Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS07345 and MMJJ_RS07350 are separated by 13 nucleotides MMJJ_RS07350 and MMJJ_RS07355 are separated by 44 nucleotides MMJJ_RS07355 and MMJJ_RS07360 are separated by 15 nucleotides MMJJ_RS07360 and MMJJ_RS07365 are separated by 17 nucleotides MMJJ_RS07365 and MMJJ_RS07370 are separated by 15 nucleotides
MMJJ_RS07345: MMJJ_RS07345 - homocysteine biosynthesis protein, at 1,349,284 to 1,350,825
_RS07345
MMJJ_RS07350: MMJJ_RS07350 - 4Fe-4S binding protein, at 1,350,839 to 1,351,237
_RS07350
MMJJ_RS07355: MMJJ_RS07355 - MoaD/ThiS family protein, at 1,351,282 to 1,351,536
_RS07355
MMJJ_RS07360: MMJJ_RS07360 - TIGR00269 family protein, at 1,351,552 to 1,352,487
_RS07360
MMJJ_RS07365: MMJJ_RS07365 - CatB-related O-acetyltransferase, at 1,352,505 to 1,353,185
_RS07365
MMJJ_RS07370: MMJJ_RS07370 - tRNA 4-thiouridine(8) synthase ThiI, at 1,353,201 to 1,354,352
_RS07370
Position (kb)
1351
1352
1353 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1350.652 kb on + strand, within MMJJ_RS07345 at 1350.655 kb on - strand, within MMJJ_RS07345 at 1350.655 kb on - strand, within MMJJ_RS07345 at 1350.655 kb on - strand, within MMJJ_RS07345 at 1350.655 kb on - strand, within MMJJ_RS07345 at 1350.655 kb on - strand at 1350.656 kb on + strand, within MMJJ_RS07345 at 1350.656 kb on + strand, within MMJJ_RS07345 at 1350.656 kb on + strand, within MMJJ_RS07345 at 1350.656 kb on + strand, within MMJJ_RS07345 at 1350.657 kb on - strand, within MMJJ_RS07345 at 1350.657 kb on - strand, within MMJJ_RS07345 at 1350.657 kb on - strand, within MMJJ_RS07345 at 1350.658 kb on + strand, within MMJJ_RS07345 at 1350.658 kb on + strand, within MMJJ_RS07345 at 1350.658 kb on + strand, within MMJJ_RS07345 at 1350.658 kb on + strand, within MMJJ_RS07345 at 1350.659 kb on - strand at 1350.659 kb on - strand, within MMJJ_RS07345 at 1350.660 kb on + strand, within MMJJ_RS07345 at 1350.660 kb on + strand, within MMJJ_RS07345 at 1350.660 kb on + strand, within MMJJ_RS07345 at 1350.660 kb on + strand, within MMJJ_RS07345 at 1350.660 kb on + strand, within MMJJ_RS07345 at 1350.660 kb on + strand, within MMJJ_RS07345 at 1350.662 kb on - strand, within MMJJ_RS07345 at 1350.684 kb on - strand at 1350.685 kb on + strand at 1350.784 kb on + strand at 1350.879 kb on - strand, within MMJJ_RS07350 at 1350.921 kb on - strand, within MMJJ_RS07350 at 1350.927 kb on - strand, within MMJJ_RS07350 at 1350.927 kb on - strand, within MMJJ_RS07350 at 1350.931 kb on - strand, within MMJJ_RS07350 at 1350.932 kb on + strand, within MMJJ_RS07350 at 1350.937 kb on + strand, within MMJJ_RS07350 at 1351.020 kb on - strand, within MMJJ_RS07350 at 1351.200 kb on - strand at 1351.219 kb on - strand at 1351.225 kb on + strand at 1351.236 kb on + strand at 1351.248 kb on + strand at 1351.272 kb on + strand at 1351.289 kb on - strand at 1351.350 kb on + strand, within MMJJ_RS07355 at 1351.355 kb on - strand, within MMJJ_RS07355 at 1351.409 kb on - strand, within MMJJ_RS07355 at 1351.409 kb on - strand, within MMJJ_RS07355 at 1351.443 kb on + strand, within MMJJ_RS07355 at 1351.443 kb on + strand, within MMJJ_RS07355 at 1351.483 kb on + strand, within MMJJ_RS07355 at 1351.484 kb on - strand, within MMJJ_RS07355 at 1351.484 kb on - strand, within MMJJ_RS07355 at 1351.485 kb on + strand, within MMJJ_RS07355 at 1351.485 kb on + strand, within MMJJ_RS07355 at 1351.485 kb on + strand, within MMJJ_RS07355 at 1351.485 kb on + strand, within MMJJ_RS07355 at 1351.487 kb on - strand, within MMJJ_RS07355 at 1351.487 kb on - strand, within MMJJ_RS07355 at 1351.492 kb on + strand, within MMJJ_RS07355 at 1351.512 kb on + strand at 1351.514 kb on + strand at 1351.514 kb on + strand at 1351.514 kb on + strand at 1351.541 kb on + strand at 1351.583 kb on - strand at 1351.584 kb on + strand at 1351.585 kb on - strand at 1351.585 kb on - strand at 1351.617 kb on - strand at 1352.086 kb on - strand, within MMJJ_RS07360 at 1352.192 kb on - strand, within MMJJ_RS07360 at 1352.331 kb on + strand, within MMJJ_RS07360 at 1352.425 kb on + strand at 1352.485 kb on - strand at 1352.485 kb on - strand at 1352.493 kb on - strand at 1352.526 kb on - strand at 1352.526 kb on - strand at 1352.527 kb on + strand at 1352.556 kb on + strand at 1352.646 kb on + strand, within MMJJ_RS07365 at 1352.673 kb on - strand, within MMJJ_RS07365 at 1352.674 kb on + strand, within MMJJ_RS07365 at 1352.674 kb on + strand, within MMJJ_RS07365 at 1352.674 kb on + strand, within MMJJ_RS07365 at 1352.674 kb on + strand, within MMJJ_RS07365 at 1352.678 kb on - strand, within MMJJ_RS07365 at 1352.678 kb on - strand, within MMJJ_RS07365 at 1352.678 kb on - strand, within MMJJ_RS07365 at 1352.678 kb on - strand, within MMJJ_RS07365 at 1352.678 kb on - strand, within MMJJ_RS07365 at 1352.679 kb on + strand, within MMJJ_RS07365 at 1352.679 kb on + strand, within MMJJ_RS07365 at 1352.680 kb on - strand, within MMJJ_RS07365 at 1352.705 kb on - strand, within MMJJ_RS07365 at 1352.705 kb on - strand, within MMJJ_RS07365 at 1352.706 kb on + strand, within MMJJ_RS07365 at 1352.754 kb on + strand, within MMJJ_RS07365 at 1352.760 kb on - strand, within MMJJ_RS07365 at 1352.761 kb on + strand, within MMJJ_RS07365 at 1352.779 kb on + strand, within MMJJ_RS07365 at 1352.839 kb on + strand, within MMJJ_RS07365 at 1352.863 kb on + strand, within MMJJ_RS07365 at 1352.875 kb on - strand, within MMJJ_RS07365 at 1352.875 kb on - strand, within MMJJ_RS07365 at 1352.876 kb on + strand, within MMJJ_RS07365 at 1352.876 kb on + strand, within MMJJ_RS07365 at 1352.894 kb on + strand, within MMJJ_RS07365 at 1352.919 kb on - strand, within MMJJ_RS07365 at 1352.919 kb on - strand, within MMJJ_RS07365 at 1352.920 kb on + strand, within MMJJ_RS07365 at 1352.920 kb on + strand, within MMJJ_RS07365 at 1352.920 kb on + strand, within MMJJ_RS07365 at 1352.961 kb on + strand, within MMJJ_RS07365 at 1352.964 kb on - strand, within MMJJ_RS07365 at 1353.004 kb on - strand, within MMJJ_RS07365 at 1353.004 kb on - strand, within MMJJ_RS07365 at 1353.011 kb on - strand, within MMJJ_RS07365 at 1353.011 kb on - strand, within MMJJ_RS07365 at 1353.011 kb on - strand, within MMJJ_RS07365 at 1353.011 kb on - strand, within MMJJ_RS07365 at 1353.012 kb on + strand, within MMJJ_RS07365 at 1353.053 kb on + strand, within MMJJ_RS07365 at 1353.056 kb on - strand, within MMJJ_RS07365 at 1353.057 kb on + strand, within MMJJ_RS07365 at 1353.057 kb on + strand, within MMJJ_RS07365 at 1353.057 kb on + strand, within MMJJ_RS07365 at 1353.057 kb on + strand, within MMJJ_RS07365 at 1353.124 kb on - strand at 1353.149 kb on - strand at 1353.152 kb on - strand at 1353.161 kb on + strand at 1353.176 kb on - strand at 1353.188 kb on - strand at 1353.188 kb on - strand at 1353.188 kb on - strand at 1353.190 kb on - strand at 1353.197 kb on - strand at 1353.197 kb on - strand at 1353.285 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE remove 1,350,652 + MMJJ_RS07345 0.89 -0.3 1,350,655 - MMJJ_RS07345 0.89 -1.8 1,350,655 - MMJJ_RS07345 0.89 -2.1 1,350,655 - MMJJ_RS07345 0.89 +0.2 1,350,655 - MMJJ_RS07345 0.89 -1.3 1,350,655 - +0.2 1,350,656 + MMJJ_RS07345 0.89 -0.5 1,350,656 + MMJJ_RS07345 0.89 -1.8 1,350,656 + MMJJ_RS07345 0.89 -1.3 1,350,656 + MMJJ_RS07345 0.89 -1.3 1,350,657 - MMJJ_RS07345 0.89 -1.8 1,350,657 - MMJJ_RS07345 0.89 -2.6 1,350,657 - MMJJ_RS07345 0.89 -0.1 1,350,658 + MMJJ_RS07345 0.89 -2.6 1,350,658 + MMJJ_RS07345 0.89 -1.2 1,350,658 + MMJJ_RS07345 0.89 -4.0 1,350,658 + MMJJ_RS07345 0.89 -3.2 1,350,659 - -1.3 1,350,659 - MMJJ_RS07345 0.89 -1.8 1,350,660 + MMJJ_RS07345 0.89 +0.2 1,350,660 + MMJJ_RS07345 0.89 -3.8 1,350,660 + MMJJ_RS07345 0.89 +0.2 1,350,660 + MMJJ_RS07345 0.89 -0.8 1,350,660 + MMJJ_RS07345 0.89 +0.5 1,350,660 + MMJJ_RS07345 0.89 -1.8 1,350,662 - MMJJ_RS07345 0.89 -0.8 1,350,684 - -2.1 1,350,685 + -1.3 1,350,784 + -2.3 1,350,879 - MMJJ_RS07350 0.10 -3.8 1,350,921 - MMJJ_RS07350 0.21 -2.3 1,350,927 - MMJJ_RS07350 0.22 -2.9 1,350,927 - MMJJ_RS07350 0.22 -1.8 1,350,931 - MMJJ_RS07350 0.23 -2.6 1,350,932 + MMJJ_RS07350 0.23 +0.2 1,350,937 + MMJJ_RS07350 0.25 +0.4 1,351,020 - MMJJ_RS07350 0.45 -1.3 1,351,200 - +0.2 1,351,219 - -3.5 1,351,225 + -0.8 1,351,236 + -1.6 1,351,248 + -3.8 1,351,272 + +0.2 1,351,289 - -2.1 1,351,350 + MMJJ_RS07355 0.27 -2.8 1,351,355 - MMJJ_RS07355 0.29 -0.8 1,351,409 - MMJJ_RS07355 0.50 -4.5 1,351,409 - MMJJ_RS07355 0.50 -3.2 1,351,443 + MMJJ_RS07355 0.63 -2.3 1,351,443 + MMJJ_RS07355 0.63 -4.2 1,351,483 + MMJJ_RS07355 0.79 -1.7 1,351,484 - MMJJ_RS07355 0.79 -0.4 1,351,484 - MMJJ_RS07355 0.79 -2.3 1,351,485 + MMJJ_RS07355 0.80 -2.6 1,351,485 + MMJJ_RS07355 0.80 -0.0 1,351,485 + MMJJ_RS07355 0.80 -1.8 1,351,485 + MMJJ_RS07355 0.80 -1.8 1,351,487 - MMJJ_RS07355 0.80 -2.3 1,351,487 - MMJJ_RS07355 0.80 -2.3 1,351,492 + MMJJ_RS07355 0.82 -0.8 1,351,512 + -3.8 1,351,514 + -4.7 1,351,514 + +0.2 1,351,514 + -1.3 1,351,541 + -0.0 1,351,583 - -1.3 1,351,584 + -2.1 1,351,585 - -1.3 1,351,585 - -2.6 1,351,617 - -2.3 1,352,086 - MMJJ_RS07360 0.57 -1.8 1,352,192 - MMJJ_RS07360 0.68 -0.8 1,352,331 + MMJJ_RS07360 0.83 -2.4 1,352,425 + -1.4 1,352,485 - +1.8 1,352,485 - -0.0 1,352,493 - +1.3 1,352,526 - -2.6 1,352,526 - +1.4 1,352,527 + -0.2 1,352,556 + -1.4 1,352,646 + MMJJ_RS07365 0.21 +0.8 1,352,673 - MMJJ_RS07365 0.25 -0.2 1,352,674 + MMJJ_RS07365 0.25 -1.7 1,352,674 + MMJJ_RS07365 0.25 -3.2 1,352,674 + MMJJ_RS07365 0.25 -1.7 1,352,674 + MMJJ_RS07365 0.25 +1.0 1,352,678 - MMJJ_RS07365 0.25 +1.4 1,352,678 - MMJJ_RS07365 0.25 +1.8 1,352,678 - MMJJ_RS07365 0.25 -1.5 1,352,678 - MMJJ_RS07365 0.25 +1.7 1,352,678 - MMJJ_RS07365 0.25 -0.1 1,352,679 + MMJJ_RS07365 0.26 +2.1 1,352,679 + MMJJ_RS07365 0.26 +1.1 1,352,680 - MMJJ_RS07365 0.26 -1.5 1,352,705 - MMJJ_RS07365 0.29 +1.3 1,352,705 - MMJJ_RS07365 0.29 +0.4 1,352,706 + MMJJ_RS07365 0.30 +0.0 1,352,754 + MMJJ_RS07365 0.37 +1.3 1,352,760 - MMJJ_RS07365 0.37 +0.2 1,352,761 + MMJJ_RS07365 0.38 +1.8 1,352,779 + MMJJ_RS07365 0.40 +1.2 1,352,839 + MMJJ_RS07365 0.49 -2.8 1,352,863 + MMJJ_RS07365 0.53 +1.2 1,352,875 - MMJJ_RS07365 0.54 +1.2 1,352,875 - MMJJ_RS07365 0.54 -2.2 1,352,876 + MMJJ_RS07365 0.54 -2.4 1,352,876 + MMJJ_RS07365 0.54 -2.9 1,352,894 + MMJJ_RS07365 0.57 -1.7 1,352,919 - MMJJ_RS07365 0.61 +1.2 1,352,919 - MMJJ_RS07365 0.61 -1.0 1,352,920 + MMJJ_RS07365 0.61 +1.0 1,352,920 + MMJJ_RS07365 0.61 -3.1 1,352,920 + MMJJ_RS07365 0.61 -2.2 1,352,961 + MMJJ_RS07365 0.67 +0.9 1,352,964 - MMJJ_RS07365 0.67 +1.1 1,353,004 - MMJJ_RS07365 0.73 +0.6 1,353,004 - MMJJ_RS07365 0.73 +2.1 1,353,011 - MMJJ_RS07365 0.74 +2.0 1,353,011 - MMJJ_RS07365 0.74 -1.6 1,353,011 - MMJJ_RS07365 0.74 +1.2 1,353,011 - MMJJ_RS07365 0.74 +1.1 1,353,012 + MMJJ_RS07365 0.74 -1.4 1,353,053 + MMJJ_RS07365 0.80 +1.4 1,353,056 - MMJJ_RS07365 0.81 +0.9 1,353,057 + MMJJ_RS07365 0.81 -2.2 1,353,057 + MMJJ_RS07365 0.81 -2.0 1,353,057 + MMJJ_RS07365 0.81 -1.5 1,353,057 + MMJJ_RS07365 0.81 +0.7 1,353,124 - -2.1 1,353,149 - +1.8 1,353,152 - +0.7 1,353,161 + -0.4 1,353,176 - +1.7 1,353,188 - +0.9 1,353,188 - +1.5 1,353,188 - -0.7 1,353,190 - -0.8 1,353,197 - +1.2 1,353,197 - -0.2 1,353,285 - +0.2
Or see this region's nucleotide sequence