Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS00515 and MMJJ_RS00520 overlap by 8 nucleotides MMJJ_RS00520 and MMJJ_RS00525 are separated by 120 nucleotides MMJJ_RS00525 and MMJJ_RS00530 are separated by 149 nucleotides
MMJJ_RS00515: MMJJ_RS00515 - cobalt transporter CbiM, at 97,247 to 97,885
_RS00515
MMJJ_RS00520: MMJJ_RS00520 - PDGLE domain-containing protein, at 97,878 to 98,204
_RS00520
MMJJ_RS00525: MMJJ_RS00525 - Dabb family protein, at 98,325 to 98,627
_RS00525
MMJJ_RS00530: MMJJ_RS00530 - cobalt ECF transporter T component CbiQ, at 98,777 to 99,544
_RS00530
Position (kb) 98
99 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3 at 97.329 kb on - strand, within MMJJ_RS00515 at 97.423 kb on - strand, within MMJJ_RS00515 at 97.423 kb on - strand, within MMJJ_RS00515 at 97.425 kb on - strand, within MMJJ_RS00515 at 97.425 kb on - strand, within MMJJ_RS00515 at 97.426 kb on + strand, within MMJJ_RS00515 at 97.438 kb on - strand, within MMJJ_RS00515 at 97.477 kb on + strand, within MMJJ_RS00515 at 97.477 kb on + strand, within MMJJ_RS00515 at 97.501 kb on + strand, within MMJJ_RS00515 at 97.501 kb on + strand, within MMJJ_RS00515 at 97.503 kb on - strand, within MMJJ_RS00515 at 97.503 kb on - strand, within MMJJ_RS00515 at 97.510 kb on + strand, within MMJJ_RS00515 at 97.583 kb on + strand, within MMJJ_RS00515 at 97.597 kb on + strand, within MMJJ_RS00515 at 97.597 kb on + strand, within MMJJ_RS00515 at 97.597 kb on + strand, within MMJJ_RS00515 at 97.608 kb on - strand, within MMJJ_RS00515 at 97.614 kb on + strand, within MMJJ_RS00515 at 97.616 kb on - strand, within MMJJ_RS00515 at 97.616 kb on - strand, within MMJJ_RS00515 at 97.616 kb on - strand, within MMJJ_RS00515 at 97.616 kb on - strand, within MMJJ_RS00515 at 97.616 kb on - strand, within MMJJ_RS00515 at 97.616 kb on - strand, within MMJJ_RS00515 at 97.616 kb on - strand, within MMJJ_RS00515 at 97.617 kb on + strand, within MMJJ_RS00515 at 97.617 kb on + strand, within MMJJ_RS00515 at 97.617 kb on + strand, within MMJJ_RS00515 at 97.617 kb on + strand, within MMJJ_RS00515 at 97.617 kb on + strand, within MMJJ_RS00515 at 97.617 kb on + strand, within MMJJ_RS00515 at 97.617 kb on + strand, within MMJJ_RS00515 at 97.687 kb on - strand, within MMJJ_RS00515 at 97.689 kb on - strand, within MMJJ_RS00515 at 97.689 kb on - strand, within MMJJ_RS00515 at 97.819 kb on + strand, within MMJJ_RS00515 at 97.851 kb on - strand at 97.852 kb on + strand at 97.860 kb on - strand at 97.884 kb on + strand at 97.901 kb on + strand at 97.901 kb on + strand at 97.901 kb on + strand at 97.901 kb on + strand at 97.913 kb on + strand, within MMJJ_RS00520 at 97.927 kb on - strand, within MMJJ_RS00520 at 97.928 kb on + strand, within MMJJ_RS00520 at 97.952 kb on + strand, within MMJJ_RS00520 at 98.001 kb on + strand, within MMJJ_RS00520 at 98.083 kb on + strand at 98.107 kb on - strand, within MMJJ_RS00520 at 98.125 kb on - strand, within MMJJ_RS00520 at 98.129 kb on - strand, within MMJJ_RS00520 at 98.130 kb on + strand, within MMJJ_RS00520 at 98.143 kb on - strand, within MMJJ_RS00520 at 98.144 kb on + strand, within MMJJ_RS00520 at 98.153 kb on + strand, within MMJJ_RS00520 at 98.153 kb on + strand, within MMJJ_RS00520 at 98.153 kb on + strand, within MMJJ_RS00520 at 98.177 kb on + strand at 98.250 kb on + strand at 98.266 kb on - strand at 98.270 kb on - strand at 98.271 kb on + strand at 98.271 kb on + strand at 98.280 kb on - strand at 98.281 kb on + strand at 98.285 kb on - strand at 98.286 kb on + strand at 98.305 kb on + strand at 98.320 kb on - strand at 98.320 kb on - strand at 98.321 kb on + strand at 98.321 kb on + strand at 98.324 kb on - strand at 98.464 kb on - strand, within MMJJ_RS00525 at 98.498 kb on + strand, within MMJJ_RS00525 at 98.534 kb on + strand, within MMJJ_RS00525 at 98.626 kb on + strand at 98.640 kb on + strand at 98.646 kb on - strand at 98.646 kb on - strand at 98.647 kb on + strand at 98.647 kb on + strand at 98.665 kb on - strand at 98.679 kb on - strand at 98.680 kb on + strand at 98.680 kb on + strand at 98.694 kb on - strand at 98.706 kb on - strand at 98.711 kb on - strand at 98.717 kb on + strand at 98.722 kb on - strand at 98.722 kb on - strand at 98.722 kb on - strand at 98.723 kb on + strand at 98.727 kb on - strand at 98.727 kb on - strand at 98.728 kb on + strand at 98.728 kb on + strand at 98.744 kb on - strand at 98.750 kb on + strand at 98.750 kb on + strand at 98.752 kb on - strand at 98.838 kb on - strand at 98.839 kb on + strand at 98.839 kb on + strand at 98.839 kb on + strand at 98.845 kb on - strand at 98.910 kb on - strand, within MMJJ_RS00530 at 98.910 kb on - strand, within MMJJ_RS00530 at 98.930 kb on - strand, within MMJJ_RS00530 at 99.054 kb on - strand, within MMJJ_RS00530 at 99.057 kb on - strand, within MMJJ_RS00530 at 99.058 kb on + strand, within MMJJ_RS00530 at 99.058 kb on + strand, within MMJJ_RS00530 at 99.083 kb on - strand, within MMJJ_RS00530 at 99.083 kb on - strand, within MMJJ_RS00530 at 99.084 kb on + strand, within MMJJ_RS00530 at 99.084 kb on + strand, within MMJJ_RS00530 at 99.084 kb on + strand, within MMJJ_RS00530 at 99.177 kb on + strand, within MMJJ_RS00530 at 99.177 kb on + strand, within MMJJ_RS00530 at 99.186 kb on - strand, within MMJJ_RS00530 at 99.187 kb on + strand, within MMJJ_RS00530 at 99.187 kb on + strand, within MMJJ_RS00530 at 99.187 kb on + strand, within MMJJ_RS00530 at 99.220 kb on - strand, within MMJJ_RS00530 at 99.221 kb on + strand, within MMJJ_RS00530 at 99.221 kb on + strand, within MMJJ_RS00530 at 99.226 kb on - strand, within MMJJ_RS00530 at 99.247 kb on - strand, within MMJJ_RS00530 at 99.248 kb on + strand, within MMJJ_RS00530 at 99.248 kb on + strand, within MMJJ_RS00530 at 99.248 kb on + strand, within MMJJ_RS00530 at 99.252 kb on + strand, within MMJJ_RS00530 at 99.255 kb on - strand, within MMJJ_RS00530 at 99.255 kb on - strand, within MMJJ_RS00530 at 99.255 kb on - strand, within MMJJ_RS00530 at 99.255 kb on - strand, within MMJJ_RS00530 at 99.255 kb on - strand, within MMJJ_RS00530 at 99.255 kb on - strand, within MMJJ_RS00530 at 99.255 kb on - strand, within MMJJ_RS00530 at 99.255 kb on - strand, within MMJJ_RS00530 at 99.256 kb on + strand, within MMJJ_RS00530 at 99.277 kb on + strand, within MMJJ_RS00530 at 99.313 kb on - strand, within MMJJ_RS00530 at 99.345 kb on - strand, within MMJJ_RS00530 at 99.350 kb on + strand, within MMJJ_RS00530 at 99.410 kb on - strand, within MMJJ_RS00530 at 99.411 kb on + strand, within MMJJ_RS00530 at 99.414 kb on - strand, within MMJJ_RS00530 at 99.482 kb on + strand at 99.505 kb on - strand at 99.505 kb on - strand at 99.514 kb on + strand at 99.516 kb on - strand at 99.577 kb on - strand at 99.577 kb on - strand at 99.578 kb on + strand at 99.578 kb on + strand at 99.584 kb on - strand at 99.584 kb on - strand at 99.587 kb on - strand at 99.587 kb on - strand at 99.587 kb on - strand at 99.587 kb on - strand at 99.588 kb on + strand at 99.588 kb on + strand at 99.588 kb on + strand at 99.590 kb on - strand at 99.590 kb on - strand at 99.590 kb on - strand at 99.590 kb on - strand at 99.590 kb on - strand at 99.590 kb on - strand at 99.590 kb on - strand at 99.591 kb on + strand at 99.591 kb on + strand at 99.591 kb on + strand at 99.591 kb on + strand at 99.591 kb on + strand at 99.592 kb on - strand at 99.592 kb on - strand at 99.592 kb on - strand at 99.592 kb on - strand at 99.593 kb on + strand at 99.596 kb on - strand at 99.597 kb on + strand at 99.603 kb on + strand at 99.603 kb on + strand at 99.603 kb on + strand at 99.605 kb on + strand at 99.605 kb on + strand at 99.606 kb on - strand at 99.606 kb on - strand at 99.606 kb on - strand at 99.606 kb on - strand at 99.606 kb on - strand at 99.607 kb on + strand at 99.607 kb on + strand at 99.607 kb on + strand at 99.607 kb on + strand at 99.607 kb on + strand at 99.607 kb on + strand at 99.617 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE remove 97,329 - MMJJ_RS00515 0.13 -0.1 97,423 - MMJJ_RS00515 0.28 -2.9 97,423 - MMJJ_RS00515 0.28 +1.1 97,425 - MMJJ_RS00515 0.28 +0.4 97,425 - MMJJ_RS00515 0.28 -0.4 97,426 + MMJJ_RS00515 0.28 -3.4 97,438 - MMJJ_RS00515 0.30 -3.0 97,477 + MMJJ_RS00515 0.36 -2.2 97,477 + MMJJ_RS00515 0.36 -4.1 97,501 + MMJJ_RS00515 0.40 -3.4 97,501 + MMJJ_RS00515 0.40 +1.1 97,503 - MMJJ_RS00515 0.40 -0.6 97,503 - MMJJ_RS00515 0.40 +0.8 97,510 + MMJJ_RS00515 0.41 -2.3 97,583 + MMJJ_RS00515 0.53 +0.4 97,597 + MMJJ_RS00515 0.55 -1.1 97,597 + MMJJ_RS00515 0.55 +0.5 97,597 + MMJJ_RS00515 0.55 +0.5 97,608 - MMJJ_RS00515 0.56 -1.7 97,614 + MMJJ_RS00515 0.57 +1.1 97,616 - MMJJ_RS00515 0.58 -3.7 97,616 - MMJJ_RS00515 0.58 +1.7 97,616 - MMJJ_RS00515 0.58 +1.0 97,616 - MMJJ_RS00515 0.58 +0.7 97,616 - MMJJ_RS00515 0.58 +1.2 97,616 - MMJJ_RS00515 0.58 -0.4 97,616 - MMJJ_RS00515 0.58 -3.1 97,617 + MMJJ_RS00515 0.58 -0.0 97,617 + MMJJ_RS00515 0.58 +0.5 97,617 + MMJJ_RS00515 0.58 -2.0 97,617 + MMJJ_RS00515 0.58 +0.4 97,617 + MMJJ_RS00515 0.58 +0.2 97,617 + MMJJ_RS00515 0.58 -2.6 97,617 + MMJJ_RS00515 0.58 -1.8 97,687 - MMJJ_RS00515 0.69 -1.0 97,689 - MMJJ_RS00515 0.69 -0.0 97,689 - MMJJ_RS00515 0.69 +1.0 97,819 + MMJJ_RS00515 0.90 -2.4 97,851 - -0.4 97,852 + +1.1 97,860 - +0.1 97,884 + +0.7 97,901 + +0.3 97,901 + -2.1 97,901 + +0.7 97,901 + +0.0 97,913 + MMJJ_RS00520 0.11 +0.5 97,927 - MMJJ_RS00520 0.15 -2.9 97,928 + MMJJ_RS00520 0.15 -1.8 97,952 + MMJJ_RS00520 0.23 -2.5 98,001 + MMJJ_RS00520 0.38 -1.2 98,083 + -1.6 98,107 - MMJJ_RS00520 0.70 -0.8 98,125 - MMJJ_RS00520 0.76 +1.6 98,129 - MMJJ_RS00520 0.77 +1.0 98,130 + MMJJ_RS00520 0.77 -4.6 98,143 - MMJJ_RS00520 0.81 -2.2 98,144 + MMJJ_RS00520 0.81 -0.2 98,153 + MMJJ_RS00520 0.84 +1.2 98,153 + MMJJ_RS00520 0.84 +0.8 98,153 + MMJJ_RS00520 0.84 +1.1 98,177 + -2.2 98,250 + +2.0 98,266 - +1.8 98,270 - -0.7 98,271 + +1.2 98,271 + -2.1 98,280 - -1.5 98,281 + -0.7 98,285 - +0.7 98,286 + -1.9 98,305 + +2.3 98,320 - -0.8 98,320 - +1.4 98,321 + -1.4 98,321 + +1.2 98,324 - -1.6 98,464 - MMJJ_RS00525 0.46 -2.1 98,498 + MMJJ_RS00525 0.57 -2.0 98,534 + MMJJ_RS00525 0.69 -2.5 98,626 + +0.6 98,640 + +0.7 98,646 - -1.4 98,646 - -1.9 98,647 + +1.5 98,647 + +1.0 98,665 - +1.2 98,679 - -3.2 98,680 + -1.0 98,680 + +0.6 98,694 - +0.0 98,706 - -0.0 98,711 - -1.6 98,717 + +0.5 98,722 - -0.5 98,722 - -2.9 98,722 - +0.9 98,723 + -3.0 98,727 - -0.0 98,727 - +1.1 98,728 + +0.6 98,728 + +1.3 98,744 - -0.2 98,750 + +1.5 98,750 + +0.6 98,752 - +0.7 98,838 - +0.9 98,839 + +0.6 98,839 + +1.4 98,839 + +1.5 98,845 - +1.3 98,910 - MMJJ_RS00530 0.17 -2.9 98,910 - MMJJ_RS00530 0.17 -2.8 98,930 - MMJJ_RS00530 0.20 +1.2 99,054 - MMJJ_RS00530 0.36 +0.1 99,057 - MMJJ_RS00530 0.36 +1.4 99,058 + MMJJ_RS00530 0.37 -1.0 99,058 + MMJJ_RS00530 0.37 +0.5 99,083 - MMJJ_RS00530 0.40 -1.4 99,083 - MMJJ_RS00530 0.40 -1.1 99,084 + MMJJ_RS00530 0.40 +0.7 99,084 + MMJJ_RS00530 0.40 +1.0 99,084 + MMJJ_RS00530 0.40 -2.6 99,177 + MMJJ_RS00530 0.52 +1.3 99,177 + MMJJ_RS00530 0.52 -0.3 99,186 - MMJJ_RS00530 0.53 -1.4 99,187 + MMJJ_RS00530 0.53 +1.6 99,187 + MMJJ_RS00530 0.53 +0.4 99,187 + MMJJ_RS00530 0.53 +0.4 99,220 - MMJJ_RS00530 0.58 -1.8 99,221 + MMJJ_RS00530 0.58 -0.2 99,221 + MMJJ_RS00530 0.58 -1.9 99,226 - MMJJ_RS00530 0.58 +0.4 99,247 - MMJJ_RS00530 0.61 +0.5 99,248 + MMJJ_RS00530 0.61 -2.4 99,248 + MMJJ_RS00530 0.61 -2.4 99,248 + MMJJ_RS00530 0.61 -0.3 99,252 + MMJJ_RS00530 0.62 +1.9 99,255 - MMJJ_RS00530 0.62 -2.0 99,255 - MMJJ_RS00530 0.62 -0.1 99,255 - MMJJ_RS00530 0.62 -1.1 99,255 - MMJJ_RS00530 0.62 -2.2 99,255 - MMJJ_RS00530 0.62 -0.6 99,255 - MMJJ_RS00530 0.62 +1.7 99,255 - MMJJ_RS00530 0.62 -3.4 99,255 - MMJJ_RS00530 0.62 +1.2 99,256 + MMJJ_RS00530 0.62 -2.8 99,277 + MMJJ_RS00530 0.65 -0.0 99,313 - MMJJ_RS00530 0.70 -1.2 99,345 - MMJJ_RS00530 0.74 +0.9 99,350 + MMJJ_RS00530 0.75 +0.8 99,410 - MMJJ_RS00530 0.82 +0.3 99,411 + MMJJ_RS00530 0.83 +0.1 99,414 - MMJJ_RS00530 0.83 +1.8 99,482 + +0.5 99,505 - +1.0 99,505 - -3.3 99,514 + +1.1 99,516 - -4.7 99,577 - -2.6 99,577 - +3.0 99,578 + +1.1 99,578 + +1.1 99,584 - +0.9 99,584 - +1.2 99,587 - +0.7 99,587 - +1.0 99,587 - +0.5 99,587 - +0.3 99,588 + -1.0 99,588 + -3.6 99,588 + +1.1 99,590 - -0.3 99,590 - +1.6 99,590 - +1.4 99,590 - -5.5 99,590 - -3.2 99,590 - +0.7 99,590 - -1.0 99,591 + +1.6 99,591 + +0.6 99,591 + -1.2 99,591 + +0.2 99,591 + +0.9 99,592 - -1.2 99,592 - +1.6 99,592 - +1.4 99,592 - +0.7 99,593 + +0.7 99,596 - -0.8 99,597 + +1.6 99,603 + +0.7 99,603 + +1.7 99,603 + -2.7 99,605 + +0.7 99,605 + +1.2 99,606 - -0.9 99,606 - +1.4 99,606 - -1.5 99,606 - +1.5 99,606 - +1.2 99,607 + +0.6 99,607 + -1.8 99,607 + +1.0 99,607 + -2.1 99,607 + -2.5 99,607 + +1.7 99,617 + -3.5
Or see this region's nucleotide sequence