Experiment: KB with Trimethoprim 125 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3437 and Psyr_3438 overlap by 4 nucleotides Psyr_3438 and Psyr_3439 are separated by 115 nucleotides Psyr_3439 and Psyr_3440 are separated by 11 nucleotides
Psyr_3437: Psyr_3437 - RNA polymerase, sigma 28 subunit, SigD/FliA/WhiG, at 4,100,576 to 4,101,316
_3437
Psyr_3438: Psyr_3438 - Cobyrinic acid a,c-diamide synthase, at 4,101,313 to 4,102,146
_3438
Psyr_3439: Psyr_3439 - GTP-binding signal recognition particle SRP54, G-domain protein, at 4,102,262 to 4,103,590
_3439
Psyr_3440: Psyr_3440 - Flagellar biosynthesis protein FlhA, at 4,103,602 to 4,105,731
_3440
Position (kb) 4102
4103
4104 Strain fitness (log2 ratio) -2
-1
0
1
2 at 4101.264 kb on + strand at 4101.291 kb on + strand at 4101.291 kb on + strand at 4101.291 kb on + strand at 4101.292 kb on - strand at 4101.292 kb on - strand at 4101.292 kb on - strand at 4101.292 kb on - strand at 4101.523 kb on + strand, within Psyr_3438 at 4101.523 kb on + strand, within Psyr_3438 at 4101.523 kb on + strand, within Psyr_3438 at 4101.524 kb on - strand, within Psyr_3438 at 4102.149 kb on + strand at 4102.150 kb on - strand at 4102.150 kb on - strand at 4102.250 kb on - strand at 4102.253 kb on - strand at 4102.328 kb on - strand at 4102.388 kb on - strand at 4102.425 kb on + strand, within Psyr_3439 at 4102.425 kb on + strand, within Psyr_3439 at 4102.437 kb on - strand, within Psyr_3439 at 4102.437 kb on - strand, within Psyr_3439 at 4102.530 kb on + strand, within Psyr_3439 at 4102.531 kb on - strand, within Psyr_3439 at 4102.587 kb on + strand, within Psyr_3439 at 4102.587 kb on + strand, within Psyr_3439 at 4102.588 kb on - strand, within Psyr_3439 at 4102.588 kb on - strand, within Psyr_3439 at 4102.588 kb on - strand, within Psyr_3439 at 4102.829 kb on - strand, within Psyr_3439 at 4102.842 kb on + strand, within Psyr_3439 at 4102.843 kb on - strand, within Psyr_3439 at 4102.854 kb on + strand, within Psyr_3439 at 4102.855 kb on - strand, within Psyr_3439 at 4103.599 kb on - strand at 4103.635 kb on + strand at 4103.636 kb on - strand at 4103.735 kb on - strand at 4103.878 kb on + strand, within Psyr_3440 at 4103.879 kb on - strand, within Psyr_3440 at 4103.879 kb on - strand, within Psyr_3440 at 4103.932 kb on + strand, within Psyr_3440 at 4103.933 kb on - strand, within Psyr_3440 at 4104.058 kb on + strand, within Psyr_3440 at 4104.058 kb on + strand, within Psyr_3440 at 4104.058 kb on + strand, within Psyr_3440 at 4104.059 kb on - strand, within Psyr_3440 at 4104.215 kb on + strand, within Psyr_3440 at 4104.215 kb on + strand, within Psyr_3440 at 4104.269 kb on + strand, within Psyr_3440
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Trimethoprim 125 ug/ml remove 4,101,264 + +0.0 4,101,291 + +0.5 4,101,291 + -0.4 4,101,291 + -0.8 4,101,292 - +1.8 4,101,292 - -0.6 4,101,292 - -0.2 4,101,292 - +0.3 4,101,523 + Psyr_3438 0.25 -0.3 4,101,523 + Psyr_3438 0.25 -0.2 4,101,523 + Psyr_3438 0.25 -1.6 4,101,524 - Psyr_3438 0.25 -0.6 4,102,149 + -1.6 4,102,150 - -0.9 4,102,150 - -1.1 4,102,250 - +0.5 4,102,253 - +0.2 4,102,328 - -0.7 4,102,388 - +0.6 4,102,425 + Psyr_3439 0.12 -2.5 4,102,425 + Psyr_3439 0.12 -2.1 4,102,437 - Psyr_3439 0.13 -0.9 4,102,437 - Psyr_3439 0.13 +0.2 4,102,530 + Psyr_3439 0.20 -2.3 4,102,531 - Psyr_3439 0.20 +0.0 4,102,587 + Psyr_3439 0.24 -0.7 4,102,587 + Psyr_3439 0.24 -0.2 4,102,588 - Psyr_3439 0.25 +0.7 4,102,588 - Psyr_3439 0.25 +0.2 4,102,588 - Psyr_3439 0.25 +0.3 4,102,829 - Psyr_3439 0.43 -0.7 4,102,842 + Psyr_3439 0.44 +0.7 4,102,843 - Psyr_3439 0.44 -0.8 4,102,854 + Psyr_3439 0.45 -2.1 4,102,855 - Psyr_3439 0.45 +0.2 4,103,599 - +0.1 4,103,635 + -0.3 4,103,636 - +0.3 4,103,735 - +0.2 4,103,878 + Psyr_3440 0.13 -0.7 4,103,879 - Psyr_3440 0.13 -0.5 4,103,879 - Psyr_3440 0.13 +0.2 4,103,932 + Psyr_3440 0.15 -1.3 4,103,933 - Psyr_3440 0.16 +0.7 4,104,058 + Psyr_3440 0.21 -1.8 4,104,058 + Psyr_3440 0.21 -1.3 4,104,058 + Psyr_3440 0.21 -0.4 4,104,059 - Psyr_3440 0.21 +0.3 4,104,215 + Psyr_3440 0.29 -0.3 4,104,215 + Psyr_3440 0.29 -0.3 4,104,269 + Psyr_3440 0.31 +0.3
Or see this region's nucleotide sequence