Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS04795 and MMJJ_RS04800 are separated by 46 nucleotides MMJJ_RS04800 and MMJJ_RS04805 are separated by 23 nucleotides MMJJ_RS04805 and MMJJ_RS04810 are separated by 133 nucleotides MMJJ_RS04810 and MMJJ_RS04815 are separated by 168 nucleotides
MMJJ_RS04795: MMJJ_RS04795 - MBL fold metallo-hydrolase, at 899,071 to 899,688
_RS04795
MMJJ_RS04800: MMJJ_RS04800 - 2-phospho-L-lactate guanylyltransferase, at 899,735 to 900,403
_RS04800
MMJJ_RS04805: MMJJ_RS04805 - N-acetyl-gamma-glutamyl-phosphate reductase, at 900,427 to 901,458
_RS04805
MMJJ_RS04810: MMJJ_RS04810 - nitroreductase family protein, at 901,592 to 902,113
_RS04810
MMJJ_RS04815: MMJJ_RS04815 - TIM barrel protein, at 902,282 to 902,938
_RS04815
Position (kb) 900
901
902 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 899.441 kb on - strand, within MMJJ_RS04795 at 899.441 kb on - strand, within MMJJ_RS04795 at 899.441 kb on - strand, within MMJJ_RS04795 at 899.442 kb on + strand, within MMJJ_RS04795 at 899.442 kb on + strand, within MMJJ_RS04795 at 899.442 kb on + strand, within MMJJ_RS04795 at 899.442 kb on + strand, within MMJJ_RS04795 at 899.442 kb on + strand, within MMJJ_RS04795 at 899.442 kb on + strand, within MMJJ_RS04795 at 899.472 kb on + strand, within MMJJ_RS04795 at 899.477 kb on - strand, within MMJJ_RS04795 at 899.478 kb on + strand, within MMJJ_RS04795 at 899.478 kb on + strand, within MMJJ_RS04795 at 899.480 kb on - strand, within MMJJ_RS04795 at 899.481 kb on + strand, within MMJJ_RS04795 at 899.604 kb on - strand, within MMJJ_RS04795 at 899.624 kb on - strand, within MMJJ_RS04795 at 899.625 kb on + strand, within MMJJ_RS04795 at 899.625 kb on + strand, within MMJJ_RS04795 at 899.626 kb on - strand, within MMJJ_RS04795 at 899.654 kb on - strand at 899.678 kb on - strand at 899.679 kb on + strand at 899.682 kb on - strand at 899.682 kb on - strand at 899.682 kb on - strand at 899.682 kb on - strand at 899.682 kb on - strand at 899.682 kb on - strand at 899.683 kb on + strand at 899.683 kb on + strand at 899.717 kb on + strand at 899.749 kb on + strand at 899.775 kb on - strand at 899.838 kb on - strand, within MMJJ_RS04800 at 899.838 kb on - strand, within MMJJ_RS04800 at 899.838 kb on - strand, within MMJJ_RS04800 at 899.850 kb on + strand, within MMJJ_RS04800 at 899.883 kb on + strand, within MMJJ_RS04800 at 899.963 kb on + strand, within MMJJ_RS04800 at 900.073 kb on - strand, within MMJJ_RS04800 at 900.253 kb on - strand, within MMJJ_RS04800 at 900.254 kb on + strand, within MMJJ_RS04800 at 900.254 kb on + strand, within MMJJ_RS04800 at 900.261 kb on - strand, within MMJJ_RS04800 at 900.262 kb on + strand, within MMJJ_RS04800 at 900.267 kb on - strand, within MMJJ_RS04800 at 900.268 kb on + strand, within MMJJ_RS04800 at 900.268 kb on + strand, within MMJJ_RS04800 at 900.286 kb on + strand at 900.324 kb on + strand, within MMJJ_RS04800 at 900.324 kb on + strand, within MMJJ_RS04800 at 900.330 kb on + strand, within MMJJ_RS04800 at 900.330 kb on + strand at 900.429 kb on + strand at 900.483 kb on - strand at 900.874 kb on - strand, within MMJJ_RS04805 at 901.178 kb on + strand, within MMJJ_RS04805 at 901.293 kb on - strand, within MMJJ_RS04805 at 901.499 kb on + strand at 901.526 kb on - strand at 901.527 kb on + strand at 901.533 kb on - strand at 901.563 kb on + strand at 901.599 kb on - strand at 901.620 kb on - strand at 901.625 kb on - strand at 901.686 kb on + strand, within MMJJ_RS04810 at 901.754 kb on - strand, within MMJJ_RS04810 at 901.755 kb on + strand, within MMJJ_RS04810 at 901.761 kb on - strand, within MMJJ_RS04810 at 901.762 kb on + strand, within MMJJ_RS04810 at 901.776 kb on + strand, within MMJJ_RS04810 at 901.805 kb on - strand, within MMJJ_RS04810 at 901.805 kb on - strand at 901.805 kb on - strand, within MMJJ_RS04810 at 901.805 kb on - strand, within MMJJ_RS04810 at 901.808 kb on - strand, within MMJJ_RS04810 at 901.809 kb on + strand, within MMJJ_RS04810 at 901.811 kb on - strand, within MMJJ_RS04810 at 901.811 kb on - strand, within MMJJ_RS04810 at 901.889 kb on - strand, within MMJJ_RS04810 at 901.889 kb on - strand, within MMJJ_RS04810 at 901.889 kb on - strand, within MMJJ_RS04810 at 901.895 kb on - strand, within MMJJ_RS04810 at 901.896 kb on + strand, within MMJJ_RS04810 at 901.896 kb on + strand, within MMJJ_RS04810 at 901.977 kb on + strand, within MMJJ_RS04810 at 902.048 kb on - strand, within MMJJ_RS04810 at 902.076 kb on - strand at 902.076 kb on - strand at 902.077 kb on + strand at 902.102 kb on - strand at 902.113 kb on - strand at 902.113 kb on - strand at 902.114 kb on + strand at 902.114 kb on + strand at 902.115 kb on - strand at 902.115 kb on - strand at 902.143 kb on + strand at 902.147 kb on + strand at 902.170 kb on - strand at 902.171 kb on + strand at 902.171 kb on + strand at 902.172 kb on - strand at 902.172 kb on - strand at 902.172 kb on - strand at 902.196 kb on - strand at 902.198 kb on - strand at 902.198 kb on - strand at 902.199 kb on + strand at 902.199 kb on + strand at 902.199 kb on + strand at 902.199 kb on + strand at 902.247 kb on + strand at 902.281 kb on - strand at 902.282 kb on + strand at 902.287 kb on + strand at 902.332 kb on - strand at 902.413 kb on + strand, within MMJJ_RS04815
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE remove 899,441 - MMJJ_RS04795 0.60 +1.1 899,441 - MMJJ_RS04795 0.60 -1.3 899,441 - MMJJ_RS04795 0.60 -0.9 899,442 + MMJJ_RS04795 0.60 +1.5 899,442 + MMJJ_RS04795 0.60 +0.9 899,442 + MMJJ_RS04795 0.60 -1.7 899,442 + MMJJ_RS04795 0.60 -1.1 899,442 + MMJJ_RS04795 0.60 +1.0 899,442 + MMJJ_RS04795 0.60 +1.6 899,472 + MMJJ_RS04795 0.65 +1.6 899,477 - MMJJ_RS04795 0.66 -1.6 899,478 + MMJJ_RS04795 0.66 +0.5 899,478 + MMJJ_RS04795 0.66 +1.6 899,480 - MMJJ_RS04795 0.66 -0.9 899,481 + MMJJ_RS04795 0.66 +1.5 899,604 - MMJJ_RS04795 0.86 +0.8 899,624 - MMJJ_RS04795 0.89 +1.4 899,625 + MMJJ_RS04795 0.90 -2.8 899,625 + MMJJ_RS04795 0.90 -1.1 899,626 - MMJJ_RS04795 0.90 +0.4 899,654 - -1.4 899,678 - -2.4 899,679 + -1.5 899,682 - +0.9 899,682 - +0.1 899,682 - -1.5 899,682 - -2.3 899,682 - +0.9 899,682 - -1.7 899,683 + +0.9 899,683 + -3.0 899,717 + -4.4 899,749 + -1.5 899,775 - +0.1 899,838 - MMJJ_RS04800 0.15 -2.9 899,838 - MMJJ_RS04800 0.15 -3.9 899,838 - MMJJ_RS04800 0.15 -1.5 899,850 + MMJJ_RS04800 0.17 -3.4 899,883 + MMJJ_RS04800 0.22 -1.9 899,963 + MMJJ_RS04800 0.34 -1.5 900,073 - MMJJ_RS04800 0.51 -3.4 900,253 - MMJJ_RS04800 0.77 -2.7 900,254 + MMJJ_RS04800 0.78 -1.5 900,254 + MMJJ_RS04800 0.78 -2.7 900,261 - MMJJ_RS04800 0.79 -0.9 900,262 + MMJJ_RS04800 0.79 -1.5 900,267 - MMJJ_RS04800 0.80 -2.2 900,268 + MMJJ_RS04800 0.80 -3.1 900,268 + MMJJ_RS04800 0.80 -1.5 900,286 + +0.1 900,324 + MMJJ_RS04800 0.88 -1.2 900,324 + MMJJ_RS04800 0.88 -3.1 900,330 + MMJJ_RS04800 0.89 -3.5 900,330 + +0.1 900,429 + +1.3 900,483 - -1.5 900,874 - MMJJ_RS04805 0.43 +0.1 901,178 + MMJJ_RS04805 0.73 -1.9 901,293 - MMJJ_RS04805 0.84 -3.4 901,499 + -1.9 901,526 - +1.3 901,527 + +1.5 901,533 - +1.9 901,563 + +0.4 901,599 - -3.2 901,620 - -1.1 901,625 - -2.7 901,686 + MMJJ_RS04810 0.18 -2.6 901,754 - MMJJ_RS04810 0.31 +1.4 901,755 + MMJJ_RS04810 0.31 -2.6 901,761 - MMJJ_RS04810 0.32 -2.6 901,762 + MMJJ_RS04810 0.33 -0.5 901,776 + MMJJ_RS04810 0.35 +2.1 901,805 - MMJJ_RS04810 0.41 +0.6 901,805 - -1.2 901,805 - MMJJ_RS04810 0.41 +1.1 901,805 - MMJJ_RS04810 0.41 +0.1 901,808 - MMJJ_RS04810 0.41 -2.6 901,809 + MMJJ_RS04810 0.42 -1.4 901,811 - MMJJ_RS04810 0.42 +2.2 901,811 - MMJJ_RS04810 0.42 -2.0 901,889 - MMJJ_RS04810 0.57 +0.1 901,889 - MMJJ_RS04810 0.57 -1.4 901,889 - MMJJ_RS04810 0.57 +1.6 901,895 - MMJJ_RS04810 0.58 +1.0 901,896 + MMJJ_RS04810 0.58 +1.4 901,896 + MMJJ_RS04810 0.58 +2.2 901,977 + MMJJ_RS04810 0.74 -0.2 902,048 - MMJJ_RS04810 0.87 -2.0 902,076 - +0.2 902,076 - -3.9 902,077 + +2.5 902,102 - +1.0 902,113 - +1.1 902,113 - +0.6 902,114 + +0.7 902,114 + +1.0 902,115 - +1.4 902,115 - -1.7 902,143 + +1.2 902,147 + +2.2 902,170 - -0.3 902,171 + -1.3 902,171 + +1.2 902,172 - +0.9 902,172 - +1.0 902,172 - -2.0 902,196 - -1.3 902,198 - -0.7 902,198 - -0.0 902,199 + +1.7 902,199 + +0.4 902,199 + +0.1 902,199 + +1.5 902,247 + +0.5 902,281 - -2.1 902,282 + +1.3 902,287 + +0.8 902,332 - -3.1 902,413 + MMJJ_RS04815 0.20 +0.1
Or see this region's nucleotide sequence