Experiment: R2A with Thallium(I) acetate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS02875 and CA265_RS02880 are separated by 9 nucleotides CA265_RS02880 and CA265_RS02885 are separated by 375 nucleotides CA265_RS02885 and CA265_RS02890 are separated by 2 nucleotides CA265_RS02890 and CA265_RS02895 are separated by 215 nucleotides
CA265_RS02875: CA265_RS02875 - preprotein translocase subunit SecE, at 716,169 to 716,366
_RS02875
CA265_RS02880: CA265_RS02880 - transcription termination/antitermination factor NusG, at 716,376 to 716,915
_RS02880
CA265_RS02885: CA265_RS02885 - hypothetical protein, at 717,291 to 717,800
_RS02885
CA265_RS02890: CA265_RS02890 - hypothetical protein, at 717,803 to 718,321
_RS02890
CA265_RS02895: CA265_RS02895 - 50S ribosomal protein L11, at 718,537 to 718,980
_RS02895
Position (kb) 717
718 Strain fitness (log2 ratio) -2
-1
0
1
2 at 717.027 kb on - strand at 717.053 kb on - strand at 717.056 kb on + strand at 717.056 kb on + strand at 717.056 kb on + strand at 717.057 kb on - strand at 717.074 kb on - strand at 717.083 kb on + strand at 717.124 kb on + strand at 717.124 kb on + strand at 717.136 kb on - strand at 717.136 kb on - strand at 717.136 kb on - strand at 717.274 kb on - strand at 717.274 kb on - strand at 717.274 kb on - strand at 717.346 kb on + strand, within CA265_RS02885 at 717.346 kb on + strand, within CA265_RS02885 at 717.346 kb on + strand, within CA265_RS02885 at 717.349 kb on + strand, within CA265_RS02885 at 717.349 kb on + strand, within CA265_RS02885 at 717.402 kb on - strand, within CA265_RS02885 at 717.407 kb on + strand, within CA265_RS02885 at 717.407 kb on + strand, within CA265_RS02885 at 717.408 kb on - strand, within CA265_RS02885 at 717.429 kb on - strand, within CA265_RS02885 at 717.502 kb on + strand, within CA265_RS02885 at 717.503 kb on - strand, within CA265_RS02885 at 717.540 kb on + strand, within CA265_RS02885 at 717.542 kb on + strand, within CA265_RS02885 at 717.542 kb on + strand, within CA265_RS02885 at 717.574 kb on + strand, within CA265_RS02885 at 717.575 kb on - strand, within CA265_RS02885 at 717.575 kb on - strand, within CA265_RS02885 at 717.575 kb on - strand, within CA265_RS02885 at 717.601 kb on - strand, within CA265_RS02885 at 717.610 kb on - strand, within CA265_RS02885 at 717.616 kb on + strand, within CA265_RS02885 at 717.616 kb on + strand, within CA265_RS02885 at 717.663 kb on - strand, within CA265_RS02885 at 717.663 kb on - strand, within CA265_RS02885 at 717.664 kb on + strand, within CA265_RS02885 at 717.774 kb on - strand at 717.834 kb on + strand at 717.865 kb on - strand, within CA265_RS02890 at 717.952 kb on - strand, within CA265_RS02890 at 718.011 kb on + strand, within CA265_RS02890 at 718.012 kb on - strand, within CA265_RS02890 at 718.028 kb on - strand, within CA265_RS02890 at 718.062 kb on - strand, within CA265_RS02890 at 718.158 kb on - strand, within CA265_RS02890 at 718.205 kb on - strand, within CA265_RS02890 at 718.207 kb on - strand, within CA265_RS02890 at 718.210 kb on + strand, within CA265_RS02890 at 718.255 kb on - strand, within CA265_RS02890 at 718.257 kb on + strand, within CA265_RS02890 at 718.257 kb on + strand, within CA265_RS02890 at 718.258 kb on - strand, within CA265_RS02890 at 718.265 kb on + strand, within CA265_RS02890 at 718.267 kb on + strand, within CA265_RS02890 at 718.268 kb on - strand, within CA265_RS02890 at 718.275 kb on + strand at 718.276 kb on - strand at 718.283 kb on - strand at 718.300 kb on - strand at 718.306 kb on - strand at 718.306 kb on - strand at 718.308 kb on + strand at 718.320 kb on - strand at 718.349 kb on - strand at 718.383 kb on - strand at 718.389 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Thallium(I) acetate 0.0001 mg/ml remove 717,027 - -0.1 717,053 - +0.4 717,056 + +1.2 717,056 + -1.1 717,056 + -0.5 717,057 - -0.6 717,074 - -1.3 717,083 + -0.9 717,124 + +0.3 717,124 + +2.6 717,136 - +1.4 717,136 - -1.0 717,136 - -0.8 717,274 - +0.6 717,274 - -0.7 717,274 - +1.0 717,346 + CA265_RS02885 0.11 +0.8 717,346 + CA265_RS02885 0.11 -1.5 717,346 + CA265_RS02885 0.11 +0.3 717,349 + CA265_RS02885 0.11 -1.0 717,349 + CA265_RS02885 0.11 +0.2 717,402 - CA265_RS02885 0.22 -0.2 717,407 + CA265_RS02885 0.23 -0.7 717,407 + CA265_RS02885 0.23 +0.3 717,408 - CA265_RS02885 0.23 -0.5 717,429 - CA265_RS02885 0.27 +0.3 717,502 + CA265_RS02885 0.41 +0.4 717,503 - CA265_RS02885 0.42 -0.1 717,540 + CA265_RS02885 0.49 -1.1 717,542 + CA265_RS02885 0.49 -0.6 717,542 + CA265_RS02885 0.49 +0.8 717,574 + CA265_RS02885 0.55 -0.4 717,575 - CA265_RS02885 0.56 +0.3 717,575 - CA265_RS02885 0.56 +1.0 717,575 - CA265_RS02885 0.56 -0.2 717,601 - CA265_RS02885 0.61 -0.6 717,610 - CA265_RS02885 0.63 -0.4 717,616 + CA265_RS02885 0.64 -1.1 717,616 + CA265_RS02885 0.64 -0.6 717,663 - CA265_RS02885 0.73 +1.2 717,663 - CA265_RS02885 0.73 -2.2 717,664 + CA265_RS02885 0.73 +0.6 717,774 - -0.9 717,834 + +0.8 717,865 - CA265_RS02890 0.12 -0.2 717,952 - CA265_RS02890 0.29 -0.3 718,011 + CA265_RS02890 0.40 -0.7 718,012 - CA265_RS02890 0.40 -0.8 718,028 - CA265_RS02890 0.43 -0.5 718,062 - CA265_RS02890 0.50 -0.7 718,158 - CA265_RS02890 0.68 -0.6 718,205 - CA265_RS02890 0.77 -0.8 718,207 - CA265_RS02890 0.78 -0.8 718,210 + CA265_RS02890 0.78 +0.1 718,255 - CA265_RS02890 0.87 +0.8 718,257 + CA265_RS02890 0.87 -1.0 718,257 + CA265_RS02890 0.87 -0.9 718,258 - CA265_RS02890 0.88 -1.3 718,265 + CA265_RS02890 0.89 -1.3 718,267 + CA265_RS02890 0.89 +0.6 718,268 - CA265_RS02890 0.90 -0.1 718,275 + -1.0 718,276 - -0.7 718,283 - -0.7 718,300 - +0.5 718,306 - +1.0 718,306 - +0.2 718,308 + -0.6 718,320 - +0.7 718,349 - -0.2 718,383 - -0.2 718,389 + -1.1
Or see this region's nucleotide sequence