Experiment: KB with Trimethoprim 125 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1550 and Psyr_1551 are separated by 28 nucleotides Psyr_1551 and Psyr_RNA25 are separated by 92 nucleotides Psyr_RNA25 and Psyr_1552 are separated by 58 nucleotides Psyr_1552 and Psyr_1553 are separated by 602 nucleotides
Psyr_1550: Psyr_1550 - Beta-lactamase-like protein, at 1,743,360 to 1,744,118
_1550
Psyr_1551: Psyr_1551 - phosphoribosylaminoimidazole-succinocarboxamide synthase, at 1,744,147 to 1,744,857
_1551
Psyr_RNA25: Psyr_RNA25 - tRNA-Ser, at 1,744,950 to 1,745,039
_RNA25
Psyr_1552: Psyr_1552 - CDS, at 1,745,098 to 1,745,496
_1552
Psyr_1553: Psyr_1553 - hypothetical protein, at 1,746,099 to 1,746,434
_1553
Position (kb)
1745
1746 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1744.109 kb on + strand at 1744.125 kb on + strand at 1744.125 kb on + strand at 1744.125 kb on + strand at 1744.125 kb on + strand at 1744.125 kb on + strand at 1744.129 kb on + strand at 1744.129 kb on + strand at 1744.129 kb on + strand at 1744.129 kb on + strand at 1744.934 kb on + strand at 1744.996 kb on + strand, within Psyr_RNA25 at 1745.012 kb on + strand, within Psyr_RNA25 at 1745.040 kb on + strand at 1745.046 kb on + strand at 1745.047 kb on - strand at 1745.069 kb on + strand at 1745.080 kb on + strand at 1745.092 kb on + strand at 1745.113 kb on + strand at 1745.114 kb on - strand at 1745.187 kb on + strand, within Psyr_1552 at 1745.187 kb on + strand, within Psyr_1552 at 1745.187 kb on + strand, within Psyr_1552 at 1745.187 kb on + strand, within Psyr_1552 at 1745.187 kb on + strand, within Psyr_1552 at 1745.187 kb on + strand, within Psyr_1552 at 1745.188 kb on - strand, within Psyr_1552 at 1745.188 kb on - strand, within Psyr_1552 at 1745.188 kb on - strand, within Psyr_1552 at 1745.188 kb on - strand, within Psyr_1552 at 1745.188 kb on - strand, within Psyr_1552 at 1745.262 kb on + strand, within Psyr_1552 at 1745.262 kb on + strand, within Psyr_1552 at 1745.263 kb on - strand, within Psyr_1552 at 1745.263 kb on - strand, within Psyr_1552 at 1745.263 kb on - strand, within Psyr_1552 at 1745.263 kb on - strand, within Psyr_1552 at 1745.263 kb on - strand, within Psyr_1552 at 1745.269 kb on + strand, within Psyr_1552 at 1745.269 kb on + strand, within Psyr_1552 at 1745.269 kb on + strand, within Psyr_1552 at 1745.269 kb on + strand, within Psyr_1552 at 1745.270 kb on - strand, within Psyr_1552 at 1745.270 kb on - strand, within Psyr_1552 at 1745.270 kb on - strand, within Psyr_1552 at 1745.270 kb on - strand, within Psyr_1552 at 1745.270 kb on - strand, within Psyr_1552 at 1745.270 kb on - strand, within Psyr_1552 at 1745.270 kb on - strand, within Psyr_1552 at 1745.270 kb on - strand, within Psyr_1552 at 1745.270 kb on - strand, within Psyr_1552 at 1745.270 kb on - strand, within Psyr_1552 at 1745.270 kb on - strand, within Psyr_1552 at 1745.270 kb on - strand, within Psyr_1552 at 1745.270 kb on - strand, within Psyr_1552 at 1745.270 kb on - strand, within Psyr_1552 at 1745.278 kb on - strand, within Psyr_1552 at 1745.316 kb on + strand, within Psyr_1552 at 1745.316 kb on + strand, within Psyr_1552 at 1745.317 kb on - strand, within Psyr_1552 at 1745.317 kb on - strand, within Psyr_1552 at 1745.317 kb on - strand, within Psyr_1552 at 1745.498 kb on + strand at 1745.498 kb on + strand at 1745.499 kb on - strand at 1745.499 kb on - strand at 1745.499 kb on - strand at 1745.551 kb on - strand at 1745.551 kb on - strand at 1745.551 kb on - strand at 1745.551 kb on - strand at 1745.584 kb on + strand at 1745.613 kb on + strand at 1745.613 kb on + strand at 1745.614 kb on - strand at 1745.655 kb on + strand at 1745.655 kb on + strand at 1745.700 kb on + strand at 1745.785 kb on - strand at 1745.875 kb on - strand at 1745.901 kb on + strand at 1745.950 kb on + strand at 1745.950 kb on + strand at 1745.950 kb on + strand at 1745.950 kb on + strand at 1745.950 kb on + strand at 1745.950 kb on + strand at 1745.950 kb on + strand at 1745.951 kb on - strand at 1745.951 kb on - strand at 1745.951 kb on - strand at 1745.951 kb on - strand at 1745.951 kb on - strand at 1746.053 kb on - strand at 1746.056 kb on - strand at 1746.056 kb on - strand at 1746.056 kb on - strand at 1746.056 kb on - strand at 1746.087 kb on + strand at 1746.110 kb on + strand at 1746.158 kb on + strand, within Psyr_1553 at 1746.200 kb on + strand, within Psyr_1553 at 1746.200 kb on + strand, within Psyr_1553 at 1746.201 kb on - strand, within Psyr_1553 at 1746.201 kb on - strand, within Psyr_1553 at 1746.292 kb on + strand, within Psyr_1553 at 1746.348 kb on - strand, within Psyr_1553 at 1746.348 kb on - strand, within Psyr_1553 at 1746.351 kb on + strand, within Psyr_1553 at 1746.351 kb on + strand, within Psyr_1553 at 1746.351 kb on + strand, within Psyr_1553 at 1746.352 kb on - strand, within Psyr_1553 at 1746.352 kb on - strand, within Psyr_1553 at 1746.352 kb on - strand, within Psyr_1553 at 1746.352 kb on - strand, within Psyr_1553 at 1746.354 kb on - strand, within Psyr_1553 at 1746.422 kb on + strand at 1746.423 kb on - strand at 1746.459 kb on + strand at 1746.470 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Trimethoprim 125 ug/ml remove 1,744,109 + -1.1 1,744,125 + -1.0 1,744,125 + +0.5 1,744,125 + +0.2 1,744,125 + +0.6 1,744,125 + -1.2 1,744,129 + -1.2 1,744,129 + +0.1 1,744,129 + +3.3 1,744,129 + -1.8 1,744,934 + -1.4 1,744,996 + Psyr_RNA25 0.51 +0.5 1,745,012 + Psyr_RNA25 0.69 +0.8 1,745,040 + +0.0 1,745,046 + +1.1 1,745,047 - -2.0 1,745,069 + -1.0 1,745,080 + +0.2 1,745,092 + -0.1 1,745,113 + -0.8 1,745,114 - +0.4 1,745,187 + Psyr_1552 0.22 +0.2 1,745,187 + Psyr_1552 0.22 -0.5 1,745,187 + Psyr_1552 0.22 +0.4 1,745,187 + Psyr_1552 0.22 +0.1 1,745,187 + Psyr_1552 0.22 +0.4 1,745,187 + Psyr_1552 0.22 -0.3 1,745,188 - Psyr_1552 0.23 +1.3 1,745,188 - Psyr_1552 0.23 -1.2 1,745,188 - Psyr_1552 0.23 -0.2 1,745,188 - Psyr_1552 0.23 -0.8 1,745,188 - Psyr_1552 0.23 -0.5 1,745,262 + Psyr_1552 0.41 +1.0 1,745,262 + Psyr_1552 0.41 -0.1 1,745,263 - Psyr_1552 0.41 -0.1 1,745,263 - Psyr_1552 0.41 -0.6 1,745,263 - Psyr_1552 0.41 -2.1 1,745,263 - Psyr_1552 0.41 -1.8 1,745,263 - Psyr_1552 0.41 +1.8 1,745,269 + Psyr_1552 0.43 -0.2 1,745,269 + Psyr_1552 0.43 +0.8 1,745,269 + Psyr_1552 0.43 -0.8 1,745,269 + Psyr_1552 0.43 -0.5 1,745,270 - Psyr_1552 0.43 +0.5 1,745,270 - Psyr_1552 0.43 +0.8 1,745,270 - Psyr_1552 0.43 +0.5 1,745,270 - Psyr_1552 0.43 +0.7 1,745,270 - Psyr_1552 0.43 +0.8 1,745,270 - Psyr_1552 0.43 -0.8 1,745,270 - Psyr_1552 0.43 -0.5 1,745,270 - Psyr_1552 0.43 +1.3 1,745,270 - Psyr_1552 0.43 -0.8 1,745,270 - Psyr_1552 0.43 +0.3 1,745,270 - Psyr_1552 0.43 +0.8 1,745,270 - Psyr_1552 0.43 -0.3 1,745,270 - Psyr_1552 0.43 -2.0 1,745,270 - Psyr_1552 0.43 +0.4 1,745,278 - Psyr_1552 0.45 +0.2 1,745,316 + Psyr_1552 0.55 -1.7 1,745,316 + Psyr_1552 0.55 -0.4 1,745,317 - Psyr_1552 0.55 +0.8 1,745,317 - Psyr_1552 0.55 +1.1 1,745,317 - Psyr_1552 0.55 -1.0 1,745,498 + -0.5 1,745,498 + -0.5 1,745,499 - -0.5 1,745,499 - -1.1 1,745,499 - -1.2 1,745,551 - +0.2 1,745,551 - +0.4 1,745,551 - -0.5 1,745,551 - -0.4 1,745,584 + -0.6 1,745,613 + -0.2 1,745,613 + -0.4 1,745,614 - +2.5 1,745,655 + -1.9 1,745,655 + -2.5 1,745,700 + -1.2 1,745,785 - +0.5 1,745,875 - +0.2 1,745,901 + -0.6 1,745,950 + +0.2 1,745,950 + +1.1 1,745,950 + -0.2 1,745,950 + +0.7 1,745,950 + -1.5 1,745,950 + -2.1 1,745,950 + +0.2 1,745,951 - +0.9 1,745,951 - -0.7 1,745,951 - -0.6 1,745,951 - -0.8 1,745,951 - +1.6 1,746,053 - +0.7 1,746,056 - +1.3 1,746,056 - -0.2 1,746,056 - +1.1 1,746,056 - -0.2 1,746,087 + +0.8 1,746,110 + -0.0 1,746,158 + Psyr_1553 0.18 +0.3 1,746,200 + Psyr_1553 0.30 -0.0 1,746,200 + Psyr_1553 0.30 -0.8 1,746,201 - Psyr_1553 0.30 -0.3 1,746,201 - Psyr_1553 0.30 +1.5 1,746,292 + Psyr_1553 0.57 -0.3 1,746,348 - Psyr_1553 0.74 -1.1 1,746,348 - Psyr_1553 0.74 -1.1 1,746,351 + Psyr_1553 0.75 -0.1 1,746,351 + Psyr_1553 0.75 +0.5 1,746,351 + Psyr_1553 0.75 -0.2 1,746,352 - Psyr_1553 0.75 -1.5 1,746,352 - Psyr_1553 0.75 +0.6 1,746,352 - Psyr_1553 0.75 -1.4 1,746,352 - Psyr_1553 0.75 -0.6 1,746,354 - Psyr_1553 0.76 -2.4 1,746,422 + +0.0 1,746,423 - -0.2 1,746,459 + +0.7 1,746,470 + +1.4
Or see this region's nucleotide sequence