Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_5850

Experiment: sodium fluoride 12.5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_5848 and Pf1N1B4_5849 overlap by 1 nucleotidesPf1N1B4_5849 and Pf1N1B4_5850 overlap by 50 nucleotidesPf1N1B4_5850 and Pf1N1B4_5851 are separated by 247 nucleotides Pf1N1B4_5848 - Precorrin-6A synthase (EC 2.1.1.152), at 282,097 to 282,852 _5848 Pf1N1B4_5849 - Probable phosphoglycerate mutase, at 282,852 to 283,337 _5849 Pf1N1B4_5850 - hypothetical protein, at 283,288 to 283,509 _5850 Pf1N1B4_5851 - Transcriptional regulator, RpiR family, at 283,757 to 284,611 _5851 Position (kb) 283 284Strain fitness (log2 ratio) -2 -1 0 1 2at 282.393 kb on + strand, within Pf1N1B4_5848at 282.393 kb on + strand, within Pf1N1B4_5848at 282.393 kb on + strand, within Pf1N1B4_5848at 282.393 kb on + strandat 282.393 kb on + strandat 282.393 kb on + strand, within Pf1N1B4_5848at 282.393 kb on + strand, within Pf1N1B4_5848at 282.394 kb on - strand, within Pf1N1B4_5848at 282.394 kb on - strand, within Pf1N1B4_5848at 282.394 kb on - strandat 282.394 kb on - strand, within Pf1N1B4_5848at 282.394 kb on - strand, within Pf1N1B4_5848at 282.394 kb on - strandat 282.394 kb on - strand, within Pf1N1B4_5848at 282.495 kb on + strand, within Pf1N1B4_5848at 282.496 kb on - strandat 282.503 kb on + strand, within Pf1N1B4_5848at 282.503 kb on + strand, within Pf1N1B4_5848at 282.503 kb on + strand, within Pf1N1B4_5848at 282.503 kb on + strand, within Pf1N1B4_5848at 282.503 kb on + strand, within Pf1N1B4_5848at 282.504 kb on - strand, within Pf1N1B4_5848at 282.504 kb on - strand, within Pf1N1B4_5848at 282.623 kb on + strandat 282.692 kb on + strand, within Pf1N1B4_5848at 282.692 kb on + strand, within Pf1N1B4_5848at 282.693 kb on - strand, within Pf1N1B4_5848at 282.888 kb on + strandat 282.889 kb on - strandat 283.020 kb on - strandat 283.020 kb on - strand, within Pf1N1B4_5849at 283.530 kb on + strandat 283.530 kb on + strandat 283.531 kb on - strandat 283.531 kb on - strandat 283.683 kb on + strandat 283.683 kb on + strandat 283.801 kb on + strandat 283.824 kb on + strandat 283.825 kb on - strandat 284.036 kb on - strand, within Pf1N1B4_5851at 284.058 kb on - strand, within Pf1N1B4_5851at 284.058 kb on - strand, within Pf1N1B4_5851at 284.118 kb on + strand, within Pf1N1B4_5851at 284.119 kb on - strand, within Pf1N1B4_5851at 284.119 kb on - strand, within Pf1N1B4_5851at 284.402 kb on + strand, within Pf1N1B4_5851at 284.403 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction sodium fluoride 12.5 mM
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282,393 + Pf1N1B4_5848 0.39 -0.1
282,393 + Pf1N1B4_5848 0.39 +0.9
282,393 + Pf1N1B4_5848 0.39 +0.5
282,393 + +0.4
282,393 + +2.0
282,393 + Pf1N1B4_5848 0.39 -0.3
282,393 + Pf1N1B4_5848 0.39 -0.2
282,394 - Pf1N1B4_5848 0.39 -0.0
282,394 - Pf1N1B4_5848 0.39 -1.3
282,394 - -0.6
282,394 - Pf1N1B4_5848 0.39 -0.6
282,394 - Pf1N1B4_5848 0.39 +0.2
282,394 - -1.0
282,394 - Pf1N1B4_5848 0.39 -1.3
282,495 + Pf1N1B4_5848 0.53 -1.3
282,496 - -0.3
282,503 + Pf1N1B4_5848 0.54 -0.3
282,503 + Pf1N1B4_5848 0.54 -0.2
282,503 + Pf1N1B4_5848 0.54 -0.4
282,503 + Pf1N1B4_5848 0.54 +2.2
282,503 + Pf1N1B4_5848 0.54 -0.4
282,504 - Pf1N1B4_5848 0.54 -0.0
282,504 - Pf1N1B4_5848 0.54 -0.4
282,623 + +1.0
282,692 + Pf1N1B4_5848 0.79 -2.0
282,692 + Pf1N1B4_5848 0.79 -0.6
282,693 - Pf1N1B4_5848 0.79 +0.3
282,888 + -0.8
282,889 - +0.4
283,020 - -0.6
283,020 - Pf1N1B4_5849 0.35 -1.3
283,530 + +1.0
283,530 + +1.0
283,531 - -0.6
283,531 - -0.0
283,683 + -1.2
283,683 + +2.3
283,801 + +1.1
283,824 + -0.1
283,825 - +0.4
284,036 - Pf1N1B4_5851 0.33 -2.1
284,058 - Pf1N1B4_5851 0.35 -2.3
284,058 - Pf1N1B4_5851 0.35 -1.2
284,118 + Pf1N1B4_5851 0.42 +0.4
284,119 - Pf1N1B4_5851 0.42 -0.0
284,119 - Pf1N1B4_5851 0.42 -0.6
284,402 + Pf1N1B4_5851 0.75 +1.0
284,403 - -1.8

Or see this region's nucleotide sequence