Experiment: KB with Trimethoprim 125 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1555 and Psyr_1556 are separated by 373 nucleotides
Psyr_1555: Psyr_1555 - Cobyrinic acid a,c-diamide synthase, at 1,748,828 to 1,749,769
_1555
Psyr_1556: Psyr_1556 - hypothetical protein, at 1,750,143 to 1,750,403
_1556
Position (kb) 1750
1751 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 1749.150 kb on + strand, within Psyr_1555 at 1749.234 kb on + strand, within Psyr_1555 at 1749.234 kb on + strand, within Psyr_1555 at 1749.234 kb on + strand, within Psyr_1555 at 1749.234 kb on + strand, within Psyr_1555 at 1749.235 kb on - strand, within Psyr_1555 at 1749.276 kb on - strand, within Psyr_1555 at 1749.433 kb on + strand, within Psyr_1555 at 1749.456 kb on - strand, within Psyr_1555 at 1749.493 kb on + strand, within Psyr_1555 at 1749.494 kb on - strand, within Psyr_1555 at 1749.598 kb on + strand, within Psyr_1555 at 1749.604 kb on - strand at 1749.604 kb on - strand, within Psyr_1555 at 1749.633 kb on - strand, within Psyr_1555 at 1749.690 kb on - strand at 1749.701 kb on + strand at 1749.701 kb on + strand at 1749.769 kb on + strand at 1749.769 kb on + strand at 1749.770 kb on - strand at 1749.770 kb on - strand at 1749.773 kb on + strand at 1749.773 kb on + strand at 1749.780 kb on - strand at 1749.797 kb on + strand at 1749.815 kb on - strand at 1749.879 kb on + strand at 1749.950 kb on + strand at 1749.973 kb on + strand at 1750.296 kb on - strand, within Psyr_1556 at 1750.307 kb on + strand, within Psyr_1556 at 1750.307 kb on + strand, within Psyr_1556 at 1750.311 kb on + strand, within Psyr_1556 at 1750.361 kb on + strand, within Psyr_1556 at 1750.376 kb on + strand, within Psyr_1556 at 1750.419 kb on + strand at 1750.446 kb on + strand at 1750.530 kb on + strand at 1750.530 kb on + strand at 1750.649 kb on + strand at 1750.649 kb on + strand at 1750.684 kb on - strand at 1750.870 kb on + strand at 1750.946 kb on - strand at 1751.066 kb on + strand at 1751.067 kb on - strand at 1751.151 kb on - strand at 1751.151 kb on - strand at 1751.185 kb on - strand at 1751.194 kb on + strand at 1751.194 kb on + strand at 1751.270 kb on - strand at 1751.280 kb on + strand at 1751.281 kb on - strand at 1751.326 kb on + strand at 1751.326 kb on + strand at 1751.327 kb on - strand at 1751.383 kb on - strand at 1751.399 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Trimethoprim 125 ug/ml remove 1,749,150 + Psyr_1555 0.34 -0.2 1,749,234 + Psyr_1555 0.43 -4.0 1,749,234 + Psyr_1555 0.43 -1.2 1,749,234 + Psyr_1555 0.43 -2.5 1,749,234 + Psyr_1555 0.43 -1.9 1,749,235 - Psyr_1555 0.43 -1.2 1,749,276 - Psyr_1555 0.48 -2.1 1,749,433 + Psyr_1555 0.64 -2.7 1,749,456 - Psyr_1555 0.67 +2.8 1,749,493 + Psyr_1555 0.71 -0.4 1,749,494 - Psyr_1555 0.71 -1.3 1,749,598 + Psyr_1555 0.82 -2.2 1,749,604 - +0.2 1,749,604 - Psyr_1555 0.82 -1.5 1,749,633 - Psyr_1555 0.85 -1.7 1,749,690 - -2.0 1,749,701 + -0.8 1,749,701 + -2.6 1,749,769 + -3.2 1,749,769 + -3.0 1,749,770 - +0.2 1,749,770 - -1.4 1,749,773 + -0.2 1,749,773 + -1.5 1,749,780 - -0.8 1,749,797 + +0.8 1,749,815 - +0.1 1,749,879 + -3.0 1,749,950 + -1.5 1,749,973 + -0.9 1,750,296 - Psyr_1556 0.59 -2.5 1,750,307 + Psyr_1556 0.63 -2.4 1,750,307 + Psyr_1556 0.63 -1.5 1,750,311 + Psyr_1556 0.64 +0.8 1,750,361 + Psyr_1556 0.84 -2.4 1,750,376 + Psyr_1556 0.89 -1.0 1,750,419 + -3.0 1,750,446 + -0.4 1,750,530 + -0.5 1,750,530 + -3.8 1,750,649 + -1.8 1,750,649 + -1.8 1,750,684 - -1.5 1,750,870 + -1.8 1,750,946 - -2.0 1,751,066 + -0.2 1,751,067 - -2.5 1,751,151 - +0.2 1,751,151 - -1.9 1,751,185 - -2.9 1,751,194 + +1.2 1,751,194 + -0.8 1,751,270 - -2.7 1,751,280 + -0.1 1,751,281 - -2.4 1,751,326 + -0.4 1,751,326 + -1.0 1,751,327 - +0.3 1,751,383 - -1.5 1,751,399 - -1.4
Or see this region's nucleotide sequence