Experiment: KB with Trimethoprim 125 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3120 and Psyr_3121 are separated by 152 nucleotides Psyr_3121 and Psyr_3122 are separated by 42 nucleotides
Psyr_3120: Psyr_3120 - D-glucarate dehydratase, at 3,740,656 to 3,742,008
_3120
Psyr_3121: Psyr_3121 - Protein of unknown function DUF1345, at 3,742,161 to 3,742,814
_3121
Psyr_3122: Psyr_3122 - D-isomer specific 2-hydroxyacid dehydrogenase, NAD-binding protein, at 3,742,857 to 3,743,813
_3122
Position (kb) 3742
3743 Strain fitness (log2 ratio) -2
-1
0
1
2 at 3741.706 kb on - strand, within Psyr_3120 at 3741.907 kb on + strand at 3741.907 kb on + strand at 3741.908 kb on - strand at 3741.908 kb on - strand at 3742.104 kb on + strand at 3742.105 kb on - strand at 3742.105 kb on - strand at 3742.126 kb on + strand at 3742.127 kb on - strand at 3742.127 kb on - strand at 3742.127 kb on - strand at 3742.298 kb on - strand, within Psyr_3121 at 3742.578 kb on + strand, within Psyr_3121 at 3742.579 kb on - strand, within Psyr_3121 at 3742.579 kb on - strand, within Psyr_3121 at 3742.638 kb on + strand, within Psyr_3121 at 3742.654 kb on - strand, within Psyr_3121 at 3742.912 kb on + strand at 3742.913 kb on - strand at 3742.965 kb on + strand, within Psyr_3122 at 3742.976 kb on + strand, within Psyr_3122 at 3742.977 kb on - strand, within Psyr_3122 at 3742.977 kb on - strand, within Psyr_3122 at 3743.169 kb on + strand, within Psyr_3122 at 3743.169 kb on + strand, within Psyr_3122 at 3743.169 kb on + strand, within Psyr_3122 at 3743.169 kb on + strand, within Psyr_3122 at 3743.169 kb on + strand at 3743.170 kb on - strand, within Psyr_3122 at 3743.170 kb on - strand, within Psyr_3122 at 3743.170 kb on - strand, within Psyr_3122 at 3743.170 kb on - strand, within Psyr_3122 at 3743.170 kb on - strand, within Psyr_3122 at 3743.170 kb on - strand, within Psyr_3122 at 3743.170 kb on - strand, within Psyr_3122 at 3743.170 kb on - strand, within Psyr_3122 at 3743.171 kb on + strand, within Psyr_3122 at 3743.171 kb on + strand, within Psyr_3122 at 3743.171 kb on + strand, within Psyr_3122 at 3743.171 kb on + strand, within Psyr_3122 at 3743.171 kb on + strand, within Psyr_3122 at 3743.171 kb on + strand, within Psyr_3122 at 3743.172 kb on - strand, within Psyr_3122 at 3743.172 kb on - strand, within Psyr_3122 at 3743.330 kb on + strand, within Psyr_3122 at 3743.427 kb on + strand, within Psyr_3122 at 3743.427 kb on + strand, within Psyr_3122 at 3743.427 kb on + strand, within Psyr_3122 at 3743.427 kb on + strand, within Psyr_3122 at 3743.427 kb on + strand, within Psyr_3122 at 3743.427 kb on + strand, within Psyr_3122 at 3743.427 kb on + strand, within Psyr_3122 at 3743.427 kb on + strand, within Psyr_3122 at 3743.427 kb on + strand, within Psyr_3122 at 3743.427 kb on + strand, within Psyr_3122 at 3743.427 kb on + strand, within Psyr_3122 at 3743.428 kb on - strand, within Psyr_3122 at 3743.428 kb on - strand, within Psyr_3122 at 3743.428 kb on - strand, within Psyr_3122 at 3743.428 kb on - strand, within Psyr_3122 at 3743.428 kb on - strand, within Psyr_3122 at 3743.428 kb on - strand, within Psyr_3122 at 3743.428 kb on - strand, within Psyr_3122 at 3743.428 kb on - strand, within Psyr_3122 at 3743.428 kb on - strand, within Psyr_3122 at 3743.428 kb on - strand, within Psyr_3122 at 3743.428 kb on - strand, within Psyr_3122 at 3743.428 kb on - strand, within Psyr_3122 at 3743.428 kb on - strand, within Psyr_3122 at 3743.548 kb on - strand, within Psyr_3122 at 3743.719 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Trimethoprim 125 ug/ml remove 3,741,706 - Psyr_3120 0.78 +0.4 3,741,907 + +0.1 3,741,907 + +0.8 3,741,908 - -1.1 3,741,908 - -0.2 3,742,104 + -0.0 3,742,105 - +0.6 3,742,105 - -0.0 3,742,126 + +0.6 3,742,127 - -1.6 3,742,127 - -0.6 3,742,127 - +0.3 3,742,298 - Psyr_3121 0.21 -1.4 3,742,578 + Psyr_3121 0.64 -1.0 3,742,579 - Psyr_3121 0.64 -0.9 3,742,579 - Psyr_3121 0.64 +0.3 3,742,638 + Psyr_3121 0.73 -0.5 3,742,654 - Psyr_3121 0.75 -1.2 3,742,912 + +0.3 3,742,913 - +0.3 3,742,965 + Psyr_3122 0.11 -0.2 3,742,976 + Psyr_3122 0.12 +1.1 3,742,977 - Psyr_3122 0.13 -0.3 3,742,977 - Psyr_3122 0.13 +0.1 3,743,169 + Psyr_3122 0.33 +1.3 3,743,169 + Psyr_3122 0.33 +0.6 3,743,169 + Psyr_3122 0.33 +0.3 3,743,169 + Psyr_3122 0.33 +1.2 3,743,169 + +0.3 3,743,170 - Psyr_3122 0.33 -0.3 3,743,170 - Psyr_3122 0.33 -2.0 3,743,170 - Psyr_3122 0.33 -1.0 3,743,170 - Psyr_3122 0.33 +0.0 3,743,170 - Psyr_3122 0.33 -0.6 3,743,170 - Psyr_3122 0.33 +0.5 3,743,170 - Psyr_3122 0.33 +0.2 3,743,170 - Psyr_3122 0.33 -0.7 3,743,171 + Psyr_3122 0.33 +1.7 3,743,171 + Psyr_3122 0.33 -0.1 3,743,171 + Psyr_3122 0.33 -1.9 3,743,171 + Psyr_3122 0.33 -0.6 3,743,171 + Psyr_3122 0.33 +0.4 3,743,171 + Psyr_3122 0.33 -2.4 3,743,172 - Psyr_3122 0.33 +2.2 3,743,172 - Psyr_3122 0.33 -0.2 3,743,330 + Psyr_3122 0.49 -0.4 3,743,427 + Psyr_3122 0.60 +1.1 3,743,427 + Psyr_3122 0.60 -0.2 3,743,427 + Psyr_3122 0.60 -0.9 3,743,427 + Psyr_3122 0.60 +0.4 3,743,427 + Psyr_3122 0.60 +0.9 3,743,427 + Psyr_3122 0.60 -1.6 3,743,427 + Psyr_3122 0.60 +0.4 3,743,427 + Psyr_3122 0.60 +0.1 3,743,427 + Psyr_3122 0.60 -0.6 3,743,427 + Psyr_3122 0.60 -0.4 3,743,427 + Psyr_3122 0.60 +0.1 3,743,428 - Psyr_3122 0.60 -0.8 3,743,428 - Psyr_3122 0.60 -0.0 3,743,428 - Psyr_3122 0.60 +2.4 3,743,428 - Psyr_3122 0.60 +0.9 3,743,428 - Psyr_3122 0.60 +0.0 3,743,428 - Psyr_3122 0.60 -0.6 3,743,428 - Psyr_3122 0.60 -0.4 3,743,428 - Psyr_3122 0.60 -0.1 3,743,428 - Psyr_3122 0.60 +0.4 3,743,428 - Psyr_3122 0.60 -1.2 3,743,428 - Psyr_3122 0.60 -0.5 3,743,428 - Psyr_3122 0.60 +0.9 3,743,428 - Psyr_3122 0.60 -0.2 3,743,548 - Psyr_3122 0.72 -0.3 3,743,719 - +0.4
Or see this region's nucleotide sequence