Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS00940 and MMJJ_RS00945 are separated by 139 nucleotides MMJJ_RS00945 and MMJJ_RS00950 are separated by 104 nucleotides MMJJ_RS00950 and MMJJ_RS00955 are separated by 30 nucleotides MMJJ_RS00955 and MMJJ_RS00960 are separated by 57 nucleotides
MMJJ_RS00940: MMJJ_RS00940 - aldo/keto reductase, at 181,899 to 183,092
_RS00940
MMJJ_RS00945: MMJJ_RS00945 - carboxymuconolactone decarboxylase family protein, at 183,232 to 183,963
_RS00945
MMJJ_RS00950: MMJJ_RS00950 - DUF111 family protein, at 184,068 to 184,787
_RS00950
MMJJ_RS00955: MMJJ_RS00955 - flavodoxin, at 184,818 to 185,353
_RS00955
MMJJ_RS00960: MMJJ_RS00960 - flavodoxin family protein, at 185,411 to 186,046
_RS00960
Position (kb) 184
185 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 183.110 kb on - strand at 183.111 kb on + strand at 183.113 kb on + strand at 183.126 kb on - strand at 183.150 kb on + strand at 183.166 kb on - strand at 183.166 kb on - strand at 183.166 kb on - strand at 183.171 kb on - strand at 183.172 kb on + strand at 183.200 kb on - strand at 183.200 kb on - strand at 183.200 kb on - strand at 183.200 kb on - strand at 183.201 kb on + strand at 183.201 kb on + strand at 183.201 kb on + strand at 183.201 kb on + strand at 183.206 kb on - strand at 183.210 kb on + strand at 183.215 kb on + strand at 183.234 kb on + strand at 183.234 kb on + strand at 183.253 kb on - strand at 183.289 kb on - strand at 183.310 kb on + strand, within MMJJ_RS00945 at 183.322 kb on - strand, within MMJJ_RS00945 at 183.323 kb on + strand, within MMJJ_RS00945 at 183.323 kb on + strand, within MMJJ_RS00945 at 183.325 kb on - strand, within MMJJ_RS00945 at 183.325 kb on - strand, within MMJJ_RS00945 at 183.363 kb on - strand, within MMJJ_RS00945 at 183.364 kb on + strand, within MMJJ_RS00945 at 183.364 kb on + strand, within MMJJ_RS00945 at 183.374 kb on + strand, within MMJJ_RS00945 at 183.407 kb on + strand, within MMJJ_RS00945 at 183.407 kb on + strand, within MMJJ_RS00945 at 183.408 kb on - strand, within MMJJ_RS00945 at 183.408 kb on - strand, within MMJJ_RS00945 at 183.458 kb on - strand, within MMJJ_RS00945 at 183.459 kb on + strand, within MMJJ_RS00945 at 183.459 kb on + strand, within MMJJ_RS00945 at 183.459 kb on + strand, within MMJJ_RS00945 at 183.488 kb on - strand, within MMJJ_RS00945 at 183.488 kb on - strand, within MMJJ_RS00945 at 183.502 kb on - strand, within MMJJ_RS00945 at 183.502 kb on - strand, within MMJJ_RS00945 at 183.502 kb on - strand, within MMJJ_RS00945 at 183.527 kb on - strand, within MMJJ_RS00945 at 183.528 kb on + strand, within MMJJ_RS00945 at 183.589 kb on + strand, within MMJJ_RS00945 at 183.620 kb on + strand, within MMJJ_RS00945 at 183.625 kb on - strand, within MMJJ_RS00945 at 183.631 kb on - strand, within MMJJ_RS00945 at 183.678 kb on + strand, within MMJJ_RS00945 at 183.678 kb on + strand, within MMJJ_RS00945 at 183.698 kb on - strand, within MMJJ_RS00945 at 183.698 kb on - strand, within MMJJ_RS00945 at 183.713 kb on - strand, within MMJJ_RS00945 at 183.713 kb on - strand, within MMJJ_RS00945 at 183.715 kb on - strand, within MMJJ_RS00945 at 183.715 kb on - strand, within MMJJ_RS00945 at 183.715 kb on - strand, within MMJJ_RS00945 at 183.715 kb on - strand, within MMJJ_RS00945 at 183.716 kb on + strand, within MMJJ_RS00945 at 183.716 kb on + strand, within MMJJ_RS00945 at 183.718 kb on - strand, within MMJJ_RS00945 at 183.718 kb on - strand, within MMJJ_RS00945 at 183.719 kb on + strand, within MMJJ_RS00945 at 183.719 kb on + strand, within MMJJ_RS00945 at 183.735 kb on - strand, within MMJJ_RS00945 at 183.735 kb on - strand, within MMJJ_RS00945 at 183.742 kb on - strand, within MMJJ_RS00945 at 183.773 kb on + strand, within MMJJ_RS00945 at 183.777 kb on - strand, within MMJJ_RS00945 at 183.778 kb on + strand, within MMJJ_RS00945 at 183.861 kb on - strand, within MMJJ_RS00945 at 183.862 kb on + strand, within MMJJ_RS00945 at 183.914 kb on + strand at 183.918 kb on - strand at 183.918 kb on - strand at 183.964 kb on + strand at 184.025 kb on - strand at 184.028 kb on - strand at 184.028 kb on - strand at 184.029 kb on + strand at 184.029 kb on + strand at 184.031 kb on + strand at 184.037 kb on - strand at 184.046 kb on + strand at 184.046 kb on + strand at 184.046 kb on + strand at 184.053 kb on + strand at 184.099 kb on + strand at 184.148 kb on - strand, within MMJJ_RS00950 at 184.211 kb on - strand, within MMJJ_RS00950 at 184.358 kb on + strand, within MMJJ_RS00950 at 184.554 kb on - strand, within MMJJ_RS00950 at 184.555 kb on + strand, within MMJJ_RS00950 at 184.556 kb on - strand, within MMJJ_RS00950 at 184.556 kb on - strand, within MMJJ_RS00950 at 184.557 kb on + strand, within MMJJ_RS00950 at 184.557 kb on + strand, within MMJJ_RS00950 at 184.563 kb on - strand, within MMJJ_RS00950 at 184.577 kb on + strand, within MMJJ_RS00950 at 184.743 kb on + strand at 184.775 kb on - strand at 184.830 kb on - strand at 184.935 kb on + strand, within MMJJ_RS00955 at 184.973 kb on - strand, within MMJJ_RS00955 at 184.985 kb on - strand, within MMJJ_RS00955 at 185.008 kb on - strand, within MMJJ_RS00955 at 185.024 kb on - strand, within MMJJ_RS00955 at 185.024 kb on - strand, within MMJJ_RS00955 at 185.068 kb on + strand, within MMJJ_RS00955 at 185.073 kb on - strand, within MMJJ_RS00955 at 185.074 kb on + strand, within MMJJ_RS00955 at 185.074 kb on + strand, within MMJJ_RS00955 at 185.074 kb on + strand, within MMJJ_RS00955 at 185.093 kb on - strand, within MMJJ_RS00955 at 185.096 kb on - strand, within MMJJ_RS00955 at 185.097 kb on + strand, within MMJJ_RS00955 at 185.112 kb on - strand, within MMJJ_RS00955 at 185.159 kb on + strand, within MMJJ_RS00955 at 185.182 kb on + strand, within MMJJ_RS00955 at 185.189 kb on - strand, within MMJJ_RS00955 at 185.190 kb on + strand, within MMJJ_RS00955 at 185.190 kb on + strand, within MMJJ_RS00955 at 185.190 kb on + strand, within MMJJ_RS00955 at 185.190 kb on + strand, within MMJJ_RS00955 at 185.261 kb on - strand, within MMJJ_RS00955 at 185.261 kb on - strand, within MMJJ_RS00955 at 185.292 kb on - strand, within MMJJ_RS00955 at 185.293 kb on + strand, within MMJJ_RS00955 at 185.293 kb on + strand, within MMJJ_RS00955 at 185.293 kb on + strand, within MMJJ_RS00955 at 185.293 kb on + strand, within MMJJ_RS00955 at 185.293 kb on + strand, within MMJJ_RS00955 at 185.296 kb on - strand, within MMJJ_RS00955 at 185.297 kb on + strand, within MMJJ_RS00955 at 185.300 kb on + strand at 185.357 kb on + strand at 185.462 kb on - strand at 185.488 kb on - strand, within MMJJ_RS00960 at 185.509 kb on - strand, within MMJJ_RS00960 at 185.509 kb on - strand, within MMJJ_RS00960 at 185.558 kb on - strand, within MMJJ_RS00960 at 185.596 kb on + strand, within MMJJ_RS00960 at 185.599 kb on - strand, within MMJJ_RS00960 at 185.600 kb on + strand, within MMJJ_RS00960 at 185.600 kb on + strand, within MMJJ_RS00960 at 185.639 kb on + strand, within MMJJ_RS00960 at 185.730 kb on + strand, within MMJJ_RS00960 at 185.740 kb on - strand, within MMJJ_RS00960 at 185.742 kb on - strand, within MMJJ_RS00960 at 185.743 kb on + strand, within MMJJ_RS00960 at 185.770 kb on - strand, within MMJJ_RS00960
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE remove 183,110 - +0.4 183,111 + -3.6 183,113 + -2.1 183,126 - +1.2 183,150 + +0.7 183,166 - +1.2 183,166 - -2.2 183,166 - +2.3 183,171 - +1.1 183,172 + -1.9 183,200 - -3.1 183,200 - +2.1 183,200 - +0.4 183,200 - -0.7 183,201 + -0.1 183,201 + -1.9 183,201 + +1.1 183,201 + -1.0 183,206 - -1.6 183,210 + +0.8 183,215 + -1.5 183,234 + -1.4 183,234 + -1.5 183,253 - +1.2 183,289 - -1.8 183,310 + MMJJ_RS00945 0.11 +0.8 183,322 - MMJJ_RS00945 0.12 -2.3 183,323 + MMJJ_RS00945 0.12 +0.3 183,323 + MMJJ_RS00945 0.12 +0.9 183,325 - MMJJ_RS00945 0.13 -2.4 183,325 - MMJJ_RS00945 0.13 -3.1 183,363 - MMJJ_RS00945 0.18 +0.4 183,364 + MMJJ_RS00945 0.18 +1.3 183,364 + MMJJ_RS00945 0.18 +0.9 183,374 + MMJJ_RS00945 0.19 -1.2 183,407 + MMJJ_RS00945 0.24 +1.1 183,407 + MMJJ_RS00945 0.24 +2.3 183,408 - MMJJ_RS00945 0.24 +1.1 183,408 - MMJJ_RS00945 0.24 -0.9 183,458 - MMJJ_RS00945 0.31 -1.1 183,459 + MMJJ_RS00945 0.31 +0.1 183,459 + MMJJ_RS00945 0.31 -1.7 183,459 + MMJJ_RS00945 0.31 +0.4 183,488 - MMJJ_RS00945 0.35 -1.4 183,488 - MMJJ_RS00945 0.35 +1.2 183,502 - MMJJ_RS00945 0.37 -0.7 183,502 - MMJJ_RS00945 0.37 -2.1 183,502 - MMJJ_RS00945 0.37 +1.5 183,527 - MMJJ_RS00945 0.40 -1.1 183,528 + MMJJ_RS00945 0.40 +1.3 183,589 + MMJJ_RS00945 0.49 +1.3 183,620 + MMJJ_RS00945 0.53 +0.8 183,625 - MMJJ_RS00945 0.54 +2.0 183,631 - MMJJ_RS00945 0.55 -2.7 183,678 + MMJJ_RS00945 0.61 -2.1 183,678 + MMJJ_RS00945 0.61 -1.8 183,698 - MMJJ_RS00945 0.64 -2.9 183,698 - MMJJ_RS00945 0.64 -3.0 183,713 - MMJJ_RS00945 0.66 -0.7 183,713 - MMJJ_RS00945 0.66 +1.5 183,715 - MMJJ_RS00945 0.66 +0.7 183,715 - MMJJ_RS00945 0.66 -1.1 183,715 - MMJJ_RS00945 0.66 -1.2 183,715 - MMJJ_RS00945 0.66 +1.6 183,716 + MMJJ_RS00945 0.66 +1.5 183,716 + MMJJ_RS00945 0.66 +1.0 183,718 - MMJJ_RS00945 0.66 -1.1 183,718 - MMJJ_RS00945 0.66 +2.0 183,719 + MMJJ_RS00945 0.67 -3.1 183,719 + MMJJ_RS00945 0.67 +0.4 183,735 - MMJJ_RS00945 0.69 +0.5 183,735 - MMJJ_RS00945 0.69 -0.2 183,742 - MMJJ_RS00945 0.70 +0.9 183,773 + MMJJ_RS00945 0.74 +1.0 183,777 - MMJJ_RS00945 0.74 +2.0 183,778 + MMJJ_RS00945 0.75 +1.1 183,861 - MMJJ_RS00945 0.86 -2.1 183,862 + MMJJ_RS00945 0.86 +1.5 183,914 + +0.4 183,918 - +1.1 183,918 - +1.3 183,964 + -0.4 184,025 - -2.3 184,028 - +0.5 184,028 - +0.9 184,029 + -2.5 184,029 + -4.1 184,031 + -2.4 184,037 - +0.5 184,046 + +1.1 184,046 + +0.9 184,046 + -1.1 184,053 + +1.0 184,099 + +1.3 184,148 - MMJJ_RS00950 0.11 +1.2 184,211 - MMJJ_RS00950 0.20 +1.5 184,358 + MMJJ_RS00950 0.40 -2.0 184,554 - MMJJ_RS00950 0.68 -2.0 184,555 + MMJJ_RS00950 0.68 -2.7 184,556 - MMJJ_RS00950 0.68 +0.4 184,556 - MMJJ_RS00950 0.68 -1.4 184,557 + MMJJ_RS00950 0.68 +1.6 184,557 + MMJJ_RS00950 0.68 -0.8 184,563 - MMJJ_RS00950 0.69 -1.8 184,577 + MMJJ_RS00950 0.71 -4.0 184,743 + +1.2 184,775 - -2.4 184,830 - -2.9 184,935 + MMJJ_RS00955 0.22 -0.7 184,973 - MMJJ_RS00955 0.29 -2.1 184,985 - MMJJ_RS00955 0.31 -3.1 185,008 - MMJJ_RS00955 0.35 -3.7 185,024 - MMJJ_RS00955 0.38 -2.7 185,024 - MMJJ_RS00955 0.38 -2.7 185,068 + MMJJ_RS00955 0.47 -1.9 185,073 - MMJJ_RS00955 0.48 +0.4 185,074 + MMJJ_RS00955 0.48 +1.1 185,074 + MMJJ_RS00955 0.48 -2.0 185,074 + MMJJ_RS00955 0.48 +1.1 185,093 - MMJJ_RS00955 0.51 +0.4 185,096 - MMJJ_RS00955 0.52 +0.4 185,097 + MMJJ_RS00955 0.52 -1.7 185,112 - MMJJ_RS00955 0.55 +0.5 185,159 + MMJJ_RS00955 0.64 +1.6 185,182 + MMJJ_RS00955 0.68 +0.3 185,189 - MMJJ_RS00955 0.69 -1.0 185,190 + MMJJ_RS00955 0.69 +1.3 185,190 + MMJJ_RS00955 0.69 +0.5 185,190 + MMJJ_RS00955 0.69 +0.8 185,190 + MMJJ_RS00955 0.69 -1.9 185,261 - MMJJ_RS00955 0.83 -1.1 185,261 - MMJJ_RS00955 0.83 +1.1 185,292 - MMJJ_RS00955 0.88 +1.0 185,293 + MMJJ_RS00955 0.89 +1.1 185,293 + MMJJ_RS00955 0.89 +0.8 185,293 + MMJJ_RS00955 0.89 -0.1 185,293 + MMJJ_RS00955 0.89 +0.8 185,293 + MMJJ_RS00955 0.89 -2.4 185,296 - MMJJ_RS00955 0.89 +1.1 185,297 + MMJJ_RS00955 0.89 -1.0 185,300 + -0.1 185,357 + +0.7 185,462 - -1.3 185,488 - MMJJ_RS00960 0.12 -1.7 185,509 - MMJJ_RS00960 0.15 +1.2 185,509 - MMJJ_RS00960 0.15 -1.2 185,558 - MMJJ_RS00960 0.23 +0.9 185,596 + MMJJ_RS00960 0.29 -1.7 185,599 - MMJJ_RS00960 0.30 +0.1 185,600 + MMJJ_RS00960 0.30 +0.4 185,600 + MMJJ_RS00960 0.30 +1.0 185,639 + MMJJ_RS00960 0.36 -1.3 185,730 + MMJJ_RS00960 0.50 -2.1 185,740 - MMJJ_RS00960 0.52 +0.9 185,742 - MMJJ_RS00960 0.52 +0.2 185,743 + MMJJ_RS00960 0.52 -0.9 185,770 - MMJJ_RS00960 0.56 -0.4
Or see this region's nucleotide sequence