Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS02355 and MMJJ_RS02360 are separated by 616 nucleotides MMJJ_RS02360 and MMJJ_RS02365 are separated by 26 nucleotides MMJJ_RS02365 and MMJJ_RS02375 are separated by 180 nucleotides
MMJJ_RS02355: MMJJ_RS02355 - DUF2798 domain-containing protein, at 446,620 to 447,096
_RS02355
MMJJ_RS02360: MMJJ_RS02360 - hypothetical protein, at 447,713 to 448,192
_RS02360
MMJJ_RS02365: MMJJ_RS02365 - DUF2540 domain-containing protein, at 448,219 to 448,464
_RS02365
MMJJ_RS02375: MMJJ_RS02375 - site-specific integrase, at 448,645 to 449,469
_RS02375
Position (kb) 447
448
449 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 446.750 kb on - strand, within MMJJ_RS02355 at 446.751 kb on + strand, within MMJJ_RS02355 at 446.763 kb on - strand, within MMJJ_RS02355 at 446.764 kb on + strand, within MMJJ_RS02355 at 446.764 kb on + strand, within MMJJ_RS02355 at 446.764 kb on + strand, within MMJJ_RS02355 at 446.764 kb on + strand, within MMJJ_RS02355 at 446.771 kb on - strand, within MMJJ_RS02355 at 446.771 kb on - strand, within MMJJ_RS02355 at 446.772 kb on + strand, within MMJJ_RS02355 at 446.795 kb on + strand, within MMJJ_RS02355 at 446.815 kb on + strand, within MMJJ_RS02355 at 446.815 kb on + strand, within MMJJ_RS02355 at 446.823 kb on + strand, within MMJJ_RS02355 at 446.887 kb on + strand, within MMJJ_RS02355 at 446.887 kb on + strand, within MMJJ_RS02355 at 446.920 kb on - strand, within MMJJ_RS02355 at 446.920 kb on - strand, within MMJJ_RS02355 at 446.988 kb on - strand, within MMJJ_RS02355 at 447.075 kb on - strand at 447.126 kb on - strand at 447.178 kb on - strand at 447.187 kb on - strand at 447.187 kb on - strand at 447.189 kb on - strand at 447.190 kb on + strand at 447.227 kb on + strand at 447.301 kb on - strand at 447.315 kb on + strand at 447.327 kb on + strand at 447.329 kb on - strand at 447.350 kb on - strand at 447.350 kb on - strand at 447.350 kb on - strand at 447.351 kb on + strand at 447.351 kb on + strand at 447.351 kb on + strand at 447.354 kb on - strand at 447.354 kb on - strand at 447.354 kb on - strand at 447.354 kb on - strand at 447.354 kb on - strand at 447.354 kb on - strand at 447.354 kb on - strand at 447.354 kb on - strand at 447.354 kb on - strand at 447.354 kb on - strand at 447.354 kb on - strand at 447.354 kb on - strand at 447.354 kb on - strand at 447.354 kb on - strand at 447.355 kb on + strand at 447.355 kb on + strand at 447.355 kb on + strand at 447.355 kb on + strand at 447.355 kb on + strand at 447.355 kb on + strand at 447.355 kb on + strand at 447.355 kb on + strand at 447.355 kb on + strand at 447.355 kb on + strand at 447.355 kb on + strand at 447.382 kb on - strand at 447.386 kb on - strand at 447.386 kb on - strand at 447.386 kb on - strand at 447.387 kb on + strand at 447.393 kb on + strand at 447.406 kb on + strand at 447.406 kb on + strand at 447.408 kb on + strand at 447.408 kb on + strand at 447.446 kb on - strand at 447.446 kb on - strand at 447.452 kb on - strand at 447.460 kb on + strand at 447.562 kb on + strand at 447.576 kb on + strand at 447.626 kb on + strand at 447.634 kb on - strand at 447.634 kb on - strand at 447.649 kb on - strand at 447.649 kb on - strand at 447.650 kb on + strand at 447.675 kb on - strand at 447.675 kb on - strand at 447.678 kb on - strand at 447.678 kb on - strand at 447.679 kb on + strand at 447.679 kb on + strand at 447.679 kb on + strand at 447.685 kb on + strand at 447.697 kb on - strand at 447.697 kb on - strand at 447.822 kb on + strand, within MMJJ_RS02360 at 447.834 kb on + strand, within MMJJ_RS02360 at 447.834 kb on + strand, within MMJJ_RS02360 at 447.878 kb on + strand, within MMJJ_RS02360 at 447.883 kb on + strand, within MMJJ_RS02360 at 447.898 kb on - strand, within MMJJ_RS02360 at 447.898 kb on - strand, within MMJJ_RS02360 at 447.960 kb on + strand, within MMJJ_RS02360 at 447.979 kb on - strand, within MMJJ_RS02360 at 447.993 kb on - strand, within MMJJ_RS02360 at 448.065 kb on - strand, within MMJJ_RS02360 at 448.106 kb on + strand, within MMJJ_RS02360 at 448.163 kb on + strand at 448.171 kb on + strand at 448.190 kb on - strand at 448.191 kb on + strand at 448.209 kb on + strand at 448.245 kb on + strand, within MMJJ_RS02365 at 448.277 kb on + strand, within MMJJ_RS02365 at 448.277 kb on + strand, within MMJJ_RS02365 at 448.305 kb on - strand, within MMJJ_RS02365 at 448.306 kb on + strand, within MMJJ_RS02365 at 448.306 kb on + strand, within MMJJ_RS02365 at 448.433 kb on + strand, within MMJJ_RS02365 at 448.436 kb on - strand, within MMJJ_RS02365 at 448.437 kb on + strand, within MMJJ_RS02365 at 448.437 kb on + strand, within MMJJ_RS02365 at 448.713 kb on - strand at 448.713 kb on - strand at 448.725 kb on - strand at 448.725 kb on - strand at 448.761 kb on - strand, within MMJJ_RS02375 at 448.762 kb on + strand, within MMJJ_RS02375 at 448.789 kb on + strand, within MMJJ_RS02375 at 448.789 kb on + strand, within MMJJ_RS02375 at 448.791 kb on - strand, within MMJJ_RS02375 at 448.894 kb on - strand, within MMJJ_RS02375 at 448.894 kb on - strand, within MMJJ_RS02375 at 448.895 kb on + strand, within MMJJ_RS02375 at 448.905 kb on + strand, within MMJJ_RS02375 at 448.919 kb on - strand, within MMJJ_RS02375 at 448.920 kb on + strand, within MMJJ_RS02375 at 448.937 kb on - strand, within MMJJ_RS02375 at 448.938 kb on + strand, within MMJJ_RS02375 at 448.938 kb on + strand, within MMJJ_RS02375 at 448.938 kb on + strand, within MMJJ_RS02375 at 448.950 kb on - strand, within MMJJ_RS02375 at 448.951 kb on + strand, within MMJJ_RS02375 at 449.033 kb on - strand, within MMJJ_RS02375 at 449.034 kb on + strand, within MMJJ_RS02375 at 449.034 kb on + strand, within MMJJ_RS02375 at 449.075 kb on - strand, within MMJJ_RS02375 at 449.091 kb on - strand, within MMJJ_RS02375 at 449.111 kb on - strand, within MMJJ_RS02375 at 449.111 kb on - strand, within MMJJ_RS02375 at 449.130 kb on + strand, within MMJJ_RS02375 at 449.139 kb on + strand, within MMJJ_RS02375 at 449.143 kb on - strand, within MMJJ_RS02375 at 449.144 kb on + strand, within MMJJ_RS02375 at 449.144 kb on + strand, within MMJJ_RS02375 at 449.144 kb on + strand, within MMJJ_RS02375 at 449.144 kb on + strand, within MMJJ_RS02375 at 449.144 kb on + strand, within MMJJ_RS02375 at 449.146 kb on - strand, within MMJJ_RS02375 at 449.146 kb on - strand, within MMJJ_RS02375 at 449.155 kb on - strand, within MMJJ_RS02375 at 449.155 kb on - strand, within MMJJ_RS02375 at 449.155 kb on - strand, within MMJJ_RS02375 at 449.175 kb on - strand, within MMJJ_RS02375 at 449.176 kb on + strand, within MMJJ_RS02375 at 449.176 kb on + strand, within MMJJ_RS02375 at 449.176 kb on + strand, within MMJJ_RS02375
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE remove 446,750 - MMJJ_RS02355 0.27 +1.1 446,751 + MMJJ_RS02355 0.27 +1.3 446,763 - MMJJ_RS02355 0.30 +1.3 446,764 + MMJJ_RS02355 0.30 +1.3 446,764 + MMJJ_RS02355 0.30 +1.1 446,764 + MMJJ_RS02355 0.30 +0.7 446,764 + MMJJ_RS02355 0.30 -3.1 446,771 - MMJJ_RS02355 0.32 -0.1 446,771 - MMJJ_RS02355 0.32 -3.2 446,772 + MMJJ_RS02355 0.32 +0.8 446,795 + MMJJ_RS02355 0.37 +0.1 446,815 + MMJJ_RS02355 0.41 +1.5 446,815 + MMJJ_RS02355 0.41 +1.5 446,823 + MMJJ_RS02355 0.43 +1.1 446,887 + MMJJ_RS02355 0.56 +1.4 446,887 + MMJJ_RS02355 0.56 +0.7 446,920 - MMJJ_RS02355 0.63 +1.3 446,920 - MMJJ_RS02355 0.63 +1.6 446,988 - MMJJ_RS02355 0.77 +2.0 447,075 - -3.1 447,126 - -2.5 447,178 - +1.4 447,187 - +1.1 447,187 - -0.7 447,189 - -2.3 447,190 + +0.7 447,227 + -3.6 447,301 - -1.8 447,315 + -0.2 447,327 + -1.5 447,329 - +1.2 447,350 - +1.6 447,350 - +0.9 447,350 - -2.1 447,351 + +1.0 447,351 + +0.1 447,351 + +1.4 447,354 - +1.9 447,354 - +0.1 447,354 - -0.7 447,354 - -1.0 447,354 - +0.2 447,354 - +0.7 447,354 - -2.8 447,354 - -1.7 447,354 - -0.5 447,354 - +1.0 447,354 - +2.8 447,354 - -1.4 447,354 - -1.0 447,354 - +1.2 447,355 + -2.2 447,355 + -1.8 447,355 + +1.4 447,355 + -0.7 447,355 + +1.1 447,355 + -0.8 447,355 + +2.9 447,355 + +1.1 447,355 + -0.8 447,355 + -0.0 447,355 + +0.3 447,382 - +1.0 447,386 - -2.6 447,386 - -1.9 447,386 - +1.3 447,387 + +0.6 447,393 + +0.7 447,406 + +2.7 447,406 + -1.0 447,408 + +2.4 447,408 + -1.6 447,446 - +1.2 447,446 - +0.6 447,452 - -0.2 447,460 + +1.5 447,562 + -2.2 447,576 + -2.5 447,626 + -1.1 447,634 - -1.7 447,634 - +0.8 447,649 - -2.7 447,649 - -0.2 447,650 + +0.6 447,675 - +0.9 447,675 - -0.8 447,678 - -3.5 447,678 - -0.9 447,679 + +0.3 447,679 + +1.8 447,679 + -2.1 447,685 + +2.1 447,697 - +1.2 447,697 - +1.8 447,822 + MMJJ_RS02360 0.23 +0.4 447,834 + MMJJ_RS02360 0.25 -2.1 447,834 + MMJJ_RS02360 0.25 -3.6 447,878 + MMJJ_RS02360 0.34 +2.7 447,883 + MMJJ_RS02360 0.35 -0.5 447,898 - MMJJ_RS02360 0.39 -2.1 447,898 - MMJJ_RS02360 0.39 +1.5 447,960 + MMJJ_RS02360 0.51 -2.9 447,979 - MMJJ_RS02360 0.55 -2.8 447,993 - MMJJ_RS02360 0.58 -1.4 448,065 - MMJJ_RS02360 0.73 +1.0 448,106 + MMJJ_RS02360 0.82 +1.0 448,163 + -1.8 448,171 + +1.9 448,190 - +1.4 448,191 + +0.1 448,209 + +1.5 448,245 + MMJJ_RS02365 0.11 -1.7 448,277 + MMJJ_RS02365 0.24 -1.4 448,277 + MMJJ_RS02365 0.24 +0.8 448,305 - MMJJ_RS02365 0.35 +0.3 448,306 + MMJJ_RS02365 0.35 +1.6 448,306 + MMJJ_RS02365 0.35 -1.4 448,433 + MMJJ_RS02365 0.87 -2.1 448,436 - MMJJ_RS02365 0.88 -2.2 448,437 + MMJJ_RS02365 0.89 +1.6 448,437 + MMJJ_RS02365 0.89 -2.6 448,713 - -2.6 448,713 - -2.0 448,725 - -0.4 448,725 - +1.8 448,761 - MMJJ_RS02375 0.14 +0.7 448,762 + MMJJ_RS02375 0.14 +2.1 448,789 + MMJJ_RS02375 0.17 +2.0 448,789 + MMJJ_RS02375 0.17 -1.0 448,791 - MMJJ_RS02375 0.18 +1.7 448,894 - MMJJ_RS02375 0.30 +1.7 448,894 - MMJJ_RS02375 0.30 +3.0 448,895 + MMJJ_RS02375 0.30 -1.8 448,905 + MMJJ_RS02375 0.32 -2.2 448,919 - MMJJ_RS02375 0.33 +1.0 448,920 + MMJJ_RS02375 0.33 +0.4 448,937 - MMJJ_RS02375 0.35 +1.8 448,938 + MMJJ_RS02375 0.36 -0.7 448,938 + MMJJ_RS02375 0.36 -1.5 448,938 + MMJJ_RS02375 0.36 +2.5 448,950 - MMJJ_RS02375 0.37 -1.7 448,951 + MMJJ_RS02375 0.37 +1.3 449,033 - MMJJ_RS02375 0.47 -2.3 449,034 + MMJJ_RS02375 0.47 +1.2 449,034 + MMJJ_RS02375 0.47 -2.4 449,075 - MMJJ_RS02375 0.52 -0.0 449,091 - MMJJ_RS02375 0.54 +0.8 449,111 - MMJJ_RS02375 0.56 -1.5 449,111 - MMJJ_RS02375 0.56 +1.5 449,130 + MMJJ_RS02375 0.59 -2.6 449,139 + MMJJ_RS02375 0.60 +0.8 449,143 - MMJJ_RS02375 0.60 -3.1 449,144 + MMJJ_RS02375 0.60 +1.3 449,144 + MMJJ_RS02375 0.60 -1.9 449,144 + MMJJ_RS02375 0.60 -0.5 449,144 + MMJJ_RS02375 0.60 +1.3 449,144 + MMJJ_RS02375 0.60 -0.5 449,146 - MMJJ_RS02375 0.61 +3.6 449,146 - MMJJ_RS02375 0.61 -1.0 449,155 - MMJJ_RS02375 0.62 -0.7 449,155 - MMJJ_RS02375 0.62 +1.6 449,155 - MMJJ_RS02375 0.62 +0.8 449,175 - MMJJ_RS02375 0.64 +1.5 449,176 + MMJJ_RS02375 0.64 +0.5 449,176 + MMJJ_RS02375 0.64 -1.2 449,176 + MMJJ_RS02375 0.64 +1.5
Or see this region's nucleotide sequence