Experiment: KB with Trimethoprim 125 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2345 and Psyr_2346 overlap by 4 nucleotides Psyr_2346 and Psyr_2347 are separated by 271 nucleotides
Psyr_2345: Psyr_2345 - ea59 protein, at 2,721,143 to 2,722,693
_2345
Psyr_2346: Psyr_2346 - conserved hypothetical protein, at 2,722,690 to 2,723,565
_2346
Psyr_2347: Psyr_2347 - hypothetical protein, at 2,723,837 to 2,724,484
_2347
Position (kb)
2722
2723
2724 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2721.767 kb on - strand, within Psyr_2345 at 2721.976 kb on + strand, within Psyr_2345 at 2722.039 kb on + strand, within Psyr_2345 at 2722.172 kb on - strand, within Psyr_2345 at 2722.272 kb on + strand, within Psyr_2345 at 2722.302 kb on + strand, within Psyr_2345 at 2722.347 kb on + strand, within Psyr_2345 at 2722.350 kb on + strand, within Psyr_2345 at 2722.350 kb on + strand, within Psyr_2345 at 2722.351 kb on - strand, within Psyr_2345 at 2722.351 kb on - strand, within Psyr_2345 at 2722.444 kb on - strand, within Psyr_2345 at 2722.447 kb on + strand, within Psyr_2345 at 2722.447 kb on + strand, within Psyr_2345 at 2722.597 kb on + strand at 2722.597 kb on + strand at 2722.598 kb on - strand at 2722.657 kb on + strand at 2722.687 kb on + strand at 2722.687 kb on + strand at 2722.688 kb on - strand at 2722.688 kb on - strand at 2722.688 kb on - strand at 2722.688 kb on - strand at 2722.689 kb on + strand at 2722.689 kb on + strand at 2722.689 kb on + strand at 2722.690 kb on - strand at 2722.690 kb on - strand at 2722.690 kb on - strand at 2722.694 kb on + strand at 2722.694 kb on + strand at 2722.694 kb on + strand at 2722.737 kb on + strand at 2722.738 kb on - strand at 2722.755 kb on - strand at 2722.769 kb on + strand at 2722.803 kb on - strand, within Psyr_2346 at 2722.822 kb on + strand, within Psyr_2346 at 2722.854 kb on + strand, within Psyr_2346 at 2722.854 kb on + strand, within Psyr_2346 at 2722.923 kb on - strand, within Psyr_2346 at 2722.923 kb on - strand, within Psyr_2346 at 2722.990 kb on - strand, within Psyr_2346 at 2723.070 kb on + strand, within Psyr_2346 at 2723.070 kb on + strand, within Psyr_2346 at 2723.075 kb on - strand, within Psyr_2346 at 2723.121 kb on + strand, within Psyr_2346 at 2723.121 kb on + strand, within Psyr_2346 at 2723.221 kb on + strand, within Psyr_2346 at 2723.222 kb on - strand, within Psyr_2346 at 2723.389 kb on + strand, within Psyr_2346 at 2723.389 kb on + strand, within Psyr_2346 at 2723.390 kb on - strand, within Psyr_2346 at 2723.396 kb on + strand, within Psyr_2346 at 2723.401 kb on - strand, within Psyr_2346 at 2723.401 kb on - strand, within Psyr_2346 at 2723.401 kb on - strand, within Psyr_2346 at 2723.442 kb on - strand, within Psyr_2346 at 2723.445 kb on + strand, within Psyr_2346 at 2723.446 kb on - strand, within Psyr_2346 at 2723.601 kb on + strand at 2723.602 kb on - strand at 2723.715 kb on + strand at 2723.761 kb on + strand at 2723.961 kb on - strand, within Psyr_2347 at 2723.970 kb on - strand, within Psyr_2347 at 2724.061 kb on - strand, within Psyr_2347 at 2724.128 kb on - strand, within Psyr_2347 at 2724.258 kb on - strand, within Psyr_2347 at 2724.258 kb on - strand, within Psyr_2347 at 2724.311 kb on + strand at 2724.471 kb on + strand at 2724.509 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Trimethoprim 125 ug/ml remove 2,721,767 - Psyr_2345 0.40 -0.6 2,721,976 + Psyr_2345 0.54 -0.6 2,722,039 + Psyr_2345 0.58 -1.0 2,722,172 - Psyr_2345 0.66 -2.0 2,722,272 + Psyr_2345 0.73 -1.0 2,722,302 + Psyr_2345 0.75 -2.2 2,722,347 + Psyr_2345 0.78 -0.8 2,722,350 + Psyr_2345 0.78 -0.7 2,722,350 + Psyr_2345 0.78 -0.3 2,722,351 - Psyr_2345 0.78 -1.2 2,722,351 - Psyr_2345 0.78 +0.4 2,722,444 - Psyr_2345 0.84 -1.7 2,722,447 + Psyr_2345 0.84 +1.6 2,722,447 + Psyr_2345 0.84 -1.2 2,722,597 + +0.7 2,722,597 + -0.3 2,722,598 - -4.1 2,722,657 + +1.5 2,722,687 + -1.1 2,722,687 + +0.8 2,722,688 - -2.4 2,722,688 - +0.6 2,722,688 - -3.6 2,722,688 - -2.0 2,722,689 + -0.2 2,722,689 + -0.8 2,722,689 + -0.0 2,722,690 - -2.9 2,722,690 - -1.0 2,722,690 - +2.2 2,722,694 + -2.4 2,722,694 + -1.1 2,722,694 + -0.4 2,722,737 + -1.2 2,722,738 - -3.5 2,722,755 - -2.7 2,722,769 + -1.6 2,722,803 - Psyr_2346 0.13 -2.4 2,722,822 + Psyr_2346 0.15 -0.5 2,722,854 + Psyr_2346 0.19 +1.3 2,722,854 + Psyr_2346 0.19 -0.2 2,722,923 - Psyr_2346 0.27 -2.2 2,722,923 - Psyr_2346 0.27 -1.9 2,722,990 - Psyr_2346 0.34 -1.4 2,723,070 + Psyr_2346 0.43 -1.4 2,723,070 + Psyr_2346 0.43 -1.4 2,723,075 - Psyr_2346 0.44 -1.9 2,723,121 + Psyr_2346 0.49 -0.3 2,723,121 + Psyr_2346 0.49 -1.9 2,723,221 + Psyr_2346 0.61 -0.0 2,723,222 - Psyr_2346 0.61 -0.6 2,723,389 + Psyr_2346 0.80 -2.4 2,723,389 + Psyr_2346 0.80 +0.3 2,723,390 - Psyr_2346 0.80 -2.0 2,723,396 + Psyr_2346 0.81 -2.6 2,723,401 - Psyr_2346 0.81 -3.1 2,723,401 - Psyr_2346 0.81 -3.4 2,723,401 - Psyr_2346 0.81 -2.6 2,723,442 - Psyr_2346 0.86 -0.0 2,723,445 + Psyr_2346 0.86 -0.4 2,723,446 - Psyr_2346 0.86 -2.2 2,723,601 + -1.4 2,723,602 - -2.7 2,723,715 + +0.2 2,723,761 + -1.4 2,723,961 - Psyr_2347 0.19 -1.7 2,723,970 - Psyr_2347 0.21 -0.4 2,724,061 - Psyr_2347 0.35 -2.7 2,724,128 - Psyr_2347 0.45 -2.0 2,724,258 - Psyr_2347 0.65 -2.0 2,724,258 - Psyr_2347 0.65 -2.1 2,724,311 + -1.7 2,724,471 + -1.4 2,724,509 - -3.2
Or see this region's nucleotide sequence