Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS04730 and MMJJ_RS04735 are separated by 144 nucleotides MMJJ_RS04735 and MMJJ_RS04740 are separated by 15 nucleotides MMJJ_RS04740 and MMJJ_RS04745 are separated by 77 nucleotides
MMJJ_RS04730: MMJJ_RS04730 - tyrosine decarboxylase MfnA, at 887,161 to 888,315
_RS04730
MMJJ_RS04735: MMJJ_RS04735 - fumarate hydratase, at 888,460 to 889,305
_RS04735
MMJJ_RS04740: MMJJ_RS04740 - histidinol phosphate phosphatase domain-containing protein, at 889,321 to 889,977
_RS04740
MMJJ_RS04745: MMJJ_RS04745 - cyclase family protein, at 890,055 to 890,624
_RS04745
Position (kb)
888
889
890 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 887.697 kb on - strand, within MMJJ_RS04730 at 887.716 kb on - strand, within MMJJ_RS04730 at 887.755 kb on + strand, within MMJJ_RS04730 at 887.830 kb on - strand, within MMJJ_RS04730 at 887.878 kb on + strand, within MMJJ_RS04730 at 888.006 kb on - strand, within MMJJ_RS04730 at 888.258 kb on + strand at 888.355 kb on - strand at 888.355 kb on - strand at 888.355 kb on - strand at 888.366 kb on + strand at 888.396 kb on - strand at 888.397 kb on + strand at 888.442 kb on + strand at 888.442 kb on + strand at 888.469 kb on - strand at 888.507 kb on - strand at 888.511 kb on - strand at 888.782 kb on + strand, within MMJJ_RS04735 at 889.051 kb on + strand, within MMJJ_RS04735 at 889.322 kb on - strand at 889.455 kb on - strand, within MMJJ_RS04740 at 889.796 kb on - strand, within MMJJ_RS04740 at 889.797 kb on + strand, within MMJJ_RS04740 at 890.014 kb on - strand at 890.015 kb on + strand at 890.015 kb on + strand at 890.016 kb on - strand at 890.016 kb on - strand at 890.017 kb on + strand at 890.020 kb on - strand at 890.020 kb on - strand at 890.020 kb on - strand at 890.020 kb on - strand at 890.020 kb on - strand at 890.020 kb on - strand at 890.021 kb on + strand at 890.023 kb on - strand at 890.026 kb on - strand at 890.026 kb on - strand at 890.031 kb on + strand at 890.034 kb on - strand at 890.034 kb on - strand at 890.042 kb on + strand at 890.071 kb on - strand at 890.072 kb on + strand at 890.104 kb on - strand at 890.104 kb on - strand at 890.155 kb on + strand, within MMJJ_RS04745 at 890.226 kb on + strand, within MMJJ_RS04745 at 890.268 kb on + strand, within MMJJ_RS04745
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE remove 887,697 - MMJJ_RS04730 0.46 -0.9 887,716 - MMJJ_RS04730 0.48 +0.1 887,755 + MMJJ_RS04730 0.51 -1.9 887,830 - MMJJ_RS04730 0.58 -1.5 887,878 + MMJJ_RS04730 0.62 -1.5 888,006 - MMJJ_RS04730 0.73 -0.9 888,258 + -2.2 888,355 - -2.6 888,355 - -0.9 888,355 - -4.9 888,366 + -4.6 888,396 - +0.6 888,397 + -3.8 888,442 + -4.2 888,442 + -1.2 888,469 - +1.5 888,507 - -1.9 888,511 - +0.1 888,782 + MMJJ_RS04735 0.38 -0.9 889,051 + MMJJ_RS04735 0.70 -1.5 889,322 - -0.9 889,455 - MMJJ_RS04740 0.20 -3.1 889,796 - MMJJ_RS04740 0.72 -0.6 889,797 + MMJJ_RS04740 0.72 -0.9 890,014 - -2.4 890,015 + -2.9 890,015 + -2.4 890,016 - +1.6 890,016 - -3.5 890,017 + -2.5 890,020 - -1.8 890,020 - +0.6 890,020 - +1.7 890,020 - +1.5 890,020 - -2.0 890,020 - -1.0 890,021 + -3.6 890,023 - -1.9 890,026 - +1.0 890,026 - -2.6 890,031 + +0.8 890,034 - +1.1 890,034 - -2.3 890,042 + +0.3 890,071 - +1.3 890,072 + +1.4 890,104 - +1.2 890,104 - -0.2 890,155 + MMJJ_RS04745 0.18 -2.1 890,226 + MMJJ_RS04745 0.30 +1.5 890,268 + MMJJ_RS04745 0.37 +1.4
Or see this region's nucleotide sequence