Experiment: R2A with Thallium(I) acetate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS14485 and CA265_RS14490 are separated by 173 nucleotides CA265_RS14490 and CA265_RS14495 are separated by 38 nucleotides CA265_RS14495 and CA265_RS14500 are separated by 255 nucleotides CA265_RS14500 and CA265_RS14505 overlap by 7 nucleotides CA265_RS14505 and CA265_RS14510 are separated by 78 nucleotides
CA265_RS14485: CA265_RS14485 - bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase, at 3,651,728 to 3,653,935
_RS14485
CA265_RS14490: CA265_RS14490 - transcriptional repressor, at 3,654,109 to 3,654,606
_RS14490
CA265_RS14495: CA265_RS14495 - adenylosuccinate synthase, at 3,654,645 to 3,655,925
_RS14495
CA265_RS14500: CA265_RS14500 - hypothetical protein, at 3,656,181 to 3,656,408
_RS14500
CA265_RS14505: CA265_RS14505 - hypothetical protein, at 3,656,402 to 3,656,698
_RS14505
CA265_RS14510: CA265_RS14510 - hypothetical protein, at 3,656,777 to 3,657,220
_RS14510
Position (kb)
3654
3655
3656 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3653.934 kb on - strand at 3653.937 kb on + strand at 3653.972 kb on - strand at 3654.035 kb on + strand at 3654.035 kb on + strand at 3654.080 kb on + strand at 3654.084 kb on + strand at 3654.614 kb on + strand at 3654.614 kb on + strand at 3655.926 kb on + strand at 3655.927 kb on - strand at 3655.965 kb on - strand at 3655.983 kb on + strand at 3655.984 kb on - strand at 3655.996 kb on - strand at 3656.076 kb on - strand at 3656.126 kb on - strand at 3656.168 kb on - strand at 3656.177 kb on + strand at 3656.190 kb on - strand at 3656.211 kb on - strand, within CA265_RS14500 at 3656.252 kb on - strand, within CA265_RS14500 at 3656.254 kb on + strand, within CA265_RS14500 at 3656.255 kb on - strand, within CA265_RS14500 at 3656.265 kb on - strand, within CA265_RS14500 at 3656.302 kb on + strand, within CA265_RS14500 at 3656.318 kb on + strand, within CA265_RS14500 at 3656.360 kb on + strand, within CA265_RS14500 at 3656.360 kb on + strand, within CA265_RS14500 at 3656.364 kb on + strand, within CA265_RS14500 at 3656.365 kb on - strand, within CA265_RS14500 at 3656.366 kb on + strand, within CA265_RS14500 at 3656.387 kb on - strand at 3656.464 kb on - strand at 3656.466 kb on - strand, within CA265_RS14505 at 3656.467 kb on + strand, within CA265_RS14505 at 3656.468 kb on - strand, within CA265_RS14505 at 3656.468 kb on - strand, within CA265_RS14505 at 3656.471 kb on + strand, within CA265_RS14505 at 3656.472 kb on - strand, within CA265_RS14505 at 3656.479 kb on + strand, within CA265_RS14505 at 3656.488 kb on + strand, within CA265_RS14505 at 3656.512 kb on + strand, within CA265_RS14505 at 3656.539 kb on + strand, within CA265_RS14505 at 3656.577 kb on - strand, within CA265_RS14505 at 3656.596 kb on + strand, within CA265_RS14505 at 3656.597 kb on - strand, within CA265_RS14505 at 3656.597 kb on - strand, within CA265_RS14505 at 3656.601 kb on - strand, within CA265_RS14505 at 3656.601 kb on - strand, within CA265_RS14505 at 3656.602 kb on - strand, within CA265_RS14505 at 3656.665 kb on - strand, within CA265_RS14505 at 3656.668 kb on - strand, within CA265_RS14505 at 3656.674 kb on + strand at 3656.696 kb on + strand at 3656.726 kb on + strand at 3656.910 kb on + strand, within CA265_RS14510
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Thallium(I) acetate 0.0001 mg/ml remove 3,653,934 - +0.1 3,653,937 + +1.0 3,653,972 - -0.6 3,654,035 + -0.3 3,654,035 + +0.8 3,654,080 + -0.5 3,654,084 + -0.6 3,654,614 + +0.6 3,654,614 + +1.0 3,655,926 + -0.4 3,655,927 - -1.8 3,655,965 - +0.6 3,655,983 + -2.3 3,655,984 - -1.3 3,655,996 - -0.7 3,656,076 - +0.9 3,656,126 - +0.6 3,656,168 - +0.5 3,656,177 + +1.0 3,656,190 - -0.0 3,656,211 - CA265_RS14500 0.13 -0.8 3,656,252 - CA265_RS14500 0.31 +1.4 3,656,254 + CA265_RS14500 0.32 +0.2 3,656,255 - CA265_RS14500 0.32 -1.0 3,656,265 - CA265_RS14500 0.37 -0.4 3,656,302 + CA265_RS14500 0.53 -0.3 3,656,318 + CA265_RS14500 0.60 -1.0 3,656,360 + CA265_RS14500 0.79 -2.8 3,656,360 + CA265_RS14500 0.79 -1.1 3,656,364 + CA265_RS14500 0.80 -0.2 3,656,365 - CA265_RS14500 0.81 -1.6 3,656,366 + CA265_RS14500 0.81 +0.3 3,656,387 - -0.2 3,656,464 - +0.3 3,656,466 - CA265_RS14505 0.22 -0.3 3,656,467 + CA265_RS14505 0.22 -0.9 3,656,468 - CA265_RS14505 0.22 -1.4 3,656,468 - CA265_RS14505 0.22 -0.0 3,656,471 + CA265_RS14505 0.23 +0.5 3,656,472 - CA265_RS14505 0.24 +0.7 3,656,479 + CA265_RS14505 0.26 +0.4 3,656,488 + CA265_RS14505 0.29 -0.1 3,656,512 + CA265_RS14505 0.37 +0.0 3,656,539 + CA265_RS14505 0.46 -0.5 3,656,577 - CA265_RS14505 0.59 +1.6 3,656,596 + CA265_RS14505 0.65 -0.1 3,656,597 - CA265_RS14505 0.66 +0.2 3,656,597 - CA265_RS14505 0.66 -0.5 3,656,601 - CA265_RS14505 0.67 -1.5 3,656,601 - CA265_RS14505 0.67 -0.1 3,656,602 - CA265_RS14505 0.67 +0.2 3,656,665 - CA265_RS14505 0.89 +0.2 3,656,668 - CA265_RS14505 0.90 +0.2 3,656,674 + +0.6 3,656,696 + +0.4 3,656,726 + -1.2 3,656,910 + CA265_RS14510 0.30 +0.7
Or see this region's nucleotide sequence