Experiment: KB with Trimethoprim 125 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_4091 and Psyr_4092 are separated by 2 nucleotides Psyr_4092 and Psyr_4093 are separated by 57 nucleotides Psyr_4093 and Psyr_4094 are separated by 109 nucleotides
Psyr_4091: Psyr_4091 - 8-oxo-dGTPase, at 4,877,481 to 4,878,431
_4091
Psyr_4092: Psyr_4092 - Glutathione S-transferase, N-terminal, at 4,878,434 to 4,879,060
_4092
Psyr_4093: Psyr_4093 - glutamate N-acetyltransferase, at 4,879,118 to 4,880,335
_4093
Psyr_4094: Psyr_4094 - protein translocase subunit secA, at 4,880,445 to 4,883,186
_4094
Position (kb) 4879
4880
4881 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 4878.378 kb on + strand at 4878.486 kb on + strand at 4878.487 kb on - strand at 4878.487 kb on - strand at 4878.487 kb on - strand at 4878.487 kb on - strand at 4878.487 kb on - strand at 4878.487 kb on - strand at 4878.487 kb on - strand at 4878.487 kb on - strand at 4878.487 kb on - strand at 4878.501 kb on + strand, within Psyr_4092 at 4878.501 kb on + strand, within Psyr_4092 at 4878.502 kb on - strand, within Psyr_4092 at 4878.502 kb on - strand, within Psyr_4092 at 4878.502 kb on - strand, within Psyr_4092 at 4878.502 kb on - strand, within Psyr_4092 at 4878.502 kb on - strand, within Psyr_4092 at 4878.535 kb on - strand, within Psyr_4092 at 4878.725 kb on - strand, within Psyr_4092 at 4878.840 kb on + strand, within Psyr_4092 at 4878.841 kb on - strand, within Psyr_4092 at 4878.841 kb on - strand, within Psyr_4092 at 4878.915 kb on + strand, within Psyr_4092 at 4878.915 kb on + strand, within Psyr_4092 at 4878.915 kb on + strand, within Psyr_4092 at 4878.916 kb on - strand, within Psyr_4092 at 4878.916 kb on - strand, within Psyr_4092 at 4879.026 kb on + strand at 4879.027 kb on - strand at 4879.149 kb on + strand at 4879.149 kb on + strand at 4879.149 kb on + strand at 4879.149 kb on + strand at 4879.263 kb on + strand, within Psyr_4093 at 4879.263 kb on + strand, within Psyr_4093 at 4879.263 kb on + strand, within Psyr_4093 at 4879.263 kb on + strand, within Psyr_4093 at 4879.264 kb on - strand, within Psyr_4093 at 4879.264 kb on - strand, within Psyr_4093 at 4879.298 kb on + strand, within Psyr_4093 at 4879.298 kb on + strand, within Psyr_4093 at 4879.313 kb on + strand, within Psyr_4093 at 4879.313 kb on + strand, within Psyr_4093 at 4879.314 kb on - strand, within Psyr_4093 at 4879.372 kb on + strand, within Psyr_4093 at 4879.373 kb on - strand, within Psyr_4093 at 4879.373 kb on - strand, within Psyr_4093 at 4879.759 kb on - strand, within Psyr_4093 at 4880.053 kb on + strand, within Psyr_4093 at 4880.054 kb on - strand, within Psyr_4093 at 4880.122 kb on - strand, within Psyr_4093 at 4880.427 kb on + strand at 4880.427 kb on + strand at 4880.428 kb on - strand at 4880.428 kb on - strand at 4880.428 kb on - strand at 4880.428 kb on - strand at 4880.431 kb on - strand at 4880.431 kb on - strand at 4880.431 kb on - strand at 4880.446 kb on + strand at 4880.446 kb on + strand at 4880.447 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Trimethoprim 125 ug/ml remove 4,878,378 + -2.5 4,878,486 + +1.8 4,878,487 - +0.8 4,878,487 - -0.3 4,878,487 - -0.3 4,878,487 - +0.8 4,878,487 - -0.2 4,878,487 - -0.7 4,878,487 - +0.1 4,878,487 - +0.4 4,878,487 - +1.0 4,878,501 + Psyr_4092 0.11 +1.3 4,878,501 + Psyr_4092 0.11 -1.7 4,878,502 - Psyr_4092 0.11 +1.3 4,878,502 - Psyr_4092 0.11 +0.1 4,878,502 - Psyr_4092 0.11 +0.8 4,878,502 - Psyr_4092 0.11 -1.7 4,878,502 - Psyr_4092 0.11 -0.1 4,878,535 - Psyr_4092 0.16 +0.4 4,878,725 - Psyr_4092 0.46 -1.2 4,878,840 + Psyr_4092 0.65 +0.3 4,878,841 - Psyr_4092 0.65 +1.7 4,878,841 - Psyr_4092 0.65 +1.3 4,878,915 + Psyr_4092 0.77 -0.1 4,878,915 + Psyr_4092 0.77 +0.1 4,878,915 + Psyr_4092 0.77 -0.4 4,878,916 - Psyr_4092 0.77 -1.7 4,878,916 - Psyr_4092 0.77 -0.3 4,879,026 + -2.1 4,879,027 - +1.3 4,879,149 + +0.5 4,879,149 + -0.2 4,879,149 + -1.4 4,879,149 + -0.4 4,879,263 + Psyr_4093 0.12 -2.0 4,879,263 + Psyr_4093 0.12 -1.4 4,879,263 + Psyr_4093 0.12 -3.3 4,879,263 + Psyr_4093 0.12 -1.5 4,879,264 - Psyr_4093 0.12 -1.6 4,879,264 - Psyr_4093 0.12 -0.7 4,879,298 + Psyr_4093 0.15 -2.0 4,879,298 + Psyr_4093 0.15 +0.4 4,879,313 + Psyr_4093 0.16 -0.2 4,879,313 + Psyr_4093 0.16 -0.9 4,879,314 - Psyr_4093 0.16 -0.5 4,879,372 + Psyr_4093 0.21 -1.7 4,879,373 - Psyr_4093 0.21 +0.0 4,879,373 - Psyr_4093 0.21 -0.5 4,879,759 - Psyr_4093 0.53 -0.4 4,880,053 + Psyr_4093 0.77 +0.8 4,880,054 - Psyr_4093 0.77 +1.4 4,880,122 - Psyr_4093 0.82 +0.8 4,880,427 + -1.7 4,880,427 + -0.5 4,880,428 - +0.4 4,880,428 - +0.8 4,880,428 - +0.7 4,880,428 - +0.4 4,880,431 - +0.0 4,880,431 - -0.9 4,880,431 - -1.2 4,880,446 + +0.8 4,880,446 + -0.3 4,880,447 - +0.7
Or see this region's nucleotide sequence