Experiment: KB with Trimethoprim 125 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3289 and Psyr_3290 are separated by 4 nucleotides Psyr_3290 and Psyr_3291 are separated by 301 nucleotides Psyr_3291 and Psyr_3292 are separated by 98 nucleotides
Psyr_3289: Psyr_3289 - 3-ketoacyl-CoA thiolase, at 3,935,596 to 3,936,771
_3289
Psyr_3290: Psyr_3290 - 3-hydroxyacyl-CoA dehydrogenase / short chain enoyl-CoA hydratase, at 3,936,776 to 3,938,941
_3290
Psyr_3291: Psyr_3291 - hypothetical protein, at 3,939,243 to 3,939,536
_3291
Psyr_3292: Psyr_3292 - conserved hypothetical protein, at 3,939,635 to 3,940,072
_3292
Position (kb) 3936
3937
3938
3939 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 3935.865 kb on + strand, within Psyr_3289 at 3935.866 kb on - strand, within Psyr_3289 at 3936.061 kb on + strand, within Psyr_3289 at 3936.061 kb on + strand, within Psyr_3289 at 3936.061 kb on + strand, within Psyr_3289 at 3936.672 kb on + strand at 3936.672 kb on + strand at 3936.672 kb on + strand at 3936.672 kb on + strand at 3936.672 kb on + strand at 3936.672 kb on + strand at 3936.673 kb on - strand at 3936.673 kb on - strand at 3936.673 kb on - strand at 3936.673 kb on - strand at 3936.762 kb on + strand at 3936.852 kb on + strand at 3936.852 kb on + strand at 3936.853 kb on - strand at 3936.964 kb on - strand at 3936.964 kb on - strand at 3936.964 kb on - strand at 3936.964 kb on - strand at 3937.093 kb on - strand, within Psyr_3290 at 3937.173 kb on + strand, within Psyr_3290 at 3937.174 kb on - strand, within Psyr_3290 at 3937.423 kb on - strand, within Psyr_3290 at 3937.789 kb on + strand, within Psyr_3290 at 3937.790 kb on - strand, within Psyr_3290 at 3937.931 kb on - strand, within Psyr_3290 at 3938.196 kb on + strand, within Psyr_3290 at 3938.926 kb on + strand at 3938.926 kb on + strand at 3938.927 kb on - strand at 3938.934 kb on + strand at 3938.934 kb on + strand at 3938.934 kb on + strand at 3938.935 kb on - strand at 3938.935 kb on - strand at 3939.060 kb on - strand at 3939.060 kb on - strand at 3939.106 kb on + strand at 3939.190 kb on + strand at 3939.190 kb on + strand at 3939.304 kb on + strand, within Psyr_3291 at 3939.304 kb on + strand, within Psyr_3291 at 3939.304 kb on + strand, within Psyr_3291 at 3939.304 kb on + strand, within Psyr_3291 at 3939.305 kb on - strand, within Psyr_3291 at 3939.305 kb on - strand, within Psyr_3291 at 3939.307 kb on + strand, within Psyr_3291 at 3939.307 kb on + strand, within Psyr_3291 at 3939.320 kb on - strand, within Psyr_3291 at 3939.374 kb on + strand, within Psyr_3291 at 3939.462 kb on + strand, within Psyr_3291 at 3939.462 kb on + strand, within Psyr_3291 at 3939.703 kb on - strand, within Psyr_3292 at 3939.738 kb on + strand, within Psyr_3292 at 3939.738 kb on + strand, within Psyr_3292 at 3939.765 kb on + strand, within Psyr_3292 at 3939.880 kb on - strand, within Psyr_3292
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Trimethoprim 125 ug/ml remove 3,935,865 + Psyr_3289 0.23 -3.0 3,935,866 - Psyr_3289 0.23 +0.1 3,936,061 + Psyr_3289 0.40 +0.6 3,936,061 + Psyr_3289 0.40 +1.2 3,936,061 + Psyr_3289 0.40 +0.6 3,936,672 + -0.8 3,936,672 + -0.2 3,936,672 + -0.3 3,936,672 + -1.0 3,936,672 + -0.7 3,936,672 + -0.5 3,936,673 - +0.2 3,936,673 - -0.8 3,936,673 - +1.5 3,936,673 - +0.4 3,936,762 + -0.5 3,936,852 + -1.4 3,936,852 + -1.4 3,936,853 - +1.2 3,936,964 - -0.4 3,936,964 - -1.5 3,936,964 - -1.0 3,936,964 - -0.0 3,937,093 - Psyr_3290 0.15 -0.2 3,937,173 + Psyr_3290 0.18 -1.2 3,937,174 - Psyr_3290 0.18 +1.2 3,937,423 - Psyr_3290 0.30 -0.4 3,937,789 + Psyr_3290 0.47 -2.1 3,937,790 - Psyr_3290 0.47 -1.4 3,937,931 - Psyr_3290 0.53 -0.6 3,938,196 + Psyr_3290 0.66 -0.6 3,938,926 + -2.8 3,938,926 + -0.9 3,938,927 - -0.5 3,938,934 + +0.3 3,938,934 + -1.0 3,938,934 + -1.0 3,938,935 - +0.4 3,938,935 - -0.2 3,939,060 - -0.8 3,939,060 - +0.5 3,939,106 + -0.6 3,939,190 + -0.1 3,939,190 + -0.4 3,939,304 + Psyr_3291 0.21 +0.6 3,939,304 + Psyr_3291 0.21 -0.0 3,939,304 + Psyr_3291 0.21 +0.6 3,939,304 + Psyr_3291 0.21 -0.4 3,939,305 - Psyr_3291 0.21 -0.8 3,939,305 - Psyr_3291 0.21 +0.4 3,939,307 + Psyr_3291 0.22 +0.8 3,939,307 + Psyr_3291 0.22 +0.4 3,939,320 - Psyr_3291 0.26 -0.6 3,939,374 + Psyr_3291 0.45 -1.0 3,939,462 + Psyr_3291 0.74 +0.1 3,939,462 + Psyr_3291 0.74 +1.0 3,939,703 - Psyr_3292 0.16 -0.4 3,939,738 + Psyr_3292 0.24 -1.7 3,939,738 + Psyr_3292 0.24 -2.8 3,939,765 + Psyr_3292 0.30 -3.1 3,939,880 - Psyr_3292 0.56 -1.4
Or see this region's nucleotide sequence