Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS05340 and MMJJ_RS05345 are separated by 179 nucleotides MMJJ_RS05345 and MMJJ_RS05350 overlap by 4 nucleotides MMJJ_RS05350 and MMJJ_RS05355 are separated by 4 nucleotides
MMJJ_RS05340: MMJJ_RS05340 - AAA family ATPase, at 992,781 to 993,896
_RS05340
MMJJ_RS05345: MMJJ_RS05345 - ATP-dependent Clp protease proteolytic subunit, at 994,076 to 994,900
_RS05345
MMJJ_RS05350: MMJJ_RS05350 - DUF192 domain-containing protein, at 994,897 to 995,232
_RS05350
MMJJ_RS05355: MMJJ_RS05355 - DUF2121 domain-containing protein, at 995,237 to 996,112
_RS05355
Position (kb) 994
995 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1
2
3 at 993.152 kb on - strand, within MMJJ_RS05340 at 993.165 kb on - strand, within MMJJ_RS05340 at 993.168 kb on - strand, within MMJJ_RS05340 at 993.177 kb on - strand, within MMJJ_RS05340 at 993.177 kb on - strand, within MMJJ_RS05340 at 993.178 kb on + strand, within MMJJ_RS05340 at 993.178 kb on + strand, within MMJJ_RS05340 at 993.236 kb on - strand, within MMJJ_RS05340 at 993.240 kb on - strand, within MMJJ_RS05340 at 993.240 kb on - strand, within MMJJ_RS05340 at 993.255 kb on + strand, within MMJJ_RS05340 at 993.320 kb on - strand, within MMJJ_RS05340 at 993.339 kb on - strand, within MMJJ_RS05340 at 993.339 kb on - strand, within MMJJ_RS05340 at 993.340 kb on + strand, within MMJJ_RS05340 at 993.340 kb on + strand, within MMJJ_RS05340 at 993.352 kb on + strand, within MMJJ_RS05340 at 993.418 kb on + strand, within MMJJ_RS05340 at 993.418 kb on + strand, within MMJJ_RS05340 at 993.447 kb on - strand, within MMJJ_RS05340 at 993.466 kb on - strand, within MMJJ_RS05340 at 993.466 kb on - strand, within MMJJ_RS05340 at 993.471 kb on - strand, within MMJJ_RS05340 at 993.561 kb on + strand, within MMJJ_RS05340 at 993.579 kb on + strand, within MMJJ_RS05340 at 993.583 kb on + strand, within MMJJ_RS05340 at 993.635 kb on - strand, within MMJJ_RS05340 at 993.636 kb on + strand, within MMJJ_RS05340 at 993.636 kb on + strand, within MMJJ_RS05340 at 993.636 kb on + strand, within MMJJ_RS05340 at 993.670 kb on + strand, within MMJJ_RS05340 at 993.670 kb on + strand, within MMJJ_RS05340 at 993.673 kb on - strand, within MMJJ_RS05340 at 993.673 kb on - strand, within MMJJ_RS05340 at 993.674 kb on + strand, within MMJJ_RS05340 at 993.718 kb on - strand, within MMJJ_RS05340 at 993.725 kb on - strand, within MMJJ_RS05340 at 993.726 kb on + strand, within MMJJ_RS05340 at 993.729 kb on - strand, within MMJJ_RS05340 at 993.744 kb on - strand, within MMJJ_RS05340 at 993.744 kb on - strand, within MMJJ_RS05340 at 993.750 kb on + strand, within MMJJ_RS05340 at 993.809 kb on + strand at 993.809 kb on + strand at 993.888 kb on + strand at 993.913 kb on - strand at 993.913 kb on - strand at 993.914 kb on + strand at 993.914 kb on + strand at 993.914 kb on + strand at 993.916 kb on + strand at 993.922 kb on + strand at 993.936 kb on + strand at 993.954 kb on - strand at 993.958 kb on + strand at 993.962 kb on + strand at 993.962 kb on + strand at 993.971 kb on - strand at 993.971 kb on - strand at 993.971 kb on - strand at 993.971 kb on - strand at 993.971 kb on - strand at 993.971 kb on - strand at 993.971 kb on - strand at 993.972 kb on + strand at 993.972 kb on + strand at 993.972 kb on + strand at 993.975 kb on - strand at 993.975 kb on - strand at 993.975 kb on - strand at 993.975 kb on - strand at 993.975 kb on - strand at 993.975 kb on - strand at 993.976 kb on + strand at 993.976 kb on + strand at 993.976 kb on + strand at 993.980 kb on - strand at 993.985 kb on - strand at 993.989 kb on + strand at 993.999 kb on - strand at 993.999 kb on - strand at 994.000 kb on + strand at 994.000 kb on + strand at 994.000 kb on + strand at 994.001 kb on - strand at 994.001 kb on - strand at 994.001 kb on - strand at 994.001 kb on - strand at 994.001 kb on - strand at 994.001 kb on - strand at 994.002 kb on + strand at 994.002 kb on + strand at 994.002 kb on + strand at 994.002 kb on + strand at 994.002 kb on + strand at 994.003 kb on - strand at 994.003 kb on - strand at 994.003 kb on - strand at 994.003 kb on - strand at 994.003 kb on - strand at 994.003 kb on - strand at 994.004 kb on + strand at 994.004 kb on + strand at 994.004 kb on + strand at 994.004 kb on + strand at 994.004 kb on + strand at 994.004 kb on + strand at 994.004 kb on + strand at 994.004 kb on + strand at 994.004 kb on + strand at 994.004 kb on + strand at 994.004 kb on + strand at 994.005 kb on - strand at 994.005 kb on - strand at 994.005 kb on - strand at 994.005 kb on - strand at 994.005 kb on - strand at 994.005 kb on - strand at 994.005 kb on - strand at 994.005 kb on - strand at 994.005 kb on - strand at 994.005 kb on - strand at 994.006 kb on + strand at 994.006 kb on + strand at 994.006 kb on + strand at 994.006 kb on + strand at 994.006 kb on + strand at 994.006 kb on + strand at 994.006 kb on + strand at 994.006 kb on + strand at 994.006 kb on + strand at 994.006 kb on + strand at 994.006 kb on + strand at 994.011 kb on + strand at 994.011 kb on + strand at 994.015 kb on - strand at 994.015 kb on - strand at 994.015 kb on - strand at 994.015 kb on - strand at 994.016 kb on + strand at 994.020 kb on + strand at 994.020 kb on + strand at 994.028 kb on - strand at 994.029 kb on + strand at 994.029 kb on + strand at 994.141 kb on - strand at 994.142 kb on + strand at 994.145 kb on - strand at 994.146 kb on + strand at 994.147 kb on - strand at 994.147 kb on - strand at 994.147 kb on - strand at 994.147 kb on - strand at 994.147 kb on - strand at 994.148 kb on + strand at 994.148 kb on + strand at 994.148 kb on + strand at 994.148 kb on + strand at 994.148 kb on + strand at 994.156 kb on + strand at 994.174 kb on + strand, within MMJJ_RS05345 at 994.259 kb on - strand, within MMJJ_RS05345 at 994.261 kb on - strand, within MMJJ_RS05345 at 994.262 kb on + strand, within MMJJ_RS05345 at 994.262 kb on + strand, within MMJJ_RS05345 at 994.265 kb on - strand, within MMJJ_RS05345 at 994.265 kb on - strand, within MMJJ_RS05345 at 994.265 kb on - strand, within MMJJ_RS05345 at 994.265 kb on - strand, within MMJJ_RS05345 at 994.266 kb on + strand, within MMJJ_RS05345 at 994.266 kb on + strand, within MMJJ_RS05345 at 994.266 kb on + strand, within MMJJ_RS05345 at 994.266 kb on + strand, within MMJJ_RS05345 at 994.266 kb on + strand, within MMJJ_RS05345 at 994.266 kb on + strand, within MMJJ_RS05345 at 994.266 kb on + strand, within MMJJ_RS05345 at 994.266 kb on + strand, within MMJJ_RS05345 at 994.266 kb on - strand, within MMJJ_RS05345 at 994.267 kb on - strand, within MMJJ_RS05345 at 994.268 kb on + strand, within MMJJ_RS05345 at 994.270 kb on - strand, within MMJJ_RS05345 at 994.275 kb on - strand, within MMJJ_RS05345 at 994.306 kb on - strand, within MMJJ_RS05345 at 994.306 kb on - strand, within MMJJ_RS05345 at 994.339 kb on + strand, within MMJJ_RS05345 at 994.339 kb on + strand, within MMJJ_RS05345 at 994.339 kb on + strand, within MMJJ_RS05345 at 994.342 kb on + strand, within MMJJ_RS05345 at 994.366 kb on + strand, within MMJJ_RS05345 at 994.366 kb on + strand, within MMJJ_RS05345 at 994.424 kb on + strand, within MMJJ_RS05345 at 994.424 kb on + strand, within MMJJ_RS05345 at 994.461 kb on - strand, within MMJJ_RS05345 at 994.486 kb on + strand, within MMJJ_RS05345 at 994.632 kb on - strand, within MMJJ_RS05345 at 994.655 kb on - strand, within MMJJ_RS05345 at 994.656 kb on + strand, within MMJJ_RS05345 at 994.660 kb on - strand, within MMJJ_RS05345 at 994.660 kb on - strand, within MMJJ_RS05345 at 994.661 kb on + strand, within MMJJ_RS05345 at 994.786 kb on - strand, within MMJJ_RS05345 at 994.862 kb on - strand at 994.863 kb on + strand at 994.892 kb on + strand at 994.933 kb on - strand, within MMJJ_RS05350 at 994.940 kb on - strand, within MMJJ_RS05350 at 994.944 kb on - strand, within MMJJ_RS05350 at 994.944 kb on - strand, within MMJJ_RS05350 at 994.954 kb on + strand, within MMJJ_RS05350 at 995.031 kb on - strand, within MMJJ_RS05350 at 995.070 kb on - strand, within MMJJ_RS05350 at 995.073 kb on + strand, within MMJJ_RS05350 at 995.073 kb on + strand, within MMJJ_RS05350 at 995.098 kb on + strand, within MMJJ_RS05350 at 995.141 kb on + strand, within MMJJ_RS05350 at 995.163 kb on - strand, within MMJJ_RS05350 at 995.192 kb on - strand, within MMJJ_RS05350 at 995.204 kb on - strand at 995.231 kb on + strand at 995.315 kb on - strand at 995.316 kb on + strand at 995.317 kb on - strand at 995.382 kb on + strand, within MMJJ_RS05355 at 995.382 kb on + strand, within MMJJ_RS05355 at 995.387 kb on + strand, within MMJJ_RS05355 at 995.409 kb on - strand, within MMJJ_RS05355 at 995.410 kb on + strand, within MMJJ_RS05355 at 995.411 kb on - strand, within MMJJ_RS05355 at 995.411 kb on - strand, within MMJJ_RS05355 at 995.411 kb on - strand, within MMJJ_RS05355 at 995.412 kb on + strand, within MMJJ_RS05355 at 995.483 kb on - strand, within MMJJ_RS05355 at 995.523 kb on - strand, within MMJJ_RS05355 at 995.527 kb on - strand, within MMJJ_RS05355 at 995.531 kb on - strand, within MMJJ_RS05355 at 995.592 kb on - strand, within MMJJ_RS05355 at 995.612 kb on - strand, within MMJJ_RS05355 at 995.613 kb on + strand, within MMJJ_RS05355 at 995.613 kb on + strand, within MMJJ_RS05355 at 995.706 kb on + strand, within MMJJ_RS05355 at 995.729 kb on - strand, within MMJJ_RS05355 at 995.729 kb on - strand, within MMJJ_RS05355 at 995.729 kb on - strand, within MMJJ_RS05355 at 995.730 kb on + strand, within MMJJ_RS05355 at 995.731 kb on - strand, within MMJJ_RS05355 at 995.874 kb on + strand, within MMJJ_RS05355 at 995.886 kb on + strand, within MMJJ_RS05355
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE remove 993,152 - MMJJ_RS05340 0.33 -2.4 993,165 - MMJJ_RS05340 0.34 +1.8 993,168 - MMJJ_RS05340 0.35 -0.9 993,177 - MMJJ_RS05340 0.35 +1.3 993,177 - MMJJ_RS05340 0.35 +1.6 993,178 + MMJJ_RS05340 0.36 +1.0 993,178 + MMJJ_RS05340 0.36 -1.4 993,236 - MMJJ_RS05340 0.41 -1.4 993,240 - MMJJ_RS05340 0.41 +0.7 993,240 - MMJJ_RS05340 0.41 +2.5 993,255 + MMJJ_RS05340 0.42 +0.0 993,320 - MMJJ_RS05340 0.48 +1.1 993,339 - MMJJ_RS05340 0.50 -0.6 993,339 - MMJJ_RS05340 0.50 -2.7 993,340 + MMJJ_RS05340 0.50 +2.3 993,340 + MMJJ_RS05340 0.50 -2.5 993,352 + MMJJ_RS05340 0.51 +1.8 993,418 + MMJJ_RS05340 0.57 -1.9 993,418 + MMJJ_RS05340 0.57 +1.0 993,447 - MMJJ_RS05340 0.60 -2.8 993,466 - MMJJ_RS05340 0.61 +1.3 993,466 - MMJJ_RS05340 0.61 -1.5 993,471 - MMJJ_RS05340 0.62 -1.2 993,561 + MMJJ_RS05340 0.70 -1.8 993,579 + MMJJ_RS05340 0.72 +2.0 993,583 + MMJJ_RS05340 0.72 -1.5 993,635 - MMJJ_RS05340 0.77 +2.1 993,636 + MMJJ_RS05340 0.77 -1.8 993,636 + MMJJ_RS05340 0.77 -2.2 993,636 + MMJJ_RS05340 0.77 -0.7 993,670 + MMJJ_RS05340 0.80 +1.1 993,670 + MMJJ_RS05340 0.80 +1.3 993,673 - MMJJ_RS05340 0.80 +1.7 993,673 - MMJJ_RS05340 0.80 -0.6 993,674 + MMJJ_RS05340 0.80 -0.8 993,718 - MMJJ_RS05340 0.84 +2.2 993,725 - MMJJ_RS05340 0.85 -0.9 993,726 + MMJJ_RS05340 0.85 +2.0 993,729 - MMJJ_RS05340 0.85 +1.5 993,744 - MMJJ_RS05340 0.86 +2.1 993,744 - MMJJ_RS05340 0.86 +0.8 993,750 + MMJJ_RS05340 0.87 -0.3 993,809 + +1.3 993,809 + +1.1 993,888 + +0.9 993,913 - +1.7 993,913 - -2.4 993,914 + -1.7 993,914 + -2.2 993,914 + +1.3 993,916 + +2.0 993,922 + +1.4 993,936 + -0.3 993,954 - -0.8 993,958 + +0.3 993,962 + -1.0 993,962 + +0.9 993,971 - -1.1 993,971 - -1.5 993,971 - -2.5 993,971 - -2.8 993,971 - +1.1 993,971 - +1.4 993,971 - -1.2 993,972 + -1.4 993,972 + -1.9 993,972 + +0.9 993,975 - +1.3 993,975 - -1.1 993,975 - -2.2 993,975 - +1.3 993,975 - -1.2 993,975 - +1.5 993,976 + -0.7 993,976 + -2.2 993,976 + +0.9 993,980 - +0.8 993,985 - -1.4 993,989 + +1.8 993,999 - -1.7 993,999 - -1.8 994,000 + -1.1 994,000 + +1.1 994,000 + +2.2 994,001 - -0.9 994,001 - -0.5 994,001 - -5.9 994,001 - +0.7 994,001 - +1.3 994,001 - -0.3 994,002 + -1.9 994,002 + -2.1 994,002 + -1.9 994,002 + -2.4 994,002 + +1.4 994,003 - +1.9 994,003 - -2.6 994,003 - +1.4 994,003 - -2.4 994,003 - -1.7 994,003 - +0.9 994,004 + -1.0 994,004 + -1.7 994,004 + -2.5 994,004 + +1.1 994,004 + +1.0 994,004 + -0.5 994,004 + -0.5 994,004 + -0.4 994,004 + +2.2 994,004 + +2.0 994,004 + +1.7 994,005 - -0.8 994,005 - -1.1 994,005 - +1.0 994,005 - +0.5 994,005 - -1.9 994,005 - -0.9 994,005 - +2.4 994,005 - +0.1 994,005 - -2.4 994,005 - -0.2 994,006 + +0.9 994,006 + -0.9 994,006 + +1.8 994,006 + +2.0 994,006 + -1.5 994,006 + -0.9 994,006 + +1.0 994,006 + +2.0 994,006 + +0.7 994,006 + -2.0 994,006 + -1.9 994,011 + +1.3 994,011 + +0.9 994,015 - +2.4 994,015 - +1.1 994,015 - -3.2 994,015 - +3.1 994,016 + +1.7 994,020 + +1.0 994,020 + +0.7 994,028 - +2.0 994,029 + +1.3 994,029 + -1.0 994,141 - +0.9 994,142 + +0.6 994,145 - +0.1 994,146 + +0.8 994,147 - +1.4 994,147 - +1.2 994,147 - +3.4 994,147 - +2.3 994,147 - +0.6 994,148 + +1.9 994,148 + +1.4 994,148 + +1.3 994,148 + -2.1 994,148 + +0.5 994,156 + +1.2 994,174 + MMJJ_RS05345 0.12 +1.6 994,259 - MMJJ_RS05345 0.22 +0.9 994,261 - MMJJ_RS05345 0.22 -2.8 994,262 + MMJJ_RS05345 0.23 +0.7 994,262 + MMJJ_RS05345 0.23 +0.1 994,265 - MMJJ_RS05345 0.23 +1.2 994,265 - MMJJ_RS05345 0.23 +1.8 994,265 - MMJJ_RS05345 0.23 -3.0 994,265 - MMJJ_RS05345 0.23 +1.0 994,266 + MMJJ_RS05345 0.23 -2.2 994,266 + MMJJ_RS05345 0.23 +3.3 994,266 + MMJJ_RS05345 0.23 +0.7 994,266 + MMJJ_RS05345 0.23 -1.6 994,266 + MMJJ_RS05345 0.23 +0.9 994,266 + MMJJ_RS05345 0.23 +1.5 994,266 + MMJJ_RS05345 0.23 -3.6 994,266 + MMJJ_RS05345 0.23 +1.8 994,266 - MMJJ_RS05345 0.23 +2.9 994,267 - MMJJ_RS05345 0.23 +1.3 994,268 + MMJJ_RS05345 0.23 +1.2 994,270 - MMJJ_RS05345 0.24 +1.4 994,275 - MMJJ_RS05345 0.24 -2.3 994,306 - MMJJ_RS05345 0.28 +0.7 994,306 - MMJJ_RS05345 0.28 +1.1 994,339 + MMJJ_RS05345 0.32 +2.0 994,339 + MMJJ_RS05345 0.32 -3.2 994,339 + MMJJ_RS05345 0.32 +1.1 994,342 + MMJJ_RS05345 0.32 +1.3 994,366 + MMJJ_RS05345 0.35 -1.1 994,366 + MMJJ_RS05345 0.35 +1.7 994,424 + MMJJ_RS05345 0.42 +0.7 994,424 + MMJJ_RS05345 0.42 -2.5 994,461 - MMJJ_RS05345 0.47 +0.8 994,486 + MMJJ_RS05345 0.50 -2.2 994,632 - MMJJ_RS05345 0.67 +0.7 994,655 - MMJJ_RS05345 0.70 +0.1 994,656 + MMJJ_RS05345 0.70 -2.4 994,660 - MMJJ_RS05345 0.71 +0.8 994,660 - MMJJ_RS05345 0.71 -3.6 994,661 + MMJJ_RS05345 0.71 -2.5 994,786 - MMJJ_RS05345 0.86 +1.0 994,862 - -1.4 994,863 + +0.9 994,892 + +1.3 994,933 - MMJJ_RS05350 0.11 -2.3 994,940 - MMJJ_RS05350 0.13 +1.7 994,944 - MMJJ_RS05350 0.14 -2.0 994,944 - MMJJ_RS05350 0.14 -1.0 994,954 + MMJJ_RS05350 0.17 +0.7 995,031 - MMJJ_RS05350 0.40 +2.0 995,070 - MMJJ_RS05350 0.51 +1.6 995,073 + MMJJ_RS05350 0.52 +1.2 995,073 + MMJJ_RS05350 0.52 -2.3 995,098 + MMJJ_RS05350 0.60 +1.8 995,141 + MMJJ_RS05350 0.73 -0.9 995,163 - MMJJ_RS05350 0.79 -0.9 995,192 - MMJJ_RS05350 0.88 -3.3 995,204 - +1.3 995,231 + +1.4 995,315 - -1.2 995,316 + -0.8 995,317 - -2.4 995,382 + MMJJ_RS05355 0.17 -2.0 995,382 + MMJJ_RS05355 0.17 -3.1 995,387 + MMJJ_RS05355 0.17 +0.4 995,409 - MMJJ_RS05355 0.20 +1.6 995,410 + MMJJ_RS05355 0.20 +0.7 995,411 - MMJJ_RS05355 0.20 -1.0 995,411 - MMJJ_RS05355 0.20 +2.0 995,411 - MMJJ_RS05355 0.20 -1.5 995,412 + MMJJ_RS05355 0.20 +1.5 995,483 - MMJJ_RS05355 0.28 -1.9 995,523 - MMJJ_RS05355 0.33 +1.1 995,527 - MMJJ_RS05355 0.33 -1.4 995,531 - MMJJ_RS05355 0.34 -0.6 995,592 - MMJJ_RS05355 0.41 +0.8 995,612 - MMJJ_RS05355 0.43 +1.7 995,613 + MMJJ_RS05355 0.43 +1.6 995,613 + MMJJ_RS05355 0.43 +0.0 995,706 + MMJJ_RS05355 0.54 -3.0 995,729 - MMJJ_RS05355 0.56 -1.5 995,729 - MMJJ_RS05355 0.56 -0.5 995,729 - MMJJ_RS05355 0.56 -1.9 995,730 + MMJJ_RS05355 0.56 -0.1 995,731 - MMJJ_RS05355 0.56 +2.2 995,874 + MMJJ_RS05355 0.73 +1.1 995,886 + MMJJ_RS05355 0.74 -0.0
Or see this region's nucleotide sequence