Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS08010 and MMJJ_RS08015 are separated by 20 nucleotides MMJJ_RS08015 and MMJJ_RS08020 are separated by 28 nucleotides MMJJ_RS08020 and MMJJ_RS08025 are separated by 72 nucleotides MMJJ_RS08025 and MMJJ_RS08030 are separated by 94 nucleotides
MMJJ_RS08010: MMJJ_RS08010 - phosphoadenosine phosphosulfate reductase family protein, at 1,467,206 to 1,468,096
_RS08010
MMJJ_RS08015: MMJJ_RS08015 - HAD family hydrolase, at 1,468,117 to 1,468,566
_RS08015
MMJJ_RS08020: MMJJ_RS08020 - nucleotidyltransferase domain-containing protein, at 1,468,595 to 1,469,653
_RS08020
MMJJ_RS08025: MMJJ_RS08025 - hypothetical protein, at 1,469,726 to 1,470,028
_RS08025
MMJJ_RS08030: MMJJ_RS08030 - hypothetical protein, at 1,470,123 to 1,470,770
_RS08030
Position (kb)
1468
1469
1470 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 1467.621 kb on - strand, within MMJJ_RS08010 at 1467.675 kb on + strand, within MMJJ_RS08010 at 1467.675 kb on + strand, within MMJJ_RS08010 at 1467.810 kb on - strand, within MMJJ_RS08010 at 1467.837 kb on - strand, within MMJJ_RS08010 at 1467.838 kb on + strand, within MMJJ_RS08010 at 1467.838 kb on + strand, within MMJJ_RS08010 at 1467.841 kb on - strand, within MMJJ_RS08010 at 1467.843 kb on - strand, within MMJJ_RS08010 at 1467.843 kb on - strand, within MMJJ_RS08010 at 1467.844 kb on + strand, within MMJJ_RS08010 at 1467.951 kb on - strand, within MMJJ_RS08010 at 1468.039 kb on + strand at 1468.138 kb on + strand at 1468.205 kb on + strand, within MMJJ_RS08015 at 1468.243 kb on - strand, within MMJJ_RS08015 at 1468.243 kb on - strand, within MMJJ_RS08015 at 1468.244 kb on + strand, within MMJJ_RS08015 at 1468.307 kb on - strand, within MMJJ_RS08015 at 1468.308 kb on + strand, within MMJJ_RS08015 at 1468.308 kb on + strand, within MMJJ_RS08015 at 1468.308 kb on + strand, within MMJJ_RS08015 at 1468.406 kb on - strand, within MMJJ_RS08015 at 1468.406 kb on - strand, within MMJJ_RS08015 at 1468.407 kb on + strand, within MMJJ_RS08015 at 1468.407 kb on + strand, within MMJJ_RS08015 at 1468.423 kb on - strand, within MMJJ_RS08015 at 1468.424 kb on + strand, within MMJJ_RS08015 at 1468.424 kb on + strand, within MMJJ_RS08015 at 1468.457 kb on + strand, within MMJJ_RS08015 at 1468.469 kb on + strand, within MMJJ_RS08015 at 1468.480 kb on - strand, within MMJJ_RS08015 at 1468.480 kb on - strand, within MMJJ_RS08015 at 1468.510 kb on - strand, within MMJJ_RS08015 at 1468.566 kb on - strand at 1468.601 kb on + strand at 1468.611 kb on + strand at 1468.615 kb on + strand at 1468.636 kb on - strand at 1468.702 kb on - strand, within MMJJ_RS08020 at 1468.703 kb on + strand, within MMJJ_RS08020 at 1468.703 kb on + strand, within MMJJ_RS08020 at 1468.718 kb on + strand, within MMJJ_RS08020 at 1468.718 kb on + strand, within MMJJ_RS08020 at 1468.719 kb on - strand, within MMJJ_RS08020 at 1468.719 kb on - strand, within MMJJ_RS08020 at 1468.719 kb on - strand, within MMJJ_RS08020 at 1468.719 kb on - strand, within MMJJ_RS08020 at 1468.720 kb on + strand, within MMJJ_RS08020 at 1468.720 kb on + strand, within MMJJ_RS08020 at 1468.734 kb on - strand, within MMJJ_RS08020 at 1468.734 kb on - strand, within MMJJ_RS08020 at 1469.025 kb on - strand, within MMJJ_RS08020 at 1469.026 kb on + strand, within MMJJ_RS08020 at 1469.030 kb on - strand, within MMJJ_RS08020 at 1469.031 kb on + strand, within MMJJ_RS08020 at 1469.031 kb on + strand, within MMJJ_RS08020 at 1469.031 kb on + strand, within MMJJ_RS08020 at 1469.054 kb on - strand, within MMJJ_RS08020 at 1469.054 kb on - strand, within MMJJ_RS08020 at 1469.058 kb on - strand, within MMJJ_RS08020 at 1469.058 kb on - strand, within MMJJ_RS08020 at 1469.094 kb on + strand, within MMJJ_RS08020 at 1469.120 kb on - strand, within MMJJ_RS08020 at 1469.192 kb on - strand, within MMJJ_RS08020 at 1469.257 kb on - strand, within MMJJ_RS08020 at 1469.258 kb on + strand, within MMJJ_RS08020 at 1469.397 kb on - strand, within MMJJ_RS08020 at 1469.447 kb on + strand, within MMJJ_RS08020 at 1469.449 kb on - strand, within MMJJ_RS08020 at 1469.484 kb on + strand, within MMJJ_RS08020 at 1469.489 kb on - strand, within MMJJ_RS08020 at 1469.549 kb on - strand at 1469.549 kb on - strand at 1469.550 kb on + strand at 1469.642 kb on + strand at 1469.682 kb on + strand at 1469.689 kb on - strand at 1469.689 kb on - strand at 1469.690 kb on + strand at 1469.694 kb on - strand at 1469.695 kb on + strand at 1469.696 kb on - strand at 1469.696 kb on - strand at 1469.696 kb on - strand at 1469.697 kb on + strand at 1469.702 kb on + strand at 1469.706 kb on - strand at 1469.706 kb on - strand at 1469.706 kb on - strand at 1469.706 kb on - strand at 1469.707 kb on + strand at 1469.710 kb on - strand at 1469.710 kb on - strand at 1469.710 kb on - strand at 1469.710 kb on - strand at 1469.711 kb on + strand at 1469.711 kb on + strand at 1469.711 kb on + strand at 1469.711 kb on + strand at 1469.711 kb on + strand at 1469.711 kb on + strand at 1469.712 kb on - strand at 1469.713 kb on + strand at 1469.713 kb on + strand at 1469.713 kb on + strand at 1469.835 kb on + strand, within MMJJ_RS08025 at 1469.835 kb on + strand, within MMJJ_RS08025 at 1469.912 kb on - strand, within MMJJ_RS08025 at 1469.913 kb on + strand, within MMJJ_RS08025 at 1469.974 kb on - strand, within MMJJ_RS08025 at 1469.975 kb on + strand, within MMJJ_RS08025 at 1469.987 kb on + strand, within MMJJ_RS08025 at 1470.020 kb on - strand at 1470.033 kb on - strand at 1470.055 kb on + strand at 1470.060 kb on - strand at 1470.063 kb on - strand at 1470.064 kb on + strand at 1470.068 kb on + strand at 1470.161 kb on - strand at 1470.164 kb on - strand at 1470.243 kb on - strand, within MMJJ_RS08030 at 1470.362 kb on + strand, within MMJJ_RS08030 at 1470.391 kb on - strand, within MMJJ_RS08030 at 1470.409 kb on - strand, within MMJJ_RS08030 at 1470.409 kb on - strand, within MMJJ_RS08030 at 1470.472 kb on + strand, within MMJJ_RS08030 at 1470.473 kb on - strand, within MMJJ_RS08030 at 1470.539 kb on - strand, within MMJJ_RS08030 at 1470.540 kb on + strand, within MMJJ_RS08030 at 1470.545 kb on - strand, within MMJJ_RS08030 at 1470.570 kb on - strand, within MMJJ_RS08030 at 1470.571 kb on + strand, within MMJJ_RS08030 at 1470.581 kb on + strand, within MMJJ_RS08030 at 1470.608 kb on - strand, within MMJJ_RS08030 at 1470.609 kb on + strand, within MMJJ_RS08030 at 1470.614 kb on - strand, within MMJJ_RS08030 at 1470.615 kb on + strand, within MMJJ_RS08030 at 1470.618 kb on - strand, within MMJJ_RS08030 at 1470.618 kb on - strand, within MMJJ_RS08030 at 1470.619 kb on + strand, within MMJJ_RS08030 at 1470.625 kb on + strand, within MMJJ_RS08030 at 1470.630 kb on + strand, within MMJJ_RS08030 at 1470.630 kb on + strand, within MMJJ_RS08030 at 1470.635 kb on - strand, within MMJJ_RS08030 at 1470.643 kb on - strand, within MMJJ_RS08030 at 1470.643 kb on - strand, within MMJJ_RS08030 at 1470.644 kb on + strand, within MMJJ_RS08030 at 1470.648 kb on + strand, within MMJJ_RS08030
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE remove 1,467,621 - MMJJ_RS08010 0.47 -2.3 1,467,675 + MMJJ_RS08010 0.53 -2.3 1,467,675 + MMJJ_RS08010 0.53 +0.8 1,467,810 - MMJJ_RS08010 0.68 -2.5 1,467,837 - MMJJ_RS08010 0.71 -0.5 1,467,838 + MMJJ_RS08010 0.71 -2.3 1,467,838 + MMJJ_RS08010 0.71 -1.9 1,467,841 - MMJJ_RS08010 0.71 -1.9 1,467,843 - MMJJ_RS08010 0.71 -3.7 1,467,843 - MMJJ_RS08010 0.71 -2.7 1,467,844 + MMJJ_RS08010 0.72 -1.9 1,467,951 - MMJJ_RS08010 0.84 -2.1 1,468,039 + +0.1 1,468,138 + -2.1 1,468,205 + MMJJ_RS08015 0.20 -1.0 1,468,243 - MMJJ_RS08015 0.28 +1.5 1,468,243 - MMJJ_RS08015 0.28 +0.4 1,468,244 + MMJJ_RS08015 0.28 +1.6 1,468,307 - MMJJ_RS08015 0.42 +0.3 1,468,308 + MMJJ_RS08015 0.42 -2.5 1,468,308 + MMJJ_RS08015 0.42 +1.3 1,468,308 + MMJJ_RS08015 0.42 -1.7 1,468,406 - MMJJ_RS08015 0.64 +2.2 1,468,406 - MMJJ_RS08015 0.64 +0.1 1,468,407 + MMJJ_RS08015 0.64 -0.1 1,468,407 + MMJJ_RS08015 0.64 +1.7 1,468,423 - MMJJ_RS08015 0.68 -2.2 1,468,424 + MMJJ_RS08015 0.68 -1.8 1,468,424 + MMJJ_RS08015 0.68 -2.0 1,468,457 + MMJJ_RS08015 0.76 +1.8 1,468,469 + MMJJ_RS08015 0.78 -2.5 1,468,480 - MMJJ_RS08015 0.81 +1.7 1,468,480 - MMJJ_RS08015 0.81 +1.5 1,468,510 - MMJJ_RS08015 0.87 -0.9 1,468,566 - +1.4 1,468,601 + +1.2 1,468,611 + -2.4 1,468,615 + -3.4 1,468,636 - -1.9 1,468,702 - MMJJ_RS08020 0.10 -2.5 1,468,703 + MMJJ_RS08020 0.10 -3.9 1,468,703 + MMJJ_RS08020 0.10 -5.3 1,468,718 + MMJJ_RS08020 0.12 -3.7 1,468,718 + MMJJ_RS08020 0.12 -3.1 1,468,719 - MMJJ_RS08020 0.12 +0.2 1,468,719 - MMJJ_RS08020 0.12 -2.3 1,468,719 - MMJJ_RS08020 0.12 -2.9 1,468,719 - MMJJ_RS08020 0.12 -3.6 1,468,720 + MMJJ_RS08020 0.12 -0.9 1,468,720 + MMJJ_RS08020 0.12 -3.4 1,468,734 - MMJJ_RS08020 0.13 -1.9 1,468,734 - MMJJ_RS08020 0.13 -2.5 1,469,025 - MMJJ_RS08020 0.41 -3.8 1,469,026 + MMJJ_RS08020 0.41 -2.0 1,469,030 - MMJJ_RS08020 0.41 -1.9 1,469,031 + MMJJ_RS08020 0.41 -4.2 1,469,031 + MMJJ_RS08020 0.41 -4.8 1,469,031 + MMJJ_RS08020 0.41 -1.9 1,469,054 - MMJJ_RS08020 0.43 -3.9 1,469,054 - MMJJ_RS08020 0.43 -4.3 1,469,058 - MMJJ_RS08020 0.44 -4.6 1,469,058 - MMJJ_RS08020 0.44 -4.5 1,469,094 + MMJJ_RS08020 0.47 -3.8 1,469,120 - MMJJ_RS08020 0.50 -2.9 1,469,192 - MMJJ_RS08020 0.56 -4.6 1,469,257 - MMJJ_RS08020 0.63 -4.3 1,469,258 + MMJJ_RS08020 0.63 -3.7 1,469,397 - MMJJ_RS08020 0.76 -0.9 1,469,447 + MMJJ_RS08020 0.80 -0.5 1,469,449 - MMJJ_RS08020 0.81 -3.3 1,469,484 + MMJJ_RS08020 0.84 -5.6 1,469,489 - MMJJ_RS08020 0.84 -3.9 1,469,549 - -5.0 1,469,549 - -4.5 1,469,550 + -4.6 1,469,642 + -2.7 1,469,682 + -3.0 1,469,689 - -2.4 1,469,689 - -0.6 1,469,690 + -2.3 1,469,694 - -0.0 1,469,695 + -4.2 1,469,696 - +2.0 1,469,696 - +1.5 1,469,696 - +0.1 1,469,697 + -1.4 1,469,702 + -3.0 1,469,706 - +0.1 1,469,706 - -1.2 1,469,706 - -1.9 1,469,706 - -0.4 1,469,707 + -0.7 1,469,710 - +0.8 1,469,710 - -2.5 1,469,710 - -0.7 1,469,710 - -0.5 1,469,711 + +0.7 1,469,711 + -2.4 1,469,711 + -2.2 1,469,711 + -0.2 1,469,711 + -0.1 1,469,711 + -1.2 1,469,712 - -1.4 1,469,713 + -1.7 1,469,713 + -0.3 1,469,713 + +0.1 1,469,835 + MMJJ_RS08025 0.36 -1.9 1,469,835 + MMJJ_RS08025 0.36 +1.2 1,469,912 - MMJJ_RS08025 0.61 -0.5 1,469,913 + MMJJ_RS08025 0.62 +0.6 1,469,974 - MMJJ_RS08025 0.82 -3.5 1,469,975 + MMJJ_RS08025 0.82 -1.9 1,469,987 + MMJJ_RS08025 0.86 -2.1 1,470,020 - +0.8 1,470,033 - +0.3 1,470,055 + -3.0 1,470,060 - -1.7 1,470,063 - -2.0 1,470,064 + +0.3 1,470,068 + +1.0 1,470,161 - +0.9 1,470,164 - +0.4 1,470,243 - MMJJ_RS08030 0.19 -2.4 1,470,362 + MMJJ_RS08030 0.37 +1.6 1,470,391 - MMJJ_RS08030 0.41 +0.2 1,470,409 - MMJJ_RS08030 0.44 +1.0 1,470,409 - MMJJ_RS08030 0.44 -1.7 1,470,472 + MMJJ_RS08030 0.54 +1.5 1,470,473 - MMJJ_RS08030 0.54 -3.1 1,470,539 - MMJJ_RS08030 0.64 -1.4 1,470,540 + MMJJ_RS08030 0.64 +1.3 1,470,545 - MMJJ_RS08030 0.65 -1.9 1,470,570 - MMJJ_RS08030 0.69 -0.0 1,470,571 + MMJJ_RS08030 0.69 -1.4 1,470,581 + MMJJ_RS08030 0.71 -1.5 1,470,608 - MMJJ_RS08030 0.75 -2.4 1,470,609 + MMJJ_RS08030 0.75 +1.2 1,470,614 - MMJJ_RS08030 0.76 -2.5 1,470,615 + MMJJ_RS08030 0.76 -1.5 1,470,618 - MMJJ_RS08030 0.76 +1.8 1,470,618 - MMJJ_RS08030 0.76 +0.6 1,470,619 + MMJJ_RS08030 0.77 +0.8 1,470,625 + MMJJ_RS08030 0.77 +2.0 1,470,630 + MMJJ_RS08030 0.78 +1.2 1,470,630 + MMJJ_RS08030 0.78 +1.2 1,470,635 - MMJJ_RS08030 0.79 -0.5 1,470,643 - MMJJ_RS08030 0.80 -1.9 1,470,643 - MMJJ_RS08030 0.80 -2.7 1,470,644 + MMJJ_RS08030 0.80 +0.9 1,470,648 + MMJJ_RS08030 0.81 -2.7
Or see this region's nucleotide sequence