Experiment: KB with Trimethoprim 125 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3177 and Psyr_3178 are separated by 18 nucleotides Psyr_3178 and Psyr_3179 are separated by 77 nucleotides Psyr_3179 and Psyr_3180 are separated by 468 nucleotides
Psyr_3177: Psyr_3177 - Recombination protein MgsA, at 3,803,963 to 3,805,285
_3177
Psyr_3178: Psyr_3178 - Outer membrane lipoprotein carrier protein LolA, at 3,805,304 to 3,805,927
_3178
Psyr_3179: Psyr_3179 - DNA translocase FtsK, at 3,806,005 to 3,808,410
_3179
Psyr_3180: Psyr_3180 - Leucyl/phenylalanyl-tRNA--protein transferase, at 3,808,879 to 3,809,568
_3180
Position (kb) 3806
3807
3808
3809 Strain fitness (log2 ratio) -2
-1
0
1
2 at 3805.193 kb on + strand at 3805.193 kb on + strand at 3805.193 kb on + strand at 3805.193 kb on + strand at 3805.193 kb on + strand at 3805.193 kb on + strand at 3805.193 kb on + strand at 3805.193 kb on + strand at 3805.193 kb on + strand at 3805.193 kb on + strand at 3805.193 kb on + strand at 3805.193 kb on + strand at 3805.193 kb on + strand at 3805.193 kb on + strand at 3805.193 kb on + strand at 3805.194 kb on - strand at 3805.194 kb on - strand at 3805.194 kb on - strand at 3805.194 kb on - strand at 3805.194 kb on - strand at 3805.194 kb on - strand at 3805.194 kb on - strand at 3805.194 kb on - strand at 3805.194 kb on - strand at 3805.194 kb on - strand at 3805.212 kb on + strand at 3805.212 kb on + strand at 3805.212 kb on + strand at 3805.212 kb on + strand at 3805.212 kb on + strand at 3805.212 kb on + strand at 3805.213 kb on - strand at 3805.213 kb on - strand at 3805.213 kb on - strand at 3805.213 kb on - strand at 3805.213 kb on - strand at 3805.213 kb on - strand at 3805.213 kb on - strand at 3805.213 kb on - strand at 3805.305 kb on + strand at 3805.305 kb on + strand at 3805.306 kb on - strand at 3806.404 kb on - strand, within Psyr_3179 at 3806.488 kb on - strand, within Psyr_3179 at 3806.721 kb on - strand, within Psyr_3179 at 3806.795 kb on - strand, within Psyr_3179 at 3806.795 kb on - strand, within Psyr_3179 at 3806.795 kb on - strand, within Psyr_3179 at 3806.795 kb on - strand, within Psyr_3179 at 3806.916 kb on - strand, within Psyr_3179 at 3806.916 kb on - strand, within Psyr_3179 at 3808.281 kb on - strand at 3808.505 kb on + strand at 3808.567 kb on - strand at 3808.575 kb on - strand at 3808.682 kb on - strand at 3808.682 kb on - strand at 3808.715 kb on - strand at 3809.198 kb on - strand, within Psyr_3180 at 3809.239 kb on + strand, within Psyr_3180 at 3809.240 kb on - strand, within Psyr_3180 at 3809.240 kb on - strand, within Psyr_3180 at 3809.271 kb on + strand, within Psyr_3180 at 3809.271 kb on + strand, within Psyr_3180 at 3809.272 kb on - strand, within Psyr_3180 at 3809.315 kb on - strand, within Psyr_3180
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Trimethoprim 125 ug/ml remove 3,805,193 + +1.0 3,805,193 + -1.6 3,805,193 + +1.0 3,805,193 + -0.2 3,805,193 + +0.4 3,805,193 + -0.6 3,805,193 + +1.0 3,805,193 + -0.7 3,805,193 + -1.2 3,805,193 + +0.0 3,805,193 + +0.1 3,805,193 + +0.2 3,805,193 + -1.0 3,805,193 + +0.0 3,805,193 + +0.0 3,805,194 - +0.0 3,805,194 - +0.1 3,805,194 - +1.1 3,805,194 - +0.4 3,805,194 - +0.4 3,805,194 - +0.6 3,805,194 - -0.4 3,805,194 - -2.0 3,805,194 - +0.2 3,805,194 - -0.1 3,805,212 + +0.2 3,805,212 + +0.6 3,805,212 + +0.4 3,805,212 + +0.1 3,805,212 + -0.2 3,805,212 + -0.7 3,805,213 - +2.5 3,805,213 - +0.2 3,805,213 - -0.2 3,805,213 - +0.2 3,805,213 - -0.2 3,805,213 - +0.4 3,805,213 - -2.2 3,805,213 - +1.2 3,805,305 + +0.0 3,805,305 + +1.9 3,805,306 - +0.3 3,806,404 - Psyr_3179 0.17 +0.2 3,806,488 - Psyr_3179 0.20 -0.4 3,806,721 - Psyr_3179 0.30 -1.0 3,806,795 - Psyr_3179 0.33 -1.7 3,806,795 - Psyr_3179 0.33 -1.0 3,806,795 - Psyr_3179 0.33 -0.6 3,806,795 - Psyr_3179 0.33 -1.0 3,806,916 - Psyr_3179 0.38 -1.4 3,806,916 - Psyr_3179 0.38 +0.2 3,808,281 - -1.0 3,808,505 + -1.7 3,808,567 - +1.6 3,808,575 - +0.1 3,808,682 - -1.6 3,808,682 - +0.6 3,808,715 - -1.3 3,809,198 - Psyr_3180 0.46 +0.4 3,809,239 + Psyr_3180 0.52 +0.2 3,809,240 - Psyr_3180 0.52 +0.5 3,809,240 - Psyr_3180 0.52 +0.2 3,809,271 + Psyr_3180 0.57 -1.0 3,809,271 + Psyr_3180 0.57 +1.6 3,809,272 - Psyr_3180 0.57 -0.1 3,809,315 - Psyr_3180 0.63 +0.9
Or see this region's nucleotide sequence