Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS04785 and MMJJ_RS04790 overlap by 20 nucleotides MMJJ_RS04790 and MMJJ_RS04795 are separated by 12 nucleotides MMJJ_RS04795 and MMJJ_RS04800 are separated by 46 nucleotides MMJJ_RS04800 and MMJJ_RS04805 are separated by 23 nucleotides
MMJJ_RS04785: MMJJ_RS04785 - OB-fold nucleic acid binding domain-containing protein, at 897,966 to 898,352
_RS04785
MMJJ_RS04790: MMJJ_RS04790 - biotin--[acetyl-CoA-carboxylase] ligase, at 898,333 to 899,058
_RS04790
MMJJ_RS04795: MMJJ_RS04795 - MBL fold metallo-hydrolase, at 899,071 to 899,688
_RS04795
MMJJ_RS04800: MMJJ_RS04800 - 2-phospho-L-lactate guanylyltransferase, at 899,735 to 900,403
_RS04800
MMJJ_RS04805: MMJJ_RS04805 - N-acetyl-gamma-glutamyl-phosphate reductase, at 900,427 to 901,458
_RS04805
Position (kb) 899
900 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 898.093 kb on - strand, within MMJJ_RS04785 at 898.103 kb on + strand, within MMJJ_RS04785 at 898.145 kb on - strand, within MMJJ_RS04785 at 898.145 kb on - strand, within MMJJ_RS04785 at 898.268 kb on + strand, within MMJJ_RS04785 at 898.277 kb on - strand, within MMJJ_RS04785 at 898.277 kb on - strand, within MMJJ_RS04785 at 898.277 kb on - strand, within MMJJ_RS04785 at 898.278 kb on + strand, within MMJJ_RS04785 at 898.281 kb on - strand, within MMJJ_RS04785 at 898.282 kb on + strand, within MMJJ_RS04785 at 898.282 kb on + strand, within MMJJ_RS04785 at 898.282 kb on + strand, within MMJJ_RS04785 at 898.282 kb on + strand, within MMJJ_RS04785 at 898.282 kb on + strand, within MMJJ_RS04785 at 898.282 kb on + strand, within MMJJ_RS04785 at 898.282 kb on + strand, within MMJJ_RS04785 at 898.285 kb on - strand, within MMJJ_RS04785 at 898.286 kb on + strand, within MMJJ_RS04785 at 898.286 kb on + strand, within MMJJ_RS04785 at 898.330 kb on - strand at 898.336 kb on - strand at 898.336 kb on - strand at 898.337 kb on + strand at 898.337 kb on + strand at 898.337 kb on + strand at 898.351 kb on + strand at 898.351 kb on + strand at 898.411 kb on - strand, within MMJJ_RS04790 at 898.412 kb on + strand, within MMJJ_RS04790 at 898.412 kb on + strand, within MMJJ_RS04790 at 898.485 kb on - strand, within MMJJ_RS04790 at 898.602 kb on + strand, within MMJJ_RS04790 at 898.722 kb on + strand at 898.790 kb on - strand, within MMJJ_RS04790 at 898.850 kb on - strand, within MMJJ_RS04790 at 898.851 kb on + strand, within MMJJ_RS04790 at 898.924 kb on + strand at 898.931 kb on - strand, within MMJJ_RS04790 at 899.056 kb on - strand at 899.068 kb on + strand at 899.079 kb on - strand at 899.113 kb on - strand at 899.117 kb on - strand at 899.120 kb on - strand at 899.144 kb on - strand, within MMJJ_RS04795 at 899.192 kb on - strand, within MMJJ_RS04795 at 899.192 kb on - strand, within MMJJ_RS04795 at 899.193 kb on + strand, within MMJJ_RS04795 at 899.193 kb on + strand, within MMJJ_RS04795 at 899.193 kb on + strand, within MMJJ_RS04795 at 899.197 kb on - strand, within MMJJ_RS04795 at 899.198 kb on + strand, within MMJJ_RS04795 at 899.215 kb on - strand, within MMJJ_RS04795 at 899.216 kb on + strand, within MMJJ_RS04795 at 899.216 kb on + strand, within MMJJ_RS04795 at 899.216 kb on + strand, within MMJJ_RS04795 at 899.220 kb on - strand, within MMJJ_RS04795 at 899.221 kb on + strand, within MMJJ_RS04795 at 899.306 kb on - strand, within MMJJ_RS04795 at 899.307 kb on + strand, within MMJJ_RS04795 at 899.307 kb on + strand, within MMJJ_RS04795 at 899.307 kb on + strand, within MMJJ_RS04795 at 899.341 kb on + strand, within MMJJ_RS04795 at 899.341 kb on + strand, within MMJJ_RS04795 at 899.345 kb on - strand, within MMJJ_RS04795 at 899.349 kb on - strand, within MMJJ_RS04795 at 899.397 kb on + strand, within MMJJ_RS04795 at 899.397 kb on + strand, within MMJJ_RS04795 at 899.397 kb on + strand, within MMJJ_RS04795 at 899.417 kb on - strand, within MMJJ_RS04795 at 899.441 kb on - strand, within MMJJ_RS04795 at 899.441 kb on - strand, within MMJJ_RS04795 at 899.441 kb on - strand, within MMJJ_RS04795 at 899.442 kb on + strand, within MMJJ_RS04795 at 899.442 kb on + strand, within MMJJ_RS04795 at 899.442 kb on + strand, within MMJJ_RS04795 at 899.442 kb on + strand, within MMJJ_RS04795 at 899.442 kb on + strand, within MMJJ_RS04795 at 899.442 kb on + strand, within MMJJ_RS04795 at 899.472 kb on + strand, within MMJJ_RS04795 at 899.477 kb on - strand, within MMJJ_RS04795 at 899.478 kb on + strand, within MMJJ_RS04795 at 899.478 kb on + strand, within MMJJ_RS04795 at 899.480 kb on - strand, within MMJJ_RS04795 at 899.481 kb on + strand, within MMJJ_RS04795 at 899.604 kb on - strand, within MMJJ_RS04795 at 899.624 kb on - strand, within MMJJ_RS04795 at 899.625 kb on + strand, within MMJJ_RS04795 at 899.625 kb on + strand, within MMJJ_RS04795 at 899.626 kb on - strand, within MMJJ_RS04795 at 899.654 kb on - strand at 899.678 kb on - strand at 899.679 kb on + strand at 899.682 kb on - strand at 899.682 kb on - strand at 899.682 kb on - strand at 899.682 kb on - strand at 899.682 kb on - strand at 899.682 kb on - strand at 899.683 kb on + strand at 899.683 kb on + strand at 899.717 kb on + strand at 899.749 kb on + strand at 899.775 kb on - strand at 899.838 kb on - strand, within MMJJ_RS04800 at 899.838 kb on - strand, within MMJJ_RS04800 at 899.838 kb on - strand, within MMJJ_RS04800 at 899.850 kb on + strand, within MMJJ_RS04800 at 899.883 kb on + strand, within MMJJ_RS04800 at 899.963 kb on + strand, within MMJJ_RS04800 at 900.073 kb on - strand, within MMJJ_RS04800 at 900.253 kb on - strand, within MMJJ_RS04800 at 900.254 kb on + strand, within MMJJ_RS04800 at 900.254 kb on + strand, within MMJJ_RS04800 at 900.261 kb on - strand, within MMJJ_RS04800 at 900.262 kb on + strand, within MMJJ_RS04800 at 900.267 kb on - strand, within MMJJ_RS04800 at 900.268 kb on + strand, within MMJJ_RS04800 at 900.268 kb on + strand, within MMJJ_RS04800 at 900.286 kb on + strand at 900.324 kb on + strand, within MMJJ_RS04800 at 900.324 kb on + strand, within MMJJ_RS04800 at 900.330 kb on + strand, within MMJJ_RS04800 at 900.330 kb on + strand at 900.429 kb on + strand at 900.483 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE remove 898,093 - MMJJ_RS04785 0.33 +1.1 898,103 + MMJJ_RS04785 0.35 +0.1 898,145 - MMJJ_RS04785 0.46 -1.2 898,145 - MMJJ_RS04785 0.46 -0.5 898,268 + MMJJ_RS04785 0.78 -1.5 898,277 - MMJJ_RS04785 0.80 -1.4 898,277 - MMJJ_RS04785 0.80 -2.6 898,277 - MMJJ_RS04785 0.80 -1.4 898,278 + MMJJ_RS04785 0.81 -1.3 898,281 - MMJJ_RS04785 0.81 +1.4 898,282 + MMJJ_RS04785 0.82 -0.6 898,282 + MMJJ_RS04785 0.82 -0.9 898,282 + MMJJ_RS04785 0.82 -0.9 898,282 + MMJJ_RS04785 0.82 +0.5 898,282 + MMJJ_RS04785 0.82 -1.1 898,282 + MMJJ_RS04785 0.82 +1.4 898,282 + MMJJ_RS04785 0.82 +1.2 898,285 - MMJJ_RS04785 0.82 +0.2 898,286 + MMJJ_RS04785 0.83 -1.5 898,286 + MMJJ_RS04785 0.83 +0.9 898,330 - -0.8 898,336 - -3.2 898,336 - -1.5 898,337 + -0.2 898,337 + -1.8 898,337 + +0.8 898,351 + -3.7 898,351 + -2.2 898,411 - MMJJ_RS04790 0.11 -0.9 898,412 + MMJJ_RS04790 0.11 -2.7 898,412 + MMJJ_RS04790 0.11 -2.5 898,485 - MMJJ_RS04790 0.21 -1.5 898,602 + MMJJ_RS04790 0.37 -1.9 898,722 + -0.9 898,790 - MMJJ_RS04790 0.63 -1.9 898,850 - MMJJ_RS04790 0.71 -3.4 898,851 + MMJJ_RS04790 0.71 -1.9 898,924 + -1.9 898,931 - MMJJ_RS04790 0.82 -2.1 899,056 - +1.0 899,068 + -2.7 899,079 - -0.4 899,113 - +1.9 899,117 - -2.1 899,120 - +1.2 899,144 - MMJJ_RS04795 0.12 -1.4 899,192 - MMJJ_RS04795 0.20 -1.1 899,192 - MMJJ_RS04795 0.20 -1.4 899,193 + MMJJ_RS04795 0.20 -2.5 899,193 + MMJJ_RS04795 0.20 +1.9 899,193 + MMJJ_RS04795 0.20 +1.0 899,197 - MMJJ_RS04795 0.20 +1.2 899,198 + MMJJ_RS04795 0.21 +0.7 899,215 - MMJJ_RS04795 0.23 -1.7 899,216 + MMJJ_RS04795 0.23 +0.2 899,216 + MMJJ_RS04795 0.23 -0.9 899,216 + MMJJ_RS04795 0.23 +0.9 899,220 - MMJJ_RS04795 0.24 -2.6 899,221 + MMJJ_RS04795 0.24 -0.8 899,306 - MMJJ_RS04795 0.38 -0.8 899,307 + MMJJ_RS04795 0.38 -2.8 899,307 + MMJJ_RS04795 0.38 +0.9 899,307 + MMJJ_RS04795 0.38 -2.4 899,341 + MMJJ_RS04795 0.44 +2.1 899,341 + MMJJ_RS04795 0.44 -1.9 899,345 - MMJJ_RS04795 0.44 +0.9 899,349 - MMJJ_RS04795 0.45 -1.1 899,397 + MMJJ_RS04795 0.53 -2.0 899,397 + MMJJ_RS04795 0.53 +1.6 899,397 + MMJJ_RS04795 0.53 -0.6 899,417 - MMJJ_RS04795 0.56 -1.5 899,441 - MMJJ_RS04795 0.60 +1.1 899,441 - MMJJ_RS04795 0.60 -1.3 899,441 - MMJJ_RS04795 0.60 -0.9 899,442 + MMJJ_RS04795 0.60 +1.5 899,442 + MMJJ_RS04795 0.60 +0.9 899,442 + MMJJ_RS04795 0.60 -1.7 899,442 + MMJJ_RS04795 0.60 -1.1 899,442 + MMJJ_RS04795 0.60 +1.0 899,442 + MMJJ_RS04795 0.60 +1.6 899,472 + MMJJ_RS04795 0.65 +1.6 899,477 - MMJJ_RS04795 0.66 -1.6 899,478 + MMJJ_RS04795 0.66 +0.5 899,478 + MMJJ_RS04795 0.66 +1.6 899,480 - MMJJ_RS04795 0.66 -0.9 899,481 + MMJJ_RS04795 0.66 +1.5 899,604 - MMJJ_RS04795 0.86 +0.8 899,624 - MMJJ_RS04795 0.89 +1.4 899,625 + MMJJ_RS04795 0.90 -2.8 899,625 + MMJJ_RS04795 0.90 -1.1 899,626 - MMJJ_RS04795 0.90 +0.4 899,654 - -1.4 899,678 - -2.4 899,679 + -1.5 899,682 - +0.9 899,682 - +0.1 899,682 - -1.5 899,682 - -2.3 899,682 - +0.9 899,682 - -1.7 899,683 + +0.9 899,683 + -3.0 899,717 + -4.4 899,749 + -1.5 899,775 - +0.1 899,838 - MMJJ_RS04800 0.15 -2.9 899,838 - MMJJ_RS04800 0.15 -3.9 899,838 - MMJJ_RS04800 0.15 -1.5 899,850 + MMJJ_RS04800 0.17 -3.4 899,883 + MMJJ_RS04800 0.22 -1.9 899,963 + MMJJ_RS04800 0.34 -1.5 900,073 - MMJJ_RS04800 0.51 -3.4 900,253 - MMJJ_RS04800 0.77 -2.7 900,254 + MMJJ_RS04800 0.78 -1.5 900,254 + MMJJ_RS04800 0.78 -2.7 900,261 - MMJJ_RS04800 0.79 -0.9 900,262 + MMJJ_RS04800 0.79 -1.5 900,267 - MMJJ_RS04800 0.80 -2.2 900,268 + MMJJ_RS04800 0.80 -3.1 900,268 + MMJJ_RS04800 0.80 -1.5 900,286 + +0.1 900,324 + MMJJ_RS04800 0.88 -1.2 900,324 + MMJJ_RS04800 0.88 -3.1 900,330 + MMJJ_RS04800 0.89 -3.5 900,330 + +0.1 900,429 + +1.3 900,483 - -1.5
Or see this region's nucleotide sequence