Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_1222

Experiment: sodium fluoride 12.5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_1221 and Pf1N1B4_1222 are separated by 48 nucleotidesPf1N1B4_1222 and Pf1N1B4_1223 are separated by 69 nucleotides Pf1N1B4_1221 - coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase, at 1,385,159 to 1,386,304 _1221 Pf1N1B4_1222 - Glucose-1-phosphate thymidylyltransferase (EC 2.7.7.24), at 1,386,353 to 1,387,276 _1222 Pf1N1B4_1223 - dTDP-glucose 4,6-dehydratase (EC 4.2.1.46), at 1,387,346 to 1,388,428 _1223 Position (kb) 1386 1387 1388Strain fitness (log2 ratio) -2 -1 0 1 2at 1385.354 kb on + strand, within Pf1N1B4_1221at 1385.354 kb on + strand, within Pf1N1B4_1221at 1385.355 kb on - strand, within Pf1N1B4_1221at 1385.355 kb on - strand, within Pf1N1B4_1221at 1385.355 kb on - strand, within Pf1N1B4_1221at 1385.355 kb on - strand, within Pf1N1B4_1221at 1385.355 kb on - strand, within Pf1N1B4_1221at 1385.441 kb on + strand, within Pf1N1B4_1221at 1385.501 kb on - strand, within Pf1N1B4_1221at 1385.533 kb on + strand, within Pf1N1B4_1221at 1385.732 kb on + strand, within Pf1N1B4_1221at 1385.732 kb on + strand, within Pf1N1B4_1221at 1385.732 kb on + strand, within Pf1N1B4_1221at 1385.733 kb on - strand, within Pf1N1B4_1221at 1385.762 kb on - strand, within Pf1N1B4_1221at 1385.872 kb on + strand, within Pf1N1B4_1221at 1385.872 kb on + strand, within Pf1N1B4_1221at 1385.873 kb on - strand, within Pf1N1B4_1221at 1385.921 kb on + strand, within Pf1N1B4_1221at 1385.921 kb on + strand, within Pf1N1B4_1221at 1385.921 kb on + strand, within Pf1N1B4_1221at 1385.921 kb on + strand, within Pf1N1B4_1221at 1385.922 kb on - strandat 1386.138 kb on + strand, within Pf1N1B4_1221at 1386.139 kb on - strand, within Pf1N1B4_1221at 1386.139 kb on - strand, within Pf1N1B4_1221at 1386.139 kb on - strand, within Pf1N1B4_1221at 1386.426 kb on + strandat 1386.426 kb on + strandat 1386.427 kb on - strandat 1386.427 kb on - strandat 1386.501 kb on + strand, within Pf1N1B4_1222at 1386.501 kb on + strand, within Pf1N1B4_1222at 1386.501 kb on + strand, within Pf1N1B4_1222at 1386.502 kb on - strandat 1386.502 kb on - strand, within Pf1N1B4_1222at 1386.502 kb on - strand, within Pf1N1B4_1222at 1386.657 kb on + strand, within Pf1N1B4_1222at 1386.657 kb on + strand, within Pf1N1B4_1222at 1386.741 kb on + strand, within Pf1N1B4_1222at 1386.742 kb on - strand, within Pf1N1B4_1222at 1386.742 kb on - strand, within Pf1N1B4_1222at 1386.747 kb on + strand, within Pf1N1B4_1222at 1386.747 kb on + strand, within Pf1N1B4_1222at 1386.747 kb on + strand, within Pf1N1B4_1222at 1386.747 kb on + strand, within Pf1N1B4_1222at 1386.747 kb on + strand, within Pf1N1B4_1222at 1386.747 kb on + strand, within Pf1N1B4_1222at 1386.747 kb on + strand, within Pf1N1B4_1222at 1386.748 kb on - strand, within Pf1N1B4_1222at 1386.748 kb on - strand, within Pf1N1B4_1222at 1386.748 kb on - strand, within Pf1N1B4_1222at 1386.748 kb on - strand, within Pf1N1B4_1222at 1386.748 kb on - strand, within Pf1N1B4_1222at 1386.748 kb on - strand, within Pf1N1B4_1222at 1386.748 kb on - strand, within Pf1N1B4_1222at 1386.861 kb on + strand, within Pf1N1B4_1222at 1386.862 kb on - strand, within Pf1N1B4_1222at 1386.862 kb on - strand, within Pf1N1B4_1222at 1386.862 kb on - strand, within Pf1N1B4_1222at 1386.862 kb on - strand, within Pf1N1B4_1222at 1386.864 kb on + strand, within Pf1N1B4_1222at 1386.962 kb on + strand, within Pf1N1B4_1222at 1386.962 kb on + strand, within Pf1N1B4_1222at 1386.962 kb on + strand, within Pf1N1B4_1222at 1386.962 kb on + strand, within Pf1N1B4_1222at 1386.962 kb on + strand, within Pf1N1B4_1222at 1386.962 kb on + strandat 1386.962 kb on + strand, within Pf1N1B4_1222at 1386.962 kb on + strand, within Pf1N1B4_1222at 1386.962 kb on + strand, within Pf1N1B4_1222at 1386.962 kb on + strand, within Pf1N1B4_1222at 1386.962 kb on + strand, within Pf1N1B4_1222at 1386.962 kb on + strand, within Pf1N1B4_1222at 1386.962 kb on + strand, within Pf1N1B4_1222at 1386.962 kb on + strand, within Pf1N1B4_1222at 1386.963 kb on - strand, within Pf1N1B4_1222at 1386.963 kb on - strand, within Pf1N1B4_1222at 1386.963 kb on - strand, within Pf1N1B4_1222at 1386.963 kb on - strand, within Pf1N1B4_1222at 1386.963 kb on - strand, within Pf1N1B4_1222at 1386.963 kb on - strand, within Pf1N1B4_1222at 1386.963 kb on - strand, within Pf1N1B4_1222at 1386.963 kb on - strandat 1386.963 kb on - strand, within Pf1N1B4_1222at 1386.963 kb on - strand, within Pf1N1B4_1222at 1386.963 kb on - strand, within Pf1N1B4_1222at 1386.963 kb on - strand, within Pf1N1B4_1222at 1386.963 kb on - strand, within Pf1N1B4_1222at 1386.963 kb on - strand, within Pf1N1B4_1222at 1386.963 kb on - strand, within Pf1N1B4_1222at 1386.963 kb on - strand, within Pf1N1B4_1222at 1386.963 kb on - strand, within Pf1N1B4_1222at 1386.963 kb on - strand, within Pf1N1B4_1222at 1386.963 kb on - strand, within Pf1N1B4_1222at 1387.162 kb on - strand, within Pf1N1B4_1222at 1387.164 kb on + strand, within Pf1N1B4_1222at 1387.164 kb on + strand, within Pf1N1B4_1222at 1387.164 kb on + strand, within Pf1N1B4_1222at 1387.164 kb on + strand, within Pf1N1B4_1222at 1387.165 kb on - strand, within Pf1N1B4_1222at 1387.165 kb on - strand, within Pf1N1B4_1222at 1387.165 kb on - strand, within Pf1N1B4_1222at 1387.165 kb on - strand, within Pf1N1B4_1222at 1387.165 kb on - strand, within Pf1N1B4_1222at 1387.165 kb on - strand, within Pf1N1B4_1222at 1387.165 kb on - strand, within Pf1N1B4_1222at 1387.253 kb on + strandat 1387.266 kb on + strandat 1387.266 kb on + strandat 1387.266 kb on + strandat 1387.441 kb on - strandat 1387.530 kb on + strand, within Pf1N1B4_1223at 1387.686 kb on + strand, within Pf1N1B4_1223at 1387.686 kb on + strand, within Pf1N1B4_1223at 1387.686 kb on + strand, within Pf1N1B4_1223at 1387.686 kb on + strand, within Pf1N1B4_1223at 1387.686 kb on + strand, within Pf1N1B4_1223at 1387.686 kb on + strand, within Pf1N1B4_1223at 1387.686 kb on + strand, within Pf1N1B4_1223at 1387.686 kb on + strand, within Pf1N1B4_1223at 1387.687 kb on - strand, within Pf1N1B4_1223at 1387.687 kb on - strand, within Pf1N1B4_1223at 1387.687 kb on - strand, within Pf1N1B4_1223at 1387.687 kb on - strand, within Pf1N1B4_1223at 1387.687 kb on - strand, within Pf1N1B4_1223at 1387.687 kb on - strand, within Pf1N1B4_1223at 1387.687 kb on - strand, within Pf1N1B4_1223at 1387.722 kb on + strand, within Pf1N1B4_1223at 1387.722 kb on + strand, within Pf1N1B4_1223at 1387.722 kb on + strand, within Pf1N1B4_1223at 1387.722 kb on + strand, within Pf1N1B4_1223at 1387.722 kb on + strand, within Pf1N1B4_1223at 1387.723 kb on - strand, within Pf1N1B4_1223at 1387.723 kb on - strand, within Pf1N1B4_1223at 1387.723 kb on - strand, within Pf1N1B4_1223at 1387.723 kb on - strand, within Pf1N1B4_1223at 1387.783 kb on - strand, within Pf1N1B4_1223at 1387.783 kb on - strand, within Pf1N1B4_1223at 1387.861 kb on - strand, within Pf1N1B4_1223at 1387.881 kb on - strand, within Pf1N1B4_1223at 1387.971 kb on + strand, within Pf1N1B4_1223at 1387.972 kb on - strand, within Pf1N1B4_1223at 1387.972 kb on - strand, within Pf1N1B4_1223at 1388.019 kb on + strand, within Pf1N1B4_1223at 1388.019 kb on + strand, within Pf1N1B4_1223at 1388.115 kb on + strand, within Pf1N1B4_1223at 1388.116 kb on - strand, within Pf1N1B4_1223at 1388.116 kb on - strand, within Pf1N1B4_1223at 1388.116 kb on - strand, within Pf1N1B4_1223at 1388.128 kb on + strand, within Pf1N1B4_1223

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Per-strain Table

Position Strand Gene LocusTag Fraction sodium fluoride 12.5 mM
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1,385,354 + Pf1N1B4_1221 0.17 -0.2
1,385,354 + Pf1N1B4_1221 0.17 +0.8
1,385,355 - Pf1N1B4_1221 0.17 +1.5
1,385,355 - Pf1N1B4_1221 0.17 +0.5
1,385,355 - Pf1N1B4_1221 0.17 -2.0
1,385,355 - Pf1N1B4_1221 0.17 -0.5
1,385,355 - Pf1N1B4_1221 0.17 -1.3
1,385,441 + Pf1N1B4_1221 0.25 -1.3
1,385,501 - Pf1N1B4_1221 0.30 +1.0
1,385,533 + Pf1N1B4_1221 0.33 -0.8
1,385,732 + Pf1N1B4_1221 0.50 -0.2
1,385,732 + Pf1N1B4_1221 0.50 -0.6
1,385,732 + Pf1N1B4_1221 0.50 -1.0
1,385,733 - Pf1N1B4_1221 0.50 -0.3
1,385,762 - Pf1N1B4_1221 0.53 -1.5
1,385,872 + Pf1N1B4_1221 0.62 +0.1
1,385,872 + Pf1N1B4_1221 0.62 -0.5
1,385,873 - Pf1N1B4_1221 0.62 -1.9
1,385,921 + Pf1N1B4_1221 0.66 -0.0
1,385,921 + Pf1N1B4_1221 0.66 -0.5
1,385,921 + Pf1N1B4_1221 0.66 -0.0
1,385,921 + Pf1N1B4_1221 0.66 -0.5
1,385,922 - +0.5
1,386,138 + Pf1N1B4_1221 0.85 +0.4
1,386,139 - Pf1N1B4_1221 0.86 -0.8
1,386,139 - Pf1N1B4_1221 0.86 -0.0
1,386,139 - Pf1N1B4_1221 0.86 -1.5
1,386,426 + -1.0
1,386,426 + +1.1
1,386,427 - +2.0
1,386,427 - +0.2
1,386,501 + Pf1N1B4_1222 0.16 +1.2
1,386,501 + Pf1N1B4_1222 0.16 +0.4
1,386,501 + Pf1N1B4_1222 0.16 +0.8
1,386,502 - +2.5
1,386,502 - Pf1N1B4_1222 0.16 +1.3
1,386,502 - Pf1N1B4_1222 0.16 +1.3
1,386,657 + Pf1N1B4_1222 0.33 -0.8
1,386,657 + Pf1N1B4_1222 0.33 -0.3
1,386,741 + Pf1N1B4_1222 0.42 -1.6
1,386,742 - Pf1N1B4_1222 0.42 +1.5
1,386,742 - Pf1N1B4_1222 0.42 -1.0
1,386,747 + Pf1N1B4_1222 0.43 -0.0
1,386,747 + Pf1N1B4_1222 0.43 -0.5
1,386,747 + Pf1N1B4_1222 0.43 -0.0
1,386,747 + Pf1N1B4_1222 0.43 +0.2
1,386,747 + Pf1N1B4_1222 0.43 -1.8
1,386,747 + Pf1N1B4_1222 0.43 -0.8
1,386,747 + Pf1N1B4_1222 0.43 -0.6
1,386,748 - Pf1N1B4_1222 0.43 +0.5
1,386,748 - Pf1N1B4_1222 0.43 -1.3
1,386,748 - Pf1N1B4_1222 0.43 -0.2
1,386,748 - Pf1N1B4_1222 0.43 +0.9
1,386,748 - Pf1N1B4_1222 0.43 -0.0
1,386,748 - Pf1N1B4_1222 0.43 -0.3
1,386,748 - Pf1N1B4_1222 0.43 -1.0
1,386,861 + Pf1N1B4_1222 0.55 -1.6
1,386,862 - Pf1N1B4_1222 0.55 -1.2
1,386,862 - Pf1N1B4_1222 0.55 -1.5
1,386,862 - Pf1N1B4_1222 0.55 -0.4
1,386,862 - Pf1N1B4_1222 0.55 +1.5
1,386,864 + Pf1N1B4_1222 0.55 -0.0
1,386,962 + Pf1N1B4_1222 0.66 +1.3
1,386,962 + Pf1N1B4_1222 0.66 +0.7
1,386,962 + Pf1N1B4_1222 0.66 +0.2
1,386,962 + Pf1N1B4_1222 0.66 +0.0
1,386,962 + Pf1N1B4_1222 0.66 +0.5
1,386,962 + +2.5
1,386,962 + Pf1N1B4_1222 0.66 +0.4
1,386,962 + Pf1N1B4_1222 0.66 -0.0
1,386,962 + Pf1N1B4_1222 0.66 +1.1
1,386,962 + Pf1N1B4_1222 0.66 +1.2
1,386,962 + Pf1N1B4_1222 0.66 -0.0
1,386,962 + Pf1N1B4_1222 0.66 -0.3
1,386,962 + Pf1N1B4_1222 0.66 +2.0
1,386,962 + Pf1N1B4_1222 0.66 -0.5
1,386,963 - Pf1N1B4_1222 0.66 +0.4
1,386,963 - Pf1N1B4_1222 0.66 -1.6
1,386,963 - Pf1N1B4_1222 0.66 +0.5
1,386,963 - Pf1N1B4_1222 0.66 -1.0
1,386,963 - Pf1N1B4_1222 0.66 +1.5
1,386,963 - Pf1N1B4_1222 0.66 +0.5
1,386,963 - Pf1N1B4_1222 0.66 -0.0
1,386,963 - -0.5
1,386,963 - Pf1N1B4_1222 0.66 -0.7
1,386,963 - Pf1N1B4_1222 0.66 +1.1
1,386,963 - Pf1N1B4_1222 0.66 +1.9
1,386,963 - Pf1N1B4_1222 0.66 -1.5
1,386,963 - Pf1N1B4_1222 0.66 -1.2
1,386,963 - Pf1N1B4_1222 0.66 -0.0
1,386,963 - Pf1N1B4_1222 0.66 -0.6
1,386,963 - Pf1N1B4_1222 0.66 -1.5
1,386,963 - Pf1N1B4_1222 0.66 +0.3
1,386,963 - Pf1N1B4_1222 0.66 -1.0
1,386,963 - Pf1N1B4_1222 0.66 +1.5
1,387,162 - Pf1N1B4_1222 0.88 +1.4
1,387,164 + Pf1N1B4_1222 0.88 +0.2
1,387,164 + Pf1N1B4_1222 0.88 +0.2
1,387,164 + Pf1N1B4_1222 0.88 -0.1
1,387,164 + Pf1N1B4_1222 0.88 +1.0
1,387,165 - Pf1N1B4_1222 0.88 +0.5
1,387,165 - Pf1N1B4_1222 0.88 +0.5
1,387,165 - Pf1N1B4_1222 0.88 -1.9
1,387,165 - Pf1N1B4_1222 0.88 +0.1
1,387,165 - Pf1N1B4_1222 0.88 +1.0
1,387,165 - Pf1N1B4_1222 0.88 +0.2
1,387,165 - Pf1N1B4_1222 0.88 +0.5
1,387,253 + -0.0
1,387,266 + +1.5
1,387,266 + -0.7
1,387,266 + -0.8
1,387,441 - -0.8
1,387,530 + Pf1N1B4_1223 0.17 +0.5
1,387,686 + Pf1N1B4_1223 0.31 -1.5
1,387,686 + Pf1N1B4_1223 0.31 -0.2
1,387,686 + Pf1N1B4_1223 0.31 +0.5
1,387,686 + Pf1N1B4_1223 0.31 -0.5
1,387,686 + Pf1N1B4_1223 0.31 -1.3
1,387,686 + Pf1N1B4_1223 0.31 -0.5
1,387,686 + Pf1N1B4_1223 0.31 +0.7
1,387,686 + Pf1N1B4_1223 0.31 +0.4
1,387,687 - Pf1N1B4_1223 0.31 +0.1
1,387,687 - Pf1N1B4_1223 0.31 +0.5
1,387,687 - Pf1N1B4_1223 0.31 +0.4
1,387,687 - Pf1N1B4_1223 0.31 +1.9
1,387,687 - Pf1N1B4_1223 0.31 +0.3
1,387,687 - Pf1N1B4_1223 0.31 -1.4
1,387,687 - Pf1N1B4_1223 0.31 +0.5
1,387,722 + Pf1N1B4_1223 0.35 -0.3
1,387,722 + Pf1N1B4_1223 0.35 +0.7
1,387,722 + Pf1N1B4_1223 0.35 +0.5
1,387,722 + Pf1N1B4_1223 0.35 +0.2
1,387,722 + Pf1N1B4_1223 0.35 +1.2
1,387,723 - Pf1N1B4_1223 0.35 -1.8
1,387,723 - Pf1N1B4_1223 0.35 -0.0
1,387,723 - Pf1N1B4_1223 0.35 -0.8
1,387,723 - Pf1N1B4_1223 0.35 +0.3
1,387,783 - Pf1N1B4_1223 0.40 +0.9
1,387,783 - Pf1N1B4_1223 0.40 -0.6
1,387,861 - Pf1N1B4_1223 0.48 -0.0
1,387,881 - Pf1N1B4_1223 0.49 -0.9
1,387,971 + Pf1N1B4_1223 0.58 -1.3
1,387,972 - Pf1N1B4_1223 0.58 -0.3
1,387,972 - Pf1N1B4_1223 0.58 +0.4
1,388,019 + Pf1N1B4_1223 0.62 +0.4
1,388,019 + Pf1N1B4_1223 0.62 +0.8
1,388,115 + Pf1N1B4_1223 0.71 -1.9
1,388,116 - Pf1N1B4_1223 0.71 -0.8
1,388,116 - Pf1N1B4_1223 0.71 +1.7
1,388,116 - Pf1N1B4_1223 0.71 +1.0
1,388,128 + Pf1N1B4_1223 0.72 -0.5

Or see this region's nucleotide sequence