Experiment: KB with Trimethoprim 125 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1110 and Psyr_1111 are separated by 50 nucleotides Psyr_1111 and Psyr_1112 are separated by 16 nucleotides Psyr_1112 and Psyr_1113 are separated by 169 nucleotides
Psyr_1110: Psyr_1110 - glucokinase, at 1,257,902 to 1,258,867
_1110
Psyr_1111: Psyr_1111 - Response regulator receiver:Transcriptional regulatory protein, C-terminal, at 1,258,918 to 1,259,655
_1111
Psyr_1112: Psyr_1112 - ATP-binding region, ATPase-like:Histidine kinase, HAMP region:Histidine kinase A, N-terminal, at 1,259,672 to 1,261,123
_1112
Psyr_1113: Psyr_1113 - glucose-binding protein, at 1,261,293 to 1,262,588
_1113
Position (kb)
1259
1260
1261
1262 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1258.676 kb on + strand, within Psyr_1110 at 1258.676 kb on + strand, within Psyr_1110 at 1258.676 kb on + strand, within Psyr_1110 at 1258.677 kb on - strand, within Psyr_1110 at 1258.677 kb on - strand, within Psyr_1110 at 1258.677 kb on - strand, within Psyr_1110 at 1258.677 kb on - strand, within Psyr_1110 at 1258.677 kb on - strand, within Psyr_1110 at 1258.677 kb on - strand, within Psyr_1110 at 1258.677 kb on - strand, within Psyr_1110 at 1258.677 kb on - strand, within Psyr_1110 at 1258.679 kb on - strand, within Psyr_1110 at 1258.706 kb on - strand, within Psyr_1110 at 1258.769 kb on + strand, within Psyr_1110 at 1258.808 kb on + strand at 1258.879 kb on + strand at 1258.880 kb on - strand at 1258.880 kb on - strand at 1258.933 kb on - strand at 1258.987 kb on + strand at 1258.987 kb on + strand at 1258.987 kb on + strand at 1258.987 kb on + strand at 1258.987 kb on + strand at 1258.987 kb on + strand at 1258.987 kb on + strand at 1258.987 kb on + strand at 1258.987 kb on + strand at 1258.988 kb on - strand at 1258.988 kb on - strand at 1258.988 kb on - strand at 1258.988 kb on - strand at 1259.230 kb on + strand, within Psyr_1111 at 1259.231 kb on - strand, within Psyr_1111 at 1259.589 kb on + strand at 1259.653 kb on + strand at 1259.654 kb on - strand at 1259.654 kb on - strand at 1259.787 kb on + strand at 1259.787 kb on + strand at 1259.787 kb on + strand at 1259.787 kb on + strand at 1259.787 kb on + strand at 1259.788 kb on - strand at 1259.788 kb on - strand at 1259.788 kb on - strand at 1259.788 kb on - strand at 1259.861 kb on + strand, within Psyr_1112 at 1259.910 kb on + strand, within Psyr_1112 at 1259.910 kb on + strand, within Psyr_1112 at 1259.918 kb on - strand, within Psyr_1112 at 1260.143 kb on + strand, within Psyr_1112 at 1260.218 kb on + strand, within Psyr_1112 at 1260.218 kb on + strand, within Psyr_1112 at 1260.218 kb on + strand, within Psyr_1112 at 1260.218 kb on + strand, within Psyr_1112 at 1260.219 kb on - strand, within Psyr_1112 at 1260.219 kb on - strand, within Psyr_1112 at 1260.219 kb on - strand, within Psyr_1112 at 1260.246 kb on - strand, within Psyr_1112 at 1260.246 kb on - strand, within Psyr_1112 at 1260.246 kb on - strand, within Psyr_1112 at 1260.270 kb on + strand, within Psyr_1112 at 1260.270 kb on + strand, within Psyr_1112 at 1260.685 kb on + strand, within Psyr_1112 at 1260.685 kb on + strand, within Psyr_1112 at 1260.791 kb on + strand, within Psyr_1112 at 1260.855 kb on - strand, within Psyr_1112 at 1261.100 kb on + strand at 1261.100 kb on + strand at 1261.100 kb on + strand at 1261.100 kb on + strand at 1261.101 kb on - strand at 1261.101 kb on - strand at 1261.166 kb on + strand at 1261.167 kb on - strand at 1261.270 kb on + strand at 1261.270 kb on + strand at 1261.270 kb on + strand at 1261.270 kb on + strand at 1261.271 kb on - strand at 1261.271 kb on - strand at 1261.271 kb on - strand at 1261.698 kb on + strand, within Psyr_1113 at 1261.699 kb on - strand, within Psyr_1113 at 1261.699 kb on - strand, within Psyr_1113 at 1261.710 kb on + strand, within Psyr_1113 at 1261.711 kb on - strand, within Psyr_1113 at 1261.802 kb on + strand, within Psyr_1113 at 1261.938 kb on + strand, within Psyr_1113 at 1262.028 kb on + strand, within Psyr_1113 at 1262.028 kb on + strand, within Psyr_1113 at 1262.028 kb on + strand, within Psyr_1113 at 1262.028 kb on + strand, within Psyr_1113 at 1262.028 kb on + strand, within Psyr_1113 at 1262.028 kb on + strand, within Psyr_1113 at 1262.028 kb on + strand, within Psyr_1113 at 1262.028 kb on + strand, within Psyr_1113 at 1262.028 kb on + strand, within Psyr_1113 at 1262.028 kb on + strand, within Psyr_1113 at 1262.028 kb on + strand, within Psyr_1113 at 1262.028 kb on + strand, within Psyr_1113 at 1262.028 kb on + strand, within Psyr_1113 at 1262.028 kb on + strand, within Psyr_1113 at 1262.028 kb on + strand, within Psyr_1113 at 1262.029 kb on - strand, within Psyr_1113 at 1262.029 kb on - strand, within Psyr_1113 at 1262.029 kb on - strand, within Psyr_1113 at 1262.029 kb on - strand, within Psyr_1113 at 1262.029 kb on - strand, within Psyr_1113 at 1262.029 kb on - strand, within Psyr_1113 at 1262.029 kb on - strand, within Psyr_1113 at 1262.029 kb on - strand, within Psyr_1113
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Trimethoprim 125 ug/ml remove 1,258,676 + Psyr_1110 0.80 -0.8 1,258,676 + Psyr_1110 0.80 +0.4 1,258,676 + Psyr_1110 0.80 +0.5 1,258,677 - Psyr_1110 0.80 -0.4 1,258,677 - Psyr_1110 0.80 +0.4 1,258,677 - Psyr_1110 0.80 -2.5 1,258,677 - Psyr_1110 0.80 -1.0 1,258,677 - Psyr_1110 0.80 +0.8 1,258,677 - Psyr_1110 0.80 -1.2 1,258,677 - Psyr_1110 0.80 -0.1 1,258,677 - Psyr_1110 0.80 -0.0 1,258,679 - Psyr_1110 0.80 +0.2 1,258,706 - Psyr_1110 0.83 -0.7 1,258,769 + Psyr_1110 0.90 -0.0 1,258,808 + -0.3 1,258,879 + -0.7 1,258,880 - -0.7 1,258,880 - -0.4 1,258,933 - -0.6 1,258,987 + +0.8 1,258,987 + +0.7 1,258,987 + +1.8 1,258,987 + +0.1 1,258,987 + -0.1 1,258,987 + -0.0 1,258,987 + -1.5 1,258,987 + -1.0 1,258,987 + +0.2 1,258,988 - -2.5 1,258,988 - +0.3 1,258,988 - -0.4 1,258,988 - -0.0 1,259,230 + Psyr_1111 0.42 -0.8 1,259,231 - Psyr_1111 0.42 +0.2 1,259,589 + -0.3 1,259,653 + +0.5 1,259,654 - +0.1 1,259,654 - +0.2 1,259,787 + +0.2 1,259,787 + -0.0 1,259,787 + +0.8 1,259,787 + -0.3 1,259,787 + -1.0 1,259,788 - -0.9 1,259,788 - -0.3 1,259,788 - -0.2 1,259,788 - -0.7 1,259,861 + Psyr_1112 0.13 +0.4 1,259,910 + Psyr_1112 0.16 +0.9 1,259,910 + Psyr_1112 0.16 +0.1 1,259,918 - Psyr_1112 0.17 +0.6 1,260,143 + Psyr_1112 0.32 +0.7 1,260,218 + Psyr_1112 0.38 -0.9 1,260,218 + Psyr_1112 0.38 +0.4 1,260,218 + Psyr_1112 0.38 +1.8 1,260,218 + Psyr_1112 0.38 +0.8 1,260,219 - Psyr_1112 0.38 -0.2 1,260,219 - Psyr_1112 0.38 +0.8 1,260,219 - Psyr_1112 0.38 +0.8 1,260,246 - Psyr_1112 0.40 +0.7 1,260,246 - Psyr_1112 0.40 +0.0 1,260,246 - Psyr_1112 0.40 +0.9 1,260,270 + Psyr_1112 0.41 -0.2 1,260,270 + Psyr_1112 0.41 +0.1 1,260,685 + Psyr_1112 0.70 +0.8 1,260,685 + Psyr_1112 0.70 -1.5 1,260,791 + Psyr_1112 0.77 -0.0 1,260,855 - Psyr_1112 0.81 +0.5 1,261,100 + +0.3 1,261,100 + +0.8 1,261,100 + +1.7 1,261,100 + +1.0 1,261,101 - -2.0 1,261,101 - +0.3 1,261,166 + -0.8 1,261,167 - +0.6 1,261,270 + -1.5 1,261,270 + +0.4 1,261,270 + +0.6 1,261,270 + -0.8 1,261,271 - -0.8 1,261,271 - +1.8 1,261,271 - -0.6 1,261,698 + Psyr_1113 0.31 +0.3 1,261,699 - Psyr_1113 0.31 -0.3 1,261,699 - Psyr_1113 0.31 -0.2 1,261,710 + Psyr_1113 0.32 +1.0 1,261,711 - Psyr_1113 0.32 +0.8 1,261,802 + Psyr_1113 0.39 +0.2 1,261,938 + Psyr_1113 0.50 +0.8 1,262,028 + Psyr_1113 0.57 -0.3 1,262,028 + Psyr_1113 0.57 +0.5 1,262,028 + Psyr_1113 0.57 -0.2 1,262,028 + Psyr_1113 0.57 -0.2 1,262,028 + Psyr_1113 0.57 +0.6 1,262,028 + Psyr_1113 0.57 -1.8 1,262,028 + Psyr_1113 0.57 -0.1 1,262,028 + Psyr_1113 0.57 -0.5 1,262,028 + Psyr_1113 0.57 +1.3 1,262,028 + Psyr_1113 0.57 +0.8 1,262,028 + Psyr_1113 0.57 +0.6 1,262,028 + Psyr_1113 0.57 +1.8 1,262,028 + Psyr_1113 0.57 +1.4 1,262,028 + Psyr_1113 0.57 +0.9 1,262,028 + Psyr_1113 0.57 +1.0 1,262,029 - Psyr_1113 0.57 +0.8 1,262,029 - Psyr_1113 0.57 -0.2 1,262,029 - Psyr_1113 0.57 -0.3 1,262,029 - Psyr_1113 0.57 +1.0 1,262,029 - Psyr_1113 0.57 -1.5 1,262,029 - Psyr_1113 0.57 +0.5 1,262,029 - Psyr_1113 0.57 -1.1 1,262,029 - Psyr_1113 0.57 -0.6
Or see this region's nucleotide sequence