Experiment: KB with Trimethoprim 125 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2152 and Psyr_2153 overlap by 4 nucleotides Psyr_2153 and Psyr_2154 are separated by 1 nucleotides Psyr_2154 and Psyr_2155 are separated by 30 nucleotides Psyr_2155 and Psyr_2156 overlap by 4 nucleotides
Psyr_2152: Psyr_2152 - monosaccharide ABC transporter ATP-binding protein, CUT2 family, at 2,507,676 to 2,509,229
_2152
Psyr_2153: Psyr_2153 - monosaccharide ABC transporter membrane protein, CUT2 family, at 2,509,226 to 2,510,218
_2153
Psyr_2154: Psyr_2154 - transcriptional regulator, LacI family, at 2,510,220 to 2,511,236
_2154
Psyr_2155: Psyr_2155 - Carbohydrate kinase, PfkB, at 2,511,267 to 2,512,187
_2155
Psyr_2156: Psyr_2156 - RbsD or FucU transport, at 2,512,184 to 2,512,588
_2156
Position (kb)
2510
2511
2512 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2509.280 kb on + strand at 2509.280 kb on + strand at 2509.280 kb on + strand at 2509.281 kb on - strand at 2509.281 kb on - strand at 2509.281 kb on - strand at 2509.281 kb on - strand at 2509.281 kb on - strand at 2509.281 kb on - strand at 2509.295 kb on - strand at 2509.298 kb on + strand at 2509.298 kb on + strand at 2509.298 kb on + strand at 2509.298 kb on + strand at 2509.298 kb on + strand at 2509.298 kb on + strand at 2509.299 kb on - strand at 2509.299 kb on - strand at 2509.299 kb on - strand at 2509.299 kb on - strand at 2509.299 kb on - strand at 2509.299 kb on - strand at 2509.299 kb on - strand at 2509.697 kb on + strand, within Psyr_2153 at 2510.263 kb on - strand at 2510.352 kb on + strand, within Psyr_2154 at 2510.352 kb on + strand, within Psyr_2154 at 2510.353 kb on - strand, within Psyr_2154 at 2510.436 kb on + strand, within Psyr_2154 at 2510.437 kb on - strand, within Psyr_2154 at 2510.539 kb on - strand, within Psyr_2154 at 2510.629 kb on + strand, within Psyr_2154 at 2510.629 kb on + strand, within Psyr_2154 at 2510.630 kb on - strand, within Psyr_2154 at 2510.630 kb on - strand, within Psyr_2154 at 2510.630 kb on - strand, within Psyr_2154 at 2510.633 kb on + strand, within Psyr_2154 at 2510.633 kb on + strand, within Psyr_2154 at 2510.633 kb on + strand, within Psyr_2154 at 2510.633 kb on + strand at 2510.634 kb on - strand, within Psyr_2154 at 2510.712 kb on + strand, within Psyr_2154 at 2510.712 kb on + strand, within Psyr_2154 at 2510.712 kb on + strand, within Psyr_2154 at 2510.712 kb on + strand, within Psyr_2154 at 2510.712 kb on + strand, within Psyr_2154 at 2510.713 kb on - strand, within Psyr_2154 at 2510.763 kb on - strand, within Psyr_2154 at 2511.281 kb on - strand at 2511.286 kb on + strand at 2511.286 kb on + strand at 2511.287 kb on - strand at 2511.287 kb on - strand at 2511.289 kb on + strand at 2511.289 kb on + strand at 2511.289 kb on + strand at 2511.289 kb on + strand at 2511.289 kb on + strand at 2511.290 kb on - strand at 2511.290 kb on - strand at 2511.290 kb on - strand at 2511.290 kb on - strand at 2511.795 kb on + strand, within Psyr_2155 at 2511.796 kb on - strand, within Psyr_2155 at 2511.810 kb on + strand, within Psyr_2155 at 2511.980 kb on + strand, within Psyr_2155 at 2511.981 kb on - strand, within Psyr_2155 at 2511.981 kb on - strand, within Psyr_2155 at 2512.165 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Trimethoprim 125 ug/ml remove 2,509,280 + -0.5 2,509,280 + -0.6 2,509,280 + +0.0 2,509,281 - -0.3 2,509,281 - -0.3 2,509,281 - +1.2 2,509,281 - -1.7 2,509,281 - -2.1 2,509,281 - -0.1 2,509,295 - -0.1 2,509,298 + +1.6 2,509,298 + -0.6 2,509,298 + +0.9 2,509,298 + +0.6 2,509,298 + -1.2 2,509,298 + +0.2 2,509,299 - +1.6 2,509,299 - -1.2 2,509,299 - -1.6 2,509,299 - -0.4 2,509,299 - -0.9 2,509,299 - -1.3 2,509,299 - -0.7 2,509,697 + Psyr_2153 0.47 +1.4 2,510,263 - -1.0 2,510,352 + Psyr_2154 0.13 +2.5 2,510,352 + Psyr_2154 0.13 -2.6 2,510,353 - Psyr_2154 0.13 -0.2 2,510,436 + Psyr_2154 0.21 -0.1 2,510,437 - Psyr_2154 0.21 -1.0 2,510,539 - Psyr_2154 0.31 -0.7 2,510,629 + Psyr_2154 0.40 +0.1 2,510,629 + Psyr_2154 0.40 -1.2 2,510,630 - Psyr_2154 0.40 -1.4 2,510,630 - Psyr_2154 0.40 -0.5 2,510,630 - Psyr_2154 0.40 -1.1 2,510,633 + Psyr_2154 0.41 -0.7 2,510,633 + Psyr_2154 0.41 -0.4 2,510,633 + Psyr_2154 0.41 -0.2 2,510,633 + +2.2 2,510,634 - Psyr_2154 0.41 -2.0 2,510,712 + Psyr_2154 0.48 -1.4 2,510,712 + Psyr_2154 0.48 +1.4 2,510,712 + Psyr_2154 0.48 +1.3 2,510,712 + Psyr_2154 0.48 -1.3 2,510,712 + Psyr_2154 0.48 -1.0 2,510,713 - Psyr_2154 0.48 -0.7 2,510,763 - Psyr_2154 0.53 -1.7 2,511,281 - -0.5 2,511,286 + +1.3 2,511,286 + +0.8 2,511,287 - -0.9 2,511,287 - +0.4 2,511,289 + +0.4 2,511,289 + -0.5 2,511,289 + +0.6 2,511,289 + -0.1 2,511,289 + +2.2 2,511,290 - -0.7 2,511,290 - -1.0 2,511,290 - +1.6 2,511,290 - -0.4 2,511,795 + Psyr_2155 0.57 -0.4 2,511,796 - Psyr_2155 0.57 -1.5 2,511,810 + Psyr_2155 0.59 -0.3 2,511,980 + Psyr_2155 0.77 -0.2 2,511,981 - Psyr_2155 0.78 -0.8 2,511,981 - Psyr_2155 0.78 +2.9 2,512,165 + -1.4
Or see this region's nucleotide sequence