Experiment: L-Glutamine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS15520 and RR42_RS15525 are separated by 186 nucleotides RR42_RS15525 and RR42_RS15530 overlap by 4 nucleotides RR42_RS15530 and RR42_RS15535 are separated by 74 nucleotides RR42_RS15535 and RR42_RS15540 are separated by 95 nucleotides
RR42_RS15520: RR42_RS15520 - hypothetical protein, at 3,354,208 to 3,354,639
_RS15520
RR42_RS15525: RR42_RS15525 - hypothetical protein, at 3,354,826 to 3,355,266
_RS15525
RR42_RS15530: RR42_RS15530 - membrane protein, at 3,355,263 to 3,356,195
_RS15530
RR42_RS15535: RR42_RS15535 - hypothetical protein, at 3,356,270 to 3,356,818
_RS15535
RR42_RS15540: RR42_RS15540 - hypothetical protein, at 3,356,914 to 3,358,347
_RS15540
Position (kb)
3355
3356
3357 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3354.325 kb on + strand, within RR42_RS15520 at 3354.325 kb on + strand, within RR42_RS15520 at 3354.326 kb on - strand, within RR42_RS15520 at 3354.326 kb on - strand, within RR42_RS15520 at 3354.397 kb on + strand, within RR42_RS15520 at 3354.415 kb on + strand, within RR42_RS15520 at 3354.415 kb on + strand, within RR42_RS15520 at 3354.481 kb on + strand, within RR42_RS15520 at 3354.482 kb on - strand, within RR42_RS15520 at 3354.526 kb on + strand, within RR42_RS15520 at 3354.526 kb on + strand, within RR42_RS15520 at 3354.527 kb on - strand, within RR42_RS15520 at 3354.527 kb on - strand, within RR42_RS15520 at 3354.527 kb on - strand, within RR42_RS15520 at 3354.715 kb on + strand at 3354.716 kb on - strand at 3354.756 kb on - strand at 3354.790 kb on + strand at 3354.998 kb on - strand, within RR42_RS15525 at 3355.378 kb on - strand, within RR42_RS15530 at 3355.458 kb on - strand, within RR42_RS15530 at 3355.556 kb on + strand, within RR42_RS15530 at 3355.557 kb on - strand, within RR42_RS15530 at 3355.726 kb on - strand, within RR42_RS15530 at 3355.746 kb on + strand, within RR42_RS15530 at 3355.746 kb on + strand, within RR42_RS15530 at 3355.746 kb on + strand, within RR42_RS15530 at 3355.746 kb on + strand, within RR42_RS15530 at 3355.746 kb on + strand, within RR42_RS15530 at 3355.746 kb on + strand, within RR42_RS15530 at 3355.746 kb on + strand, within RR42_RS15530 at 3355.746 kb on + strand, within RR42_RS15530 at 3355.746 kb on + strand, within RR42_RS15530 at 3355.746 kb on + strand, within RR42_RS15530 at 3355.747 kb on - strand, within RR42_RS15530 at 3355.747 kb on - strand, within RR42_RS15530 at 3355.747 kb on - strand, within RR42_RS15530 at 3355.747 kb on - strand, within RR42_RS15530 at 3355.747 kb on - strand, within RR42_RS15530 at 3355.748 kb on + strand, within RR42_RS15530 at 3355.749 kb on - strand, within RR42_RS15530 at 3355.791 kb on + strand, within RR42_RS15530 at 3355.792 kb on - strand, within RR42_RS15530 at 3355.970 kb on - strand, within RR42_RS15530 at 3355.970 kb on - strand, within RR42_RS15530 at 3356.042 kb on + strand, within RR42_RS15530 at 3356.043 kb on - strand, within RR42_RS15530 at 3356.043 kb on - strand, within RR42_RS15530 at 3356.165 kb on + strand at 3356.188 kb on + strand at 3356.188 kb on + strand at 3356.188 kb on + strand at 3356.188 kb on + strand at 3356.188 kb on + strand at 3356.188 kb on + strand at 3356.188 kb on + strand at 3356.188 kb on + strand at 3356.189 kb on - strand at 3356.189 kb on - strand at 3356.189 kb on - strand at 3356.342 kb on + strand, within RR42_RS15535 at 3356.342 kb on + strand, within RR42_RS15535 at 3356.443 kb on - strand, within RR42_RS15535 at 3356.446 kb on + strand, within RR42_RS15535 at 3356.661 kb on - strand, within RR42_RS15535 at 3356.715 kb on - strand, within RR42_RS15535 at 3356.715 kb on - strand, within RR42_RS15535 at 3356.796 kb on + strand at 3356.796 kb on + strand at 3356.797 kb on - strand at 3356.797 kb on - strand at 3357.057 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (C) remove 3,354,325 + RR42_RS15520 0.27 +0.1 3,354,325 + RR42_RS15520 0.27 +0.3 3,354,326 - RR42_RS15520 0.27 -2.6 3,354,326 - RR42_RS15520 0.27 +0.5 3,354,397 + RR42_RS15520 0.44 -0.9 3,354,415 + RR42_RS15520 0.48 +0.7 3,354,415 + RR42_RS15520 0.48 -0.9 3,354,481 + RR42_RS15520 0.63 -0.5 3,354,482 - RR42_RS15520 0.63 +0.5 3,354,526 + RR42_RS15520 0.74 +0.1 3,354,526 + RR42_RS15520 0.74 +0.1 3,354,527 - RR42_RS15520 0.74 +0.2 3,354,527 - RR42_RS15520 0.74 +0.3 3,354,527 - RR42_RS15520 0.74 -0.3 3,354,715 + -0.2 3,354,716 - -0.8 3,354,756 - +0.5 3,354,790 + -0.3 3,354,998 - RR42_RS15525 0.39 -1.3 3,355,378 - RR42_RS15530 0.12 +0.7 3,355,458 - RR42_RS15530 0.21 -0.0 3,355,556 + RR42_RS15530 0.31 -0.5 3,355,557 - RR42_RS15530 0.32 -1.7 3,355,726 - RR42_RS15530 0.50 +0.2 3,355,746 + RR42_RS15530 0.52 -0.6 3,355,746 + RR42_RS15530 0.52 +0.5 3,355,746 + RR42_RS15530 0.52 -0.4 3,355,746 + RR42_RS15530 0.52 +0.1 3,355,746 + RR42_RS15530 0.52 -0.6 3,355,746 + RR42_RS15530 0.52 -0.9 3,355,746 + RR42_RS15530 0.52 +1.4 3,355,746 + RR42_RS15530 0.52 -0.2 3,355,746 + RR42_RS15530 0.52 -0.2 3,355,746 + RR42_RS15530 0.52 -0.2 3,355,747 - RR42_RS15530 0.52 -0.3 3,355,747 - RR42_RS15530 0.52 -0.3 3,355,747 - RR42_RS15530 0.52 -0.1 3,355,747 - RR42_RS15530 0.52 +0.1 3,355,747 - RR42_RS15530 0.52 +0.2 3,355,748 + RR42_RS15530 0.52 -0.1 3,355,749 - RR42_RS15530 0.52 -1.7 3,355,791 + RR42_RS15530 0.57 -0.7 3,355,792 - RR42_RS15530 0.57 +0.3 3,355,970 - RR42_RS15530 0.76 -1.1 3,355,970 - RR42_RS15530 0.76 +0.7 3,356,042 + RR42_RS15530 0.83 +0.5 3,356,043 - RR42_RS15530 0.84 +1.3 3,356,043 - RR42_RS15530 0.84 +0.3 3,356,165 + -1.0 3,356,188 + -0.2 3,356,188 + +1.8 3,356,188 + -0.1 3,356,188 + +0.1 3,356,188 + +1.1 3,356,188 + -0.4 3,356,188 + +0.4 3,356,188 + -1.2 3,356,189 - -0.5 3,356,189 - -0.6 3,356,189 - +1.5 3,356,342 + RR42_RS15535 0.13 -1.7 3,356,342 + RR42_RS15535 0.13 -0.8 3,356,443 - RR42_RS15535 0.32 +0.5 3,356,446 + RR42_RS15535 0.32 -0.1 3,356,661 - RR42_RS15535 0.71 -0.2 3,356,715 - RR42_RS15535 0.81 -0.9 3,356,715 - RR42_RS15535 0.81 +0.1 3,356,796 + +0.2 3,356,796 + +0.1 3,356,797 - +0.3 3,356,797 - +0.3 3,357,057 - +0.1
Or see this region's nucleotide sequence