Experiment: sodium fluoride 12.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1221 and Pf1N1B4_1222 are separated by 48 nucleotides Pf1N1B4_1222 and Pf1N1B4_1223 are separated by 69 nucleotides
Pf1N1B4_1221 - coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase, at 1,385,159 to 1,386,304
_1221
Pf1N1B4_1222 - Glucose-1-phosphate thymidylyltransferase (EC 2.7.7.24), at 1,386,353 to 1,387,276
_1222
Pf1N1B4_1223 - dTDP-glucose 4,6-dehydratase (EC 4.2.1.46), at 1,387,346 to 1,388,428
_1223
Position (kb)
1386
1387
1388 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1385.354 kb on + strand, within Pf1N1B4_1221 at 1385.354 kb on + strand, within Pf1N1B4_1221 at 1385.355 kb on - strand, within Pf1N1B4_1221 at 1385.355 kb on - strand, within Pf1N1B4_1221 at 1385.355 kb on - strand, within Pf1N1B4_1221 at 1385.355 kb on - strand, within Pf1N1B4_1221 at 1385.355 kb on - strand, within Pf1N1B4_1221 at 1385.441 kb on + strand, within Pf1N1B4_1221 at 1385.501 kb on - strand, within Pf1N1B4_1221 at 1385.533 kb on + strand, within Pf1N1B4_1221 at 1385.732 kb on + strand, within Pf1N1B4_1221 at 1385.732 kb on + strand, within Pf1N1B4_1221 at 1385.732 kb on + strand, within Pf1N1B4_1221 at 1385.733 kb on - strand, within Pf1N1B4_1221 at 1385.762 kb on - strand, within Pf1N1B4_1221 at 1385.872 kb on + strand, within Pf1N1B4_1221 at 1385.872 kb on + strand, within Pf1N1B4_1221 at 1385.873 kb on - strand, within Pf1N1B4_1221 at 1385.921 kb on + strand, within Pf1N1B4_1221 at 1385.921 kb on + strand, within Pf1N1B4_1221 at 1385.921 kb on + strand, within Pf1N1B4_1221 at 1385.921 kb on + strand, within Pf1N1B4_1221 at 1385.922 kb on - strand at 1386.138 kb on + strand, within Pf1N1B4_1221 at 1386.139 kb on - strand, within Pf1N1B4_1221 at 1386.139 kb on - strand, within Pf1N1B4_1221 at 1386.139 kb on - strand, within Pf1N1B4_1221 at 1386.426 kb on + strand at 1386.426 kb on + strand at 1386.427 kb on - strand at 1386.427 kb on - strand at 1386.501 kb on + strand, within Pf1N1B4_1222 at 1386.501 kb on + strand, within Pf1N1B4_1222 at 1386.501 kb on + strand, within Pf1N1B4_1222 at 1386.502 kb on - strand at 1386.502 kb on - strand, within Pf1N1B4_1222 at 1386.502 kb on - strand, within Pf1N1B4_1222 at 1386.657 kb on + strand, within Pf1N1B4_1222 at 1386.657 kb on + strand, within Pf1N1B4_1222 at 1386.741 kb on + strand, within Pf1N1B4_1222 at 1386.742 kb on - strand, within Pf1N1B4_1222 at 1386.742 kb on - strand, within Pf1N1B4_1222 at 1386.747 kb on + strand, within Pf1N1B4_1222 at 1386.747 kb on + strand, within Pf1N1B4_1222 at 1386.747 kb on + strand, within Pf1N1B4_1222 at 1386.747 kb on + strand, within Pf1N1B4_1222 at 1386.747 kb on + strand, within Pf1N1B4_1222 at 1386.747 kb on + strand, within Pf1N1B4_1222 at 1386.747 kb on + strand, within Pf1N1B4_1222 at 1386.748 kb on - strand, within Pf1N1B4_1222 at 1386.748 kb on - strand, within Pf1N1B4_1222 at 1386.748 kb on - strand, within Pf1N1B4_1222 at 1386.748 kb on - strand, within Pf1N1B4_1222 at 1386.748 kb on - strand, within Pf1N1B4_1222 at 1386.748 kb on - strand, within Pf1N1B4_1222 at 1386.748 kb on - strand, within Pf1N1B4_1222 at 1386.861 kb on + strand, within Pf1N1B4_1222 at 1386.862 kb on - strand, within Pf1N1B4_1222 at 1386.862 kb on - strand, within Pf1N1B4_1222 at 1386.862 kb on - strand, within Pf1N1B4_1222 at 1386.862 kb on - strand, within Pf1N1B4_1222 at 1386.864 kb on + strand, within Pf1N1B4_1222 at 1386.962 kb on + strand, within Pf1N1B4_1222 at 1386.962 kb on + strand, within Pf1N1B4_1222 at 1386.962 kb on + strand, within Pf1N1B4_1222 at 1386.962 kb on + strand, within Pf1N1B4_1222 at 1386.962 kb on + strand, within Pf1N1B4_1222 at 1386.962 kb on + strand at 1386.962 kb on + strand, within Pf1N1B4_1222 at 1386.962 kb on + strand, within Pf1N1B4_1222 at 1386.962 kb on + strand, within Pf1N1B4_1222 at 1386.962 kb on + strand, within Pf1N1B4_1222 at 1386.962 kb on + strand, within Pf1N1B4_1222 at 1386.962 kb on + strand, within Pf1N1B4_1222 at 1386.962 kb on + strand, within Pf1N1B4_1222 at 1386.962 kb on + strand, within Pf1N1B4_1222 at 1386.963 kb on - strand, within Pf1N1B4_1222 at 1386.963 kb on - strand, within Pf1N1B4_1222 at 1386.963 kb on - strand, within Pf1N1B4_1222 at 1386.963 kb on - strand, within Pf1N1B4_1222 at 1386.963 kb on - strand, within Pf1N1B4_1222 at 1386.963 kb on - strand, within Pf1N1B4_1222 at 1386.963 kb on - strand, within Pf1N1B4_1222 at 1386.963 kb on - strand at 1386.963 kb on - strand, within Pf1N1B4_1222 at 1386.963 kb on - strand, within Pf1N1B4_1222 at 1386.963 kb on - strand, within Pf1N1B4_1222 at 1386.963 kb on - strand, within Pf1N1B4_1222 at 1386.963 kb on - strand, within Pf1N1B4_1222 at 1386.963 kb on - strand, within Pf1N1B4_1222 at 1386.963 kb on - strand, within Pf1N1B4_1222 at 1386.963 kb on - strand, within Pf1N1B4_1222 at 1386.963 kb on - strand, within Pf1N1B4_1222 at 1386.963 kb on - strand, within Pf1N1B4_1222 at 1386.963 kb on - strand, within Pf1N1B4_1222 at 1387.162 kb on - strand, within Pf1N1B4_1222 at 1387.164 kb on + strand, within Pf1N1B4_1222 at 1387.164 kb on + strand, within Pf1N1B4_1222 at 1387.164 kb on + strand, within Pf1N1B4_1222 at 1387.164 kb on + strand, within Pf1N1B4_1222 at 1387.165 kb on - strand, within Pf1N1B4_1222 at 1387.165 kb on - strand, within Pf1N1B4_1222 at 1387.165 kb on - strand, within Pf1N1B4_1222 at 1387.165 kb on - strand, within Pf1N1B4_1222 at 1387.165 kb on - strand, within Pf1N1B4_1222 at 1387.165 kb on - strand, within Pf1N1B4_1222 at 1387.165 kb on - strand, within Pf1N1B4_1222 at 1387.253 kb on + strand at 1387.266 kb on + strand at 1387.266 kb on + strand at 1387.266 kb on + strand at 1387.441 kb on - strand at 1387.530 kb on + strand, within Pf1N1B4_1223 at 1387.686 kb on + strand, within Pf1N1B4_1223 at 1387.686 kb on + strand, within Pf1N1B4_1223 at 1387.686 kb on + strand, within Pf1N1B4_1223 at 1387.686 kb on + strand, within Pf1N1B4_1223 at 1387.686 kb on + strand, within Pf1N1B4_1223 at 1387.686 kb on + strand, within Pf1N1B4_1223 at 1387.686 kb on + strand, within Pf1N1B4_1223 at 1387.686 kb on + strand, within Pf1N1B4_1223 at 1387.687 kb on - strand, within Pf1N1B4_1223 at 1387.687 kb on - strand, within Pf1N1B4_1223 at 1387.687 kb on - strand, within Pf1N1B4_1223 at 1387.687 kb on - strand, within Pf1N1B4_1223 at 1387.687 kb on - strand, within Pf1N1B4_1223 at 1387.687 kb on - strand, within Pf1N1B4_1223 at 1387.687 kb on - strand, within Pf1N1B4_1223 at 1387.722 kb on + strand, within Pf1N1B4_1223 at 1387.722 kb on + strand, within Pf1N1B4_1223 at 1387.722 kb on + strand, within Pf1N1B4_1223 at 1387.722 kb on + strand, within Pf1N1B4_1223 at 1387.722 kb on + strand, within Pf1N1B4_1223 at 1387.723 kb on - strand, within Pf1N1B4_1223 at 1387.723 kb on - strand, within Pf1N1B4_1223 at 1387.723 kb on - strand, within Pf1N1B4_1223 at 1387.723 kb on - strand, within Pf1N1B4_1223 at 1387.783 kb on - strand, within Pf1N1B4_1223 at 1387.783 kb on - strand, within Pf1N1B4_1223 at 1387.861 kb on - strand, within Pf1N1B4_1223 at 1387.881 kb on - strand, within Pf1N1B4_1223 at 1387.971 kb on + strand, within Pf1N1B4_1223 at 1387.972 kb on - strand, within Pf1N1B4_1223 at 1387.972 kb on - strand, within Pf1N1B4_1223 at 1388.019 kb on + strand, within Pf1N1B4_1223 at 1388.019 kb on + strand, within Pf1N1B4_1223 at 1388.115 kb on + strand, within Pf1N1B4_1223 at 1388.116 kb on - strand, within Pf1N1B4_1223 at 1388.116 kb on - strand, within Pf1N1B4_1223 at 1388.116 kb on - strand, within Pf1N1B4_1223 at 1388.128 kb on + strand, within Pf1N1B4_1223
Per-strain Table
Position Strand Gene LocusTag Fraction sodium fluoride 12.5 mM remove 1,385,354 + Pf1N1B4_1221 0.17 -0.2 1,385,354 + Pf1N1B4_1221 0.17 +0.8 1,385,355 - Pf1N1B4_1221 0.17 +1.5 1,385,355 - Pf1N1B4_1221 0.17 +0.5 1,385,355 - Pf1N1B4_1221 0.17 -2.0 1,385,355 - Pf1N1B4_1221 0.17 -0.5 1,385,355 - Pf1N1B4_1221 0.17 -1.3 1,385,441 + Pf1N1B4_1221 0.25 -1.3 1,385,501 - Pf1N1B4_1221 0.30 +1.0 1,385,533 + Pf1N1B4_1221 0.33 -0.8 1,385,732 + Pf1N1B4_1221 0.50 -0.2 1,385,732 + Pf1N1B4_1221 0.50 -0.6 1,385,732 + Pf1N1B4_1221 0.50 -1.0 1,385,733 - Pf1N1B4_1221 0.50 -0.3 1,385,762 - Pf1N1B4_1221 0.53 -1.5 1,385,872 + Pf1N1B4_1221 0.62 +0.1 1,385,872 + Pf1N1B4_1221 0.62 -0.5 1,385,873 - Pf1N1B4_1221 0.62 -1.9 1,385,921 + Pf1N1B4_1221 0.66 -0.0 1,385,921 + Pf1N1B4_1221 0.66 -0.5 1,385,921 + Pf1N1B4_1221 0.66 -0.0 1,385,921 + Pf1N1B4_1221 0.66 -0.5 1,385,922 - +0.5 1,386,138 + Pf1N1B4_1221 0.85 +0.4 1,386,139 - Pf1N1B4_1221 0.86 -0.8 1,386,139 - Pf1N1B4_1221 0.86 -0.0 1,386,139 - Pf1N1B4_1221 0.86 -1.5 1,386,426 + -1.0 1,386,426 + +1.1 1,386,427 - +2.0 1,386,427 - +0.2 1,386,501 + Pf1N1B4_1222 0.16 +1.2 1,386,501 + Pf1N1B4_1222 0.16 +0.4 1,386,501 + Pf1N1B4_1222 0.16 +0.8 1,386,502 - +2.5 1,386,502 - Pf1N1B4_1222 0.16 +1.3 1,386,502 - Pf1N1B4_1222 0.16 +1.3 1,386,657 + Pf1N1B4_1222 0.33 -0.8 1,386,657 + Pf1N1B4_1222 0.33 -0.3 1,386,741 + Pf1N1B4_1222 0.42 -1.6 1,386,742 - Pf1N1B4_1222 0.42 +1.5 1,386,742 - Pf1N1B4_1222 0.42 -1.0 1,386,747 + Pf1N1B4_1222 0.43 -0.0 1,386,747 + Pf1N1B4_1222 0.43 -0.5 1,386,747 + Pf1N1B4_1222 0.43 -0.0 1,386,747 + Pf1N1B4_1222 0.43 +0.2 1,386,747 + Pf1N1B4_1222 0.43 -1.8 1,386,747 + Pf1N1B4_1222 0.43 -0.8 1,386,747 + Pf1N1B4_1222 0.43 -0.6 1,386,748 - Pf1N1B4_1222 0.43 +0.5 1,386,748 - Pf1N1B4_1222 0.43 -1.3 1,386,748 - Pf1N1B4_1222 0.43 -0.2 1,386,748 - Pf1N1B4_1222 0.43 +0.9 1,386,748 - Pf1N1B4_1222 0.43 -0.0 1,386,748 - Pf1N1B4_1222 0.43 -0.3 1,386,748 - Pf1N1B4_1222 0.43 -1.0 1,386,861 + Pf1N1B4_1222 0.55 -1.6 1,386,862 - Pf1N1B4_1222 0.55 -1.2 1,386,862 - Pf1N1B4_1222 0.55 -1.5 1,386,862 - Pf1N1B4_1222 0.55 -0.4 1,386,862 - Pf1N1B4_1222 0.55 +1.5 1,386,864 + Pf1N1B4_1222 0.55 -0.0 1,386,962 + Pf1N1B4_1222 0.66 +1.3 1,386,962 + Pf1N1B4_1222 0.66 +0.7 1,386,962 + Pf1N1B4_1222 0.66 +0.2 1,386,962 + Pf1N1B4_1222 0.66 +0.0 1,386,962 + Pf1N1B4_1222 0.66 +0.5 1,386,962 + +2.5 1,386,962 + Pf1N1B4_1222 0.66 +0.4 1,386,962 + Pf1N1B4_1222 0.66 -0.0 1,386,962 + Pf1N1B4_1222 0.66 +1.1 1,386,962 + Pf1N1B4_1222 0.66 +1.2 1,386,962 + Pf1N1B4_1222 0.66 -0.0 1,386,962 + Pf1N1B4_1222 0.66 -0.3 1,386,962 + Pf1N1B4_1222 0.66 +2.0 1,386,962 + Pf1N1B4_1222 0.66 -0.5 1,386,963 - Pf1N1B4_1222 0.66 +0.4 1,386,963 - Pf1N1B4_1222 0.66 -1.6 1,386,963 - Pf1N1B4_1222 0.66 +0.5 1,386,963 - Pf1N1B4_1222 0.66 -1.0 1,386,963 - Pf1N1B4_1222 0.66 +1.5 1,386,963 - Pf1N1B4_1222 0.66 +0.5 1,386,963 - Pf1N1B4_1222 0.66 -0.0 1,386,963 - -0.5 1,386,963 - Pf1N1B4_1222 0.66 -0.7 1,386,963 - Pf1N1B4_1222 0.66 +1.1 1,386,963 - Pf1N1B4_1222 0.66 +1.9 1,386,963 - Pf1N1B4_1222 0.66 -1.5 1,386,963 - Pf1N1B4_1222 0.66 -1.2 1,386,963 - Pf1N1B4_1222 0.66 -0.0 1,386,963 - Pf1N1B4_1222 0.66 -0.6 1,386,963 - Pf1N1B4_1222 0.66 -1.5 1,386,963 - Pf1N1B4_1222 0.66 +0.3 1,386,963 - Pf1N1B4_1222 0.66 -1.0 1,386,963 - Pf1N1B4_1222 0.66 +1.5 1,387,162 - Pf1N1B4_1222 0.88 +1.4 1,387,164 + Pf1N1B4_1222 0.88 +0.2 1,387,164 + Pf1N1B4_1222 0.88 +0.2 1,387,164 + Pf1N1B4_1222 0.88 -0.1 1,387,164 + Pf1N1B4_1222 0.88 +1.0 1,387,165 - Pf1N1B4_1222 0.88 +0.5 1,387,165 - Pf1N1B4_1222 0.88 +0.5 1,387,165 - Pf1N1B4_1222 0.88 -1.9 1,387,165 - Pf1N1B4_1222 0.88 +0.1 1,387,165 - Pf1N1B4_1222 0.88 +1.0 1,387,165 - Pf1N1B4_1222 0.88 +0.2 1,387,165 - Pf1N1B4_1222 0.88 +0.5 1,387,253 + -0.0 1,387,266 + +1.5 1,387,266 + -0.7 1,387,266 + -0.8 1,387,441 - -0.8 1,387,530 + Pf1N1B4_1223 0.17 +0.5 1,387,686 + Pf1N1B4_1223 0.31 -1.5 1,387,686 + Pf1N1B4_1223 0.31 -0.2 1,387,686 + Pf1N1B4_1223 0.31 +0.5 1,387,686 + Pf1N1B4_1223 0.31 -0.5 1,387,686 + Pf1N1B4_1223 0.31 -1.3 1,387,686 + Pf1N1B4_1223 0.31 -0.5 1,387,686 + Pf1N1B4_1223 0.31 +0.7 1,387,686 + Pf1N1B4_1223 0.31 +0.4 1,387,687 - Pf1N1B4_1223 0.31 +0.1 1,387,687 - Pf1N1B4_1223 0.31 +0.5 1,387,687 - Pf1N1B4_1223 0.31 +0.4 1,387,687 - Pf1N1B4_1223 0.31 +1.9 1,387,687 - Pf1N1B4_1223 0.31 +0.3 1,387,687 - Pf1N1B4_1223 0.31 -1.4 1,387,687 - Pf1N1B4_1223 0.31 +0.5 1,387,722 + Pf1N1B4_1223 0.35 -0.3 1,387,722 + Pf1N1B4_1223 0.35 +0.7 1,387,722 + Pf1N1B4_1223 0.35 +0.5 1,387,722 + Pf1N1B4_1223 0.35 +0.2 1,387,722 + Pf1N1B4_1223 0.35 +1.2 1,387,723 - Pf1N1B4_1223 0.35 -1.8 1,387,723 - Pf1N1B4_1223 0.35 -0.0 1,387,723 - Pf1N1B4_1223 0.35 -0.8 1,387,723 - Pf1N1B4_1223 0.35 +0.3 1,387,783 - Pf1N1B4_1223 0.40 +0.9 1,387,783 - Pf1N1B4_1223 0.40 -0.6 1,387,861 - Pf1N1B4_1223 0.48 -0.0 1,387,881 - Pf1N1B4_1223 0.49 -0.9 1,387,971 + Pf1N1B4_1223 0.58 -1.3 1,387,972 - Pf1N1B4_1223 0.58 -0.3 1,387,972 - Pf1N1B4_1223 0.58 +0.4 1,388,019 + Pf1N1B4_1223 0.62 +0.4 1,388,019 + Pf1N1B4_1223 0.62 +0.8 1,388,115 + Pf1N1B4_1223 0.71 -1.9 1,388,116 - Pf1N1B4_1223 0.71 -0.8 1,388,116 - Pf1N1B4_1223 0.71 +1.7 1,388,116 - Pf1N1B4_1223 0.71 +1.0 1,388,128 + Pf1N1B4_1223 0.72 -0.5
Or see this region's nucleotide sequence