Experiment: L-Glutamine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS01210 and RR42_RS01215 are separated by 78 nucleotides RR42_RS01215 and RR42_RS01220 are separated by 30 nucleotides RR42_RS01220 and RR42_RS01225 are separated by 30 nucleotides RR42_RS01225 and RR42_RS01230 are separated by 184 nucleotides
RR42_RS01210: RR42_RS01210 - n-acetyl-gamma-glutamyl-phosphate reductase, at 275,725 to 276,672
_RS01210
RR42_RS01215: RR42_RS01215 - hypothetical protein, at 276,751 to 276,939
_RS01215
RR42_RS01220: RR42_RS01220 - exodeoxyribonuclease III, at 276,970 to 277,746
_RS01220
RR42_RS01225: RR42_RS01225 - orotate phosphoribosyltransferase, at 277,777 to 278,466
_RS01225
RR42_RS01230: RR42_RS01230 - acetoacetate decarboxylase, at 278,651 to 279,391
_RS01230
Position (kb) 276
277
278 Strain fitness (log2 ratio) -2
-1
0
1
2 at 276.230 kb on + strand, within RR42_RS01210 at 276.399 kb on - strand, within RR42_RS01210 at 276.450 kb on + strand, within RR42_RS01210 at 276.451 kb on - strand, within RR42_RS01210 at 276.612 kb on - strand at 276.652 kb on + strand at 276.653 kb on - strand at 276.653 kb on - strand at 276.653 kb on - strand at 276.653 kb on - strand at 276.692 kb on - strand at 276.692 kb on - strand at 276.733 kb on + strand at 276.733 kb on + strand at 276.733 kb on + strand at 276.733 kb on + strand at 276.734 kb on - strand at 276.737 kb on + strand at 276.737 kb on + strand at 276.737 kb on + strand at 276.737 kb on + strand at 276.737 kb on + strand at 276.737 kb on + strand at 276.737 kb on + strand at 276.738 kb on - strand at 276.738 kb on - strand at 276.738 kb on - strand at 276.781 kb on - strand, within RR42_RS01215 at 276.784 kb on + strand, within RR42_RS01215 at 276.784 kb on + strand, within RR42_RS01215 at 276.785 kb on - strand, within RR42_RS01215 at 276.785 kb on - strand, within RR42_RS01215 at 276.785 kb on - strand, within RR42_RS01215 at 276.938 kb on - strand at 276.965 kb on - strand at 276.965 kb on - strand at 276.965 kb on - strand at 276.971 kb on + strand at 276.971 kb on + strand at 276.972 kb on - strand at 276.972 kb on - strand at 276.972 kb on - strand at 277.031 kb on + strand at 277.142 kb on + strand, within RR42_RS01220 at 277.142 kb on + strand, within RR42_RS01220 at 277.142 kb on + strand, within RR42_RS01220 at 277.142 kb on + strand, within RR42_RS01220 at 277.142 kb on + strand, within RR42_RS01220 at 277.143 kb on - strand, within RR42_RS01220 at 277.143 kb on - strand, within RR42_RS01220 at 277.143 kb on - strand, within RR42_RS01220 at 277.143 kb on - strand, within RR42_RS01220 at 277.143 kb on - strand, within RR42_RS01220 at 277.421 kb on + strand, within RR42_RS01220 at 277.421 kb on + strand, within RR42_RS01220 at 277.421 kb on + strand, within RR42_RS01220 at 277.422 kb on - strand, within RR42_RS01220 at 277.448 kb on + strand, within RR42_RS01220 at 277.463 kb on + strand, within RR42_RS01220 at 277.548 kb on + strand, within RR42_RS01220 at 277.548 kb on + strand, within RR42_RS01220 at 277.549 kb on - strand, within RR42_RS01220 at 277.568 kb on + strand, within RR42_RS01220 at 277.577 kb on + strand, within RR42_RS01220 at 277.577 kb on + strand, within RR42_RS01220 at 277.734 kb on + strand at 278.696 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (C) remove 276,230 + RR42_RS01210 0.53 -0.9 276,399 - RR42_RS01210 0.71 -0.1 276,450 + RR42_RS01210 0.76 +2.0 276,451 - RR42_RS01210 0.77 +0.1 276,612 - -0.6 276,652 + -0.4 276,653 - -0.1 276,653 - -0.4 276,653 - +0.4 276,653 - -0.6 276,692 - -0.8 276,692 - -1.0 276,733 + +0.2 276,733 + -0.2 276,733 + +2.2 276,733 + -1.1 276,734 - -1.8 276,737 + +1.7 276,737 + -0.2 276,737 + -0.1 276,737 + -0.0 276,737 + +0.5 276,737 + +0.7 276,737 + -1.4 276,738 - -0.4 276,738 - +0.2 276,738 - +0.5 276,781 - RR42_RS01215 0.16 +0.0 276,784 + RR42_RS01215 0.17 -0.4 276,784 + RR42_RS01215 0.17 -1.0 276,785 - RR42_RS01215 0.18 +0.1 276,785 - RR42_RS01215 0.18 -0.6 276,785 - RR42_RS01215 0.18 -0.9 276,938 - +0.9 276,965 - -1.1 276,965 - +0.8 276,965 - +1.2 276,971 + +0.7 276,971 + +0.4 276,972 - +0.5 276,972 - +1.9 276,972 - -0.2 277,031 + +0.7 277,142 + RR42_RS01220 0.22 -0.5 277,142 + RR42_RS01220 0.22 +1.9 277,142 + RR42_RS01220 0.22 -0.4 277,142 + RR42_RS01220 0.22 +0.2 277,142 + RR42_RS01220 0.22 +0.7 277,143 - RR42_RS01220 0.22 -0.1 277,143 - RR42_RS01220 0.22 -0.4 277,143 - RR42_RS01220 0.22 +1.8 277,143 - RR42_RS01220 0.22 +1.2 277,143 - RR42_RS01220 0.22 +0.2 277,421 + RR42_RS01220 0.58 +0.7 277,421 + RR42_RS01220 0.58 +0.8 277,421 + RR42_RS01220 0.58 +0.0 277,422 - RR42_RS01220 0.58 -0.3 277,448 + RR42_RS01220 0.62 -0.8 277,463 + RR42_RS01220 0.63 +0.1 277,548 + RR42_RS01220 0.74 +0.7 277,548 + RR42_RS01220 0.74 -0.4 277,549 - RR42_RS01220 0.75 +1.0 277,568 + RR42_RS01220 0.77 -0.4 277,577 + RR42_RS01220 0.78 +0.1 277,577 + RR42_RS01220 0.78 -1.1 277,734 + +0.7 278,696 - -0.5
Or see this region's nucleotide sequence