Experiment: KB with Trimethoprim 125 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1553 and Psyr_1554 are separated by 424 nucleotides Psyr_1554 and Psyr_1555 are separated by 937 nucleotides
Psyr_1553: Psyr_1553 - hypothetical protein, at 1,746,099 to 1,746,434
_1553
Psyr_1554: Psyr_1554 - hypothetical protein, at 1,746,859 to 1,747,890
_1554
Psyr_1555: Psyr_1555 - Cobyrinic acid a,c-diamide synthase, at 1,748,828 to 1,749,769
_1555
Position (kb)
1746
1747
1748 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1745.875 kb on - strand at 1745.901 kb on + strand at 1745.950 kb on + strand at 1745.950 kb on + strand at 1745.950 kb on + strand at 1745.950 kb on + strand at 1745.950 kb on + strand at 1745.950 kb on + strand at 1745.950 kb on + strand at 1745.951 kb on - strand at 1745.951 kb on - strand at 1745.951 kb on - strand at 1745.951 kb on - strand at 1745.951 kb on - strand at 1746.053 kb on - strand at 1746.056 kb on - strand at 1746.056 kb on - strand at 1746.056 kb on - strand at 1746.056 kb on - strand at 1746.087 kb on + strand at 1746.110 kb on + strand at 1746.158 kb on + strand, within Psyr_1553 at 1746.200 kb on + strand, within Psyr_1553 at 1746.200 kb on + strand, within Psyr_1553 at 1746.201 kb on - strand, within Psyr_1553 at 1746.201 kb on - strand, within Psyr_1553 at 1746.292 kb on + strand, within Psyr_1553 at 1746.348 kb on - strand, within Psyr_1553 at 1746.348 kb on - strand, within Psyr_1553 at 1746.351 kb on + strand, within Psyr_1553 at 1746.351 kb on + strand, within Psyr_1553 at 1746.351 kb on + strand, within Psyr_1553 at 1746.352 kb on - strand, within Psyr_1553 at 1746.352 kb on - strand, within Psyr_1553 at 1746.352 kb on - strand, within Psyr_1553 at 1746.352 kb on - strand, within Psyr_1553 at 1746.354 kb on - strand, within Psyr_1553 at 1746.422 kb on + strand at 1746.423 kb on - strand at 1746.459 kb on + strand at 1746.470 kb on + strand at 1746.533 kb on + strand at 1746.534 kb on - strand at 1746.543 kb on - strand at 1746.620 kb on + strand at 1746.649 kb on + strand at 1746.650 kb on - strand at 1746.650 kb on - strand at 1746.650 kb on - strand at 1746.663 kb on + strand at 1746.694 kb on + strand at 1746.694 kb on + strand at 1746.718 kb on - strand at 1746.739 kb on + strand at 1746.741 kb on + strand at 1746.742 kb on - strand at 1746.780 kb on + strand at 1746.826 kb on + strand at 1746.830 kb on - strand at 1746.834 kb on - strand at 1746.861 kb on - strand at 1746.863 kb on + strand at 1746.863 kb on + strand at 1747.037 kb on - strand, within Psyr_1554 at 1747.084 kb on - strand, within Psyr_1554 at 1747.087 kb on - strand, within Psyr_1554 at 1747.188 kb on + strand, within Psyr_1554 at 1747.220 kb on + strand, within Psyr_1554 at 1747.223 kb on - strand, within Psyr_1554 at 1747.453 kb on + strand, within Psyr_1554 at 1747.454 kb on - strand, within Psyr_1554 at 1747.584 kb on + strand, within Psyr_1554 at 1747.584 kb on + strand, within Psyr_1554 at 1747.584 kb on + strand, within Psyr_1554 at 1747.640 kb on + strand, within Psyr_1554 at 1747.641 kb on - strand, within Psyr_1554 at 1747.755 kb on + strand, within Psyr_1554 at 1747.849 kb on + strand at 1747.849 kb on + strand at 1747.849 kb on + strand at 1747.849 kb on + strand at 1747.849 kb on + strand at 1747.849 kb on + strand at 1747.849 kb on + strand at 1747.849 kb on + strand at 1747.850 kb on - strand at 1747.850 kb on - strand at 1747.893 kb on - strand at 1747.907 kb on + strand at 1747.907 kb on + strand at 1747.912 kb on + strand at 1747.913 kb on - strand at 1747.913 kb on - strand at 1747.937 kb on + strand at 1747.938 kb on - strand at 1747.993 kb on - strand at 1748.080 kb on - strand at 1748.166 kb on + strand at 1748.166 kb on + strand at 1748.167 kb on - strand at 1748.167 kb on - strand at 1748.167 kb on - strand at 1748.200 kb on + strand at 1748.200 kb on + strand at 1748.367 kb on + strand at 1748.401 kb on - strand at 1748.871 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Trimethoprim 125 ug/ml remove 1,745,875 - +0.2 1,745,901 + -0.6 1,745,950 + +0.2 1,745,950 + +1.1 1,745,950 + -0.2 1,745,950 + +0.7 1,745,950 + -1.5 1,745,950 + -2.1 1,745,950 + +0.2 1,745,951 - +0.9 1,745,951 - -0.7 1,745,951 - -0.6 1,745,951 - -0.8 1,745,951 - +1.6 1,746,053 - +0.7 1,746,056 - +1.3 1,746,056 - -0.2 1,746,056 - +1.1 1,746,056 - -0.2 1,746,087 + +0.8 1,746,110 + -0.0 1,746,158 + Psyr_1553 0.18 +0.3 1,746,200 + Psyr_1553 0.30 -0.0 1,746,200 + Psyr_1553 0.30 -0.8 1,746,201 - Psyr_1553 0.30 -0.3 1,746,201 - Psyr_1553 0.30 +1.5 1,746,292 + Psyr_1553 0.57 -0.3 1,746,348 - Psyr_1553 0.74 -1.1 1,746,348 - Psyr_1553 0.74 -1.1 1,746,351 + Psyr_1553 0.75 -0.1 1,746,351 + Psyr_1553 0.75 +0.5 1,746,351 + Psyr_1553 0.75 -0.2 1,746,352 - Psyr_1553 0.75 -1.5 1,746,352 - Psyr_1553 0.75 +0.6 1,746,352 - Psyr_1553 0.75 -1.4 1,746,352 - Psyr_1553 0.75 -0.6 1,746,354 - Psyr_1553 0.76 -2.4 1,746,422 + +0.0 1,746,423 - -0.2 1,746,459 + +0.7 1,746,470 + +1.4 1,746,533 + -1.5 1,746,534 - -0.3 1,746,543 - -2.9 1,746,620 + -0.1 1,746,649 + +1.5 1,746,650 - -2.0 1,746,650 - +0.8 1,746,650 - -1.6 1,746,663 + +0.7 1,746,694 + -0.0 1,746,694 + +0.2 1,746,718 - -2.1 1,746,739 + -1.0 1,746,741 + +0.6 1,746,742 - -2.4 1,746,780 + +1.1 1,746,826 + -0.5 1,746,830 - -0.2 1,746,834 - -2.0 1,746,861 - +0.4 1,746,863 + -0.2 1,746,863 + +0.1 1,747,037 - Psyr_1554 0.17 +0.2 1,747,084 - Psyr_1554 0.22 -0.9 1,747,087 - Psyr_1554 0.22 -0.4 1,747,188 + Psyr_1554 0.32 -0.2 1,747,220 + Psyr_1554 0.35 -0.3 1,747,223 - Psyr_1554 0.35 +1.1 1,747,453 + Psyr_1554 0.58 -1.2 1,747,454 - Psyr_1554 0.58 -0.2 1,747,584 + Psyr_1554 0.70 -0.9 1,747,584 + Psyr_1554 0.70 +0.5 1,747,584 + Psyr_1554 0.70 +1.0 1,747,640 + Psyr_1554 0.76 -1.5 1,747,641 - Psyr_1554 0.76 +0.1 1,747,755 + Psyr_1554 0.87 +0.2 1,747,849 + -1.2 1,747,849 + -1.2 1,747,849 + -0.7 1,747,849 + +0.8 1,747,849 + -1.8 1,747,849 + -1.8 1,747,849 + -2.4 1,747,849 + -2.2 1,747,850 - -0.2 1,747,850 - -0.8 1,747,893 - -0.6 1,747,907 + -3.5 1,747,907 + -3.5 1,747,912 + -1.9 1,747,913 - -1.4 1,747,913 - -1.0 1,747,937 + -1.0 1,747,938 - -1.5 1,747,993 - -0.6 1,748,080 - -0.5 1,748,166 + +0.2 1,748,166 + +0.2 1,748,167 - -1.2 1,748,167 - -0.5 1,748,167 - +0.2 1,748,200 + -1.3 1,748,200 + -0.2 1,748,367 + -1.5 1,748,401 - +0.3 1,748,871 + -0.4
Or see this region's nucleotide sequence