Experiment: R2A with Thallium(I) acetate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS06300 and CA265_RS06305 are separated by 295 nucleotides CA265_RS06305 and CA265_RS06310 are separated by 80 nucleotides CA265_RS06310 and CA265_RS06315 are separated by 137 nucleotides
CA265_RS06300: CA265_RS06300 - glucose dehydrogenase, at 1,547,922 to 1,549,010
_RS06300
CA265_RS06305: CA265_RS06305 - succinyl-CoA--3-ketoacid-CoA transferase, at 1,549,306 to 1,550,010
_RS06305
CA265_RS06310: CA265_RS06310 - succinyl-CoA--3-ketoacid-CoA transferase, at 1,550,091 to 1,550,750
_RS06310
CA265_RS06315: CA265_RS06315 - uridine kinase, at 1,550,888 to 1,551,532
_RS06315
Position (kb)
1549
1550
1551 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1548.432 kb on - strand, within CA265_RS06300 at 1548.460 kb on + strand, within CA265_RS06300 at 1548.475 kb on + strand, within CA265_RS06300 at 1548.476 kb on - strand, within CA265_RS06300 at 1548.481 kb on + strand, within CA265_RS06300 at 1548.487 kb on + strand, within CA265_RS06300 at 1548.497 kb on + strand, within CA265_RS06300 at 1548.581 kb on + strand, within CA265_RS06300 at 1548.627 kb on - strand, within CA265_RS06300 at 1548.652 kb on - strand, within CA265_RS06300 at 1548.666 kb on - strand, within CA265_RS06300 at 1548.680 kb on + strand, within CA265_RS06300 at 1548.716 kb on + strand, within CA265_RS06300 at 1548.790 kb on - strand, within CA265_RS06300 at 1548.790 kb on - strand, within CA265_RS06300 at 1548.909 kb on - strand at 1548.909 kb on - strand at 1548.912 kb on + strand at 1549.008 kb on + strand at 1549.008 kb on + strand at 1549.008 kb on + strand at 1549.146 kb on - strand at 1549.152 kb on - strand at 1549.152 kb on - strand at 1549.314 kb on + strand at 1549.314 kb on + strand at 1549.315 kb on - strand at 1549.315 kb on - strand at 1549.353 kb on - strand at 1549.464 kb on + strand, within CA265_RS06305 at 1549.537 kb on + strand, within CA265_RS06305 at 1549.538 kb on - strand, within CA265_RS06305 at 1549.538 kb on - strand, within CA265_RS06305 at 1549.592 kb on + strand at 1549.616 kb on + strand, within CA265_RS06305 at 1549.770 kb on + strand, within CA265_RS06305 at 1549.771 kb on - strand, within CA265_RS06305 at 1549.914 kb on + strand, within CA265_RS06305 at 1549.915 kb on - strand, within CA265_RS06305 at 1549.920 kb on + strand, within CA265_RS06305 at 1549.993 kb on - strand at 1550.051 kb on - strand at 1550.086 kb on + strand at 1550.086 kb on + strand at 1550.148 kb on + strand at 1550.148 kb on + strand at 1550.149 kb on - strand at 1550.149 kb on - strand at 1550.208 kb on - strand, within CA265_RS06310 at 1550.309 kb on + strand, within CA265_RS06310 at 1550.331 kb on - strand, within CA265_RS06310 at 1550.331 kb on - strand, within CA265_RS06310 at 1550.384 kb on - strand, within CA265_RS06310 at 1550.440 kb on + strand, within CA265_RS06310 at 1550.525 kb on + strand, within CA265_RS06310 at 1550.526 kb on - strand, within CA265_RS06310 at 1550.526 kb on - strand, within CA265_RS06310 at 1550.526 kb on - strand, within CA265_RS06310 at 1550.603 kb on - strand, within CA265_RS06310 at 1550.671 kb on + strand, within CA265_RS06310 at 1550.671 kb on + strand, within CA265_RS06310 at 1550.672 kb on - strand, within CA265_RS06310 at 1550.697 kb on - strand at 1550.714 kb on + strand at 1550.766 kb on + strand at 1550.794 kb on + strand at 1550.822 kb on + strand at 1550.834 kb on - strand at 1550.840 kb on - strand at 1550.886 kb on - strand at 1550.894 kb on + strand at 1550.895 kb on - strand at 1550.895 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Thallium(I) acetate 0.0001 mg/ml remove 1,548,432 - CA265_RS06300 0.47 -0.3 1,548,460 + CA265_RS06300 0.49 -0.2 1,548,475 + CA265_RS06300 0.51 +0.1 1,548,476 - CA265_RS06300 0.51 +1.7 1,548,481 + CA265_RS06300 0.51 -0.2 1,548,487 + CA265_RS06300 0.52 -1.2 1,548,497 + CA265_RS06300 0.53 +0.8 1,548,581 + CA265_RS06300 0.61 +0.4 1,548,627 - CA265_RS06300 0.65 -0.4 1,548,652 - CA265_RS06300 0.67 -0.0 1,548,666 - CA265_RS06300 0.68 +1.4 1,548,680 + CA265_RS06300 0.70 +0.3 1,548,716 + CA265_RS06300 0.73 -0.3 1,548,790 - CA265_RS06300 0.80 -0.7 1,548,790 - CA265_RS06300 0.80 -0.2 1,548,909 - -1.5 1,548,909 - +1.3 1,548,912 + +1.1 1,549,008 + +0.2 1,549,008 + +0.1 1,549,008 + +0.8 1,549,146 - -0.0 1,549,152 - -1.2 1,549,152 - -0.4 1,549,314 + +1.0 1,549,314 + +0.1 1,549,315 - +0.1 1,549,315 - +1.6 1,549,353 - -0.2 1,549,464 + CA265_RS06305 0.22 -1.6 1,549,537 + CA265_RS06305 0.33 +0.9 1,549,538 - CA265_RS06305 0.33 -1.0 1,549,538 - CA265_RS06305 0.33 +2.6 1,549,592 + +2.6 1,549,616 + CA265_RS06305 0.44 +0.6 1,549,770 + CA265_RS06305 0.66 -1.6 1,549,771 - CA265_RS06305 0.66 -0.8 1,549,914 + CA265_RS06305 0.86 +0.6 1,549,915 - CA265_RS06305 0.86 +2.1 1,549,920 + CA265_RS06305 0.87 +0.5 1,549,993 - -0.3 1,550,051 - -1.3 1,550,086 + +1.0 1,550,086 + +1.7 1,550,148 + +0.7 1,550,148 + -1.1 1,550,149 - +0.6 1,550,149 - -1.8 1,550,208 - CA265_RS06310 0.18 +0.1 1,550,309 + CA265_RS06310 0.33 +0.3 1,550,331 - CA265_RS06310 0.36 -0.7 1,550,331 - CA265_RS06310 0.36 +2.9 1,550,384 - CA265_RS06310 0.44 -0.6 1,550,440 + CA265_RS06310 0.53 +0.4 1,550,525 + CA265_RS06310 0.66 +0.2 1,550,526 - CA265_RS06310 0.66 +0.4 1,550,526 - CA265_RS06310 0.66 -1.5 1,550,526 - CA265_RS06310 0.66 -0.4 1,550,603 - CA265_RS06310 0.78 -0.8 1,550,671 + CA265_RS06310 0.88 +0.1 1,550,671 + CA265_RS06310 0.88 +0.3 1,550,672 - CA265_RS06310 0.88 -1.2 1,550,697 - -0.1 1,550,714 + -0.3 1,550,766 + +0.9 1,550,794 + -0.9 1,550,822 + +0.7 1,550,834 - +1.4 1,550,840 - +1.0 1,550,886 - +0.0 1,550,894 + +1.6 1,550,895 - -0.4 1,550,895 - -0.2
Or see this region's nucleotide sequence