Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS08625 and MMJJ_RS08630 are separated by 13 nucleotides MMJJ_RS08630 and MMJJ_RS08635 are separated by 0 nucleotides MMJJ_RS08635 and MMJJ_RS08640 are separated by 12 nucleotides MMJJ_RS08640 and MMJJ_RS08645 are separated by 57 nucleotides MMJJ_RS08645 and MMJJ_RS08650 are separated by 50 nucleotides
MMJJ_RS08625: MMJJ_RS08625 - magnesium/cobalt transporter CorA, at 1,588,780 to 1,589,730
_RS08625
MMJJ_RS08630: MMJJ_RS08630 - signal recognition particle subunit SRP19/SEC65 family protein, at 1,589,744 to 1,590,013
_RS08630
MMJJ_RS08635: MMJJ_RS08635 - hypothetical protein, at 1,590,014 to 1,590,325
_RS08635
MMJJ_RS08640: MMJJ_RS08640 - ADP-forming succinate--CoA ligase subunit beta, at 1,590,338 to 1,591,426
_RS08640
MMJJ_RS08645: MMJJ_RS08645 - aspartate carbamoyltransferase regulatory subunit, at 1,591,484 to 1,591,930
_RS08645
MMJJ_RS08650: MMJJ_RS08650 - TfuA-like protein, at 1,591,981 to 1,592,625
_RS08650
Position (kb)
1590
1591
1592 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1589.346 kb on - strand, within MMJJ_RS08625 at 1589.346 kb on - strand, within MMJJ_RS08625 at 1589.346 kb on - strand, within MMJJ_RS08625 at 1589.346 kb on - strand, within MMJJ_RS08625 at 1589.348 kb on - strand, within MMJJ_RS08625 at 1589.364 kb on - strand, within MMJJ_RS08625 at 1589.365 kb on + strand, within MMJJ_RS08625 at 1589.412 kb on - strand, within MMJJ_RS08625 at 1589.412 kb on - strand, within MMJJ_RS08625 at 1589.413 kb on + strand, within MMJJ_RS08625 at 1589.413 kb on + strand at 1589.432 kb on - strand, within MMJJ_RS08625 at 1589.437 kb on - strand, within MMJJ_RS08625 at 1589.474 kb on + strand, within MMJJ_RS08625 at 1589.673 kb on - strand at 1589.774 kb on + strand, within MMJJ_RS08630 at 1589.887 kb on - strand, within MMJJ_RS08630 at 1589.888 kb on + strand, within MMJJ_RS08630 at 1589.888 kb on + strand, within MMJJ_RS08630 at 1589.888 kb on + strand, within MMJJ_RS08630 at 1589.888 kb on + strand, within MMJJ_RS08630 at 1589.888 kb on + strand, within MMJJ_RS08630 at 1589.909 kb on - strand, within MMJJ_RS08630 at 1589.943 kb on - strand, within MMJJ_RS08630 at 1589.943 kb on - strand, within MMJJ_RS08630 at 1589.943 kb on - strand, within MMJJ_RS08630 at 1589.943 kb on - strand, within MMJJ_RS08630 at 1589.944 kb on + strand, within MMJJ_RS08630 at 1589.944 kb on + strand, within MMJJ_RS08630 at 1589.944 kb on + strand, within MMJJ_RS08630 at 1589.965 kb on - strand, within MMJJ_RS08630 at 1589.965 kb on - strand, within MMJJ_RS08630 at 1589.966 kb on + strand, within MMJJ_RS08630 at 1589.994 kb on - strand at 1589.994 kb on - strand at 1589.994 kb on - strand at 1589.994 kb on - strand at 1589.995 kb on + strand at 1589.998 kb on - strand at 1589.998 kb on - strand at 1589.998 kb on - strand at 1589.998 kb on - strand at 1589.999 kb on + strand at 1590.001 kb on - strand at 1590.011 kb on - strand at 1590.012 kb on + strand at 1590.012 kb on + strand at 1590.012 kb on + strand at 1590.140 kb on - strand, within MMJJ_RS08635 at 1590.140 kb on - strand, within MMJJ_RS08635 at 1590.140 kb on - strand, within MMJJ_RS08635 at 1590.141 kb on + strand, within MMJJ_RS08635 at 1590.141 kb on + strand, within MMJJ_RS08635 at 1590.141 kb on + strand, within MMJJ_RS08635 at 1590.141 kb on + strand, within MMJJ_RS08635 at 1590.141 kb on + strand, within MMJJ_RS08635 at 1590.145 kb on + strand, within MMJJ_RS08635 at 1590.170 kb on - strand, within MMJJ_RS08635 at 1590.170 kb on - strand, within MMJJ_RS08635 at 1590.171 kb on + strand, within MMJJ_RS08635 at 1590.171 kb on + strand, within MMJJ_RS08635 at 1590.171 kb on + strand, within MMJJ_RS08635 at 1590.232 kb on - strand, within MMJJ_RS08635 at 1590.233 kb on + strand, within MMJJ_RS08635 at 1590.233 kb on + strand, within MMJJ_RS08635 at 1590.234 kb on - strand, within MMJJ_RS08635 at 1590.235 kb on + strand, within MMJJ_RS08635 at 1590.235 kb on + strand, within MMJJ_RS08635 at 1590.244 kb on - strand, within MMJJ_RS08635 at 1590.253 kb on - strand, within MMJJ_RS08635 at 1590.253 kb on - strand, within MMJJ_RS08635 at 1590.253 kb on - strand, within MMJJ_RS08635 at 1590.258 kb on + strand, within MMJJ_RS08635 at 1590.277 kb on + strand, within MMJJ_RS08635 at 1590.277 kb on + strand, within MMJJ_RS08635 at 1590.281 kb on + strand, within MMJJ_RS08635 at 1590.281 kb on + strand, within MMJJ_RS08635 at 1591.496 kb on - strand at 1591.496 kb on - strand at 1591.496 kb on - strand at 1591.498 kb on - strand at 1591.683 kb on + strand, within MMJJ_RS08645 at 1591.953 kb on - strand at 1591.997 kb on - strand at 1591.997 kb on - strand at 1591.997 kb on - strand at 1591.997 kb on - strand at 1591.998 kb on + strand at 1591.998 kb on + strand at 1591.998 kb on + strand at 1591.998 kb on + strand at 1592.003 kb on + strand at 1592.041 kb on - strand at 1592.041 kb on - strand at 1592.041 kb on - strand at 1592.043 kb on - strand at 1592.043 kb on - strand at 1592.044 kb on + strand at 1592.130 kb on - strand, within MMJJ_RS08650 at 1592.131 kb on + strand, within MMJJ_RS08650 at 1592.131 kb on + strand, within MMJJ_RS08650 at 1592.131 kb on + strand, within MMJJ_RS08650 at 1592.141 kb on - strand, within MMJJ_RS08650 at 1592.141 kb on - strand, within MMJJ_RS08650 at 1592.142 kb on + strand, within MMJJ_RS08650 at 1592.142 kb on + strand, within MMJJ_RS08650 at 1592.142 kb on + strand, within MMJJ_RS08650 at 1592.143 kb on - strand, within MMJJ_RS08650 at 1592.143 kb on - strand, within MMJJ_RS08650 at 1592.143 kb on - strand, within MMJJ_RS08650 at 1592.144 kb on + strand, within MMJJ_RS08650 at 1592.161 kb on + strand, within MMJJ_RS08650 at 1592.211 kb on - strand, within MMJJ_RS08650 at 1592.257 kb on - strand, within MMJJ_RS08650 at 1592.263 kb on + strand, within MMJJ_RS08650 at 1592.263 kb on + strand, within MMJJ_RS08650 at 1592.274 kb on - strand, within MMJJ_RS08650 at 1592.275 kb on + strand, within MMJJ_RS08650 at 1592.275 kb on + strand, within MMJJ_RS08650 at 1592.302 kb on - strand, within MMJJ_RS08650 at 1592.303 kb on + strand, within MMJJ_RS08650 at 1592.317 kb on - strand, within MMJJ_RS08650 at 1592.322 kb on - strand, within MMJJ_RS08650 at 1592.356 kb on + strand, within MMJJ_RS08650 at 1592.375 kb on + strand, within MMJJ_RS08650 at 1592.401 kb on - strand, within MMJJ_RS08650 at 1592.401 kb on - strand, within MMJJ_RS08650 at 1592.406 kb on - strand, within MMJJ_RS08650 at 1592.407 kb on + strand, within MMJJ_RS08650 at 1592.407 kb on + strand, within MMJJ_RS08650 at 1592.407 kb on + strand, within MMJJ_RS08650 at 1592.409 kb on - strand, within MMJJ_RS08650 at 1592.410 kb on + strand, within MMJJ_RS08650 at 1592.412 kb on - strand, within MMJJ_RS08650
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE remove 1,589,346 - MMJJ_RS08625 0.60 -2.5 1,589,346 - MMJJ_RS08625 0.60 -2.5 1,589,346 - MMJJ_RS08625 0.60 -1.5 1,589,346 - MMJJ_RS08625 0.60 -2.0 1,589,348 - MMJJ_RS08625 0.60 -1.4 1,589,364 - MMJJ_RS08625 0.61 -3.5 1,589,365 + MMJJ_RS08625 0.62 -1.5 1,589,412 - MMJJ_RS08625 0.66 -2.0 1,589,412 - MMJJ_RS08625 0.66 -2.3 1,589,413 + MMJJ_RS08625 0.67 -1.0 1,589,413 + -2.0 1,589,432 - MMJJ_RS08625 0.69 -3.1 1,589,437 - MMJJ_RS08625 0.69 -2.3 1,589,474 + MMJJ_RS08625 0.73 -1.0 1,589,673 - -2.8 1,589,774 + MMJJ_RS08630 0.11 -3.0 1,589,887 - MMJJ_RS08630 0.53 -1.5 1,589,888 + MMJJ_RS08630 0.53 +0.2 1,589,888 + MMJJ_RS08630 0.53 +2.0 1,589,888 + MMJJ_RS08630 0.53 +0.7 1,589,888 + MMJJ_RS08630 0.53 +1.4 1,589,888 + MMJJ_RS08630 0.53 -1.0 1,589,909 - MMJJ_RS08630 0.61 -1.2 1,589,943 - MMJJ_RS08630 0.74 +1.0 1,589,943 - MMJJ_RS08630 0.74 +1.9 1,589,943 - MMJJ_RS08630 0.74 +1.0 1,589,943 - MMJJ_RS08630 0.74 -0.5 1,589,944 + MMJJ_RS08630 0.74 +1.6 1,589,944 + MMJJ_RS08630 0.74 -1.7 1,589,944 + MMJJ_RS08630 0.74 -2.0 1,589,965 - MMJJ_RS08630 0.82 +0.5 1,589,965 - MMJJ_RS08630 0.82 +0.4 1,589,966 + MMJJ_RS08630 0.82 +1.0 1,589,994 - +1.2 1,589,994 - +0.0 1,589,994 - -0.8 1,589,994 - +0.5 1,589,995 + +0.9 1,589,998 - +2.0 1,589,998 - +1.5 1,589,998 - -3.3 1,589,998 - +0.8 1,589,999 + +2.3 1,590,001 - +1.6 1,590,011 - -2.4 1,590,012 + -2.3 1,590,012 + +1.3 1,590,012 + -1.6 1,590,140 - MMJJ_RS08635 0.40 +1.1 1,590,140 - MMJJ_RS08635 0.40 +1.8 1,590,140 - MMJJ_RS08635 0.40 +1.4 1,590,141 + MMJJ_RS08635 0.41 -1.2 1,590,141 + MMJJ_RS08635 0.41 -2.1 1,590,141 + MMJJ_RS08635 0.41 +0.5 1,590,141 + MMJJ_RS08635 0.41 -0.1 1,590,141 + MMJJ_RS08635 0.41 -3.6 1,590,145 + MMJJ_RS08635 0.42 -3.3 1,590,170 - MMJJ_RS08635 0.50 -0.7 1,590,170 - MMJJ_RS08635 0.50 +1.2 1,590,171 + MMJJ_RS08635 0.50 -1.0 1,590,171 + MMJJ_RS08635 0.50 +2.0 1,590,171 + MMJJ_RS08635 0.50 -1.8 1,590,232 - MMJJ_RS08635 0.70 +1.2 1,590,233 + MMJJ_RS08635 0.70 +1.8 1,590,233 + MMJJ_RS08635 0.70 -0.9 1,590,234 - MMJJ_RS08635 0.71 -0.7 1,590,235 + MMJJ_RS08635 0.71 +1.1 1,590,235 + MMJJ_RS08635 0.71 -1.4 1,590,244 - MMJJ_RS08635 0.74 -2.5 1,590,253 - MMJJ_RS08635 0.77 +1.0 1,590,253 - MMJJ_RS08635 0.77 -1.5 1,590,253 - MMJJ_RS08635 0.77 +1.0 1,590,258 + MMJJ_RS08635 0.78 -4.2 1,590,277 + MMJJ_RS08635 0.84 +0.5 1,590,277 + MMJJ_RS08635 0.84 -2.8 1,590,281 + MMJJ_RS08635 0.86 +1.5 1,590,281 + MMJJ_RS08635 0.86 +2.0 1,591,496 - -2.3 1,591,496 - -2.5 1,591,496 - -0.8 1,591,498 - -1.2 1,591,683 + MMJJ_RS08645 0.45 -2.0 1,591,953 - -2.0 1,591,997 - -1.3 1,591,997 - +2.9 1,591,997 - -2.2 1,591,997 - +0.4 1,591,998 + +0.0 1,591,998 + +0.5 1,591,998 + +1.3 1,591,998 + +1.4 1,592,003 + +1.1 1,592,041 - +1.6 1,592,041 - -1.0 1,592,041 - +0.6 1,592,043 - -0.5 1,592,043 - +0.3 1,592,044 + -0.7 1,592,130 - MMJJ_RS08650 0.23 -0.7 1,592,131 + MMJJ_RS08650 0.23 +1.9 1,592,131 + MMJJ_RS08650 0.23 -1.8 1,592,131 + MMJJ_RS08650 0.23 -0.7 1,592,141 - MMJJ_RS08650 0.25 -1.5 1,592,141 - MMJJ_RS08650 0.25 +0.5 1,592,142 + MMJJ_RS08650 0.25 -1.1 1,592,142 + MMJJ_RS08650 0.25 -0.1 1,592,142 + MMJJ_RS08650 0.25 +0.6 1,592,143 - MMJJ_RS08650 0.25 -2.4 1,592,143 - MMJJ_RS08650 0.25 +0.4 1,592,143 - MMJJ_RS08650 0.25 +0.9 1,592,144 + MMJJ_RS08650 0.25 -1.4 1,592,161 + MMJJ_RS08650 0.28 +0.7 1,592,211 - MMJJ_RS08650 0.36 +0.7 1,592,257 - MMJJ_RS08650 0.43 -2.8 1,592,263 + MMJJ_RS08650 0.44 -2.5 1,592,263 + MMJJ_RS08650 0.44 -0.6 1,592,274 - MMJJ_RS08650 0.45 +0.7 1,592,275 + MMJJ_RS08650 0.46 +0.8 1,592,275 + MMJJ_RS08650 0.46 +0.6 1,592,302 - MMJJ_RS08650 0.50 -1.9 1,592,303 + MMJJ_RS08650 0.50 -0.2 1,592,317 - MMJJ_RS08650 0.52 -1.1 1,592,322 - MMJJ_RS08650 0.53 +0.0 1,592,356 + MMJJ_RS08650 0.58 -2.2 1,592,375 + MMJJ_RS08650 0.61 -1.2 1,592,401 - MMJJ_RS08650 0.65 -1.7 1,592,401 - MMJJ_RS08650 0.65 +1.0 1,592,406 - MMJJ_RS08650 0.66 -1.0 1,592,407 + MMJJ_RS08650 0.66 +0.6 1,592,407 + MMJJ_RS08650 0.66 +0.1 1,592,407 + MMJJ_RS08650 0.66 -1.9 1,592,409 - MMJJ_RS08650 0.66 -0.3 1,592,410 + MMJJ_RS08650 0.67 +1.2 1,592,412 - MMJJ_RS08650 0.67 -1.8
Or see this region's nucleotide sequence