Experiment: KB with Trimethoprim 125 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_4253 and Psyr_4254 are separated by 625 nucleotides Psyr_4254 and Psyr_4255 are separated by 79 nucleotides Psyr_4255 and Psyr_4256 are separated by 154 nucleotides
Psyr_4253: Psyr_4253 - bacterial peptide chain release factor 3 (bRF-3), at 5,070,353 to 5,071,936
_4253
Psyr_4254: Psyr_4254 - conserved hypothetical protein, at 5,072,562 to 5,072,804
_4254
Psyr_4255: Psyr_4255 - FKBP-type peptidyl-prolyl isomerase, N-terminal:Peptidylprolyl isomerase, FKBP-type, at 5,072,884 to 5,073,501
_4255
Psyr_4256: Psyr_4256 - conserved hypothetical protein, at 5,073,656 to 5,073,964
_4256
Position (kb) 5072
5073 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 5072.008 kb on + strand at 5072.016 kb on + strand at 5072.016 kb on + strand at 5072.017 kb on - strand at 5072.017 kb on - strand at 5072.017 kb on - strand at 5072.017 kb on - strand at 5072.017 kb on - strand at 5072.077 kb on + strand at 5072.078 kb on - strand at 5072.078 kb on - strand at 5072.208 kb on + strand at 5072.208 kb on + strand at 5072.208 kb on + strand at 5072.208 kb on + strand at 5072.216 kb on + strand at 5072.216 kb on + strand at 5072.216 kb on + strand at 5072.217 kb on - strand at 5072.220 kb on + strand at 5072.220 kb on + strand at 5072.221 kb on - strand at 5072.257 kb on + strand at 5072.258 kb on - strand at 5072.258 kb on - strand at 5072.277 kb on + strand at 5072.277 kb on + strand at 5072.277 kb on + strand at 5072.278 kb on - strand at 5072.424 kb on + strand at 5072.425 kb on - strand at 5072.512 kb on + strand at 5072.512 kb on + strand at 5072.512 kb on + strand at 5072.512 kb on + strand at 5072.512 kb on + strand at 5072.512 kb on + strand at 5072.512 kb on + strand at 5072.513 kb on - strand at 5072.513 kb on - strand at 5072.568 kb on + strand at 5072.568 kb on + strand at 5072.568 kb on + strand at 5072.568 kb on + strand at 5072.568 kb on + strand at 5072.568 kb on + strand at 5072.569 kb on - strand at 5072.569 kb on - strand at 5072.569 kb on - strand at 5072.569 kb on - strand at 5072.569 kb on - strand at 5072.569 kb on - strand at 5072.569 kb on - strand at 5072.569 kb on - strand at 5072.569 kb on - strand at 5072.569 kb on - strand at 5072.569 kb on - strand at 5072.569 kb on - strand at 5072.605 kb on - strand, within Psyr_4254 at 5072.674 kb on + strand, within Psyr_4254 at 5072.767 kb on - strand, within Psyr_4254 at 5072.945 kb on - strand at 5072.945 kb on - strand at 5072.960 kb on + strand, within Psyr_4255 at 5073.080 kb on + strand, within Psyr_4255 at 5073.081 kb on - strand, within Psyr_4255 at 5073.081 kb on - strand, within Psyr_4255 at 5073.122 kb on + strand, within Psyr_4255 at 5073.134 kb on - strand, within Psyr_4255 at 5073.242 kb on + strand, within Psyr_4255 at 5073.459 kb on - strand at 5073.525 kb on - strand at 5073.525 kb on - strand at 5073.525 kb on - strand at 5073.525 kb on - strand at 5073.577 kb on - strand at 5073.577 kb on - strand at 5073.577 kb on - strand at 5073.604 kb on + strand at 5073.628 kb on + strand at 5073.628 kb on + strand at 5073.629 kb on - strand at 5073.644 kb on - strand at 5073.757 kb on - strand, within Psyr_4256 at 5073.773 kb on + strand, within Psyr_4256 at 5073.773 kb on + strand, within Psyr_4256 at 5073.774 kb on - strand, within Psyr_4256
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Trimethoprim 125 ug/ml remove 5,072,008 + -1.8 5,072,016 + +0.1 5,072,016 + -0.2 5,072,017 - +0.9 5,072,017 - -0.6 5,072,017 - +1.3 5,072,017 - +0.1 5,072,017 - +1.2 5,072,077 + -0.4 5,072,078 - -0.3 5,072,078 - +0.2 5,072,208 + -1.7 5,072,208 + -2.6 5,072,208 + -0.6 5,072,208 + +0.3 5,072,216 + -0.4 5,072,216 + +0.6 5,072,216 + +0.3 5,072,217 - +3.5 5,072,220 + +0.4 5,072,220 + +0.1 5,072,221 - -0.0 5,072,257 + -0.6 5,072,258 - +0.2 5,072,258 - +1.6 5,072,277 + -0.1 5,072,277 + -2.4 5,072,277 + +0.9 5,072,278 - +0.0 5,072,424 + -1.6 5,072,425 - -0.3 5,072,512 + +0.2 5,072,512 + -0.1 5,072,512 + -1.1 5,072,512 + +0.3 5,072,512 + -0.1 5,072,512 + +2.5 5,072,512 + +0.5 5,072,513 - -0.3 5,072,513 - -1.1 5,072,568 + -0.1 5,072,568 + +0.0 5,072,568 + +1.6 5,072,568 + +0.3 5,072,568 + -0.6 5,072,568 + +2.0 5,072,569 - +0.5 5,072,569 - +0.4 5,072,569 - +0.4 5,072,569 - -1.2 5,072,569 - +1.9 5,072,569 - +0.4 5,072,569 - +0.9 5,072,569 - -0.7 5,072,569 - +1.3 5,072,569 - -0.6 5,072,569 - +0.8 5,072,569 - -2.0 5,072,605 - Psyr_4254 0.18 -1.9 5,072,674 + Psyr_4254 0.46 -1.3 5,072,767 - Psyr_4254 0.84 -0.2 5,072,945 - +0.3 5,072,945 - +1.1 5,072,960 + Psyr_4255 0.12 -0.4 5,073,080 + Psyr_4255 0.32 +0.0 5,073,081 - Psyr_4255 0.32 -0.2 5,073,081 - Psyr_4255 0.32 +0.8 5,073,122 + Psyr_4255 0.39 +1.4 5,073,134 - Psyr_4255 0.40 +0.3 5,073,242 + Psyr_4255 0.58 +0.1 5,073,459 - -0.1 5,073,525 - +1.1 5,073,525 - -1.2 5,073,525 - +1.5 5,073,525 - -0.4 5,073,577 - +0.5 5,073,577 - +2.0 5,073,577 - -0.2 5,073,604 + +0.3 5,073,628 + +0.6 5,073,628 + -0.4 5,073,629 - -0.0 5,073,644 - +0.9 5,073,757 - Psyr_4256 0.33 +0.6 5,073,773 + Psyr_4256 0.38 -0.7 5,073,773 + Psyr_4256 0.38 -0.7 5,073,774 - Psyr_4256 0.38 -1.9
Or see this region's nucleotide sequence