Strain Fitness in Methanococcus maripaludis JJ around MMJJ_RS01455

Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMMJJ_RS01450 and MMJJ_RS01455 are separated by 164 nucleotidesMMJJ_RS01455 and MMJJ_RS01460 are separated by 78 nucleotides MMJJ_RS01450: MMJJ_RS01450 - iron ABC transporter substrate-binding protein, at 283,642 to 284,769 _RS01450 MMJJ_RS01455: MMJJ_RS01455 - triose-phosphate isomerase, at 284,934 to 285,599 _RS01455 MMJJ_RS01460: MMJJ_RS01460 - 2-amino-3,7-dideoxy-D-threo-hept-6-ulosonate synthase, at 285,678 to 286,496 _RS01460 Position (kb) 284 285 286Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 283.936 kb on - strand, within MMJJ_RS01450at 283.937 kb on + strand, within MMJJ_RS01450at 283.960 kb on - strand, within MMJJ_RS01450at 284.221 kb on - strand, within MMJJ_RS01450at 284.222 kb on + strandat 284.222 kb on + strand, within MMJJ_RS01450at 284.222 kb on + strand, within MMJJ_RS01450at 284.236 kb on + strand, within MMJJ_RS01450at 284.279 kb on - strand, within MMJJ_RS01450at 284.282 kb on + strand, within MMJJ_RS01450at 284.321 kb on - strand, within MMJJ_RS01450at 284.387 kb on - strand, within MMJJ_RS01450at 284.387 kb on - strand, within MMJJ_RS01450at 284.388 kb on + strand, within MMJJ_RS01450at 284.388 kb on + strand, within MMJJ_RS01450at 284.388 kb on + strand, within MMJJ_RS01450at 284.391 kb on - strand, within MMJJ_RS01450at 284.401 kb on - strand, within MMJJ_RS01450at 284.401 kb on - strand, within MMJJ_RS01450at 284.402 kb on + strand, within MMJJ_RS01450at 284.413 kb on + strand, within MMJJ_RS01450at 284.464 kb on - strand, within MMJJ_RS01450at 284.465 kb on + strand, within MMJJ_RS01450at 284.467 kb on - strand, within MMJJ_RS01450at 284.467 kb on - strand, within MMJJ_RS01450at 284.505 kb on - strand, within MMJJ_RS01450at 284.506 kb on + strand, within MMJJ_RS01450at 284.506 kb on + strand, within MMJJ_RS01450at 284.508 kb on + strand, within MMJJ_RS01450at 284.556 kb on - strand, within MMJJ_RS01450at 284.556 kb on - strand, within MMJJ_RS01450at 284.556 kb on - strand, within MMJJ_RS01450at 284.557 kb on + strand, within MMJJ_RS01450at 284.569 kb on - strand, within MMJJ_RS01450at 284.569 kb on - strand, within MMJJ_RS01450at 284.570 kb on + strand, within MMJJ_RS01450at 284.570 kb on + strand, within MMJJ_RS01450at 284.570 kb on + strand, within MMJJ_RS01450at 284.573 kb on - strand, within MMJJ_RS01450at 284.608 kb on - strand, within MMJJ_RS01450at 284.608 kb on - strand, within MMJJ_RS01450at 284.609 kb on + strand, within MMJJ_RS01450at 284.609 kb on + strand, within MMJJ_RS01450at 284.609 kb on + strand, within MMJJ_RS01450at 284.609 kb on + strand, within MMJJ_RS01450at 284.609 kb on + strand, within MMJJ_RS01450at 284.609 kb on + strand, within MMJJ_RS01450at 284.609 kb on + strand, within MMJJ_RS01450at 284.617 kb on + strand, within MMJJ_RS01450at 284.655 kb on - strand, within MMJJ_RS01450at 284.664 kb on - strandat 284.677 kb on + strandat 284.697 kb on - strandat 284.698 kb on + strandat 284.709 kb on - strandat 284.769 kb on - strandat 284.770 kb on + strandat 284.796 kb on - strandat 284.824 kb on + strandat 284.837 kb on - strandat 284.842 kb on + strandat 284.878 kb on - strandat 284.929 kb on - strandat 285.481 kb on + strand, within MMJJ_RS01455at 285.574 kb on - strandat 285.586 kb on + strandat 285.599 kb on - strandat 285.619 kb on + strandat 285.620 kb on - strandat 285.624 kb on + strandat 285.644 kb on - strandat 285.649 kb on + strandat 285.649 kb on + strandat 285.649 kb on + strandat 285.696 kb on - strandat 286.105 kb on - strand, within MMJJ_RS01460at 286.543 kb on - strandat 286.546 kb on + strandat 286.547 kb on - strandat 286.547 kb on - strandat 286.548 kb on + strandat 286.548 kb on + strandat 286.548 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE
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283,936 - MMJJ_RS01450 0.26 +1.2
283,937 + MMJJ_RS01450 0.26 -2.4
283,960 - MMJJ_RS01450 0.28 -1.7
284,221 - MMJJ_RS01450 0.51 +1.1
284,222 + -1.0
284,222 + MMJJ_RS01450 0.51 -2.2
284,222 + MMJJ_RS01450 0.51 -2.7
284,236 + MMJJ_RS01450 0.53 +0.5
284,279 - MMJJ_RS01450 0.56 -3.6
284,282 + MMJJ_RS01450 0.57 +1.5
284,321 - MMJJ_RS01450 0.60 -2.1
284,387 - MMJJ_RS01450 0.66 -2.5
284,387 - MMJJ_RS01450 0.66 -2.7
284,388 + MMJJ_RS01450 0.66 -2.4
284,388 + MMJJ_RS01450 0.66 -0.4
284,388 + MMJJ_RS01450 0.66 +2.2
284,391 - MMJJ_RS01450 0.66 -2.2
284,401 - MMJJ_RS01450 0.67 -3.1
284,401 - MMJJ_RS01450 0.67 -0.0
284,402 + MMJJ_RS01450 0.67 +0.9
284,413 + MMJJ_RS01450 0.68 -0.3
284,464 - MMJJ_RS01450 0.73 +0.5
284,465 + MMJJ_RS01450 0.73 +0.6
284,467 - MMJJ_RS01450 0.73 +0.9
284,467 - MMJJ_RS01450 0.73 +0.6
284,505 - MMJJ_RS01450 0.77 -1.8
284,506 + MMJJ_RS01450 0.77 -2.5
284,506 + MMJJ_RS01450 0.77 +1.3
284,508 + MMJJ_RS01450 0.77 +0.7
284,556 - MMJJ_RS01450 0.81 +1.5
284,556 - MMJJ_RS01450 0.81 +1.0
284,556 - MMJJ_RS01450 0.81 +0.7
284,557 + MMJJ_RS01450 0.81 -1.6
284,569 - MMJJ_RS01450 0.82 +0.3
284,569 - MMJJ_RS01450 0.82 -2.0
284,570 + MMJJ_RS01450 0.82 -2.8
284,570 + MMJJ_RS01450 0.82 -2.4
284,570 + MMJJ_RS01450 0.82 +1.7
284,573 - MMJJ_RS01450 0.83 -1.4
284,608 - MMJJ_RS01450 0.86 +1.7
284,608 - MMJJ_RS01450 0.86 +0.1
284,609 + MMJJ_RS01450 0.86 +0.9
284,609 + MMJJ_RS01450 0.86 -3.3
284,609 + MMJJ_RS01450 0.86 -2.9
284,609 + MMJJ_RS01450 0.86 +1.4
284,609 + MMJJ_RS01450 0.86 -0.6
284,609 + MMJJ_RS01450 0.86 +1.6
284,609 + MMJJ_RS01450 0.86 +1.2
284,617 + MMJJ_RS01450 0.86 -2.2
284,655 - MMJJ_RS01450 0.90 -1.4
284,664 - -1.6
284,677 + +1.0
284,697 - -1.9
284,698 + -1.6
284,709 - +1.2
284,769 - -0.0
284,770 + +1.5
284,796 - +1.2
284,824 + -2.3
284,837 - +0.9
284,842 + -1.4
284,878 - -1.8
284,929 - -2.2
285,481 + MMJJ_RS01455 0.82 -3.2
285,574 - -2.0
285,586 + -2.0
285,599 - -1.6
285,619 + -0.0
285,620 - -2.0
285,624 + -2.6
285,644 - -2.2
285,649 + +0.8
285,649 + -1.7
285,649 + +0.4
285,696 - +1.7
286,105 - MMJJ_RS01460 0.52 -2.3
286,543 - -2.6
286,546 + -0.0
286,547 - +0.2
286,547 - -0.7
286,548 + -1.0
286,548 + -1.0
286,548 + -0.0

Or see this region's nucleotide sequence