Experiment: KB with Trimethoprim 125 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1778 and Psyr_1779 are separated by 60 nucleotides Psyr_1779 and Psyr_1780 are separated by 152 nucleotides Psyr_1780 and Psyr_1781 are separated by 71 nucleotides
Psyr_1778: Psyr_1778 - PAS/PAC sensor hybrid histidine kinase, at 2,025,489 to 2,028,359
_1778
Psyr_1779: Psyr_1779 - hypothetical protein, at 2,028,420 to 2,028,719
_1779
Psyr_1780: Psyr_1780 - transcriptional regulator, TetR family, at 2,028,872 to 2,029,510
_1780
Psyr_1781: Psyr_1781 - Beta-glucosidase, at 2,029,582 to 2,032,302
_1781
Position (kb)
2028
2029
2030 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2027.987 kb on - strand, within Psyr_1778 at 2028.309 kb on - strand at 2028.419 kb on + strand at 2028.419 kb on + strand at 2028.419 kb on + strand at 2028.420 kb on - strand at 2028.521 kb on - strand, within Psyr_1779 at 2028.521 kb on - strand, within Psyr_1779 at 2028.521 kb on - strand, within Psyr_1779 at 2028.773 kb on + strand at 2028.773 kb on + strand at 2028.773 kb on + strand at 2028.774 kb on - strand at 2028.871 kb on + strand at 2028.871 kb on + strand at 2028.871 kb on + strand at 2028.871 kb on + strand at 2028.871 kb on + strand at 2028.871 kb on + strand at 2028.872 kb on - strand at 2028.872 kb on - strand at 2028.872 kb on - strand at 2028.872 kb on - strand at 2028.872 kb on - strand at 2028.872 kb on - strand at 2028.872 kb on - strand at 2028.886 kb on - strand at 2028.899 kb on - strand at 2028.992 kb on + strand, within Psyr_1780 at 2029.112 kb on + strand, within Psyr_1780 at 2029.113 kb on - strand, within Psyr_1780 at 2029.113 kb on - strand, within Psyr_1780 at 2029.452 kb on + strand at 2029.452 kb on + strand at 2029.453 kb on - strand at 2029.523 kb on + strand at 2029.524 kb on - strand at 2029.583 kb on + strand at 2029.586 kb on + strand at 2029.587 kb on - strand at 2029.679 kb on - strand at 2029.679 kb on - strand at 2029.775 kb on + strand at 2029.775 kb on + strand at 2029.775 kb on + strand at 2029.779 kb on - strand at 2029.839 kb on - strand at 2029.904 kb on + strand, within Psyr_1781 at 2029.904 kb on + strand, within Psyr_1781 at 2029.904 kb on + strand, within Psyr_1781 at 2029.904 kb on + strand, within Psyr_1781 at 2029.904 kb on + strand, within Psyr_1781 at 2029.905 kb on - strand, within Psyr_1781 at 2029.905 kb on - strand, within Psyr_1781 at 2030.016 kb on - strand, within Psyr_1781 at 2030.021 kb on + strand, within Psyr_1781 at 2030.021 kb on + strand, within Psyr_1781 at 2030.021 kb on + strand, within Psyr_1781 at 2030.022 kb on - strand, within Psyr_1781 at 2030.022 kb on - strand, within Psyr_1781 at 2030.022 kb on - strand, within Psyr_1781 at 2030.031 kb on - strand, within Psyr_1781 at 2030.031 kb on - strand, within Psyr_1781 at 2030.031 kb on - strand, within Psyr_1781 at 2030.114 kb on + strand, within Psyr_1781 at 2030.115 kb on - strand, within Psyr_1781 at 2030.115 kb on - strand, within Psyr_1781 at 2030.150 kb on + strand, within Psyr_1781 at 2030.150 kb on + strand, within Psyr_1781 at 2030.151 kb on - strand, within Psyr_1781 at 2030.151 kb on - strand, within Psyr_1781 at 2030.151 kb on - strand, within Psyr_1781 at 2030.172 kb on - strand, within Psyr_1781 at 2030.180 kb on + strand, within Psyr_1781 at 2030.180 kb on + strand, within Psyr_1781 at 2030.180 kb on + strand, within Psyr_1781 at 2030.181 kb on - strand, within Psyr_1781 at 2030.181 kb on - strand, within Psyr_1781 at 2030.181 kb on - strand, within Psyr_1781 at 2030.254 kb on + strand, within Psyr_1781 at 2030.254 kb on + strand, within Psyr_1781 at 2030.254 kb on + strand, within Psyr_1781 at 2030.255 kb on - strand, within Psyr_1781 at 2030.363 kb on - strand, within Psyr_1781 at 2030.498 kb on + strand, within Psyr_1781 at 2030.498 kb on + strand, within Psyr_1781
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Trimethoprim 125 ug/ml remove 2,027,987 - Psyr_1778 0.87 +0.3 2,028,309 - +0.4 2,028,419 + -1.0 2,028,419 + -0.4 2,028,419 + +0.1 2,028,420 - -1.2 2,028,521 - Psyr_1779 0.34 +0.1 2,028,521 - Psyr_1779 0.34 -0.2 2,028,521 - Psyr_1779 0.34 +0.5 2,028,773 + -1.1 2,028,773 + -0.3 2,028,773 + +0.2 2,028,774 - -1.1 2,028,871 + +0.9 2,028,871 + -0.2 2,028,871 + -1.1 2,028,871 + +1.0 2,028,871 + +0.1 2,028,871 + -0.4 2,028,872 - +1.3 2,028,872 - +0.7 2,028,872 - -0.5 2,028,872 - -1.0 2,028,872 - +1.2 2,028,872 - -1.3 2,028,872 - -0.5 2,028,886 - +0.7 2,028,899 - -0.3 2,028,992 + Psyr_1780 0.19 -2.3 2,029,112 + Psyr_1780 0.38 -1.6 2,029,113 - Psyr_1780 0.38 +1.7 2,029,113 - Psyr_1780 0.38 -0.0 2,029,452 + -2.0 2,029,452 + +1.1 2,029,453 - -1.1 2,029,523 + +0.7 2,029,524 - -0.2 2,029,583 + +1.0 2,029,586 + -2.6 2,029,587 - +1.1 2,029,679 - -0.7 2,029,679 - +2.3 2,029,775 + -0.8 2,029,775 + -0.3 2,029,775 + -1.4 2,029,779 - -0.2 2,029,839 - +0.5 2,029,904 + Psyr_1781 0.12 +0.3 2,029,904 + Psyr_1781 0.12 +0.7 2,029,904 + Psyr_1781 0.12 -0.7 2,029,904 + Psyr_1781 0.12 +0.1 2,029,904 + Psyr_1781 0.12 -0.1 2,029,905 - Psyr_1781 0.12 +1.4 2,029,905 - Psyr_1781 0.12 -0.3 2,030,016 - Psyr_1781 0.16 +0.2 2,030,021 + Psyr_1781 0.16 -0.1 2,030,021 + Psyr_1781 0.16 +0.1 2,030,021 + Psyr_1781 0.16 +0.1 2,030,022 - Psyr_1781 0.16 +0.2 2,030,022 - Psyr_1781 0.16 -0.4 2,030,022 - Psyr_1781 0.16 +2.0 2,030,031 - Psyr_1781 0.17 +0.5 2,030,031 - Psyr_1781 0.17 +0.1 2,030,031 - Psyr_1781 0.17 +1.0 2,030,114 + Psyr_1781 0.20 +0.4 2,030,115 - Psyr_1781 0.20 +0.4 2,030,115 - Psyr_1781 0.20 -1.9 2,030,150 + Psyr_1781 0.21 -1.3 2,030,150 + Psyr_1781 0.21 -0.2 2,030,151 - Psyr_1781 0.21 +0.4 2,030,151 - Psyr_1781 0.21 +0.1 2,030,151 - Psyr_1781 0.21 +0.3 2,030,172 - Psyr_1781 0.22 +0.4 2,030,180 + Psyr_1781 0.22 -0.4 2,030,180 + Psyr_1781 0.22 +0.1 2,030,180 + Psyr_1781 0.22 +0.2 2,030,181 - Psyr_1781 0.22 +0.7 2,030,181 - Psyr_1781 0.22 +0.2 2,030,181 - Psyr_1781 0.22 -0.3 2,030,254 + Psyr_1781 0.25 -0.9 2,030,254 + Psyr_1781 0.25 -1.1 2,030,254 + Psyr_1781 0.25 +0.5 2,030,255 - Psyr_1781 0.25 -0.7 2,030,363 - Psyr_1781 0.29 +0.2 2,030,498 + Psyr_1781 0.34 -0.1 2,030,498 + Psyr_1781 0.34 -1.3
Or see this region's nucleotide sequence