Experiment: KB with Trimethoprim 125 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2893 and Psyr_2894 are separated by 496 nucleotides Psyr_2894 and Psyr_RNA45 are separated by 91 nucleotides Psyr_RNA45 and Psyr_2895 are separated by 66 nucleotides Psyr_2895 and Psyr_2896 are separated by 32 nucleotides
Psyr_2893: Psyr_2893 - probable lipoprotein, at 3,472,035 to 3,472,703
_2893
Psyr_2894: Psyr_2894 - conserved hypothetical protein, at 3,473,200 to 3,473,658
_2894
Psyr_RNA45: Psyr_RNA45 - tRNA-Gly, at 3,473,750 to 3,473,825
_RNA45
Psyr_2895: Psyr_2895 - CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase, at 3,473,892 to 3,474,452
_2895
Psyr_2896: Psyr_2896 - Excinuclease ABC subunit C, at 3,474,485 to 3,476,308
_2896
Position (kb) 3473
3474 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 3472.205 kb on - strand, within Psyr_2893 at 3472.205 kb on - strand, within Psyr_2893 at 3472.232 kb on + strand, within Psyr_2893 at 3472.261 kb on + strand, within Psyr_2893 at 3472.261 kb on + strand, within Psyr_2893 at 3472.261 kb on + strand, within Psyr_2893 at 3472.261 kb on + strand, within Psyr_2893 at 3472.261 kb on + strand, within Psyr_2893 at 3472.262 kb on - strand, within Psyr_2893 at 3472.262 kb on - strand, within Psyr_2893 at 3472.262 kb on - strand, within Psyr_2893 at 3472.262 kb on - strand, within Psyr_2893 at 3472.352 kb on + strand, within Psyr_2893 at 3472.484 kb on - strand, within Psyr_2893 at 3472.516 kb on + strand, within Psyr_2893 at 3472.543 kb on + strand, within Psyr_2893 at 3472.544 kb on - strand, within Psyr_2893 at 3472.548 kb on - strand, within Psyr_2893 at 3472.548 kb on - strand, within Psyr_2893 at 3472.556 kb on + strand, within Psyr_2893 at 3472.557 kb on - strand, within Psyr_2893 at 3472.557 kb on - strand, within Psyr_2893 at 3472.605 kb on + strand, within Psyr_2893 at 3472.605 kb on + strand, within Psyr_2893 at 3472.692 kb on - strand at 3472.696 kb on + strand at 3472.697 kb on - strand at 3472.730 kb on - strand at 3472.816 kb on + strand at 3472.816 kb on + strand at 3472.876 kb on + strand at 3473.072 kb on + strand at 3473.073 kb on - strand at 3473.073 kb on - strand at 3473.073 kb on - strand at 3473.083 kb on + strand at 3473.083 kb on + strand at 3473.084 kb on - strand at 3473.105 kb on + strand at 3473.166 kb on + strand at 3473.166 kb on + strand at 3473.166 kb on + strand at 3473.167 kb on - strand at 3473.193 kb on - strand at 3473.203 kb on + strand at 3473.203 kb on + strand at 3473.234 kb on - strand at 3473.545 kb on + strand, within Psyr_2894 at 3473.546 kb on - strand, within Psyr_2894 at 3473.656 kb on + strand at 3473.656 kb on + strand at 3473.657 kb on - strand at 3473.657 kb on - strand at 3474.508 kb on - strand at 3474.508 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Trimethoprim 125 ug/ml remove 3,472,205 - Psyr_2893 0.25 +0.6 3,472,205 - Psyr_2893 0.25 -0.1 3,472,232 + Psyr_2893 0.29 -0.2 3,472,261 + Psyr_2893 0.34 -0.5 3,472,261 + Psyr_2893 0.34 +2.4 3,472,261 + Psyr_2893 0.34 +0.3 3,472,261 + Psyr_2893 0.34 -1.2 3,472,261 + Psyr_2893 0.34 -2.0 3,472,262 - Psyr_2893 0.34 +1.0 3,472,262 - Psyr_2893 0.34 -1.4 3,472,262 - Psyr_2893 0.34 +0.3 3,472,262 - Psyr_2893 0.34 -0.1 3,472,352 + Psyr_2893 0.47 -2.0 3,472,484 - Psyr_2893 0.67 -0.0 3,472,516 + Psyr_2893 0.72 +0.6 3,472,543 + Psyr_2893 0.76 -0.4 3,472,544 - Psyr_2893 0.76 -0.8 3,472,548 - Psyr_2893 0.77 +1.9 3,472,548 - Psyr_2893 0.77 +2.9 3,472,556 + Psyr_2893 0.78 +0.0 3,472,557 - Psyr_2893 0.78 -0.9 3,472,557 - Psyr_2893 0.78 -0.1 3,472,605 + Psyr_2893 0.85 -2.4 3,472,605 + Psyr_2893 0.85 -1.3 3,472,692 - +0.3 3,472,696 + +0.0 3,472,697 - -0.5 3,472,730 - +0.0 3,472,816 + +0.6 3,472,816 + -1.5 3,472,876 + -0.7 3,473,072 + +0.0 3,473,073 - +0.2 3,473,073 - -1.1 3,473,073 - +1.4 3,473,083 + -0.6 3,473,083 + +0.9 3,473,084 - -1.6 3,473,105 + -1.7 3,473,166 + +1.2 3,473,166 + +0.2 3,473,166 + -0.5 3,473,167 - -1.4 3,473,193 - +0.1 3,473,203 + -0.3 3,473,203 + -1.3 3,473,234 - -3.1 3,473,545 + Psyr_2894 0.75 -1.1 3,473,546 - Psyr_2894 0.75 -1.3 3,473,656 + +0.1 3,473,656 + -0.6 3,473,657 - +3.2 3,473,657 - +0.6 3,474,508 - -0.6 3,474,508 - -1.4
Or see this region's nucleotide sequence