Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS00395 and MMJJ_RS00400 are separated by 42 nucleotides MMJJ_RS00400 and MMJJ_RS00405 are separated by 76 nucleotides MMJJ_RS00405 and MMJJ_RS00410 are separated by 43 nucleotides MMJJ_RS00410 and MMJJ_RS00415 are separated by 43 nucleotides MMJJ_RS00415 and MMJJ_RS00420 are separated by 14 nucleotides
MMJJ_RS00395: MMJJ_RS00395 - DUF473 domain-containing protein, at 74,673 to 75,050
_RS00395
MMJJ_RS00400: MMJJ_RS00400 - tautomerase family protein, at 75,093 to 75,287
_RS00400
MMJJ_RS00405: MMJJ_RS00405 - flavodoxin family protein, at 75,364 to 75,945
_RS00405
MMJJ_RS00410: MMJJ_RS00410 - hypothetical protein, at 75,989 to 76,417
_RS00410
MMJJ_RS00415: MMJJ_RS00415 - DedA family protein, at 76,461 to 76,943
_RS00415
MMJJ_RS00420: MMJJ_RS00420 - CBS domain-containing protein, at 76,958 to 77,338
_RS00420
Position (kb) 75
76
77 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 75.016 kb on - strand at 75.038 kb on + strand at 75.039 kb on - strand at 75.109 kb on - strand at 75.124 kb on - strand, within MMJJ_RS00400 at 75.181 kb on - strand, within MMJJ_RS00400 at 75.211 kb on - strand, within MMJJ_RS00400 at 75.212 kb on + strand, within MMJJ_RS00400 at 75.215 kb on - strand, within MMJJ_RS00400 at 75.215 kb on - strand, within MMJJ_RS00400 at 75.266 kb on + strand, within MMJJ_RS00400 at 75.266 kb on + strand, within MMJJ_RS00400 at 75.272 kb on - strand at 75.300 kb on - strand at 75.301 kb on + strand at 75.342 kb on - strand at 75.343 kb on + strand at 75.371 kb on - strand at 75.371 kb on - strand at 75.434 kb on - strand, within MMJJ_RS00405 at 75.472 kb on + strand, within MMJJ_RS00405 at 75.494 kb on - strand, within MMJJ_RS00405 at 75.540 kb on - strand, within MMJJ_RS00405 at 75.540 kb on - strand, within MMJJ_RS00405 at 75.541 kb on + strand, within MMJJ_RS00405 at 75.541 kb on + strand, within MMJJ_RS00405 at 75.541 kb on + strand, within MMJJ_RS00405 at 75.548 kb on - strand, within MMJJ_RS00405 at 75.596 kb on - strand, within MMJJ_RS00405 at 75.596 kb on - strand, within MMJJ_RS00405 at 75.616 kb on - strand, within MMJJ_RS00405 at 75.616 kb on - strand, within MMJJ_RS00405 at 75.685 kb on + strand, within MMJJ_RS00405 at 75.754 kb on + strand, within MMJJ_RS00405 at 75.781 kb on + strand, within MMJJ_RS00405 at 75.827 kb on + strand, within MMJJ_RS00405 at 75.941 kb on - strand at 75.942 kb on + strand at 75.943 kb on - strand at 75.946 kb on - strand at 75.947 kb on + strand at 75.966 kb on - strand at 75.976 kb on - strand at 76.026 kb on - strand at 76.030 kb on + strand at 76.077 kb on - strand, within MMJJ_RS00410 at 76.082 kb on + strand, within MMJJ_RS00410 at 76.085 kb on + strand, within MMJJ_RS00410 at 76.086 kb on - strand, within MMJJ_RS00410 at 76.086 kb on - strand, within MMJJ_RS00410 at 76.086 kb on - strand, within MMJJ_RS00410 at 76.086 kb on - strand, within MMJJ_RS00410 at 76.102 kb on - strand, within MMJJ_RS00410 at 76.152 kb on - strand, within MMJJ_RS00410 at 76.198 kb on - strand, within MMJJ_RS00410 at 76.198 kb on - strand, within MMJJ_RS00410 at 76.251 kb on - strand, within MMJJ_RS00410 at 76.252 kb on + strand, within MMJJ_RS00410 at 76.268 kb on - strand, within MMJJ_RS00410 at 76.268 kb on - strand, within MMJJ_RS00410 at 76.269 kb on + strand, within MMJJ_RS00410 at 76.269 kb on + strand, within MMJJ_RS00410 at 76.269 kb on + strand, within MMJJ_RS00410 at 76.269 kb on + strand, within MMJJ_RS00410 at 76.292 kb on + strand, within MMJJ_RS00410 at 76.292 kb on + strand, within MMJJ_RS00410 at 76.292 kb on + strand, within MMJJ_RS00410 at 76.299 kb on + strand, within MMJJ_RS00410 at 76.299 kb on + strand, within MMJJ_RS00410 at 76.416 kb on + strand at 76.416 kb on + strand at 76.416 kb on + strand at 76.424 kb on + strand at 76.458 kb on + strand at 76.458 kb on + strand at 76.463 kb on + strand at 76.463 kb on + strand at 76.482 kb on - strand at 76.483 kb on + strand at 76.513 kb on + strand, within MMJJ_RS00415 at 76.519 kb on + strand, within MMJJ_RS00415 at 76.582 kb on + strand, within MMJJ_RS00415 at 76.647 kb on - strand, within MMJJ_RS00415 at 76.647 kb on - strand, within MMJJ_RS00415 at 76.680 kb on + strand, within MMJJ_RS00415 at 76.751 kb on - strand, within MMJJ_RS00415 at 76.751 kb on - strand, within MMJJ_RS00415 at 76.763 kb on - strand, within MMJJ_RS00415 at 76.783 kb on + strand, within MMJJ_RS00415 at 76.833 kb on + strand, within MMJJ_RS00415 at 76.833 kb on + strand, within MMJJ_RS00415 at 76.836 kb on - strand, within MMJJ_RS00415 at 76.837 kb on + strand, within MMJJ_RS00415 at 76.837 kb on + strand, within MMJJ_RS00415 at 76.838 kb on - strand, within MMJJ_RS00415 at 76.839 kb on + strand, within MMJJ_RS00415 at 76.839 kb on + strand, within MMJJ_RS00415 at 76.839 kb on + strand, within MMJJ_RS00415 at 76.845 kb on - strand, within MMJJ_RS00415 at 76.903 kb on - strand at 76.908 kb on + strand at 76.908 kb on + strand at 76.911 kb on - strand at 76.982 kb on - strand at 76.998 kb on + strand, within MMJJ_RS00420 at 76.999 kb on - strand, within MMJJ_RS00420 at 77.030 kb on - strand, within MMJJ_RS00420 at 77.045 kb on - strand, within MMJJ_RS00420 at 77.108 kb on - strand, within MMJJ_RS00420 at 77.138 kb on - strand, within MMJJ_RS00420 at 77.138 kb on - strand, within MMJJ_RS00420 at 77.138 kb on - strand, within MMJJ_RS00420 at 77.139 kb on + strand, within MMJJ_RS00420 at 77.139 kb on + strand, within MMJJ_RS00420 at 77.199 kb on + strand, within MMJJ_RS00420 at 77.199 kb on + strand, within MMJJ_RS00420 at 77.199 kb on + strand, within MMJJ_RS00420 at 77.200 kb on - strand, within MMJJ_RS00420 at 77.222 kb on - strand, within MMJJ_RS00420 at 77.222 kb on - strand, within MMJJ_RS00420 at 77.223 kb on + strand, within MMJJ_RS00420 at 77.228 kb on - strand, within MMJJ_RS00420 at 77.229 kb on + strand, within MMJJ_RS00420 at 77.252 kb on + strand, within MMJJ_RS00420 at 77.262 kb on + strand, within MMJJ_RS00420 at 77.266 kb on - strand, within MMJJ_RS00420 at 77.367 kb on - strand at 77.370 kb on - strand at 77.370 kb on - strand at 77.371 kb on + strand at 77.372 kb on - strand at 77.372 kb on - strand at 77.373 kb on + strand at 77.373 kb on + strand at 77.374 kb on - strand at 77.374 kb on - strand at 77.375 kb on + strand at 77.379 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE remove 75,016 - +1.2 75,038 + -1.3 75,039 - -0.6 75,109 - +1.1 75,124 - MMJJ_RS00400 0.16 -1.4 75,181 - MMJJ_RS00400 0.45 -1.4 75,211 - MMJJ_RS00400 0.61 +0.9 75,212 + MMJJ_RS00400 0.61 -2.8 75,215 - MMJJ_RS00400 0.63 +1.7 75,215 - MMJJ_RS00400 0.63 +2.0 75,266 + MMJJ_RS00400 0.89 -2.4 75,266 + MMJJ_RS00400 0.89 +0.4 75,272 - -0.8 75,300 - +1.4 75,301 + -0.1 75,342 - +1.0 75,343 + +1.7 75,371 - +0.8 75,371 - +2.0 75,434 - MMJJ_RS00405 0.12 +1.2 75,472 + MMJJ_RS00405 0.19 +1.1 75,494 - MMJJ_RS00405 0.22 +0.4 75,540 - MMJJ_RS00405 0.30 -1.1 75,540 - MMJJ_RS00405 0.30 +1.5 75,541 + MMJJ_RS00405 0.30 +0.3 75,541 + MMJJ_RS00405 0.30 -2.1 75,541 + MMJJ_RS00405 0.30 +0.8 75,548 - MMJJ_RS00405 0.32 +1.5 75,596 - MMJJ_RS00405 0.40 -2.9 75,596 - MMJJ_RS00405 0.40 +1.9 75,616 - MMJJ_RS00405 0.43 +1.0 75,616 - MMJJ_RS00405 0.43 +1.1 75,685 + MMJJ_RS00405 0.55 -2.9 75,754 + MMJJ_RS00405 0.67 +1.4 75,781 + MMJJ_RS00405 0.72 -0.3 75,827 + MMJJ_RS00405 0.80 -1.2 75,941 - +0.4 75,942 + +0.9 75,943 - +1.6 75,946 - -1.3 75,947 + +0.8 75,966 - +1.3 75,976 - +1.9 76,026 - -2.4 76,030 + -0.2 76,077 - MMJJ_RS00410 0.21 -3.9 76,082 + MMJJ_RS00410 0.22 +1.4 76,085 + MMJJ_RS00410 0.22 +1.2 76,086 - MMJJ_RS00410 0.23 -1.9 76,086 - MMJJ_RS00410 0.23 -1.3 76,086 - MMJJ_RS00410 0.23 +1.5 76,086 - MMJJ_RS00410 0.23 -1.4 76,102 - MMJJ_RS00410 0.26 -3.3 76,152 - MMJJ_RS00410 0.38 -1.8 76,198 - MMJJ_RS00410 0.49 +1.4 76,198 - MMJJ_RS00410 0.49 -1.4 76,251 - MMJJ_RS00410 0.61 -2.8 76,252 + MMJJ_RS00410 0.61 -0.5 76,268 - MMJJ_RS00410 0.65 -2.5 76,268 - MMJJ_RS00410 0.65 +1.1 76,269 + MMJJ_RS00410 0.65 -1.1 76,269 + MMJJ_RS00410 0.65 -1.5 76,269 + MMJJ_RS00410 0.65 -0.9 76,269 + MMJJ_RS00410 0.65 -0.1 76,292 + MMJJ_RS00410 0.71 +1.2 76,292 + MMJJ_RS00410 0.71 -1.1 76,292 + MMJJ_RS00410 0.71 -1.2 76,299 + MMJJ_RS00410 0.72 -2.5 76,299 + MMJJ_RS00410 0.72 +0.6 76,416 + +1.0 76,416 + -1.7 76,416 + -0.7 76,424 + +1.9 76,458 + +1.3 76,458 + +0.5 76,463 + +1.1 76,463 + -3.2 76,482 - +0.3 76,483 + +2.2 76,513 + MMJJ_RS00415 0.11 +2.7 76,519 + MMJJ_RS00415 0.12 -2.1 76,582 + MMJJ_RS00415 0.25 +1.2 76,647 - MMJJ_RS00415 0.39 -1.7 76,647 - MMJJ_RS00415 0.39 -0.8 76,680 + MMJJ_RS00415 0.45 +1.8 76,751 - MMJJ_RS00415 0.60 +1.6 76,751 - MMJJ_RS00415 0.60 -1.8 76,763 - MMJJ_RS00415 0.63 -2.1 76,783 + MMJJ_RS00415 0.67 -1.5 76,833 + MMJJ_RS00415 0.77 -0.5 76,833 + MMJJ_RS00415 0.77 -1.8 76,836 - MMJJ_RS00415 0.78 -1.4 76,837 + MMJJ_RS00415 0.78 -0.4 76,837 + MMJJ_RS00415 0.78 +0.5 76,838 - MMJJ_RS00415 0.78 +0.6 76,839 + MMJJ_RS00415 0.78 +0.2 76,839 + MMJJ_RS00415 0.78 -0.6 76,839 + MMJJ_RS00415 0.78 +0.2 76,845 - MMJJ_RS00415 0.80 +1.2 76,903 - -1.5 76,908 + +0.7 76,908 + +1.7 76,911 - -0.5 76,982 - +2.2 76,998 + MMJJ_RS00420 0.10 +2.0 76,999 - MMJJ_RS00420 0.11 +1.4 77,030 - MMJJ_RS00420 0.19 -1.5 77,045 - MMJJ_RS00420 0.23 +0.3 77,108 - MMJJ_RS00420 0.39 -0.1 77,138 - MMJJ_RS00420 0.47 +0.6 77,138 - MMJJ_RS00420 0.47 +0.0 77,138 - MMJJ_RS00420 0.47 -1.3 77,139 + MMJJ_RS00420 0.48 +1.3 77,139 + MMJJ_RS00420 0.48 +0.2 77,199 + MMJJ_RS00420 0.63 +2.0 77,199 + MMJJ_RS00420 0.63 +1.9 77,199 + MMJJ_RS00420 0.63 +2.5 77,200 - MMJJ_RS00420 0.64 +2.1 77,222 - MMJJ_RS00420 0.69 +3.7 77,222 - MMJJ_RS00420 0.69 +0.9 77,223 + MMJJ_RS00420 0.70 +2.3 77,228 - MMJJ_RS00420 0.71 +1.0 77,229 + MMJJ_RS00420 0.71 +2.3 77,252 + MMJJ_RS00420 0.77 +2.9 77,262 + MMJJ_RS00420 0.80 +1.4 77,266 - MMJJ_RS00420 0.81 +0.6 77,367 - +2.1 77,370 - -3.3 77,370 - +1.6 77,371 + +2.0 77,372 - -1.4 77,372 - +0.9 77,373 + +1.6 77,373 + +1.6 77,374 - -0.7 77,374 - -0.9 77,375 + +0.3 77,379 + +0.6
Or see this region's nucleotide sequence