Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS01445 and MMJJ_RS01450 are separated by 79 nucleotides MMJJ_RS01450 and MMJJ_RS01455 are separated by 164 nucleotides MMJJ_RS01455 and MMJJ_RS01460 are separated by 78 nucleotides
MMJJ_RS01445: MMJJ_RS01445 - O-phosphoserine--tRNA ligase, at 281,949 to 283,562
_RS01445
MMJJ_RS01450: MMJJ_RS01450 - iron ABC transporter substrate-binding protein, at 283,642 to 284,769
_RS01450
MMJJ_RS01455: MMJJ_RS01455 - triose-phosphate isomerase, at 284,934 to 285,599
_RS01455
MMJJ_RS01460: MMJJ_RS01460 - 2-amino-3,7-dideoxy-D-threo-hept-6-ulosonate synthase, at 285,678 to 286,496
_RS01460
Position (kb) 283
284
285 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 282.677 kb on - strand, within MMJJ_RS01445 at 282.887 kb on + strand, within MMJJ_RS01445 at 283.590 kb on - strand at 283.591 kb on + strand at 283.601 kb on - strand at 283.601 kb on - strand at 283.627 kb on - strand at 283.643 kb on - strand at 283.643 kb on - strand at 283.644 kb on + strand at 283.660 kb on + strand at 283.709 kb on - strand at 283.734 kb on - strand at 283.735 kb on + strand at 283.736 kb on - strand at 283.736 kb on - strand at 283.736 kb on - strand at 283.737 kb on + strand at 283.737 kb on + strand at 283.738 kb on - strand at 283.738 kb on - strand at 283.738 kb on - strand at 283.738 kb on - strand at 283.738 kb on - strand at 283.738 kb on - strand at 283.738 kb on - strand at 283.739 kb on + strand at 283.739 kb on + strand at 283.739 kb on + strand at 283.739 kb on + strand at 283.739 kb on + strand at 283.739 kb on + strand at 283.739 kb on + strand at 283.739 kb on + strand at 283.739 kb on + strand at 283.739 kb on + strand at 283.740 kb on - strand at 283.741 kb on + strand at 283.765 kb on - strand, within MMJJ_RS01450 at 283.787 kb on - strand, within MMJJ_RS01450 at 283.789 kb on - strand, within MMJJ_RS01450 at 283.804 kb on + strand, within MMJJ_RS01450 at 283.804 kb on + strand, within MMJJ_RS01450 at 283.806 kb on + strand, within MMJJ_RS01450 at 283.806 kb on + strand, within MMJJ_RS01450 at 283.806 kb on + strand, within MMJJ_RS01450 at 283.806 kb on + strand, within MMJJ_RS01450 at 283.834 kb on + strand, within MMJJ_RS01450 at 283.834 kb on + strand, within MMJJ_RS01450 at 283.838 kb on + strand, within MMJJ_RS01450 at 283.842 kb on - strand, within MMJJ_RS01450 at 283.844 kb on - strand, within MMJJ_RS01450 at 283.845 kb on + strand, within MMJJ_RS01450 at 283.852 kb on + strand, within MMJJ_RS01450 at 283.852 kb on + strand, within MMJJ_RS01450 at 283.873 kb on + strand, within MMJJ_RS01450 at 283.893 kb on - strand, within MMJJ_RS01450 at 283.894 kb on + strand, within MMJJ_RS01450 at 283.902 kb on - strand, within MMJJ_RS01450 at 283.926 kb on + strand, within MMJJ_RS01450 at 283.936 kb on - strand, within MMJJ_RS01450 at 283.937 kb on + strand, within MMJJ_RS01450 at 283.960 kb on - strand, within MMJJ_RS01450 at 284.221 kb on - strand, within MMJJ_RS01450 at 284.222 kb on + strand at 284.222 kb on + strand, within MMJJ_RS01450 at 284.222 kb on + strand, within MMJJ_RS01450 at 284.236 kb on + strand, within MMJJ_RS01450 at 284.279 kb on - strand, within MMJJ_RS01450 at 284.282 kb on + strand, within MMJJ_RS01450 at 284.321 kb on - strand, within MMJJ_RS01450 at 284.387 kb on - strand, within MMJJ_RS01450 at 284.387 kb on - strand, within MMJJ_RS01450 at 284.388 kb on + strand, within MMJJ_RS01450 at 284.388 kb on + strand, within MMJJ_RS01450 at 284.388 kb on + strand, within MMJJ_RS01450 at 284.391 kb on - strand, within MMJJ_RS01450 at 284.401 kb on - strand, within MMJJ_RS01450 at 284.401 kb on - strand, within MMJJ_RS01450 at 284.402 kb on + strand, within MMJJ_RS01450 at 284.413 kb on + strand, within MMJJ_RS01450 at 284.464 kb on - strand, within MMJJ_RS01450 at 284.465 kb on + strand, within MMJJ_RS01450 at 284.467 kb on - strand, within MMJJ_RS01450 at 284.467 kb on - strand, within MMJJ_RS01450 at 284.505 kb on - strand, within MMJJ_RS01450 at 284.506 kb on + strand, within MMJJ_RS01450 at 284.506 kb on + strand, within MMJJ_RS01450 at 284.508 kb on + strand, within MMJJ_RS01450 at 284.556 kb on - strand, within MMJJ_RS01450 at 284.556 kb on - strand, within MMJJ_RS01450 at 284.556 kb on - strand, within MMJJ_RS01450 at 284.557 kb on + strand, within MMJJ_RS01450 at 284.569 kb on - strand, within MMJJ_RS01450 at 284.569 kb on - strand, within MMJJ_RS01450 at 284.570 kb on + strand, within MMJJ_RS01450 at 284.570 kb on + strand, within MMJJ_RS01450 at 284.570 kb on + strand, within MMJJ_RS01450 at 284.573 kb on - strand, within MMJJ_RS01450 at 284.608 kb on - strand, within MMJJ_RS01450 at 284.608 kb on - strand, within MMJJ_RS01450 at 284.609 kb on + strand, within MMJJ_RS01450 at 284.609 kb on + strand, within MMJJ_RS01450 at 284.609 kb on + strand, within MMJJ_RS01450 at 284.609 kb on + strand, within MMJJ_RS01450 at 284.609 kb on + strand, within MMJJ_RS01450 at 284.609 kb on + strand, within MMJJ_RS01450 at 284.609 kb on + strand, within MMJJ_RS01450 at 284.617 kb on + strand, within MMJJ_RS01450 at 284.655 kb on - strand, within MMJJ_RS01450 at 284.664 kb on - strand at 284.677 kb on + strand at 284.697 kb on - strand at 284.698 kb on + strand at 284.709 kb on - strand at 284.769 kb on - strand at 284.770 kb on + strand at 284.796 kb on - strand at 284.824 kb on + strand at 284.837 kb on - strand at 284.842 kb on + strand at 284.878 kb on - strand at 284.929 kb on - strand at 285.481 kb on + strand, within MMJJ_RS01455 at 285.574 kb on - strand at 285.586 kb on + strand at 285.599 kb on - strand at 285.619 kb on + strand at 285.620 kb on - strand at 285.624 kb on + strand at 285.644 kb on - strand at 285.649 kb on + strand at 285.649 kb on + strand at 285.649 kb on + strand at 285.696 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE remove 282,677 - MMJJ_RS01445 0.45 -2.1 282,887 + MMJJ_RS01445 0.58 -1.1 283,590 - -0.1 283,591 + -2.4 283,601 - -1.9 283,601 - -1.7 283,627 - +0.5 283,643 - +1.2 283,643 - -0.1 283,644 + -1.1 283,660 + +1.4 283,709 - +1.7 283,734 - +1.0 283,735 + +2.2 283,736 - +1.2 283,736 - -0.7 283,736 - -1.9 283,737 + -2.1 283,737 + -1.8 283,738 - +0.9 283,738 - -1.9 283,738 - +1.0 283,738 - -2.1 283,738 - +1.5 283,738 - +0.7 283,738 - -1.4 283,739 + -1.9 283,739 + +1.2 283,739 + -2.4 283,739 + -1.4 283,739 + +1.7 283,739 + +1.7 283,739 + -1.4 283,739 + +1.6 283,739 + +0.8 283,739 + +1.6 283,740 - +0.6 283,741 + -2.6 283,765 - MMJJ_RS01450 0.11 +1.2 283,787 - MMJJ_RS01450 0.13 +1.1 283,789 - MMJJ_RS01450 0.13 -2.1 283,804 + MMJJ_RS01450 0.14 +1.1 283,804 + MMJJ_RS01450 0.14 -1.8 283,806 + MMJJ_RS01450 0.15 +0.9 283,806 + MMJJ_RS01450 0.15 +1.0 283,806 + MMJJ_RS01450 0.15 +0.6 283,806 + MMJJ_RS01450 0.15 +0.1 283,834 + MMJJ_RS01450 0.17 -0.9 283,834 + MMJJ_RS01450 0.17 -1.2 283,838 + MMJJ_RS01450 0.17 -1.5 283,842 - MMJJ_RS01450 0.18 -2.0 283,844 - MMJJ_RS01450 0.18 -1.7 283,845 + MMJJ_RS01450 0.18 +0.6 283,852 + MMJJ_RS01450 0.19 +1.6 283,852 + MMJJ_RS01450 0.19 -1.7 283,873 + MMJJ_RS01450 0.20 +0.6 283,893 - MMJJ_RS01450 0.22 -0.1 283,894 + MMJJ_RS01450 0.22 -1.0 283,902 - MMJJ_RS01450 0.23 +1.2 283,926 + MMJJ_RS01450 0.25 -1.8 283,936 - MMJJ_RS01450 0.26 +1.1 283,937 + MMJJ_RS01450 0.26 -2.0 283,960 - MMJJ_RS01450 0.28 -1.8 284,221 - MMJJ_RS01450 0.51 +1.1 284,222 + -1.1 284,222 + MMJJ_RS01450 0.51 -2.7 284,222 + MMJJ_RS01450 0.51 -1.8 284,236 + MMJJ_RS01450 0.53 +1.0 284,279 - MMJJ_RS01450 0.56 -3.7 284,282 + MMJJ_RS01450 0.57 +1.0 284,321 - MMJJ_RS01450 0.60 -2.4 284,387 - MMJJ_RS01450 0.66 -1.9 284,387 - MMJJ_RS01450 0.66 -2.8 284,388 + MMJJ_RS01450 0.66 -1.7 284,388 + MMJJ_RS01450 0.66 -1.1 284,388 + MMJJ_RS01450 0.66 +1.5 284,391 - MMJJ_RS01450 0.66 -2.2 284,401 - MMJJ_RS01450 0.67 -2.7 284,401 - MMJJ_RS01450 0.67 -0.1 284,402 + MMJJ_RS01450 0.67 -0.1 284,413 + MMJJ_RS01450 0.68 -2.4 284,464 - MMJJ_RS01450 0.73 -0.2 284,465 + MMJJ_RS01450 0.73 +0.8 284,467 - MMJJ_RS01450 0.73 +0.5 284,467 - MMJJ_RS01450 0.73 +1.3 284,505 - MMJJ_RS01450 0.77 -2.6 284,506 + MMJJ_RS01450 0.77 -2.5 284,506 + MMJJ_RS01450 0.77 +1.5 284,508 + MMJJ_RS01450 0.77 +1.1 284,556 - MMJJ_RS01450 0.81 +1.1 284,556 - MMJJ_RS01450 0.81 +0.7 284,556 - MMJJ_RS01450 0.81 +0.5 284,557 + MMJJ_RS01450 0.81 -1.5 284,569 - MMJJ_RS01450 0.82 +0.8 284,569 - MMJJ_RS01450 0.82 -2.6 284,570 + MMJJ_RS01450 0.82 -1.8 284,570 + MMJJ_RS01450 0.82 -2.0 284,570 + MMJJ_RS01450 0.82 +2.1 284,573 - MMJJ_RS01450 0.83 -1.4 284,608 - MMJJ_RS01450 0.86 +1.4 284,608 - MMJJ_RS01450 0.86 +0.3 284,609 + MMJJ_RS01450 0.86 +0.7 284,609 + MMJJ_RS01450 0.86 -2.0 284,609 + MMJJ_RS01450 0.86 -2.3 284,609 + MMJJ_RS01450 0.86 +1.6 284,609 + MMJJ_RS01450 0.86 -1.0 284,609 + MMJJ_RS01450 0.86 +0.7 284,609 + MMJJ_RS01450 0.86 +1.2 284,617 + MMJJ_RS01450 0.86 -3.2 284,655 - MMJJ_RS01450 0.90 -3.7 284,664 - -0.5 284,677 + +1.0 284,697 - -1.7 284,698 + -2.7 284,709 - +1.0 284,769 - -0.1 284,770 + +1.1 284,796 - +1.0 284,824 + -1.7 284,837 - +0.4 284,842 + -1.1 284,878 - -2.7 284,929 - -3.5 285,481 + MMJJ_RS01455 0.82 -2.2 285,574 - -2.1 285,586 + -2.1 285,599 - -1.7 285,619 + -0.1 285,620 - -2.8 285,624 + -3.7 285,644 - -2.6 285,649 + +1.1 285,649 + -3.4 285,649 + +0.5 285,696 - +1.8
Or see this region's nucleotide sequence