Experiment: KB with Trimethoprim 125 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1459 and Psyr_1460 are separated by 2 nucleotides Psyr_1460 and Psyr_1461 overlap by 23 nucleotides
Psyr_1459: Psyr_1459 - DNA polymerase, beta-like region, at 1,646,308 to 1,647,093
_1459
Psyr_1460: Psyr_1460 - hypothetical protein, at 1,647,096 to 1,647,725
_1460
Psyr_1461: Psyr_1461 - hypothetical protein, at 1,647,703 to 1,649,067
_1461
Position (kb)
1647
1648 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1646.255 kb on - strand at 1646.255 kb on - strand at 1646.255 kb on - strand at 1646.276 kb on + strand at 1646.319 kb on + strand at 1646.340 kb on - strand at 1646.398 kb on + strand, within Psyr_1459 at 1646.402 kb on + strand, within Psyr_1459 at 1646.403 kb on - strand, within Psyr_1459 at 1646.420 kb on + strand, within Psyr_1459 at 1646.447 kb on + strand, within Psyr_1459 at 1646.462 kb on + strand, within Psyr_1459 at 1646.633 kb on + strand, within Psyr_1459 at 1646.654 kb on - strand, within Psyr_1459 at 1646.835 kb on + strand, within Psyr_1459 at 1646.836 kb on - strand, within Psyr_1459 at 1646.872 kb on + strand, within Psyr_1459 at 1646.873 kb on - strand, within Psyr_1459 at 1646.876 kb on - strand, within Psyr_1459 at 1646.958 kb on + strand at 1647.095 kb on + strand at 1647.096 kb on - strand at 1647.096 kb on - strand at 1647.118 kb on + strand at 1647.122 kb on - strand at 1647.253 kb on - strand at 1647.294 kb on - strand, within Psyr_1460 at 1647.300 kb on + strand, within Psyr_1460 at 1647.324 kb on + strand, within Psyr_1460 at 1647.325 kb on - strand, within Psyr_1460 at 1647.406 kb on + strand at 1647.448 kb on + strand, within Psyr_1460 at 1647.448 kb on + strand, within Psyr_1460 at 1647.478 kb on + strand, within Psyr_1460 at 1647.543 kb on - strand, within Psyr_1460 at 1647.551 kb on - strand, within Psyr_1460 at 1647.565 kb on + strand, within Psyr_1460 at 1647.566 kb on - strand, within Psyr_1460 at 1647.623 kb on - strand, within Psyr_1460 at 1647.623 kb on - strand, within Psyr_1460 at 1647.650 kb on + strand, within Psyr_1460 at 1647.692 kb on + strand at 1647.692 kb on + strand at 1647.693 kb on - strand at 1647.700 kb on + strand at 1647.701 kb on - strand at 1647.701 kb on - strand at 1647.701 kb on - strand at 1647.717 kb on + strand at 1647.718 kb on - strand at 1647.822 kb on + strand at 1647.864 kb on - strand, within Psyr_1461 at 1647.917 kb on - strand, within Psyr_1461 at 1647.950 kb on + strand, within Psyr_1461 at 1648.035 kb on - strand, within Psyr_1461 at 1648.041 kb on + strand, within Psyr_1461 at 1648.042 kb on - strand, within Psyr_1461 at 1648.042 kb on - strand, within Psyr_1461 at 1648.043 kb on + strand, within Psyr_1461 at 1648.043 kb on + strand, within Psyr_1461 at 1648.044 kb on - strand, within Psyr_1461 at 1648.044 kb on - strand, within Psyr_1461 at 1648.069 kb on + strand, within Psyr_1461 at 1648.081 kb on + strand, within Psyr_1461 at 1648.081 kb on + strand, within Psyr_1461 at 1648.082 kb on - strand, within Psyr_1461 at 1648.082 kb on - strand, within Psyr_1461 at 1648.087 kb on - strand, within Psyr_1461 at 1648.088 kb on + strand, within Psyr_1461 at 1648.132 kb on + strand, within Psyr_1461 at 1648.133 kb on - strand, within Psyr_1461 at 1648.133 kb on - strand, within Psyr_1461 at 1648.167 kb on + strand, within Psyr_1461 at 1648.187 kb on - strand, within Psyr_1461 at 1648.263 kb on - strand, within Psyr_1461 at 1648.265 kb on - strand, within Psyr_1461 at 1648.265 kb on - strand, within Psyr_1461 at 1648.328 kb on - strand, within Psyr_1461 at 1648.375 kb on + strand, within Psyr_1461 at 1648.376 kb on - strand, within Psyr_1461 at 1648.536 kb on - strand, within Psyr_1461 at 1648.539 kb on + strand, within Psyr_1461 at 1648.698 kb on + strand, within Psyr_1461
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Trimethoprim 125 ug/ml remove 1,646,255 - -2.4 1,646,255 - +0.2 1,646,255 - -0.8 1,646,276 + -0.9 1,646,319 + -0.4 1,646,340 - -1.3 1,646,398 + Psyr_1459 0.11 -0.8 1,646,402 + Psyr_1459 0.12 -1.2 1,646,403 - Psyr_1459 0.12 +0.8 1,646,420 + Psyr_1459 0.14 +0.8 1,646,447 + Psyr_1459 0.18 +0.2 1,646,462 + Psyr_1459 0.20 -1.4 1,646,633 + Psyr_1459 0.41 -0.7 1,646,654 - Psyr_1459 0.44 -2.8 1,646,835 + Psyr_1459 0.67 -0.8 1,646,836 - Psyr_1459 0.67 -0.8 1,646,872 + Psyr_1459 0.72 -0.4 1,646,873 - Psyr_1459 0.72 -0.6 1,646,876 - Psyr_1459 0.72 -0.5 1,646,958 + -1.2 1,647,095 + -1.3 1,647,096 - -2.4 1,647,096 - -2.0 1,647,118 + -0.5 1,647,122 - -0.2 1,647,253 - +0.8 1,647,294 - Psyr_1460 0.31 -2.2 1,647,300 + Psyr_1460 0.32 -1.8 1,647,324 + Psyr_1460 0.36 +0.1 1,647,325 - Psyr_1460 0.36 -2.2 1,647,406 + -0.8 1,647,448 + Psyr_1460 0.56 +0.2 1,647,448 + Psyr_1460 0.56 +0.3 1,647,478 + Psyr_1460 0.61 -1.0 1,647,543 - Psyr_1460 0.71 -0.3 1,647,551 - Psyr_1460 0.72 -0.8 1,647,565 + Psyr_1460 0.74 -2.4 1,647,566 - Psyr_1460 0.75 -0.5 1,647,623 - Psyr_1460 0.84 -0.7 1,647,623 - Psyr_1460 0.84 +0.4 1,647,650 + Psyr_1460 0.88 -1.7 1,647,692 + -2.1 1,647,692 + -0.4 1,647,693 - -0.7 1,647,700 + -0.8 1,647,701 - +0.1 1,647,701 - -0.9 1,647,701 - -1.4 1,647,717 + -2.2 1,647,718 - -1.9 1,647,822 + -0.4 1,647,864 - Psyr_1461 0.12 +1.1 1,647,917 - Psyr_1461 0.16 -0.0 1,647,950 + Psyr_1461 0.18 +0.8 1,648,035 - Psyr_1461 0.24 +0.6 1,648,041 + Psyr_1461 0.25 -1.4 1,648,042 - Psyr_1461 0.25 +0.6 1,648,042 - Psyr_1461 0.25 +2.1 1,648,043 + Psyr_1461 0.25 -2.4 1,648,043 + Psyr_1461 0.25 -2.5 1,648,044 - Psyr_1461 0.25 +0.7 1,648,044 - Psyr_1461 0.25 -0.3 1,648,069 + Psyr_1461 0.27 -0.2 1,648,081 + Psyr_1461 0.28 -1.5 1,648,081 + Psyr_1461 0.28 -2.0 1,648,082 - Psyr_1461 0.28 +0.8 1,648,082 - Psyr_1461 0.28 +2.5 1,648,087 - Psyr_1461 0.28 +0.1 1,648,088 + Psyr_1461 0.28 -1.2 1,648,132 + Psyr_1461 0.31 -0.5 1,648,133 - Psyr_1461 0.32 +0.3 1,648,133 - Psyr_1461 0.32 -1.2 1,648,167 + Psyr_1461 0.34 -0.0 1,648,187 - Psyr_1461 0.35 -1.5 1,648,263 - Psyr_1461 0.41 +0.3 1,648,265 - Psyr_1461 0.41 +1.1 1,648,265 - Psyr_1461 0.41 -0.5 1,648,328 - Psyr_1461 0.46 -0.8 1,648,375 + Psyr_1461 0.49 -0.3 1,648,376 - Psyr_1461 0.49 -1.2 1,648,536 - Psyr_1461 0.61 -0.5 1,648,539 + Psyr_1461 0.61 -1.9 1,648,698 + Psyr_1461 0.73 -0.9
Or see this region's nucleotide sequence