Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS03895 and MMJJ_RS03900 are separated by 211 nucleotides MMJJ_RS03900 and MMJJ_RS03905 are separated by 18 nucleotides MMJJ_RS03905 and MMJJ_RS03910 are separated by 25 nucleotides
MMJJ_RS03895: MMJJ_RS03895 - hydrogenase expression/formation protein HypE, at 727,607 to 728,611
_RS03895
MMJJ_RS03900: MMJJ_RS03900 - phosphosulfolactate synthase, at 728,823 to 729,578
_RS03900
MMJJ_RS03905: MMJJ_RS03905 - ATP-binding cassette domain-containing protein, at 729,597 to 730,388
_RS03905
MMJJ_RS03910: MMJJ_RS03910 - hypothetical protein, at 730,414 to 731,112
_RS03910
Position (kb) 728
729
730 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3 at 727.899 kb on + strand, within MMJJ_RS03895 at 727.925 kb on - strand, within MMJJ_RS03895 at 728.077 kb on - strand, within MMJJ_RS03895 at 728.231 kb on - strand, within MMJJ_RS03895 at 728.231 kb on - strand, within MMJJ_RS03895 at 728.427 kb on + strand, within MMJJ_RS03895 at 728.471 kb on + strand, within MMJJ_RS03895 at 728.535 kb on + strand at 728.629 kb on - strand at 728.640 kb on - strand at 728.640 kb on - strand at 728.640 kb on - strand at 728.644 kb on - strand at 728.644 kb on - strand at 728.661 kb on + strand at 728.681 kb on + strand at 728.691 kb on + strand at 728.691 kb on + strand at 728.703 kb on + strand at 728.732 kb on - strand at 728.732 kb on - strand at 728.732 kb on - strand at 728.735 kb on + strand at 728.736 kb on - strand at 728.743 kb on - strand at 728.750 kb on - strand at 728.750 kb on - strand at 728.750 kb on - strand at 728.750 kb on - strand at 728.750 kb on - strand at 728.751 kb on + strand at 728.751 kb on + strand at 728.752 kb on - strand at 728.753 kb on + strand at 728.755 kb on - strand at 728.756 kb on + strand at 728.756 kb on + strand at 728.760 kb on - strand at 728.795 kb on + strand at 728.868 kb on + strand at 728.875 kb on - strand at 728.876 kb on + strand at 728.918 kb on + strand, within MMJJ_RS03900 at 728.923 kb on - strand, within MMJJ_RS03900 at 728.935 kb on + strand, within MMJJ_RS03900 at 729.061 kb on - strand, within MMJJ_RS03900 at 729.061 kb on - strand, within MMJJ_RS03900 at 729.061 kb on - strand, within MMJJ_RS03900 at 729.061 kb on - strand, within MMJJ_RS03900 at 729.062 kb on + strand, within MMJJ_RS03900 at 729.062 kb on + strand, within MMJJ_RS03900 at 729.090 kb on - strand, within MMJJ_RS03900 at 729.091 kb on + strand, within MMJJ_RS03900 at 729.095 kb on + strand, within MMJJ_RS03900 at 729.095 kb on + strand, within MMJJ_RS03900 at 729.095 kb on + strand, within MMJJ_RS03900 at 729.104 kb on - strand, within MMJJ_RS03900 at 729.104 kb on - strand, within MMJJ_RS03900 at 729.104 kb on - strand, within MMJJ_RS03900 at 729.105 kb on + strand, within MMJJ_RS03900 at 729.113 kb on + strand, within MMJJ_RS03900 at 729.275 kb on + strand, within MMJJ_RS03900 at 729.397 kb on - strand, within MMJJ_RS03900 at 729.412 kb on - strand, within MMJJ_RS03900 at 729.427 kb on - strand, within MMJJ_RS03900 at 729.468 kb on + strand, within MMJJ_RS03900 at 729.468 kb on + strand, within MMJJ_RS03900 at 729.469 kb on - strand, within MMJJ_RS03900 at 729.472 kb on - strand, within MMJJ_RS03900 at 729.473 kb on + strand, within MMJJ_RS03900 at 729.503 kb on - strand at 729.504 kb on + strand at 729.504 kb on + strand at 729.580 kb on - strand at 729.620 kb on - strand at 729.667 kb on - strand at 729.667 kb on - strand at 729.667 kb on - strand at 729.668 kb on + strand at 729.668 kb on + strand at 729.668 kb on + strand at 729.668 kb on + strand at 729.673 kb on - strand at 729.864 kb on + strand, within MMJJ_RS03905 at 729.882 kb on + strand, within MMJJ_RS03905 at 729.965 kb on + strand, within MMJJ_RS03905 at 730.009 kb on - strand, within MMJJ_RS03905 at 730.016 kb on + strand, within MMJJ_RS03905 at 730.016 kb on + strand, within MMJJ_RS03905 at 730.087 kb on - strand, within MMJJ_RS03905 at 730.169 kb on + strand, within MMJJ_RS03905 at 730.172 kb on - strand, within MMJJ_RS03905 at 730.173 kb on + strand, within MMJJ_RS03905 at 730.173 kb on + strand, within MMJJ_RS03905 at 730.360 kb on - strand at 730.360 kb on - strand at 730.386 kb on - strand at 730.387 kb on + strand at 730.472 kb on - strand at 730.484 kb on - strand, within MMJJ_RS03910 at 730.484 kb on - strand, within MMJJ_RS03910 at 730.512 kb on - strand, within MMJJ_RS03910 at 730.527 kb on + strand, within MMJJ_RS03910 at 730.528 kb on - strand at 730.529 kb on + strand, within MMJJ_RS03910 at 730.529 kb on + strand, within MMJJ_RS03910 at 730.529 kb on + strand, within MMJJ_RS03910 at 730.530 kb on - strand, within MMJJ_RS03910 at 730.530 kb on - strand, within MMJJ_RS03910
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE remove 727,899 + MMJJ_RS03895 0.29 -3.4 727,925 - MMJJ_RS03895 0.32 -3.2 728,077 - MMJJ_RS03895 0.47 -2.7 728,231 - MMJJ_RS03895 0.62 -2.7 728,231 - MMJJ_RS03895 0.62 -1.5 728,427 + MMJJ_RS03895 0.82 -0.9 728,471 + MMJJ_RS03895 0.86 -3.4 728,535 + -3.5 728,629 - -2.9 728,640 - +0.1 728,640 - -1.8 728,640 - -0.6 728,644 - +2.0 728,644 - +0.6 728,661 + -0.9 728,681 + -1.8 728,691 + -1.9 728,691 + -1.0 728,703 + -1.2 728,732 - -1.5 728,732 - -2.0 728,732 - -0.9 728,735 + -1.5 728,736 - -2.8 728,743 - -3.0 728,750 - -2.9 728,750 - -3.8 728,750 - -0.3 728,750 - -3.7 728,750 - -1.7 728,751 + +0.0 728,751 + -3.4 728,752 - -5.3 728,753 + -2.8 728,755 - -1.0 728,756 + -1.5 728,756 + -4.1 728,760 - -3.4 728,795 + +3.3 728,868 + -1.8 728,875 - -1.9 728,876 + -1.8 728,918 + MMJJ_RS03900 0.13 -3.2 728,923 - MMJJ_RS03900 0.13 -4.4 728,935 + MMJJ_RS03900 0.15 -5.1 729,061 - MMJJ_RS03900 0.31 -2.7 729,061 - MMJJ_RS03900 0.31 -2.3 729,061 - MMJJ_RS03900 0.31 -0.3 729,061 - MMJJ_RS03900 0.31 -1.9 729,062 + MMJJ_RS03900 0.32 -3.9 729,062 + MMJJ_RS03900 0.32 -4.8 729,090 - MMJJ_RS03900 0.35 -1.0 729,091 + MMJJ_RS03900 0.35 -2.7 729,095 + MMJJ_RS03900 0.36 -4.0 729,095 + MMJJ_RS03900 0.36 -2.8 729,095 + MMJJ_RS03900 0.36 -2.8 729,104 - MMJJ_RS03900 0.37 -3.7 729,104 - MMJJ_RS03900 0.37 +0.1 729,104 - MMJJ_RS03900 0.37 -2.5 729,105 + MMJJ_RS03900 0.37 -3.2 729,113 + MMJJ_RS03900 0.38 -4.1 729,275 + MMJJ_RS03900 0.60 -3.4 729,397 - MMJJ_RS03900 0.76 -4.5 729,412 - MMJJ_RS03900 0.78 -3.8 729,427 - MMJJ_RS03900 0.80 -4.3 729,468 + MMJJ_RS03900 0.85 -4.5 729,468 + MMJJ_RS03900 0.85 -2.9 729,469 - MMJJ_RS03900 0.85 -2.2 729,472 - MMJJ_RS03900 0.86 -3.6 729,473 + MMJJ_RS03900 0.86 -1.4 729,503 - -4.1 729,504 + -1.7 729,504 + -2.3 729,580 - -3.5 729,620 - -2.4 729,667 - +2.0 729,667 - -1.7 729,667 - -2.3 729,668 + +1.6 729,668 + +1.9 729,668 + +0.1 729,668 + -1.6 729,673 - +1.9 729,864 + MMJJ_RS03905 0.34 -0.7 729,882 + MMJJ_RS03905 0.36 +1.6 729,965 + MMJJ_RS03905 0.46 +1.0 730,009 - MMJJ_RS03905 0.52 +1.6 730,016 + MMJJ_RS03905 0.53 +2.0 730,016 + MMJJ_RS03905 0.53 -1.1 730,087 - MMJJ_RS03905 0.62 -1.8 730,169 + MMJJ_RS03905 0.72 +1.5 730,172 - MMJJ_RS03905 0.73 +1.2 730,173 + MMJJ_RS03905 0.73 +1.9 730,173 + MMJJ_RS03905 0.73 -1.1 730,360 - +1.1 730,360 - -1.0 730,386 - +1.1 730,387 + +1.6 730,472 - +0.4 730,484 - MMJJ_RS03910 0.10 -1.8 730,484 - MMJJ_RS03910 0.10 +2.1 730,512 - MMJJ_RS03910 0.14 -0.7 730,527 + MMJJ_RS03910 0.16 +0.7 730,528 - -1.5 730,529 + MMJJ_RS03910 0.16 +0.9 730,529 + MMJJ_RS03910 0.16 +0.6 730,529 + MMJJ_RS03910 0.16 +1.1 730,530 - MMJJ_RS03910 0.17 -0.7 730,530 - MMJJ_RS03910 0.17 -1.9
Or see this region's nucleotide sequence