Strain Fitness in Methanococcus maripaludis JJ around MMJJ_RS07480

Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMMJJ_RS07475 and MMJJ_RS07480 are separated by 83 nucleotidesMMJJ_RS07480 and MMJJ_RS07485 are separated by 38 nucleotidesMMJJ_RS07485 and MMJJ_RS07490 are separated by 96 nucleotidesMMJJ_RS07490 and MMJJ_RS07495 are separated by 25 nucleotides MMJJ_RS07475: MMJJ_RS07475 - chorismate synthase, at 1,373,852 to 1,374,973 _RS07475 MMJJ_RS07480: MMJJ_RS07480 - FAD-dependent oxidoreductase, at 1,375,057 to 1,376,211 _RS07480 MMJJ_RS07485: MMJJ_RS07485 - DedA family protein, at 1,376,250 to 1,376,723 _RS07485 MMJJ_RS07490: MMJJ_RS07490 - HypC/HybG/HupF family hydrogenase formation chaperone, at 1,376,820 to 1,377,086 _RS07490 MMJJ_RS07495: MMJJ_RS07495 - 4Fe-4S binding protein, at 1,377,112 to 1,377,300 _RS07495 Position (kb) 1375 1376 1377Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3 4at 1374.791 kb on + strand, within MMJJ_RS07475at 1374.976 kb on - strandat 1375.056 kb on - strandat 1375.070 kb on - strandat 1375.070 kb on - strandat 1375.071 kb on + strandat 1375.071 kb on + strandat 1375.113 kb on - strandat 1375.139 kb on + strandat 1375.149 kb on - strandat 1375.170 kb on + strandat 1375.174 kb on - strand, within MMJJ_RS07480at 1375.182 kb on - strand, within MMJJ_RS07480at 1375.182 kb on - strand, within MMJJ_RS07480at 1375.182 kb on - strand, within MMJJ_RS07480at 1375.183 kb on + strand, within MMJJ_RS07480at 1375.186 kb on - strand, within MMJJ_RS07480at 1375.187 kb on + strand, within MMJJ_RS07480at 1375.187 kb on + strand, within MMJJ_RS07480at 1375.190 kb on - strand, within MMJJ_RS07480at 1375.190 kb on - strand, within MMJJ_RS07480at 1375.191 kb on + strand, within MMJJ_RS07480at 1375.191 kb on + strand, within MMJJ_RS07480at 1375.191 kb on + strand, within MMJJ_RS07480at 1375.191 kb on + strand, within MMJJ_RS07480at 1375.218 kb on + strand, within MMJJ_RS07480at 1375.229 kb on - strand, within MMJJ_RS07480at 1375.259 kb on - strand, within MMJJ_RS07480at 1375.259 kb on - strand, within MMJJ_RS07480at 1375.267 kb on + strand, within MMJJ_RS07480at 1375.268 kb on - strand, within MMJJ_RS07480at 1375.268 kb on - strand, within MMJJ_RS07480at 1375.268 kb on - strand, within MMJJ_RS07480at 1375.268 kb on - strand, within MMJJ_RS07480at 1375.268 kb on - strand, within MMJJ_RS07480at 1375.269 kb on + strand, within MMJJ_RS07480at 1375.303 kb on + strand, within MMJJ_RS07480at 1375.391 kb on + strand, within MMJJ_RS07480at 1375.451 kb on - strand, within MMJJ_RS07480at 1375.486 kb on - strand, within MMJJ_RS07480at 1375.487 kb on + strand, within MMJJ_RS07480at 1375.518 kb on + strand, within MMJJ_RS07480at 1375.546 kb on - strand, within MMJJ_RS07480at 1375.546 kb on - strand, within MMJJ_RS07480at 1375.564 kb on + strand, within MMJJ_RS07480at 1375.580 kb on - strand, within MMJJ_RS07480at 1375.580 kb on - strand, within MMJJ_RS07480at 1375.580 kb on - strand, within MMJJ_RS07480at 1375.581 kb on + strand, within MMJJ_RS07480at 1375.581 kb on + strand, within MMJJ_RS07480at 1375.588 kb on - strand, within MMJJ_RS07480at 1375.640 kb on + strand, within MMJJ_RS07480at 1375.646 kb on - strand, within MMJJ_RS07480at 1375.646 kb on - strand, within MMJJ_RS07480at 1375.647 kb on + strand, within MMJJ_RS07480at 1375.677 kb on - strand, within MMJJ_RS07480at 1375.677 kb on - strand, within MMJJ_RS07480at 1375.726 kb on + strand, within MMJJ_RS07480at 1375.769 kb on + strand, within MMJJ_RS07480at 1375.830 kb on - strand, within MMJJ_RS07480at 1375.830 kb on - strand, within MMJJ_RS07480at 1375.830 kb on - strand, within MMJJ_RS07480at 1375.830 kb on - strand, within MMJJ_RS07480at 1375.831 kb on + strand, within MMJJ_RS07480at 1375.831 kb on + strand, within MMJJ_RS07480at 1375.890 kb on - strand, within MMJJ_RS07480at 1375.890 kb on - strand, within MMJJ_RS07480at 1375.891 kb on + strand, within MMJJ_RS07480at 1375.901 kb on - strand, within MMJJ_RS07480at 1375.950 kb on - strand, within MMJJ_RS07480at 1375.951 kb on + strand, within MMJJ_RS07480at 1375.954 kb on + strand, within MMJJ_RS07480at 1375.954 kb on + strand, within MMJJ_RS07480at 1375.956 kb on + strandat 1376.018 kb on + strand, within MMJJ_RS07480at 1376.053 kb on + strand, within MMJJ_RS07480at 1376.059 kb on - strand, within MMJJ_RS07480at 1376.059 kb on - strand, within MMJJ_RS07480at 1376.101 kb on + strandat 1376.134 kb on - strandat 1376.135 kb on + strandat 1376.138 kb on - strandat 1376.155 kb on - strandat 1376.210 kb on + strandat 1376.221 kb on + strandat 1376.246 kb on - strandat 1376.247 kb on + strandat 1376.247 kb on + strandat 1376.247 kb on + strandat 1376.247 kb on + strandat 1376.285 kb on - strandat 1376.286 kb on + strandat 1376.286 kb on + strandat 1376.286 kb on + strandat 1376.289 kb on - strandat 1376.290 kb on + strandat 1376.397 kb on - strand, within MMJJ_RS07485at 1376.397 kb on - strand, within MMJJ_RS07485at 1376.398 kb on + strand, within MMJJ_RS07485at 1376.398 kb on + strand, within MMJJ_RS07485at 1376.401 kb on - strand, within MMJJ_RS07485at 1376.401 kb on - strand, within MMJJ_RS07485at 1376.401 kb on - strand, within MMJJ_RS07485at 1376.401 kb on - strand, within MMJJ_RS07485at 1376.402 kb on + strand, within MMJJ_RS07485at 1376.404 kb on - strand, within MMJJ_RS07485at 1376.405 kb on + strand, within MMJJ_RS07485at 1376.405 kb on + strand, within MMJJ_RS07485at 1376.427 kb on + strand, within MMJJ_RS07485at 1376.443 kb on - strand, within MMJJ_RS07485at 1376.444 kb on + strand, within MMJJ_RS07485at 1376.448 kb on - strand, within MMJJ_RS07485at 1376.449 kb on + strand, within MMJJ_RS07485at 1376.449 kb on + strand, within MMJJ_RS07485at 1376.453 kb on - strand, within MMJJ_RS07485at 1376.454 kb on + strand, within MMJJ_RS07485at 1376.455 kb on - strand, within MMJJ_RS07485at 1376.480 kb on + strand, within MMJJ_RS07485at 1376.486 kb on + strand, within MMJJ_RS07485at 1376.534 kb on - strand, within MMJJ_RS07485at 1376.559 kb on + strand, within MMJJ_RS07485at 1376.652 kb on + strand, within MMJJ_RS07485at 1376.665 kb on - strand, within MMJJ_RS07485at 1376.665 kb on - strand, within MMJJ_RS07485at 1376.684 kb on + strandat 1376.710 kb on - strandat 1376.754 kb on - strandat 1376.757 kb on + strandat 1376.760 kb on - strandat 1376.761 kb on + strandat 1376.761 kb on + strandat 1376.765 kb on - strandat 1376.765 kb on - strandat 1376.797 kb on - strandat 1376.797 kb on - strandat 1376.797 kb on - strandat 1376.797 kb on - strandat 1376.802 kb on - strandat 1376.953 kb on + strandat 1376.953 kb on + strand, within MMJJ_RS07490at 1376.953 kb on + strand, within MMJJ_RS07490at 1377.043 kb on - strand, within MMJJ_RS07490at 1377.043 kb on - strand, within MMJJ_RS07490at 1377.044 kb on + strand, within MMJJ_RS07490at 1377.085 kb on + strandat 1377.113 kb on - strandat 1377.148 kb on - strand, within MMJJ_RS07495at 1377.166 kb on + strand, within MMJJ_RS07495at 1377.169 kb on - strand, within MMJJ_RS07495at 1377.170 kb on + strand, within MMJJ_RS07495at 1377.170 kb on + strand, within MMJJ_RS07495at 1377.170 kb on + strand, within MMJJ_RS07495at 1377.170 kb on + strand, within MMJJ_RS07495at 1377.170 kb on + strand, within MMJJ_RS07495at 1377.174 kb on - strand, within MMJJ_RS07495at 1377.175 kb on + strand, within MMJJ_RS07495at 1377.175 kb on + strand, within MMJJ_RS07495at 1377.199 kb on - strand, within MMJJ_RS07495at 1377.199 kb on - strand, within MMJJ_RS07495at 1377.199 kb on - strand, within MMJJ_RS07495at 1377.200 kb on + strand, within MMJJ_RS07495

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Per-strain Table

Position Strand Gene LocusTag Fraction Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE
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1,374,791 + MMJJ_RS07475 0.84 -2.5
1,374,976 - -1.3
1,375,056 - -1.8
1,375,070 - -0.9
1,375,070 - +2.6
1,375,071 + -2.3
1,375,071 + +2.0
1,375,113 - -2.1
1,375,139 + -2.3
1,375,149 - +0.1
1,375,170 + -1.4
1,375,174 - MMJJ_RS07480 0.10 +1.5
1,375,182 - MMJJ_RS07480 0.11 -3.2
1,375,182 - MMJJ_RS07480 0.11 -0.3
1,375,182 - MMJJ_RS07480 0.11 -1.5
1,375,183 + MMJJ_RS07480 0.11 +1.7
1,375,186 - MMJJ_RS07480 0.11 -1.1
1,375,187 + MMJJ_RS07480 0.11 -2.1
1,375,187 + MMJJ_RS07480 0.11 +0.9
1,375,190 - MMJJ_RS07480 0.12 -0.6
1,375,190 - MMJJ_RS07480 0.12 -1.6
1,375,191 + MMJJ_RS07480 0.12 +2.1
1,375,191 + MMJJ_RS07480 0.12 -0.2
1,375,191 + MMJJ_RS07480 0.12 +1.3
1,375,191 + MMJJ_RS07480 0.12 +1.7
1,375,218 + MMJJ_RS07480 0.14 +1.6
1,375,229 - MMJJ_RS07480 0.15 -1.5
1,375,259 - MMJJ_RS07480 0.17 -1.0
1,375,259 - MMJJ_RS07480 0.17 -1.0
1,375,267 + MMJJ_RS07480 0.18 -3.8
1,375,268 - MMJJ_RS07480 0.18 -1.9
1,375,268 - MMJJ_RS07480 0.18 +1.7
1,375,268 - MMJJ_RS07480 0.18 +3.3
1,375,268 - MMJJ_RS07480 0.18 +1.2
1,375,268 - MMJJ_RS07480 0.18 -1.2
1,375,269 + MMJJ_RS07480 0.18 -0.3
1,375,303 + MMJJ_RS07480 0.21 +2.0
1,375,391 + MMJJ_RS07480 0.29 -0.7
1,375,451 - MMJJ_RS07480 0.34 -1.7
1,375,486 - MMJJ_RS07480 0.37 +1.6
1,375,487 + MMJJ_RS07480 0.37 +1.4
1,375,518 + MMJJ_RS07480 0.40 +1.4
1,375,546 - MMJJ_RS07480 0.42 -0.6
1,375,546 - MMJJ_RS07480 0.42 +0.8
1,375,564 + MMJJ_RS07480 0.44 +0.9
1,375,580 - MMJJ_RS07480 0.45 +0.6
1,375,580 - MMJJ_RS07480 0.45 -1.8
1,375,580 - MMJJ_RS07480 0.45 +1.5
1,375,581 + MMJJ_RS07480 0.45 +1.2
1,375,581 + MMJJ_RS07480 0.45 +1.0
1,375,588 - MMJJ_RS07480 0.46 +1.7
1,375,640 + MMJJ_RS07480 0.50 -0.8
1,375,646 - MMJJ_RS07480 0.51 +0.5
1,375,646 - MMJJ_RS07480 0.51 -2.2
1,375,647 + MMJJ_RS07480 0.51 +1.8
1,375,677 - MMJJ_RS07480 0.54 +0.9
1,375,677 - MMJJ_RS07480 0.54 +2.0
1,375,726 + MMJJ_RS07480 0.58 +1.3
1,375,769 + MMJJ_RS07480 0.62 -0.2
1,375,830 - MMJJ_RS07480 0.67 -1.9
1,375,830 - MMJJ_RS07480 0.67 +4.5
1,375,830 - MMJJ_RS07480 0.67 +0.5
1,375,830 - MMJJ_RS07480 0.67 +1.3
1,375,831 + MMJJ_RS07480 0.67 +1.9
1,375,831 + MMJJ_RS07480 0.67 +0.5
1,375,890 - MMJJ_RS07480 0.72 +0.5
1,375,890 - MMJJ_RS07480 0.72 +2.1
1,375,891 + MMJJ_RS07480 0.72 -1.5
1,375,901 - MMJJ_RS07480 0.73 -0.7
1,375,950 - MMJJ_RS07480 0.77 +0.9
1,375,951 + MMJJ_RS07480 0.77 +1.1
1,375,954 + MMJJ_RS07480 0.78 +2.2
1,375,954 + MMJJ_RS07480 0.78 -3.0
1,375,956 + -0.7
1,376,018 + MMJJ_RS07480 0.83 +1.1
1,376,053 + MMJJ_RS07480 0.86 -2.2
1,376,059 - MMJJ_RS07480 0.87 +1.2
1,376,059 - MMJJ_RS07480 0.87 -1.9
1,376,101 + -2.2
1,376,134 - +1.2
1,376,135 + -1.6
1,376,138 - +2.3
1,376,155 - +0.1
1,376,210 + +1.3
1,376,221 + -0.8
1,376,246 - -0.5
1,376,247 + -3.2
1,376,247 + +0.3
1,376,247 + -0.8
1,376,247 + +0.3
1,376,285 - +0.9
1,376,286 + +0.8
1,376,286 + -2.9
1,376,286 + -0.1
1,376,289 - -1.7
1,376,290 + +0.4
1,376,397 - MMJJ_RS07485 0.31 +0.4
1,376,397 - MMJJ_RS07485 0.31 -0.7
1,376,398 + MMJJ_RS07485 0.31 +1.5
1,376,398 + MMJJ_RS07485 0.31 +0.6
1,376,401 - MMJJ_RS07485 0.32 +0.5
1,376,401 - MMJJ_RS07485 0.32 +1.1
1,376,401 - MMJJ_RS07485 0.32 -1.9
1,376,401 - MMJJ_RS07485 0.32 +0.2
1,376,402 + MMJJ_RS07485 0.32 -0.7
1,376,404 - MMJJ_RS07485 0.32 -2.9
1,376,405 + MMJJ_RS07485 0.33 -1.0
1,376,405 + MMJJ_RS07485 0.33 -1.6
1,376,427 + MMJJ_RS07485 0.37 +0.8
1,376,443 - MMJJ_RS07485 0.41 -1.7
1,376,444 + MMJJ_RS07485 0.41 +1.0
1,376,448 - MMJJ_RS07485 0.42 -0.7
1,376,449 + MMJJ_RS07485 0.42 +1.3
1,376,449 + MMJJ_RS07485 0.42 -2.5
1,376,453 - MMJJ_RS07485 0.43 -2.7
1,376,454 + MMJJ_RS07485 0.43 -2.7
1,376,455 - MMJJ_RS07485 0.43 -0.7
1,376,480 + MMJJ_RS07485 0.49 -1.0
1,376,486 + MMJJ_RS07485 0.50 +0.2
1,376,534 - MMJJ_RS07485 0.60 +1.9
1,376,559 + MMJJ_RS07485 0.65 -0.7
1,376,652 + MMJJ_RS07485 0.85 +0.9
1,376,665 - MMJJ_RS07485 0.88 -0.8
1,376,665 - MMJJ_RS07485 0.88 -1.3
1,376,684 + -2.5
1,376,710 - +1.4
1,376,754 - +0.7
1,376,757 + +1.0
1,376,760 - -3.5
1,376,761 + -3.9
1,376,761 + -2.1
1,376,765 - +0.1
1,376,765 - -3.8
1,376,797 - -1.4
1,376,797 - -0.7
1,376,797 - -1.3
1,376,797 - -2.3
1,376,802 - -0.7
1,376,953 + -1.7
1,376,953 + MMJJ_RS07490 0.50 -1.3
1,376,953 + MMJJ_RS07490 0.50 -2.5
1,377,043 - MMJJ_RS07490 0.84 -2.7
1,377,043 - MMJJ_RS07490 0.84 +0.5
1,377,044 + MMJJ_RS07490 0.84 -0.8
1,377,085 + +1.1
1,377,113 - -0.3
1,377,148 - MMJJ_RS07495 0.19 +0.4
1,377,166 + MMJJ_RS07495 0.29 +0.5
1,377,169 - MMJJ_RS07495 0.30 +0.2
1,377,170 + MMJJ_RS07495 0.31 +1.0
1,377,170 + MMJJ_RS07495 0.31 -1.2
1,377,170 + MMJJ_RS07495 0.31 -1.1
1,377,170 + MMJJ_RS07495 0.31 -3.0
1,377,170 + MMJJ_RS07495 0.31 +1.4
1,377,174 - MMJJ_RS07495 0.33 +1.2
1,377,175 + MMJJ_RS07495 0.33 +1.4
1,377,175 + MMJJ_RS07495 0.33 +1.7
1,377,199 - MMJJ_RS07495 0.46 +1.0
1,377,199 - MMJJ_RS07495 0.46 -1.5
1,377,199 - MMJJ_RS07495 0.46 +1.8
1,377,200 + MMJJ_RS07495 0.47 +1.0

Or see this region's nucleotide sequence