Experiment: KB with Trimethoprim 125 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1416 and Psyr_1417 are separated by 78 nucleotides Psyr_1417 and Psyr_1418 are separated by 171 nucleotides Psyr_1418 and Psyr_1419 are separated by 1 nucleotides Psyr_1419 and Psyr_RNA23 are separated by 63 nucleotides Psyr_RNA23 and Psyr_RNA24 are separated by 95 nucleotides
Psyr_1416: Psyr_1416 - OmpA/MotB, at 1,601,417 to 1,601,920
_1416
Psyr_1417: Psyr_1417 - TPR repeat protein, at 1,601,999 to 1,602,745
_1417
Psyr_1418: Psyr_1418 - Radical SAM, at 1,602,917 to 1,603,564
_1418
Psyr_1419: Psyr_1419 - preQ(0) biosynthesis protein QueC, at 1,603,566 to 1,604,255
_1419
Psyr_RNA23: Psyr_RNA23 - tRNA-Lys, at 1,604,319 to 1,604,394
_RNA23
Psyr_RNA24: Psyr_RNA24 - tRNA-Pro, at 1,604,490 to 1,604,566
_RNA24
Position (kb)
1602
1603
1604 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1601.918 kb on + strand at 1601.918 kb on + strand at 1602.241 kb on + strand, within Psyr_1417 at 1602.241 kb on + strand, within Psyr_1417 at 1602.241 kb on + strand, within Psyr_1417 at 1602.241 kb on + strand, within Psyr_1417 at 1602.242 kb on - strand, within Psyr_1417 at 1602.242 kb on - strand, within Psyr_1417 at 1602.242 kb on - strand, within Psyr_1417 at 1602.242 kb on - strand, within Psyr_1417 at 1602.242 kb on - strand, within Psyr_1417 at 1602.242 kb on - strand, within Psyr_1417 at 1602.242 kb on - strand, within Psyr_1417 at 1602.242 kb on - strand, within Psyr_1417 at 1602.242 kb on - strand, within Psyr_1417 at 1602.242 kb on - strand, within Psyr_1417 at 1602.242 kb on - strand, within Psyr_1417 at 1602.242 kb on - strand, within Psyr_1417 at 1602.242 kb on - strand, within Psyr_1417 at 1602.274 kb on + strand, within Psyr_1417 at 1602.275 kb on - strand, within Psyr_1417 at 1602.389 kb on + strand, within Psyr_1417 at 1602.390 kb on - strand, within Psyr_1417 at 1602.470 kb on + strand, within Psyr_1417 at 1602.471 kb on - strand, within Psyr_1417 at 1602.486 kb on - strand, within Psyr_1417 at 1602.614 kb on + strand, within Psyr_1417 at 1602.614 kb on + strand, within Psyr_1417 at 1602.615 kb on - strand, within Psyr_1417 at 1602.669 kb on + strand, within Psyr_1417 at 1602.669 kb on + strand, within Psyr_1417 at 1602.670 kb on - strand, within Psyr_1417 at 1602.778 kb on - strand at 1602.828 kb on + strand at 1602.894 kb on + strand at 1602.894 kb on + strand at 1602.894 kb on + strand at 1602.894 kb on + strand at 1602.930 kb on + strand at 1602.930 kb on + strand at 1602.993 kb on + strand, within Psyr_1418 at 1603.118 kb on + strand, within Psyr_1418 at 1603.118 kb on + strand, within Psyr_1418 at 1603.119 kb on - strand, within Psyr_1418 at 1603.203 kb on + strand, within Psyr_1418 at 1603.203 kb on + strand, within Psyr_1418 at 1603.204 kb on - strand, within Psyr_1418 at 1603.310 kb on + strand, within Psyr_1418 at 1603.377 kb on - strand, within Psyr_1418 at 1603.407 kb on - strand, within Psyr_1418 at 1603.565 kb on + strand at 1603.860 kb on + strand, within Psyr_1419 at 1603.860 kb on + strand, within Psyr_1419 at 1603.860 kb on + strand, within Psyr_1419 at 1603.860 kb on + strand, within Psyr_1419 at 1603.860 kb on + strand, within Psyr_1419 at 1603.860 kb on + strand, within Psyr_1419 at 1603.860 kb on + strand, within Psyr_1419 at 1603.861 kb on - strand, within Psyr_1419 at 1603.861 kb on - strand, within Psyr_1419 at 1603.861 kb on - strand, within Psyr_1419 at 1603.861 kb on - strand, within Psyr_1419 at 1604.299 kb on + strand at 1604.301 kb on + strand at 1604.301 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Trimethoprim 125 ug/ml remove 1,601,918 + -1.5 1,601,918 + -0.5 1,602,241 + Psyr_1417 0.32 -1.2 1,602,241 + Psyr_1417 0.32 -1.4 1,602,241 + Psyr_1417 0.32 +2.8 1,602,241 + Psyr_1417 0.32 -0.6 1,602,242 - Psyr_1417 0.33 -0.5 1,602,242 - Psyr_1417 0.33 -2.0 1,602,242 - Psyr_1417 0.33 +0.1 1,602,242 - Psyr_1417 0.33 -0.8 1,602,242 - Psyr_1417 0.33 -0.2 1,602,242 - Psyr_1417 0.33 -1.8 1,602,242 - Psyr_1417 0.33 -1.5 1,602,242 - Psyr_1417 0.33 -1.2 1,602,242 - Psyr_1417 0.33 -1.3 1,602,242 - Psyr_1417 0.33 -1.6 1,602,242 - Psyr_1417 0.33 -2.4 1,602,242 - Psyr_1417 0.33 -0.5 1,602,242 - Psyr_1417 0.33 -2.0 1,602,274 + Psyr_1417 0.37 -0.2 1,602,275 - Psyr_1417 0.37 -2.8 1,602,389 + Psyr_1417 0.52 +0.3 1,602,390 - Psyr_1417 0.52 +0.4 1,602,470 + Psyr_1417 0.63 +0.3 1,602,471 - Psyr_1417 0.63 +1.1 1,602,486 - Psyr_1417 0.65 -0.8 1,602,614 + Psyr_1417 0.82 +0.5 1,602,614 + Psyr_1417 0.82 -0.5 1,602,615 - Psyr_1417 0.82 -1.4 1,602,669 + Psyr_1417 0.90 +1.7 1,602,669 + Psyr_1417 0.90 +0.6 1,602,670 - Psyr_1417 0.90 -0.3 1,602,778 - -0.7 1,602,828 + +2.0 1,602,894 + +0.4 1,602,894 + -1.5 1,602,894 + +0.8 1,602,894 + +0.8 1,602,930 + -0.6 1,602,930 + +0.4 1,602,993 + Psyr_1418 0.12 -0.2 1,603,118 + Psyr_1418 0.31 -0.8 1,603,118 + Psyr_1418 0.31 -1.1 1,603,119 - Psyr_1418 0.31 -0.5 1,603,203 + Psyr_1418 0.44 -2.5 1,603,203 + Psyr_1418 0.44 -1.0 1,603,204 - Psyr_1418 0.44 -0.2 1,603,310 + Psyr_1418 0.61 -0.5 1,603,377 - Psyr_1418 0.71 -1.6 1,603,407 - Psyr_1418 0.76 -1.8 1,603,565 + -1.9 1,603,860 + Psyr_1419 0.43 +0.4 1,603,860 + Psyr_1419 0.43 +0.2 1,603,860 + Psyr_1419 0.43 +0.1 1,603,860 + Psyr_1419 0.43 +0.2 1,603,860 + Psyr_1419 0.43 +1.8 1,603,860 + Psyr_1419 0.43 +0.4 1,603,860 + Psyr_1419 0.43 -0.2 1,603,861 - Psyr_1419 0.43 -1.8 1,603,861 - Psyr_1419 0.43 -0.8 1,603,861 - Psyr_1419 0.43 +0.8 1,603,861 - Psyr_1419 0.43 -1.2 1,604,299 + -0.2 1,604,301 + +1.1 1,604,301 + -0.3
Or see this region's nucleotide sequence