Experiment: KB with Trimethoprim 125 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3738 and Psyr_3739 are separated by 177 nucleotides Psyr_3739 and Psyr_3740 are separated by 10 nucleotides Psyr_3740 and Psyr_3741 are separated by 38 nucleotides
Psyr_3738: Psyr_3738 - ATP-dependent DNA helicase RecQ, at 4,452,018 to 4,454,147
_3738
Psyr_3739: Psyr_3739 - conserved hypothetical protein, at 4,454,325 to 4,454,912
_3739
Psyr_3740: Psyr_3740 - Protein of unknown function DUF454, at 4,454,923 to 4,455,336
_3740
Psyr_3741: Psyr_3741 - Polyphosphate:AMP phosphotransferase, at 4,455,375 to 4,456,871
_3741
Position (kb) 4454
4455
4456 Strain fitness (log2 ratio) -2
-1
0
1
2 at 4454.072 kb on + strand at 4454.121 kb on - strand at 4454.124 kb on + strand at 4454.147 kb on + strand at 4454.148 kb on - strand at 4454.190 kb on + strand at 4454.190 kb on + strand at 4454.191 kb on - strand at 4454.191 kb on - strand at 4454.191 kb on - strand at 4454.192 kb on + strand at 4454.192 kb on + strand at 4454.243 kb on - strand at 4454.243 kb on - strand at 4454.287 kb on + strand at 4454.304 kb on + strand at 4454.315 kb on + strand at 4454.401 kb on - strand, within Psyr_3739 at 4454.401 kb on - strand, within Psyr_3739 at 4454.401 kb on - strand, within Psyr_3739 at 4454.499 kb on + strand, within Psyr_3739 at 4454.853 kb on + strand, within Psyr_3739 at 4455.097 kb on + strand, within Psyr_3740 at 4455.097 kb on + strand, within Psyr_3740 at 4455.145 kb on + strand, within Psyr_3740 at 4455.145 kb on + strand, within Psyr_3740 at 4455.146 kb on - strand, within Psyr_3740 at 4455.146 kb on - strand, within Psyr_3740 at 4455.184 kb on + strand, within Psyr_3740 at 4455.185 kb on - strand, within Psyr_3740 at 4455.289 kb on + strand, within Psyr_3740 at 4455.289 kb on + strand, within Psyr_3740 at 4455.289 kb on + strand, within Psyr_3740 at 4455.289 kb on + strand, within Psyr_3740 at 4455.289 kb on + strand, within Psyr_3740 at 4455.289 kb on + strand, within Psyr_3740 at 4455.290 kb on - strand, within Psyr_3740 at 4455.290 kb on - strand, within Psyr_3740 at 4455.290 kb on - strand, within Psyr_3740 at 4455.291 kb on + strand, within Psyr_3740 at 4455.292 kb on - strand, within Psyr_3740 at 4455.580 kb on + strand, within Psyr_3741 at 4455.580 kb on + strand, within Psyr_3741 at 4455.841 kb on + strand, within Psyr_3741 at 4455.842 kb on - strand, within Psyr_3741 at 4455.842 kb on - strand, within Psyr_3741 at 4455.893 kb on - strand, within Psyr_3741 at 4456.220 kb on - strand, within Psyr_3741 at 4456.313 kb on - strand, within Psyr_3741
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Trimethoprim 125 ug/ml remove 4,454,072 + -0.3 4,454,121 - -1.9 4,454,124 + -2.2 4,454,147 + +0.7 4,454,148 - +0.6 4,454,190 + +1.9 4,454,190 + -0.1 4,454,191 - +0.5 4,454,191 - -0.0 4,454,191 - -0.6 4,454,192 + +0.5 4,454,192 + +0.7 4,454,243 - +0.2 4,454,243 - -0.9 4,454,287 + -1.9 4,454,304 + +0.1 4,454,315 + -0.9 4,454,401 - Psyr_3739 0.13 +0.3 4,454,401 - Psyr_3739 0.13 -1.0 4,454,401 - Psyr_3739 0.13 -0.3 4,454,499 + Psyr_3739 0.30 +1.3 4,454,853 + Psyr_3739 0.90 -0.1 4,455,097 + Psyr_3740 0.42 -0.0 4,455,097 + Psyr_3740 0.42 -0.5 4,455,145 + Psyr_3740 0.54 -0.5 4,455,145 + Psyr_3740 0.54 -0.6 4,455,146 - Psyr_3740 0.54 -0.9 4,455,146 - Psyr_3740 0.54 -0.6 4,455,184 + Psyr_3740 0.63 -0.5 4,455,185 - Psyr_3740 0.63 -1.3 4,455,289 + Psyr_3740 0.88 +0.0 4,455,289 + Psyr_3740 0.88 -2.0 4,455,289 + Psyr_3740 0.88 -1.0 4,455,289 + Psyr_3740 0.88 -0.2 4,455,289 + Psyr_3740 0.88 +0.2 4,455,289 + Psyr_3740 0.88 -1.3 4,455,290 - Psyr_3740 0.89 -1.9 4,455,290 - Psyr_3740 0.89 +1.7 4,455,290 - Psyr_3740 0.89 -2.4 4,455,291 + Psyr_3740 0.89 -0.6 4,455,292 - Psyr_3740 0.89 +0.2 4,455,580 + Psyr_3741 0.14 -0.3 4,455,580 + Psyr_3741 0.14 -0.6 4,455,841 + Psyr_3741 0.31 -0.8 4,455,842 - Psyr_3741 0.31 +1.5 4,455,842 - Psyr_3741 0.31 +0.2 4,455,893 - Psyr_3741 0.35 +1.3 4,456,220 - Psyr_3741 0.56 +0.3 4,456,313 - Psyr_3741 0.63 -1.0
Or see this region's nucleotide sequence