0.3 774,298 - MMJJ_RS04165 0.22 -1.8 774,299 + MMJJ_RS04165 0.22 +0.9 774,299 + MMJJ_RS04165 0.22 +1.4 774,299 + MMJJ_RS04165 0.22 +0.9 774,299 + MMJJ_RS04165 0.22 +1.6 774,345 - MMJJ_RS04165 0.25 +0.8 774,345 - MMJJ_RS04165 0.25 +0.9 774,385 - MMJJ_RS04165 0.28 +1.1 774,424 - MMJJ_RS04165 0.31 -2.0 774,430 + MMJJ_RS04165 0.31 -3.7 774,449 + MMJJ_RS04165 0.33 -1.8 774,463 - MMJJ_RS04165 0.33 +0.8 774,464 + MMJJ_RS04165 0.34 +1.2 774,499 - MMJJ_RS04165 0.36 -1.5 774,511 - MMJJ_RS04165 0.37 -1.4 774,511 - MMJJ_RS04165 0.37 +1.9 774,511 - MMJJ_RS04165 0.37 +1.1 774,511 - MMJJ_RS04165 0.37 +0.1 774,512 + MMJJ_RS04165 0.37 -1.0 774,512 + MMJJ_RS04165 0.37 +1.8 774,577 - MMJJ_RS04165 0.41 +1.4 774,578 + MMJJ_RS04165 0.41 -2.5 774,589 + MMJJ_RS04165 0.42 +1.1 774,589 + MMJJ_RS04165 0.42 -0.6 774,590 - MMJJ_RS04165 0.42 +1.7 774,590 - MMJJ_RS04165 0.42 -2.5 774,591 + MMJJ_RS04165 0.42 +1.2 774,607 + MMJJ_RS04165 0.43 +1.3 774,613 + MMJJ_RS04165 0.44 +1.1 774,667 - MMJJ_RS04165 0.47 -0.5 774,681 - MMJJ_RS04165 0.48 -2.3 774,682 + MMJJ_RS04165 0.48 -1.7 774,685 - MMJJ_RS04165 0.49 +1.6 774,764 + MMJJ_RS04165 0.54 +0.1
Or see this region's nucleotide sequence