Experiment: KB with Trimethoprim 125 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3089 and Psyr_3090 are separated by 5 nucleotides Psyr_3090 and Psyr_RNA46 are separated by 681 nucleotides Psyr_RNA46 and Psyr_3091 are separated by 207 nucleotides
Psyr_3089: Psyr_3089 - Hemolysin-type calcium-binding region:hemolysin-type calcium binding related protein, at 3,697,028 to 3,705,097
_3089
Psyr_3090: Psyr_3090 - hypothetical protein, at 3,705,103 to 3,705,765
_3090
Psyr_RNA46: Psyr_RNA46 - tRNA-Asn, at 3,706,447 to 3,706,522
_RNA46
Psyr_3091: Psyr_3091 - Response regulator receiver:Transcriptional regulatory protein, C-terminal, at 3,706,730 to 3,707,404
_3091
Position (kb)
3705
3706 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3704.192 kb on - strand, within Psyr_3089 at 3704.310 kb on + strand at 3704.334 kb on - strand at 3704.464 kb on + strand at 3704.509 kb on - strand at 3704.590 kb on + strand at 3704.685 kb on + strand at 3704.685 kb on + strand at 3704.706 kb on + strand at 3704.706 kb on + strand at 3704.716 kb on - strand at 3704.943 kb on + strand at 3705.010 kb on + strand at 3705.029 kb on + strand at 3705.239 kb on + strand, within Psyr_3090 at 3705.348 kb on + strand, within Psyr_3090 at 3705.551 kb on + strand, within Psyr_3090 at 3705.555 kb on + strand, within Psyr_3090 at 3705.556 kb on - strand, within Psyr_3090 at 3705.605 kb on + strand, within Psyr_3090 at 3705.621 kb on - strand, within Psyr_3090 at 3705.737 kb on + strand at 3705.820 kb on + strand at 3705.843 kb on - strand at 3705.858 kb on - strand at 3705.933 kb on - strand at 3705.958 kb on - strand at 3706.035 kb on + strand at 3706.055 kb on - strand at 3706.055 kb on - strand at 3706.216 kb on - strand at 3706.277 kb on + strand at 3706.278 kb on - strand at 3706.278 kb on - strand at 3706.279 kb on + strand at 3706.279 kb on + strand at 3706.344 kb on - strand at 3706.344 kb on - strand at 3706.352 kb on + strand at 3706.433 kb on + strand at 3706.434 kb on - strand at 3706.434 kb on - strand at 3706.434 kb on - strand at 3706.666 kb on + strand at 3706.666 kb on + strand at 3706.666 kb on + strand at 3706.666 kb on + strand at 3706.667 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Trimethoprim 125 ug/ml remove 3,704,192 - Psyr_3089 0.89 +0.2 3,704,310 + -0.4 3,704,334 - -1.0 3,704,464 + -4.1 3,704,509 - +1.0 3,704,590 + -4.1 3,704,685 + -0.9 3,704,685 + -0.4 3,704,706 + +0.6 3,704,706 + -2.2 3,704,716 - -1.9 3,704,943 + -1.4 3,705,010 + -0.4 3,705,029 + -1.0 3,705,239 + Psyr_3090 0.21 -1.0 3,705,348 + Psyr_3090 0.37 -2.2 3,705,551 + Psyr_3090 0.68 -1.2 3,705,555 + Psyr_3090 0.68 -1.9 3,705,556 - Psyr_3090 0.68 -1.8 3,705,605 + Psyr_3090 0.76 -0.5 3,705,621 - Psyr_3090 0.78 +0.6 3,705,737 + -0.3 3,705,820 + -0.4 3,705,843 - -0.0 3,705,858 - -0.2 3,705,933 - -0.4 3,705,958 - +0.6 3,706,035 + +2.4 3,706,055 - +0.6 3,706,055 - +0.2 3,706,216 - -1.3 3,706,277 + +1.0 3,706,278 - -1.7 3,706,278 - -0.4 3,706,279 + -1.8 3,706,279 + -1.4 3,706,344 - -2.6 3,706,344 - -0.2 3,706,352 + -2.0 3,706,433 + -1.4 3,706,434 - +1.0 3,706,434 - -1.1 3,706,434 - -1.0 3,706,666 + -0.1 3,706,666 + -0.8 3,706,666 + -0.7 3,706,666 + -0.1 3,706,667 - -2.2
Or see this region's nucleotide sequence