Experiment: L-Asparagine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS21070 and RR42_RS21075 are separated by 409 nucleotides RR42_RS21075 and RR42_RS21080 are separated by 40 nucleotides RR42_RS21080 and RR42_RS21085 overlap by 4 nucleotides
RR42_RS21070: RR42_RS21070 - hypothetical protein, at 53,469 to 53,651
_RS21070
RR42_RS21075: RR42_RS21075 - phosphoribosyltransferase, at 54,061 to 54,768
_RS21075
RR42_RS21080: RR42_RS21080 - 3-isopropylmalate dehydratase, at 54,809 to 55,321
_RS21080
RR42_RS21085: RR42_RS21085 - 3-isopropylmalate dehydratase, at 55,318 to 56,625
_RS21085
Position (kb)
54
55 Strain fitness (log2 ratio)
-2
-1
0
1 at 53.100 kb on - strand at 53.172 kb on + strand at 53.207 kb on + strand at 53.207 kb on + strand at 53.208 kb on - strand at 53.208 kb on - strand at 53.249 kb on + strand at 53.332 kb on - strand at 53.470 kb on + strand at 53.471 kb on - strand at 53.489 kb on - strand, within RR42_RS21070 at 53.489 kb on - strand, within RR42_RS21070 at 53.522 kb on + strand, within RR42_RS21070 at 53.523 kb on - strand, within RR42_RS21070 at 53.523 kb on - strand, within RR42_RS21070 at 53.580 kb on + strand, within RR42_RS21070 at 53.581 kb on - strand, within RR42_RS21070 at 53.581 kb on - strand, within RR42_RS21070 at 53.645 kb on + strand at 53.645 kb on + strand at 53.646 kb on - strand at 53.646 kb on - strand at 53.646 kb on - strand at 53.776 kb on - strand at 53.794 kb on + strand at 53.794 kb on + strand at 53.795 kb on - strand at 53.795 kb on - strand at 53.795 kb on - strand at 53.947 kb on + strand at 53.947 kb on - strand at 53.948 kb on - strand at 53.949 kb on + strand at 53.949 kb on + strand at 53.949 kb on + strand at 53.950 kb on - strand at 53.950 kb on - strand at 53.950 kb on - strand at 53.950 kb on - strand at 54.691 kb on + strand, within RR42_RS21075 at 55.126 kb on + strand, within RR42_RS21080 at 55.228 kb on + strand, within RR42_RS21080 at 55.229 kb on - strand, within RR42_RS21080 at 55.229 kb on - strand, within RR42_RS21080 at 55.229 kb on - strand, within RR42_RS21080 at 55.229 kb on - strand, within RR42_RS21080 at 55.606 kb on + strand, within RR42_RS21085 at 55.606 kb on + strand, within RR42_RS21085 at 55.606 kb on + strand, within RR42_RS21085 at 55.606 kb on + strand, within RR42_RS21085 at 55.607 kb on - strand, within RR42_RS21085 at 55.607 kb on - strand, within RR42_RS21085 at 55.607 kb on - strand, within RR42_RS21085 at 55.697 kb on + strand, within RR42_RS21085 at 55.697 kb on + strand, within RR42_RS21085
Per-strain Table
Position Strand Gene LocusTag Fraction L-Asparagine (C) remove 53,100 - -0.7 53,172 + +0.9 53,207 + +0.3 53,207 + +1.5 53,208 - +0.3 53,208 - +0.3 53,249 + +0.1 53,332 - -0.3 53,470 + -1.1 53,471 - +0.5 53,489 - RR42_RS21070 0.11 +0.3 53,489 - RR42_RS21070 0.11 -0.5 53,522 + RR42_RS21070 0.29 +0.8 53,523 - RR42_RS21070 0.30 +1.2 53,523 - RR42_RS21070 0.30 -0.4 53,580 + RR42_RS21070 0.61 -0.2 53,581 - RR42_RS21070 0.61 +0.1 53,581 - RR42_RS21070 0.61 +0.1 53,645 + -0.1 53,645 + +0.2 53,646 - -0.1 53,646 - -0.7 53,646 - +0.4 53,776 - +1.2 53,794 + +0.9 53,794 + -1.1 53,795 - -0.9 53,795 - +0.6 53,795 - +0.5 53,947 + +0.8 53,947 - +0.4 53,948 - -0.0 53,949 + +0.6 53,949 + +1.0 53,949 + +0.1 53,950 - +0.3 53,950 - +0.1 53,950 - -0.5 53,950 - -1.0 54,691 + RR42_RS21075 0.89 -0.9 55,126 + RR42_RS21080 0.62 -0.0 55,228 + RR42_RS21080 0.82 +0.1 55,229 - RR42_RS21080 0.82 +0.4 55,229 - RR42_RS21080 0.82 +0.1 55,229 - RR42_RS21080 0.82 -0.6 55,229 - RR42_RS21080 0.82 -1.3 55,606 + RR42_RS21085 0.22 +1.0 55,606 + RR42_RS21085 0.22 -0.3 55,606 + RR42_RS21085 0.22 -0.7 55,606 + RR42_RS21085 0.22 -1.2 55,607 - RR42_RS21085 0.22 +0.8 55,607 - RR42_RS21085 0.22 +0.4 55,607 - RR42_RS21085 0.22 +0.4 55,697 + RR42_RS21085 0.29 -0.5 55,697 + RR42_RS21085 0.29 -1.8
Or see this region's nucleotide sequence