Experiment: KB with Trimethoprim 125 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3196 and Psyr_3197 are separated by 463 nucleotides Psyr_3197 and Psyr_3198 are separated by 10 nucleotides Psyr_3198 and Psyr_3199 are separated by 74 nucleotides
Psyr_3196: Psyr_3196 - isocitrate lyase, at 3,825,917 to 3,827,242
_3196
Psyr_3197: Psyr_3197 - NADH dehydrogenase subunit A, at 3,827,706 to 3,828,119
_3197
Psyr_3198: Psyr_3198 - NADH dehydrogenase subunit B, at 3,828,130 to 3,828,804
_3198
Psyr_3199: Psyr_3199 - NADH dehydrogenase subunit D / NADH dehydrogenase subunit C, at 3,828,879 to 3,830,660
_3199
Position (kb)
3828
3829 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3827.154 kb on - strand at 3827.332 kb on - strand at 3827.353 kb on + strand at 3827.355 kb on + strand at 3827.356 kb on - strand at 3827.369 kb on + strand at 3827.369 kb on + strand at 3827.371 kb on + strand at 3827.372 kb on - strand at 3827.372 kb on - strand at 3827.392 kb on - strand at 3827.417 kb on + strand at 3827.418 kb on - strand at 3827.464 kb on + strand at 3827.489 kb on + strand at 3827.490 kb on - strand at 3827.490 kb on - strand at 3827.493 kb on + strand at 3827.494 kb on - strand at 3828.045 kb on + strand, within Psyr_3197 at 3828.045 kb on + strand, within Psyr_3197 at 3828.136 kb on + strand at 3828.137 kb on - strand at 3828.241 kb on + strand, within Psyr_3198 at 3828.241 kb on + strand, within Psyr_3198 at 3828.241 kb on + strand, within Psyr_3198 at 3828.242 kb on - strand, within Psyr_3198 at 3828.334 kb on + strand, within Psyr_3198 at 3828.334 kb on + strand, within Psyr_3198 at 3828.482 kb on - strand, within Psyr_3198 at 3828.602 kb on - strand, within Psyr_3198 at 3828.766 kb on - strand at 3828.823 kb on + strand at 3828.878 kb on + strand at 3828.900 kb on + strand at 3828.900 kb on + strand at 3828.912 kb on + strand at 3828.912 kb on + strand at 3828.913 kb on - strand at 3829.158 kb on + strand, within Psyr_3199 at 3829.158 kb on + strand, within Psyr_3199 at 3829.231 kb on + strand, within Psyr_3199 at 3829.503 kb on + strand, within Psyr_3199 at 3829.504 kb on - strand, within Psyr_3199 at 3829.504 kb on - strand, within Psyr_3199 at 3829.720 kb on - strand, within Psyr_3199
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Trimethoprim 125 ug/ml remove 3,827,154 - -0.8 3,827,332 - +2.9 3,827,353 + -1.3 3,827,355 + +2.2 3,827,356 - +1.0 3,827,369 + +0.2 3,827,369 + -1.4 3,827,371 + -0.1 3,827,372 - -0.6 3,827,372 - +1.6 3,827,392 - +0.2 3,827,417 + -1.6 3,827,418 - -0.9 3,827,464 + -0.0 3,827,489 + -0.8 3,827,490 - -0.2 3,827,490 - +0.0 3,827,493 + -0.6 3,827,494 - +1.3 3,828,045 + Psyr_3197 0.82 -2.2 3,828,045 + Psyr_3197 0.82 -0.6 3,828,136 + -0.4 3,828,137 - -1.4 3,828,241 + Psyr_3198 0.16 -1.4 3,828,241 + Psyr_3198 0.16 -0.6 3,828,241 + Psyr_3198 0.16 +0.2 3,828,242 - Psyr_3198 0.17 -0.4 3,828,334 + Psyr_3198 0.30 -1.7 3,828,334 + Psyr_3198 0.30 +1.2 3,828,482 - Psyr_3198 0.52 +0.0 3,828,602 - Psyr_3198 0.70 +0.6 3,828,766 - -0.4 3,828,823 + +1.6 3,828,878 + -2.0 3,828,900 + -1.2 3,828,900 + -0.4 3,828,912 + -0.4 3,828,912 + +0.6 3,828,913 - -1.0 3,829,158 + Psyr_3199 0.16 -0.4 3,829,158 + Psyr_3199 0.16 +0.6 3,829,231 + Psyr_3199 0.20 -2.0 3,829,503 + Psyr_3199 0.35 -1.4 3,829,504 - Psyr_3199 0.35 -0.4 3,829,504 - Psyr_3199 0.35 -0.4 3,829,720 - Psyr_3199 0.47 +0.6
Or see this region's nucleotide sequence