Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS03635 and MMJJ_RS03640 are separated by 218 nucleotides MMJJ_RS03640 and MMJJ_RS03645 are separated by 66 nucleotides MMJJ_RS03645 and MMJJ_RS03650 are separated by 68 nucleotides
MMJJ_RS03635: MMJJ_RS03635 - methionine--tRNA ligase, at 677,237 to 679,228
_RS03635
MMJJ_RS03640: MMJJ_RS03640 - type II glyceraldehyde-3-phosphate dehydrogenase, at 679,447 to 680,469
_RS03640
MMJJ_RS03645: MMJJ_RS03645 - hypothetical protein, at 680,536 to 680,916
_RS03645
MMJJ_RS03650: MMJJ_RS03650 - class I SAM-dependent methyltransferase family protein, at 680,985 to 682,013
_RS03650
Position (kb) 679
680
681 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 678.492 kb on - strand, within MMJJ_RS03635 at 678.536 kb on - strand, within MMJJ_RS03635 at 678.819 kb on - strand at 679.137 kb on - strand at 679.155 kb on - strand at 679.268 kb on - strand at 679.297 kb on - strand at 679.297 kb on - strand at 679.298 kb on + strand at 679.298 kb on + strand at 679.302 kb on - strand at 679.302 kb on - strand at 679.302 kb on - strand at 679.302 kb on - strand at 679.305 kb on - strand at 679.306 kb on + strand at 679.306 kb on + strand at 679.308 kb on - strand at 679.308 kb on - strand at 679.308 kb on - strand at 679.308 kb on - strand at 679.309 kb on + strand at 679.312 kb on - strand at 679.350 kb on + strand at 679.351 kb on - strand at 679.351 kb on - strand at 679.351 kb on - strand at 679.352 kb on + strand at 679.356 kb on - strand at 679.356 kb on - strand at 679.356 kb on - strand at 679.357 kb on + strand at 679.360 kb on + strand at 679.361 kb on - strand at 679.361 kb on - strand at 679.361 kb on - strand at 679.361 kb on - strand at 679.361 kb on - strand at 679.361 kb on - strand at 679.361 kb on - strand at 679.362 kb on + strand at 679.362 kb on + strand at 679.362 kb on + strand at 679.362 kb on + strand at 679.366 kb on + strand at 679.366 kb on + strand at 679.372 kb on - strand at 679.379 kb on + strand at 679.379 kb on + strand at 679.400 kb on + strand at 679.411 kb on + strand at 679.850 kb on + strand, within MMJJ_RS03640 at 679.862 kb on - strand, within MMJJ_RS03640 at 679.887 kb on - strand, within MMJJ_RS03640 at 679.897 kb on - strand, within MMJJ_RS03640 at 680.391 kb on - strand at 680.459 kb on - strand at 680.491 kb on + strand at 680.494 kb on - strand at 680.508 kb on - strand at 680.524 kb on + strand at 680.542 kb on - strand at 680.547 kb on + strand at 680.612 kb on - strand at 680.617 kb on + strand, within MMJJ_RS03645 at 680.619 kb on + strand at 680.645 kb on - strand, within MMJJ_RS03645 at 680.937 kb on - strand at 680.937 kb on - strand at 680.937 kb on - strand at 680.937 kb on - strand at 680.937 kb on - strand at 680.938 kb on + strand at 680.938 kb on + strand at 680.938 kb on + strand at 680.938 kb on + strand at 680.939 kb on - strand at 680.939 kb on - strand at 680.940 kb on + strand at 680.948 kb on - strand at 680.948 kb on - strand at 680.954 kb on - strand at 680.954 kb on - strand at 680.955 kb on + strand at 680.955 kb on + strand at 680.955 kb on + strand at 680.958 kb on - strand at 680.983 kb on - strand at 681.055 kb on - strand at 681.056 kb on + strand at 681.059 kb on - strand at 681.062 kb on - strand at 681.099 kb on - strand, within MMJJ_RS03650 at 681.286 kb on + strand, within MMJJ_RS03650
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE remove 678,492 - MMJJ_RS03635 0.63 +0.2 678,536 - MMJJ_RS03635 0.65 -2.6 678,819 - -1.8 679,137 - -1.4 679,155 - -0.8 679,268 - -0.8 679,297 - -1.6 679,297 - -2.1 679,298 + -1.1 679,298 + -2.5 679,302 - -1.0 679,302 - -3.1 679,302 - -2.1 679,302 - +1.2 679,305 - -2.3 679,306 + -3.4 679,306 + +1.4 679,308 - -4.1 679,308 - -1.2 679,308 - -3.7 679,308 - +1.8 679,309 + +0.6 679,312 - -0.8 679,350 + +1.0 679,351 - +1.4 679,351 - -0.6 679,351 - +1.1 679,352 + +0.7 679,356 - -0.6 679,356 - -1.8 679,356 - +1.4 679,357 + +0.2 679,360 + -2.1 679,361 - -1.7 679,361 - +0.3 679,361 - +1.3 679,361 - +1.1 679,361 - -0.4 679,361 - +0.6 679,361 - -1.4 679,362 + +1.7 679,362 + -1.0 679,362 + -0.9 679,362 + +0.3 679,366 + -4.0 679,366 + -0.7 679,372 - -1.1 679,379 + +0.2 679,379 + -2.4 679,400 + -0.8 679,411 + +0.2 679,850 + MMJJ_RS03640 0.39 -2.1 679,862 - MMJJ_RS03640 0.41 -1.4 679,887 - MMJJ_RS03640 0.43 +0.2 679,897 - MMJJ_RS03640 0.44 -0.8 680,391 - -2.1 680,459 - +1.3 680,491 + -1.5 680,494 - -1.5 680,508 - -2.4 680,524 + -0.1 680,542 - -1.8 680,547 + +0.2 680,612 - +0.2 680,617 + MMJJ_RS03645 0.21 -1.8 680,619 + +0.2 680,645 - MMJJ_RS03645 0.29 -0.8 680,937 - -1.4 680,937 - +0.7 680,937 - -2.1 680,937 - +1.2 680,937 - -0.5 680,938 + -1.7 680,938 + -2.1 680,938 + -3.7 680,938 + -0.7 680,939 - -0.6 680,939 - -2.4 680,940 + +0.7 680,948 - +0.5 680,948 - +0.3 680,954 - +1.5 680,954 - +0.5 680,955 + +0.5 680,955 + +1.8 680,955 + -1.0 680,958 - -2.1 680,983 - -3.0 681,055 - -2.1 681,056 + -2.1 681,059 - -1.4 681,062 - -3.1 681,099 - MMJJ_RS03650 0.11 +0.2 681,286 + MMJJ_RS03650 0.29 +0.9
Or see this region's nucleotide sequence