Experiment: L-Asparagine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS32795 and RR42_RS32800 are separated by 57 nucleotides RR42_RS32800 and RR42_RS32805 overlap by 68 nucleotides
RR42_RS32795: RR42_RS32795 - DNA polymerase III subunit alpha, at 2,751,623 to 2,755,069
_RS32795
RR42_RS32800: RR42_RS32800 - nucleotidyltransferase, at 2,755,127 to 2,756,683
_RS32800
RR42_RS32805: RR42_RS32805 - damage-inducible protein, at 2,756,616 to 2,757,512
_RS32805
Position (kb)
2755
2756
2757 Strain fitness (log2 ratio)
-1
0
1 at 2754.375 kb on + strand, within RR42_RS32795 at 2754.376 kb on - strand, within RR42_RS32795 at 2754.469 kb on - strand, within RR42_RS32795 at 2754.592 kb on + strand, within RR42_RS32795 at 2754.593 kb on - strand, within RR42_RS32795 at 2755.026 kb on + strand at 2755.026 kb on + strand at 2755.027 kb on - strand at 2755.236 kb on + strand at 2755.236 kb on + strand at 2755.236 kb on + strand at 2755.957 kb on - strand, within RR42_RS32800 at 2756.274 kb on + strand, within RR42_RS32800 at 2756.274 kb on + strand, within RR42_RS32800 at 2756.275 kb on - strand, within RR42_RS32800 at 2756.275 kb on - strand, within RR42_RS32800 at 2756.275 kb on - strand, within RR42_RS32800 at 2757.112 kb on + strand, within RR42_RS32805 at 2757.113 kb on - strand, within RR42_RS32805 at 2757.113 kb on - strand, within RR42_RS32805 at 2757.490 kb on - strand at 2757.534 kb on + strand at 2757.610 kb on - strand at 2757.643 kb on + strand at 2757.656 kb on + strand at 2757.656 kb on + strand at 2757.657 kb on - strand at 2757.657 kb on - strand at 2757.662 kb on + strand at 2757.662 kb on + strand at 2757.662 kb on + strand at 2757.663 kb on - strand at 2757.663 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Asparagine (C) remove 2,754,375 + RR42_RS32795 0.80 +0.2 2,754,376 - RR42_RS32795 0.80 -1.5 2,754,469 - RR42_RS32795 0.83 -1.2 2,754,592 + RR42_RS32795 0.86 -0.5 2,754,593 - RR42_RS32795 0.86 +0.9 2,755,026 + +1.7 2,755,026 + -0.3 2,755,027 - +0.3 2,755,236 + +0.5 2,755,236 + -0.4 2,755,236 + +0.5 2,755,957 - RR42_RS32800 0.53 -0.7 2,756,274 + RR42_RS32800 0.74 -0.2 2,756,274 + RR42_RS32800 0.74 -1.3 2,756,275 - RR42_RS32800 0.74 -0.7 2,756,275 - RR42_RS32800 0.74 -0.6 2,756,275 - RR42_RS32800 0.74 -1.7 2,757,112 + RR42_RS32805 0.55 -1.4 2,757,113 - RR42_RS32805 0.55 +0.3 2,757,113 - RR42_RS32805 0.55 +0.9 2,757,490 - -0.3 2,757,534 + -1.5 2,757,610 - +0.6 2,757,643 + +0.2 2,757,656 + +0.7 2,757,656 + -1.1 2,757,657 - +0.8 2,757,657 - +0.3 2,757,662 + +0.1 2,757,662 + -0.3 2,757,662 + +1.4 2,757,663 - -0.3 2,757,663 - +0.8
Or see this region's nucleotide sequence