Experiment: KB with Trimethoprim 125 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1088 and Psyr_1089 are separated by 43 nucleotides Psyr_1089 and Psyr_1090 are separated by 7 nucleotides Psyr_1090 and Psyr_1091 are separated by 57 nucleotides Psyr_1091 and Psyr_1092 are separated by 319 nucleotides
Psyr_1088: Psyr_1088 - CDS, at 1,230,953 to 1,231,684
_1088
Psyr_1089: Psyr_1089 - conserved hypothetical protein, at 1,231,728 to 1,232,162
_1089
Psyr_1090: Psyr_1090 - DNA polymerase III, chi subunit, at 1,232,170 to 1,232,598
_1090
Psyr_1091: Psyr_1091 - aminopeptidase A, Metallo peptidase, MEROPS family M17, at 1,232,656 to 1,234,146
_1091
Psyr_1092: Psyr_1092 - permease YjgP/YjgQ, at 1,234,466 to 1,235,593
_1092
Position (kb) 1232
1233
1234
1235 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 1231.657 kb on + strand at 1231.675 kb on + strand at 1231.675 kb on + strand at 1231.676 kb on - strand at 1231.676 kb on - strand at 1231.694 kb on + strand at 1231.694 kb on + strand at 1231.694 kb on + strand at 1231.694 kb on + strand at 1231.694 kb on + strand at 1231.695 kb on - strand at 1231.695 kb on - strand at 1231.695 kb on - strand at 1231.695 kb on - strand at 1231.695 kb on - strand at 1231.695 kb on - strand at 1231.704 kb on + strand at 1231.991 kb on - strand, within Psyr_1089 at 1232.156 kb on + strand at 1232.156 kb on + strand at 1232.157 kb on - strand at 1232.157 kb on - strand at 1232.158 kb on - strand at 1232.251 kb on + strand, within Psyr_1090 at 1232.251 kb on + strand, within Psyr_1090 at 1232.251 kb on + strand, within Psyr_1090 at 1232.252 kb on - strand, within Psyr_1090 at 1232.252 kb on - strand, within Psyr_1090 at 1232.492 kb on + strand, within Psyr_1090 at 1232.492 kb on + strand, within Psyr_1090 at 1232.492 kb on + strand, within Psyr_1090 at 1232.492 kb on + strand, within Psyr_1090 at 1232.492 kb on + strand, within Psyr_1090 at 1232.492 kb on + strand, within Psyr_1090 at 1232.492 kb on + strand, within Psyr_1090 at 1232.492 kb on + strand, within Psyr_1090 at 1232.579 kb on + strand at 1232.579 kb on + strand at 1232.579 kb on + strand at 1232.579 kb on + strand at 1232.580 kb on - strand at 1232.580 kb on - strand at 1232.580 kb on - strand at 1232.580 kb on - strand at 1232.580 kb on - strand at 1232.580 kb on - strand at 1232.580 kb on - strand at 1232.657 kb on + strand at 1232.657 kb on + strand at 1232.658 kb on - strand at 1232.681 kb on + strand at 1232.682 kb on - strand at 1232.682 kb on - strand at 1232.882 kb on + strand, within Psyr_1091 at 1232.882 kb on + strand, within Psyr_1091 at 1233.065 kb on + strand, within Psyr_1091 at 1233.395 kb on + strand, within Psyr_1091 at 1233.396 kb on - strand, within Psyr_1091 at 1233.396 kb on - strand, within Psyr_1091 at 1233.548 kb on + strand, within Psyr_1091 at 1233.548 kb on + strand, within Psyr_1091 at 1233.549 kb on - strand, within Psyr_1091 at 1233.735 kb on - strand, within Psyr_1091 at 1233.735 kb on - strand, within Psyr_1091 at 1233.735 kb on - strand, within Psyr_1091 at 1233.735 kb on - strand, within Psyr_1091 at 1233.779 kb on + strand, within Psyr_1091 at 1233.779 kb on + strand, within Psyr_1091 at 1233.779 kb on + strand, within Psyr_1091 at 1233.779 kb on + strand, within Psyr_1091 at 1233.780 kb on - strand, within Psyr_1091 at 1233.780 kb on - strand, within Psyr_1091 at 1233.780 kb on - strand, within Psyr_1091 at 1234.066 kb on + strand at 1234.067 kb on - strand at 1234.132 kb on - strand at 1234.132 kb on - strand at 1234.161 kb on + strand at 1234.162 kb on - strand at 1234.180 kb on + strand at 1234.180 kb on + strand at 1234.181 kb on - strand at 1234.181 kb on - strand at 1234.191 kb on + strand at 1234.191 kb on + strand at 1234.299 kb on + strand at 1234.300 kb on - strand at 1234.300 kb on - strand at 1234.300 kb on - strand at 1234.310 kb on + strand at 1234.311 kb on - strand at 1234.311 kb on - strand at 1234.311 kb on - strand at 1234.350 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Trimethoprim 125 ug/ml remove 1,231,657 + +0.7 1,231,675 + +1.0 1,231,675 + +0.5 1,231,676 - -1.8 1,231,676 - -0.4 1,231,694 + -1.0 1,231,694 + +0.7 1,231,694 + +0.2 1,231,694 + +1.5 1,231,694 + -0.1 1,231,695 - +0.3 1,231,695 - +1.7 1,231,695 - -0.1 1,231,695 - -1.4 1,231,695 - -1.2 1,231,695 - +1.9 1,231,704 + +2.8 1,231,991 - Psyr_1089 0.60 +0.3 1,232,156 + -0.1 1,232,156 + +0.3 1,232,157 - -1.5 1,232,157 - +0.5 1,232,158 - -1.0 1,232,251 + Psyr_1090 0.19 -0.3 1,232,251 + Psyr_1090 0.19 -0.0 1,232,251 + Psyr_1090 0.19 -0.9 1,232,252 - Psyr_1090 0.19 -0.5 1,232,252 - Psyr_1090 0.19 -0.8 1,232,492 + Psyr_1090 0.75 -0.7 1,232,492 + Psyr_1090 0.75 +0.2 1,232,492 + Psyr_1090 0.75 +0.0 1,232,492 + Psyr_1090 0.75 -0.0 1,232,492 + Psyr_1090 0.75 -0.5 1,232,492 + Psyr_1090 0.75 +1.8 1,232,492 + Psyr_1090 0.75 -0.4 1,232,492 + Psyr_1090 0.75 +1.3 1,232,579 + -0.2 1,232,579 + +1.0 1,232,579 + -0.4 1,232,579 + -0.1 1,232,580 - +1.1 1,232,580 - -0.9 1,232,580 - -0.0 1,232,580 - -1.5 1,232,580 - -1.0 1,232,580 - -0.1 1,232,580 - +0.0 1,232,657 + +1.4 1,232,657 + -1.2 1,232,658 - +0.3 1,232,681 + +0.5 1,232,682 - +0.2 1,232,682 - -1.3 1,232,882 + Psyr_1091 0.15 -2.2 1,232,882 + Psyr_1091 0.15 -0.4 1,233,065 + Psyr_1091 0.27 -0.2 1,233,395 + Psyr_1091 0.50 -0.2 1,233,396 - Psyr_1091 0.50 -0.2 1,233,396 - Psyr_1091 0.50 -0.8 1,233,548 + Psyr_1091 0.60 -0.9 1,233,548 + Psyr_1091 0.60 -0.4 1,233,549 - Psyr_1091 0.60 -1.0 1,233,735 - Psyr_1091 0.72 -2.4 1,233,735 - Psyr_1091 0.72 +2.1 1,233,735 - Psyr_1091 0.72 -1.6 1,233,735 - Psyr_1091 0.72 +1.2 1,233,779 + Psyr_1091 0.75 -0.2 1,233,779 + Psyr_1091 0.75 +0.4 1,233,779 + Psyr_1091 0.75 -2.7 1,233,779 + Psyr_1091 0.75 -0.2 1,233,780 - Psyr_1091 0.75 -0.6 1,233,780 - Psyr_1091 0.75 +0.6 1,233,780 - Psyr_1091 0.75 +0.2 1,234,066 + -0.2 1,234,067 - +0.1 1,234,132 - +0.8 1,234,132 - -0.9 1,234,161 + +2.0 1,234,162 - -0.2 1,234,180 + -0.2 1,234,180 + -2.2 1,234,181 - -0.9 1,234,181 - -2.6 1,234,191 + -2.0 1,234,191 + -0.7 1,234,299 + +0.5 1,234,300 - +1.2 1,234,300 - -1.2 1,234,300 - -0.0 1,234,310 + -0.1 1,234,311 - -0.4 1,234,311 - +0.9 1,234,311 - -1.1 1,234,350 + +0.4
Or see this region's nucleotide sequence